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1.
Root Endodermis and Exodermis: Structure, Function, and Responses to the Environment 总被引:9,自引:0,他引:9
Daryl E. Enstone Carol A. Peterson Fengshan Ma 《Journal of Plant Growth Regulation》2003,21(4):335-351
Roots of virtually all vascular plants have an endodermis with a Casparian band, and the majority of angiosperm roots tested also have an exodermis with a Casparian band. Both the endodermis and exodermis may develop suberin lamellae and thick, tertiary walls. Each of these wall modifications has its own function(s). The endodermal Casparian band prevents the unimpeded movement of apoplastic substances into the stele and also prevents the backflow of ions that have moved into the stele symplastically and then were released into its apoplast. In roots with a mature exodermis, the barrier to apoplastic inflow of ions occurs near the root surface, but prevention of backflow of ions from the stele remains a function of the endodermis. The suberin lamellae protect against pathogen invasion and possibly root drying during times of stress. Tertiary walls of the endodermis and exodermis are believed to function in mechanical support of the root, but this idea remains to be tested. During stress, root growth rates decline, and the endodermis and exodermis develop closer to the root tip. In two cases, stress is known to induce the formation of an exodermis, and in several other cases to accelerate the development of both the exodermis and endodermis. The responses of the endodermis and exodermis to drought, exposure to moist air, flooding, salinity, ion deficiency, acidity, and mechanical impedance are discussed. 相似文献
2.
Daryl E. Enstone Carol A. Peterson Fengshan Ma 《Journal of Plant Growth Regulation》2002,21(4):335-351
Roots of virtually all vascular plants have an endodermis with a Casparian band, and the majority of angiosperm roots tested also have an exodermis with a Casparian band. Both the endodermis and exodermis may develop suberin lamellae and thick, tertiary walls. Each of these wall modifications has its own function(s). The endodermal Casparian band prevents the unimpeded movement of apoplastic substances into the stele and also prevents the backflow of ions that have moved into the stele symplastically and then were released into its apoplast. In roots with a mature exodermis, the barrier to apoplastic inflow of ions occurs near the root surface, but prevention of backflow of ions from the stele remains a function of the endodermis. The suberin lamellae protect against pathogen invasion and possibly root drying during times of stress. Tertiary walls of the endodermis and exodermis are believed to function in mechanical support of the root, but this idea remains to be tested. During stress, root growth rates decline, and the endodermis and exodermis develop closer to the root tip. In two cases, stress is known to induce the formation of an exodermis, and in several other cases to accelerate the development of both the exodermis and endodermis. The responses of the endodermis and exodermis to drought, exposure to moist air, flooding, salinity, ion deficiency, acidity, and mechanical impedance are discussed. 相似文献
3.
Evidence for symplastic involvement in the radial movement of calcium in onion roots 总被引:3,自引:0,他引:3
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The pathway of Ca(2+) movement from the soil solution into the stele of the root is not known with certainty despite a considerable body of literature on the subject. Does this ion cross an intact, mature exodermis and endodermis? If so, is its movement through these layers primarily apoplastic or symplastic? These questions were addressed using onion (Allium cepa) adventitious roots lacking laterals. Radioactive Ca(2+) applied to the root tip was not transported to the remainder of the plant, indicating that this ion cannot be supplied to the shoot through this region where the exodermis and endodermis are immature. A more mature zone, in which the endodermal Casparian band was present, delivered 2.67 nmol of Ca(2+) mm(-1) treated root length d(-1) to the transpiration stream, demonstrating that the ion had moved through an intact endodermis. Farther from the root tip, a third zone in which Casparian bands were present in the exodermis as well as the endodermis delivered 0.87 nmol Ca(2+) mm(-1) root length d(-1) to the transpiration stream, proving that the ion had moved through an unbroken exodermis. Compartmental elution analyses indicated that Ca(2+) had not diffused through the Casparian bands of the exodermis, and inhibitor studies using La(3+) and vanadate (VO(4)(3-)) pointed to a major involvement of the symplast in the radial transport of Ca(2+) through the endodermis. It was concluded that in onion roots, the radial movement of Ca(2+) through the exodermis and endodermis is primarily symplastic. 相似文献
4.
