268例难治性下呼吸道感染的细菌学分析

通过本组２６８ 例难治性呼吸道感染病人标本培养结果分析,阳性２４６ 例占９１．８ ％ ,有１５４ 例检出厌氧菌,占５７ ％ ,１０４ 例检出需氧菌,占３９ ％ ,混合感染１３３ 例,占５０ ％ 。试验结果证实,厌氧菌感染在难治性下呼吸道感染病例中占了很大比例,这一结果为难治性下呼吸道感染的治疗提供了有价值的参考数据。     

Time-Dependent Effects of Inosine on Competence Development in Haemophilus influenzae

The effect of inosine on the development of competence is dependent upon the time at which it is added to the competence medium. Competence is inhibited if inosine is added within 15 min after the development begins, but is stimulated if the addition is made at later times. Protein synthesis is stimulated by the addition of inosine, but the effect is quantitatively similar regardless of the time at which inosine is added.     

Influx of γ-Aminobutyric Acid and α-Aminoisobutyric Acid into Mouse Cerebrum Slices Incubated in a Pyruvate Medium with or without Added Glucose or Glucose Analogues Compared with Influx from a Glucose Medium

Concentrative influx of γ-aminobutyric acid (GABA) and α-aminoisobutyric acid (AIB) into incubated mouse cerebrum slices is decreased when pyruvate is substituted for glucose. Influx of GABA from pyruvate medium is not increased by presence of glucose, 2-deoxy-d -glucose (2-DOG), or 3-O-methyl-d -glucose (3-O-MeG). Influx of AIB is restored to the rate from glucose medium if 2-DOG is present initially, but is not restored if 2-DOG is added with AIB. Influx is not restored if 3-O-MeG is present initially, but is restored if 3-O-MeG is added with AIB. Influx is restored if glucose is present initially or is added with AIB.     

药敏药片对临床235株细菌实验观察报告

药敏药片经临床对金黄色葡萄球菌、大肠埃希氏菌、铜绿假单胞菌等２３５株考核．表明药片工艺研究先进,药片与培养基结合牢固,无断裂、崩解,不渗出颗粒,抑菌圈呈同心园扩散．边缘清楚。药物含量均匀,释放度好。药片抑菌差仅１～３ｍｍ;而纸片抑菌差为２～１２ｍｍ。药片变黑系数ＣＶ为２．７１～４．２１;而纸片ＣＶ为３．８２～１４．３６。表明纸片片间差大,药片精密度明显好于纸片。     

Backward bifurcation of an epidemic model with treatment

An epidemic model with a limited resource for treatment is proposed to understand the effect of the capacity for treatment. It is assumed that treatment rate is proportional to the number of infectives below the capacity and is a constant when the number of infectives is greater than the capacity. It is found that a backward bifurcation occurs if the capacity is small. It is also found that there exist bistable endemic equilibria if the capacity is low.     

Autogenous control of Escherichia coli threonyl-tRNA synthetase expression in vivo

The regulation of the expression of thrS, the structural gene for threonyl-tRNA synthetase, was studied using several thrS-lac fusions cloned in lambda and integrated as single copies at att lambda. It is first shown that the level of beta-galactosidase synthesized from a thrS-lac protein fusion is increased when the chromosomal copy of thrS is mutated. It is also shown that the level of beta-galactosidase synthesized from the same protein fusion is decreased if wild-type threonyl-tRNA synthetase is overproduced from a thrS-carrying plasmid. These results strongly indicate that threonyl-tRNA synthetase controls the expression of its own gene. Consistent with this hypothesis it is shown that some thrS mutants overproduce a modified form of threonyl-tRNA synthetase. When the thrS-lac protein fusion is replaced by several types of thrS-lac operon fusions no effect of the chromosomal thrS allele on beta-galactosidase synthesis is observed. It is also shown that beta-galactosidase synthesis from a promoter-proximal thrS-lac operon fusion is not repressed by threonyl-tRNA synthetase overproduction. The fact that regulation is seen with a thrS-lac protein fusion and not with operon fusions indicates that thrS expression is autoregulated at the translational level. This is confirmed by hybridization experiments which show that under conditions where beta-galactosidase synthesis from a thrS-lac protein fusion is derepressed three- to fivefold, lac messenger RNA is only slightly increased.     