Zhou Q Wang L Cai X Wang D Hua X Qu L Lin J Chen T 《Journal of plant physiology》2011,168(11):1249-1255
Casparian bands of endodermis and exodermis play crucial roles in blocking apoplastic movement of ions and water into the stele of roots through the cortex. These apoplastic barriers differ considerably in structure and function along the developing root. The present study assessed net Na+ fluxes in anatomically distinct root zones of rice seedlings and analyzed parts of individual roots showing different Na+ uptake. The results indicated that anatomically distinct root zones contributed differently to the overall uptake of Na+. The average Na+ uptake in root zones in which Casparian bands of the endo- and exo-dermis were interrupted by initiating lateral root primordia (root zone III) was significantly greater than that at the root apex, where Casparian bands were not yet formed (root zone I), or in the region where endo- and exo-dermis with Casparian bands were well developed (root zone II). The measurement of net Na+ fluxes using a non-invasive scanning ion-selective electrode technique (SIET) demonstrated that net Na+ flux varied significantly in different positions along developing rice roots, and a net Na+ influx was obvious at the base of young lateral root primordia. Since sodium fluxes changed significantly along developing roots of rice seedlings, we suggest that the significantly distinct net Na+ flux profile may be attributed to different apoplastic permeability due to lateral root primordia development for non-selective apoplastic bypass of ions along the apoplast. 相似文献
5.
菰(Zizania latifolia)是一种多年生挺水植物,为了探讨该植物根、茎和叶的解剖结构、组织化学及其质外体屏障的通透性生理。该文利用光学显微镜和荧光显微镜,对菰的根、茎、叶进行了解剖学和组织化学研究。结果表明:(1)菰不定根解剖结构由外而内分别为表皮、外皮层、单层细胞的厚壁机械组织层、皮层、内皮层和维管柱;茎结构由外而内分别为角质层、表皮、周缘厚壁机械组织层、皮层、具维管束的厚壁组织层和髓腔。叶鞘具有表皮和具维管束皮层,叶片具有表皮,叶肉和维管束。(2)不定根具有位于内侧的内皮层及其邻近栓质化细胞和外侧的外皮层组成的屏障结构;茎具内侧厚壁机械组织层,外侧的角质层和周缘厚壁机械组织层组成的屏障结构,屏障结构的细胞壁具凯氏带、木栓质和木质素沉积的组织化学特点,叶表面具有角质层。(3)菰通气组织包括根中通气组织,茎、叶皮层的通气组织和髓腔。(4)菰的屏障结构和解剖结构是其适应湿地环境的重要特征,但其茎周缘厚壁层和厚壁组织层较薄。由此推测,菰适应湿地环境,但在旱生环境中分布有一定的局限性。 相似文献
6.
Background and Aims
Most studies of exodermal structure and function have involved species with a uniseriate exodermis. To extend this work, the development and apoplastic permeability of Iris germanica roots with a multiseriate exodermis (MEX) were investigated. The effects of different growth conditions on MEX maturation were also tested. In addition, the exodermises of eight Iris species were observed to determine if their mature anatomy correlated with habitat.Methods
Plants were grown in soil, hydroponics (with and without a humid air gap) or aeroponics. Roots were sectioned and stained with various dyes to detect MEX development from the root apical meristem, Casparian bands, suberin lamellae and tertiary wall thickenings. Apoplastic permeability was tested using dye (berberine) and ionic (ferric) tracers.Key Results
The root apical meristem was open and MEX development non-uniform. In soil-grown roots, the exodermis started maturing (i.e. Casparian bands and suberin lamellae were deposited) 10 mm from the tip, and two layers had matured by 70 mm. In both hydro- and aeroponically grown roots, exodermal maturation was delayed. However, in areas of roots exposed to an air gap in the hydroponic system, MEX maturation was accelerated. In contrast, maturation of the endodermis was not influenced by the growth conditions. The mature MEX had an atypical Casparian band that was continuous around the root circumference. The MEX prevented the influx and efflux of berberine, but had variable resistance to ferric ions due to their toxic effects. Iris species living in well-drained soils developed a MEX, but species in water-saturated substrates had a uniseriate exodermis and aerenchyma.Conclusions
MEX maturation was influenced by the roots'' growth medium. The MEX matures very close to the root tip in soil, but much further from the tip in hydro- and aeroponic culture. The air gap accelerated maturation of the second exodermal layer. In Iris, the type of exodermis was correlated with natural habitat suggesting that a MEX may be advantageous for drought tolerance.Key words: Iris germanica, roots, culture conditions, development, anatomy, apoplastic tracers, multiseriate exodermis, endodermis, root apical meristem 相似文献7.
A hypodermal Casparian band develops 4050 mm from theroot tip in corn and 3040 mm from the root tip in onion.In both plants, the endodermal Casparian band matures about20 mm closer to the root tip than the hypodermal Casparian band.Using the apoplastic fluorescent dye, Calcofluor white M2R (CFW),a permeability barrier could be distinguished in the radialwalls of the hypodermis 4050 mm from the root tip incorn and onion. In progressively younger regions of the roots,CFW was first excluded from the outer tangential hypodermalwalls and the inner tangential epidermal walls, then the radialepidermal walls so that in very young regions only the outertangential epidermal walls were permeated. In contrast to CFW,the symplastic fluorescent dye, uranin, was translocated fromthe epidermis into the stele at all distances tested (5.050mm from the root tips). CFW and uranin at a concentration of0.01% proved nontoxic to corn and onion roots on the basis ofroot growth tests. Key words: Zea mays, Casparian band, Hypodermis, Allium cepa 相似文献
8.
Chemical composition of apoplastic transport barriers in relation to radial hydraulic conductivity of corn roots (Zea mays L.) 总被引:5,自引:0,他引:5
The hydraulic conductivity of roots (Lpr) of 6- to 8-d-old maize seedlings has been related to the chemical composition of apoplastic transport barriers in the endodermis
and hypodermis (exodermis), and to the hydraulic conductivity of root cortical cells. Roots were cultivated in two different
ways. When grown in aeroponic culture, they developed an exodermis (Casparian band in the hypodermal layer), which was missing
in roots from hydroponics. The development of Casparian bands and suberin lamellae was observed by staining with berberin-aniline-blue
and Sudan-III. The compositions of suberin and lignin were analyzed quantitatively and qualitatively after depolymerization
(BF3/methanol-transesterification, thioacidolysis) using gas chromatography/mass spectrometry. Root Lpr was measured using the root pressure probe, and the hydraulic conductivity of cortical cells (Lp) using the cell pressure
probe. Roots from the two cultivation methods differed significantly in (i) the Lpr evaluated from hydrostatic relaxations (factor of 1.5), and (ii) the amounts of lignin and aliphatic suberin in the hypodermal
layer of the apical root zone. Aliphatic suberin is thought to be the major reason for the hydrophobic properties of apoplastic
barriers and for their relatively low permeability to water. No differences were found in the amounts of suberin in the hypodermal
layers of basal root zones and in the endodermal layer. In order to verify that changes in root Lpr were not caused by changes in hydraulic conductivity at the membrane level, cell Lp was measured as well. No differences
were found in the Lp values of cells from roots cultivated by the two different methods. It was concluded that changes in
the hydraulic conductivity of the apoplastic rather than of the cell-to-cell path were causing the observed changes in root
Lpr.
Received: 17 March 1999 / Accepted: 22 June 1999 相似文献
9.
黄连(Coptis chinensis)是毛茛科著名药材,该文研究了黄连体内黄连素在组织器官中的分布规律和根尖屏障结构特征。在白光和荧光显微镜下,组织器官中黄连素在蓝色激发光下自发黄色荧光,黄连素-苯胺兰对染研究细胞壁凯氏带和木质化,苏丹7B染色栓质层,间苯三酚-盐酸染色木质化。结果表明:黄连不定根初生结构为维管柱、内皮层、皮层、外皮层和表皮组成;次生结构以次生木质部为主、次生韧皮部和木栓层组成。黄连根茎初生结构由角质层,皮层和维管柱组成;次生结构由木栓层、皮层和维管柱组成,以皮层和维管柱为主。叶柄结构为髓、含维管束的厚壁组织层、皮层和角质层。黄连不定根的屏障结构初生结构时期由栓质化和木质化的内皮层、外皮层;次生结构时期为木栓层组成;根状茎的为角质层和木栓层。黄连素主要沉积分布在不定根和茎的木质部,叶柄的厚壁组织层,木质部和厚壁组织是鉴别黄连品质的重要部位。黄连根尖外皮层及早发育,同时初生木质部有黄连素沉积结合,可能造成水和矿质吸收和运输的阻碍,也是黄连适应阴生环境的重要原因。 相似文献
10.