退休教师骨密度的超声研究

目的：了解老年教师骨质疏松病的情况,用超声骨密度仪对813例老年教师做跟骨超声振幅衰减值研究。方法：超声测量是一种新技术,利用不同密度的物体衰减值不同及穿过不同密度物体声速不同等特性对跟骨进行成像,并计算最具说明性区域的一系列数据。参数中BUA值为最主要的参数,三根等距离曲线的形态和宽度能直观分析出年龄与骨量变化的关系。结果：本文男性平均BUA为63.87dB/MHz,得出回归公式：体重=189.1417 0.2062BUA-0.0224SOS。女性平均BUA为58.9dB/MHz,各年龄分组的BUA值与参考值很接近,统计学显示无意义。结论：调查发现男性和女性的骨质不同,男性BUA骨峰值比女性高。BUA值与其体重有密切关系。在60岁以后（或绝经1-5年后）年龄增长与BUA值无明显关系,说明随着年龄的增加骨量减少很小或不减少。利用这种关系,假如BUA值突然下降则提示近期发生骨质疏松。     

杉木人工林水量平衡和蒸散的研究

 本文根据我们严密设计的小集水区径流场连续6年的水文测定数据,进行了杉木人工林水量平衡和蒸散的研究。结果表明：集水区年平均降雨量1065.5mm, 在林冠作用面降雨量的分配中,林冠截留雨量264.6mm,截留率24.8%;穿透过林冠层的雨量799.82mm,树干径流量1.08mm,分别占降雨量的75.1%和0.1%。林内降水到达林地时,在枯枝落叶层这个作用面上净降水进行再分配,其中,地表径流量9.27mm,地下径流量203.00mm,总径流系数0.199。土壤蓄水量月变化较大,但年变化很小,占降雨量的1.2%。系统水量最大的输出是蒸散,每年以气态形式返回大气的水量866.03mm,占降雨量81.3%。在蒸散的水量中,林冠截留雨量的直接物理蒸发量为264.6mm,占总蒸散量的31.6%。     

Colcemid-resistant mutants of fission yeast have an altered cell cycle

Cell-cycle progression is altered in some colcemid-resistant mutants of fission yeast. The duration of particular stages of the cell cycle is different but total doubling time is unchanged from that of wild type. Cell-plate formation is prolonged relative to wild type but concomitant DNA synthesis is not affected. Separation of daughter cells is inhibited in some strains, giving rise to unusual cell morphologies. Control of cell division is altered in two ways: Critical size for division is increased. The probability of division a function of size is decreased.     

两型豆花粉和叶表皮的扫描电镜观察

两型豆花粉和叶表皮在扫描电镜下的形态特征：花粉近球形至矩球形,三孔沟,外壁表面较粗糙,侧壁具疣状突起至块状突起,极面具粗网状纹饰,但两型豆祖山居群属于萌发沟不明显型,两型豆燕塞湖居群属于萌发沟明显型。叶表皮细胞不规则,排列紧密,叶脉表皮细胞长圆柱形,长轴与叶脉平行,角质膜薄,表皮毛均为单细胞非腺毛,气孔器仅分布在下表皮,属于平列型,偶见不规则型。表皮毛和气孔器在叶片上分布不均匀,两型豆祖山居群叶表皮毛和气孔器的平均密度大于两型豆燕塞湖居群叶表皮毛和气孔器的平均密度,自然生长的两型豆叶表皮毛和气孔器的平均密度大于遮光条件下的两型豆。     