Growing tree roots are characteristically brown with white tips. The browning process, which occurs as the white region matures, has often been attributed to the deposition of suberin in various tissues. However, in pouch-grown tree seedlings of jack pine (Pinus banksiana Lamb.) and eucalyptus (Eucalyptus pilularis Sm.), browning was not linked to suberization but was caused by the deposition of condensed tannins in the walls of all cells external to the stele. Therefore, we propose using the term “tannin zone” to refer to this region of the root. Vitality tests indicated that the cells of the epidermis and cortex were alive in white regions but were dead in brown regions. Following sequential treatment with berberine hemisulfate and potassium thiocyanate, the cortical walls external to the endodermal Casparian band were full of berberine thiocyanate crystals, indicating that they were permeable to berberine. These walls should also be permeable to water and ions, which have smaller molecular dimensions than the tracer dye. Based on the anatomy and permeability of the tannin zone, we predict that its capacity for ion uptake would be reduced compared to the white zone because of a reduced absorptive plasmalemma surface area. In jack pine, some uptake could be effected by the passage cells of the endodermis. The tannin zone should be even less absorptive in eucalyptus because the exodermis remains an apoplastic barrier and the endodermis lacks passage cells. It is difficult to predict the difference between the tannin and white zones with respect to water uptake. Death of the cells external to the endodermis would reduce the resistance of the root to water movement, but deposition of tannins would increase it. The deposition of suberin lamellae in increasing numbers of endodermal cells may also retard water flow. The anatomy and physiological properties of the tannin zone are unique from those of the distal, white zone and the proximal, cork-clad zone. 相似文献
11.
The paper investigates how the apoplastic route of ion transfer is affected by the outermost cortex cell layers of a primary
root. Staining of hand-made cross sections with aniline blue in combination with berberine sulfate demonstrated the presence
of casparian bands in the endo- and exodermis, potentially being responsible for hindering apoplastic ion movement. The use
of the apoplastic dye Evan's Blue allowed viewing under a light microscope of potential sites of uncontrolled solute entry
into the apoplast of the root cortex which mainly consisted of injured rhizodermis and/or exodermis cells. The distribution
of the dye after staining was highly comparable to EDX analyses on freeze-dried cryosectioned roots. Here, we used Rb+ as
a tracer for K+ in a short-time application on selected regions of intact roots from intact plants. After subsequent quench-freezing
with liquid propane the distribution of K+ and Rb+ in cell walls was detected on freeze-dried cryosections by their specific
X-rays resulting from the incident electrons in a SEM. All such attempts led to a single conclusion, namely, that the walls
of the two outermost living cell sheaths of the cortex largely restrict passive solute movements into the apoplast. The ring
of turgescent living rhizodermis cells in the root tip region forms the first barrier. With increasing distance to the root
tip, in the course of their maturation resp. degradation, this particular function of the rhizodermis cells is replaced by
the hypodermis resp. exodermis. Furthermore, the restriction of apoplastic ion flow by the outermost cortex cell layers is
rather effective but not complete. Thus, the solute transfer into the stele is mainly restricted by the casparian bands of
the endodermis. The overall conclusion is that the resistances of the rhizodermis and exodermis are additive to the endodermis
in their role of regulating the apoplastic solute movement across roots.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
12.
Development and Structure of the Root Cortex in Caltha palustris L. and Nymphaea odorata Ait. 总被引:1,自引:0,他引:1
Seago James L. Jr; Peterson Carol A.; Kinsley Laura J.; Broderick Jennifer 《Annals of botany》2000,86(3):631-640
Structural features of the mature root cortex and its apoplasticpermeability to dyes have been determined for two dicotyledonouswetland plants of differing habitats: Nymphaea odorata, growingrooted in water and mud, and Caltha palustris, growing in temporalwetlands among cattails. In mature roots, movement of the apoplasticdyes, berberine and safranin, into the roots was blocked atthe hypodermis, indicating the presence of an exodermis. A hypodermiswith an exodermis, i.e. Casparian bands in the outermost uniseriatelayer plus suberin lamellae, is present in both species. InN. odorata, hypodermal walls are further modified with cellulosicsecondary walls. Roots of N. odorata and C. palustris have anendodermis with Casparian bands only. A honeycomb aerenchymais produced by differential expansion in N. odorata and includesastrosclereids and diaphragms, while roots of C. palustris haveno aerenchyma, but some irregular lacunae are found in old roots.These aspects of cortex structure are related to an open meristemorganization, with unusual patterns of cell divisions in certainground meristem cells (called semi-regular hexagon cells) ofN. odorata. The correlation between aerenchyma pattern and hypodermalstructure appears to be related to habitat differences.Copyright2000 Annals of Botany Company Caltha palustris, Nymphaea odorata, root development, cortex, endodermis, aerenchyma, exodermis, hypodermis, permeability, wetland plants 相似文献
13.
M. Damus R. L. Peterson Daryl E. Enstone Carol A. Peterson 《Plant biology (Stuttgart, Germany)》1997,110(2):190-195
Forty-three species of seedless vascular plants were assessed for modifications to root cortical cell walls. All species except Lycopodium had an endodermis with distinct Casparian bands. Experiments with the apoplastic tracer berberine hemisulfate showed that walls of all root cortical cells in the two Lycopodium species tested were permeable to this tracer. Although most species examined lacked a hypodermis several Equisetum species had a hypodermis with modified walls. Three Selaginella species had distinct Casparian bands in this cortical cell layer. This layer, therefore, is an exodermis in Selaginella and its presence limited the inward diffusion of the apoplastic tracer berberine hemisulfate. 相似文献
14.
The exodermis: a variable apoplastic barrier. 总被引:29,自引:0,他引:29
E Hose D T Clarkson E Steudle L Schreiber W Hartung 《Journal of experimental botany》2001,52(365):2245-2264
The exodermis (hypodermis with Casparian bands) of plant roots represents a barrier of variable resistance to the radial flow of both water and solutes and may contribute substantially to the overall resistance. The variability is a result largely of changes in structure and anatomy of developing roots. The extent and rate at which apoplastic exodermal barriers (Casparian bands and suberin lamellae) are laid down in radial transverse and tangential walls depends on the response to conditions in a given habitat such as drought, anoxia, salinity, heavy metal or nutrient stresses. As Casparian bands and suberin lamellae form in the exodermis, the permeability to water and solutes is differentially reduced. Apoplastic barriers do not function in an all-or-none fashion. Rather, they exhibit a selectivity pattern which is useful for the plant and provides an adaptive mechanism under given circumstances. This is demonstrated for the apoplastic passage of water which appears to have an unusually high mobility, ions, the apoplastic tracer PTS, and the stress hormone ABA. Results of permeation properties of apoplastic barriers are related to their chemical composition. Depending on the growth regime (e.g. stresses applied) barriers contain aliphatic and aromatic suberin and lignin in different amounts and proportion. It is concluded that, by regulating the extent of apoplastic barriers and their chemical composition, plants can effectively regulate the uptake or loss of water and solutes. Compared with the uptake by root membranes (symplastic and transcellular pathways), which is under metabolic control, this appears to be an additional or compensatory strategy of plants to acquire water and solutes. 相似文献
15.