Ultrastructural organization of the ocelloide of Nematodinium. Phylogenetic aspect of the evolution of Warnowiidae Lindemann dinoflagellates photoreceptor (author's transl)

In this study, more details of the different parts of the "ocelloide" of Nematodinium are given. The "hyalosome" which has a radial symmetry is made of endoplasmic reticulum, except the mitochondrial layer. There is only one constrictor ring and only a beginning of the periocellar gallery. The basal sheet on which the crystalline lens lies is a local differentiation of the cortical layer which is observed on the ceiling of the ocellar camera. This camera is related to the intercingular sulcus. There is also an ocellar fiber. The "melanosome" which is made of a double membrane and of many couples of thylakoids is flattened dorso-ventrally. The structure of the retina is similar to that observed in the other Warnowiidae. The pigmented ring is rather well developed and is in continuity with the upper part of the retina. Owing to the simpler organization of its ocelloide, Nematodinium is situated before the genera Warnowia and Erythropsidinium in the evolutionary series.     

Phylogenetic relationships among superfamilies of Hymenoptera

The first comprehensive analysis of higher‐level phylogeny of the order Hymenoptera is presented. The analysis includes representatives of all extant superfamilies, scored for 392 morphological characters, and sequence data for four loci (18S, 28S, COI and EF‐1α). Including three outgroup taxa, 111 terminals were analyzed. Relationships within symphytans (sawflies) and Apocrita are mostly resolved. Well supported relationships include: Xyeloidea is monophyletic, Cephoidea is the sister group of Siricoidea + [Xiphydrioidea + (Orussoidea + Apocrita)]; Anaxyelidae is included in the Siricoidea, and together they are the sister group of Xiphydrioidea + (Orussoidea + Apocrita); Orussoidea is the sister group of Apocrita, Apocrita is monophyletic; Evanioidea is monophyletic; Aculeata is the sister group of Evanioidea; Proctotrupomorpha is monophyletic; Ichneumonoidea is the sister group of Proctotrupomorpha; Platygastroidea is sister group to Cynipoidea, and together they are sister group to the remaining Proctotrupomorpha; Proctotrupoidea s. str. is monophyletic; Mymarommatoidea is the sister group of Chalcidoidea; Mymarommatoidea + Chalcidoidea + Diaprioidea is monophyletic. Weakly supported relationships include: Stephanoidea is the sister group of the remaining Apocrita; Diaprioidea is monophyletic; Ceraphronoidea is the sister group of Megalyroidea, which together form the sister group of [Trigonaloidea (Aculeata + Evanioidea)]. Aside from paraphyly of Vespoidea within Aculeata, all currently recognized superfamilies are supported as monophyletic. The diapriid subfamily Ismarinae is raised to family status, Ismaridae stat. nov. © The Will Henning Society 2011.     

Discrete three-stage population model: persistence and global stability results

A general three-stage discrete-time population model is studied. The inherent net reproductive number for this model is derived. Global stability of the origin is established provided that the inherent net reproductive number is less than one. If it is larger than one the existence of a unique positive fixed point is proved and the persistence of the system is established. Finally, for certain parameter ranges global stability of the positive fixed point is proved.     

Homology and function in the wings of Heteroptera

Abstract. The homology of veins and other wing characters in Heteroptera is reviewed in the light of palaeontology and new functional studies. A cladogram is given for the higher taxa of Hemiptera. It is probable that the vannus is an autapomorphy of Auchenorrhyncha+Heteropteroidea; that the leading edge vein of heteropteran fore- and hindwings is C+Sc; that Rs cannot be distinguished from R; that the hamus is part of M; that the glochis is a secondary structure. The difficulty of defining a vein is stressed. The functional significance of the hemielytron, cuneal fracture and longitudinal flexion lines is discussed. A preliminary ground-plan for Heteroptera wings is presented.     