Blockage of apoplastic bypass-flow of water in rice roots by insoluble salt precipitates analogous to a Pfeffer cell 总被引:5,自引:1,他引:4
Precipitates of insoluble inorganic salts were used to clog apoplastic pores in cell walls of the outer part of rice roots (OPR) in two rice cultivars (lowland cv. IR64 and upland cv. Azucena). Aerenchyma of two different root zones (20–50 and 50–100 mm from the apex) was perfused with 1 m m potassium ferrocyanide (K4[Fe(CN)6]) while the whole root segments were bathed in 0.5 m m copper sulphate (CuSO4) medium. In another experiment, salts were applied on opposite sides of the OPR. The copper-ferrocyanide precipitation technique resembles the famous osmotic experiments of the German botanist Wilhelm Pfeffer, in which he used them with clay diaphragms. Precipitates were observed on the side where ferrocyanide was applied, suggesting that Cu2+ and SO42– were passing the barrier including the Casparian bands of the exodermis much faster than ferrocyanide. There was a patchiness in the formation of precipitates, correlated with the maturation of the exodermis. The intensity of copper ferrocyanide staining decreased along developing rice roots. No precipitates were observed in mature parts beyond 70–80 mm from the root apex, except for sites around the emergence of secondary roots, which were fairly leaky to both water and ions. Blockage of the apoplastic pores with precipitates caused a three- to four-fold reduction of hydraulic conductivity of the OPR (LpOPR). The reflection coefficient of the OPR (σsOPR) increased in response to the blockage with precipitates. The osmotic versus diffusive water permeability ratios of the OPR (PfOPR/PdOPR) were around 600 for immature and 1200 for mature root segments. Treatment significantly affected the bulk rather than the diffusive water flow and caused a three- to five-fold reduction of the PfOPR/PdOPR ratios. Results indicated that despite the existence of an exodermis with Casparian bands, most of the water moved around cells rather than using the cell-to-cell passage. 相似文献
16.
Krishnamurthy P Ranathunge K Nayak S Schreiber L Mathew MK 《Journal of experimental botany》2011,62(12):4215-4228
Rice is an important crop that is very sensitive to salinity. However, some varieties differ greatly in this feature, making investigations of salinity tolerance mechanisms possible. The cultivar Pokkali is salinity tolerant and is known to have more extensive hydrophobic barriers in its roots than does IR20, a more sensitive cultivar. These barriers located in the root endodermis and exodermis prevent the direct entry of external fluid into the stele. However, it is known that in the case of rice, these barriers are bypassed by most of the Na(+) that enters the shoot. Exposing plants to a moderate stress of 100 mM NaCl resulted in deposition of additional hydrophobic aliphatic suberin in both cultivars. The present study demonstrated that Pokkali roots have a lower permeability to water (measured using a pressure chamber) than those of IR20. Conditioning plants with 100 mM NaCl effectively reduced Na(+) accumulation in the shoot and improved survival of the plants when they were subsequently subjected to a lethal stress of 200 mM NaCl. The Na(+) accumulated during the conditioning period was rapidly released when the plants were returned to the control medium. It has been suggested that the location of the bypass flow is around young lateral roots, the early development of which disrupts the continuity of the endodermal and exodermal Casparian bands. However, in the present study, the observed increase in lateral root densities during stress in both cultivars did not correlate with bypass flow. Overall the data suggest that in rice roots Na(+) bypass flow is reduced by the deposition of apoplastic barriers, leading to improved plant survival under salt stress. 相似文献
17.
Apoplastic transport across young maize roots: effect of the exodermis 总被引:27,自引:0,他引:27
The uptake of water and of the fluorescent apoplastic dye PTS (trisodium 3-hydroxy-5,8,10-pyrenetrisulfonate) by root systems
of young maize (Zea mays L.) seedlings (age: 11–21 d) has been studied with plants which either developed an exodermis (Casparian band in the hypodermis)
or were lacking it. Steady-state techniques were used to measure water uptake across excised roots. Either hydrostatic or
osmotic pressure gradients were applied to induce water flows. Roots without an exodermis were obtained from plants grown
in hydroponic culture. Roots which developed an exodermis were obtained using an aeroponic (=mist) cultivation method. When
the osmotic concentration of the medium was varied, the hydraulic conductivity of the root (Lp
r in m3 · m−2 · MPa−1 · s−1) depended on the osmotic pressure gradient applied between root xylem and medium. Increasing the gradient (i.e. decreasing
the osmotic concentration of the medium; range: zero to 40 mM of mannitol), increased the osmotic Lp
r. In the presence of hydrostatic pressure gradients applied by a pressure chamber, root Lp
r was constant over the entire range of pressures (0–0.4 MPa). The presence of an exodermis reduced root Lp
r in hydrostatic experiments by a factor of 3.6. When the osmotic pressure of the medium was low (i.e. in the presence of a
strong osmotic gradient between xylem sap and medium), the presence of an exodermis caused the same reduction of root Lp
r in osmotic experiments as in hydrostatic ones. However, when the osmotic concentration of the medium was increased (i.e.