Antidiuretic mechanism in Rhodnius prolixus (Stal, 1859) (Hemiptera, Reduviidae)

An antidiuretic mechanism is proposed for Rhodnius prolixus, whose activity is manifested when the diuretic phase has terminated and is maintained until the insect has fed again. Presumably this mechanism acts at the level of the proximal rectal sphincter and is inhibited by ingestion, mechanical distension of the gut and central disinhibition by decapitation. It is suggested that the antidiuretic activity is maintained by the nervous system and is modified when there is distention of the gut or when the nervous signal is interrupted by decapitation. It is demonstrated that the excretion of urine in R. prolixus is not controlled exclusively by the diuretic hormone, but rather that factors not linked to the haemolymph maintain the insects in a diuretic or nondiuretic state.     

Control of glucose metabolism in the perinatal period

The central importance of glucose as a fuel for energy metabolism and growth of the fetus is clear as is the role of insulin in coordinating its utilisation by many fetal tissues. What is less clear is the qunatitative nature of the interaction between the fetus and placenta in organising glucose metabolism. Increasingly there is evidence that the fetus coordinates some of the supply of glucose to the placenta and that this is particularly important when uterine blood flow is reduced. It is unclear how this is regulated, but substrate cycles of glucose and lactate appear to make a significant contribution to carbohydrate metabolism in fetus and placenta. Another area as yet unresolved in the control of fetal glucose metabolism is the coordination of the changes that occur around the time of birth. Notable of these is the activation of glycogen mobilisation and of glucose synthesis and changes in the setting of glucose regulatory mechanism. These are briefly reviewed.     

药物敏感试验药片法研究

药敏药片对国际三株标准菌试验,抑菌圈直径范围均符合判定标准。与进口药敏药片符合率为１００％。与药敏纸片片间差比较试验,药片抑菌圈＝１～２ｍｍ,精密度高,符合国际要求,而纸片抑菌圈＝９～１３ｍｍ,均匀度检测明显不合格。药片变异系数ＣＶ＝１．３１～２．９２,纸片ＣＶ＝１４．７８～２１．６７表明药片变量离散度小,片间差很小;而纸片变量离散度明显大,故纸片片间差很大。     

Discrete three-stage population model: persistence and global stability results

A general three-stage discrete-time population model is studied. The inherent net reproductive number for this model is derived. Global stability of the origin is established provided that the inherent net reproductive number is less than one. If it is larger than one the existence of a unique positive fixed point is proved and the persistence of the system is established. Finally, for certain parameter ranges global stability of the positive fixed point is proved.     

黑鸢消化系统形态学的初步研究

对黑鸢消化系统的形态学作了初步的观察与研究,结果表明：黑鸢舌尖钝圆,舌根分叉,叉粘膜上具有尖端指向后方的栉状突;食管宽而扁,无明显可见的嗉囊;肌胃无明显的类角质膜;肠道长是体长2．3倍,小肠较发达,K1275mm,古肠道总长的93．6％,具有不发达的双侧盲肠：胰腺短而厚、并与蛇雌、毛脚鸢的消化系统进行了比较。     

Equipoise, knowledge and ethics in clinical research and practice

It is widely maintained that a clinical trial is ethical only if some form of equipoise between the treatments being compared obtains. To be in equipoise between two treatments A and B is to be cognitively indifferent between the statement 'A is strictly more effective than B' and its negation. It is natural to claim that equipoise regarding A and B is necessary for randomised assignment to treatments A and B to be beneficent and non-maleficent and is sufficient for such an assignment to be fair. Cashing this out precisely is difficult, and various forms of equipoise have been discussed which consider whose equipoise is relevant to the decision. This is to make judgement of equipoise something to be managed socially, while its prima facie significance is supposedly cognitive. Recent reconstructions of equipoise-like concepts in epistemology give clues about how to understand equipoise cognitively. In this paper I examine some of this work and discuss how successful it has been. I suggest that while this work is promising, it still has far to go, and that while equipoise remains the best theory we have of the cognitive justification for clinical trials, it is nonetheless incoherent.