the presence of low gradients of osmotic pressure), no marked effect of growth conditions on osmotic root Lp
r was found. Under these conditions, the absolute value of osmotic root Lp
r was lower by factors of 22 (hydroponic culture) and 9.7 (aeroponic culture) than in the corresponding experiments at low
osmotic concentration. Apoplastic flow of PTS was low. In hydrostatic experiments, xylem exudate contained only 0.3% of the
PTS concentration of the bathing medium. In the presence of osmotic pressure gradients, the apoplastic flow of PTS was further
reduced by one order of magnitude. In both types of experiments, the development of an exodermis did not affect PTS flow.
In osmotic experiments, the effect of the absolute value of the driving force cannot be explained in terms of a simple dilution
effect (Fiscus model). The results indicate that the radial apoplastic flows of water and PTS across the root were affected
differently by apoplastic barriers (Casparian bands) in the exodermis. It is concluded that, unlike water, the apoplastic
flow of PTS is rate-limited at the endodermis rather than at the exodermis. The use of PTS as a tracer for apoplastic water
should be abandoned.
Received: 9 October 1997 / Accepted: 5 February 1998 相似文献
18.
The dimorphic exodermis of the root of onion (Allium cepa L.) consists of long and short cells, both of which have Casparian bands. The long cells and some of the short cells also have suberin lamellae. The proportion of short cells with lamellae increases with distance from the root tip and with plant age, but is not influenced by drought stress. In young regions of onion roots, characterized by a mature endodermis and an immature exodermis, the plasmalemma surface area that can be contacted by the soil solution is 90·9 mm2 per mm length of root, i.e. the sum of the plasmalemma surface areas of the epidermis, immature exodermis, cortical parenchyma and endodermis external to the Casparian band. This is reduced to 14·5–14·7 mm2 by the development of a Casparian band in the exodermis, which cuts off access to the cortical parenchyma, and by the development of suberin lamellae, which cut off access to the plasmalemmae of the long and some of the short cells of the exodermis. Death of all the epidermal cells, a consequence of drought, further reduces this area to 0·205–0·0183 mm2, i.e. the area of the outer tangential plasmalemmae of the short cells without suberin lamellae. In this condition, the root's capacity for ion uptake should be reduced but its capacity to resist water loss to the soil should be increased. 相似文献
19.
The periderm in roots of Pinus banksiana Lamb. and the polyderm in roots of Eucalyptus pilularis Sm. originate from the pericycle. This occurs after the roots have turned brown due to deposition of tannins in the walls of cells external to the endodermis. In both species, cork cells form a continuous sheath around the vascular tissues. The cork cell walls are modified by the presence of suberin, lignin and tannin and it is the latter which imparts a brown colour to the tissue. The first layer of cork cells in both species constitutes an apoplastic barrier which prevents the fluorescent dye, berberine, from entering the vascular tissues, despite the absence of an identifiable Casparian band in the cells. Because the roots are still covered with the cortex and epidermis during early stages of periderm and polyderm formation, it is not possible to tell from the external aspect of the root when it makes a transition from the tannin zone to the cork zone. 相似文献
20.
The root system is particularly affected by unfavourable conditions because it is in direct contact with the soil environment. Casparian strips, a specialised structure deposited in anticlinal walls, are characterised by the impregnation of the primary wall pores with lignin and suberin. The Casparian strips in the endo- and exodermis of vascular plant roots appear to play an important role in preventing the non-selective apoplastic bypass of salts into the stele along the apoplast under salt stress. However, only a few investigations have examined the deposition and function of these apoplastic barriers in response to salt stress in higher plants. 相似文献