Conserving the Sacred Medicine Mountains: A Vegetation Analysis of Tibetan Sacred Sites in Northwest Yunnan

Mount Kawa Karpo of the Menri ('Medicine Mountains' in Tibetan), in the eastern Himalayas, is one of the most sacred mountains to Tibetan Buddhists. Numerous sacred sites are found between 1900 and 4000 m, and at higher elevations the area as a whole is considered a sacred landscape. Religious beliefs may affect the ecology of these sacred areas, resulting in unique ecological characteristics of importance to conservation; recent studies have demonstrated that sacred areas can often play a major role in conservation. The goal of this study is to preliminarily analyze the vegetation of sacred areas in the Menri region using existing vegetation maps and a Geographical Information System (GIS) for remote assessment. Sacred sites are compared to random points in the landscape, in terms of: elevation, vegetation, and nearness to villages; species composition, diversity, and richness; and frequency of useful and endemic plant species. Detrended correspondence analysis (DCA) ordination reveals that sacred sites differ significantly in both useful species composition (p=0.034) and endemic species composition (p=0.045). Sacred sites are located at lower elevations, and closer to villages, than randomly selected, non-sacred sites (p< 0.0001), and have higher overall species richness (p=0.033) and diversity (p=0.042). In addition, the high-elevation (> 4000 m) areas of the mountain - a sacred landscape - are found to have significantly more endemics than low-elevation areas (p<0.0001). These findings represent an initial analysis of sacred sites and suggest that sacred sites in the Menri region may be ecologically and ethnobotanically unique.     

Tibetan sacred sites conserve old growth trees and cover in the eastern Himalayas

Khawa Karpo, in the eastern Himalayas, is a mountain considered sacred throughout Tibet, and is internationally recognized as a global biodiversity hotspot. Numerous areas within this landscape are considered ‘sacred’ by the indigenous Tibetans of the region, who interact with these sites in ways potentially beneficial to conservation. Our previous remote sensing study indicated that sacred sites are found in habitats with greater species richness, diversity, and endemism than randomly selected non-sacred sites. This study examines the role of sanctity in biodiversity conservation within habitats in the Khawa Karpo region by pairing plots within the same habitats in sacred and non-sacred areas. Understory richness, diversity, cover, and number of useful species are measured; for trees, richness, diversity, cover, and density are measured. Results indicate that within habitats sanctity does not affect understory plant communities; however, within sacred areas trees are larger (p = 0.003) and forests have greater cover (p = 0.003) than non-sacred areas. Our results indicate that, whereas placement of sacred areas and preservation of vegetation cover affects useful plants, biodiversity and endemism, within habitats sacred sites preserve old growth trees and forest structure. In sum, Tibetan sacred sites are ecologically unique and important for conservation on varying scales of landscape, community, and species.     

The Impacts of Global and National Policy on the Management and Conservation of Sacred Groves of India

India is home to thousands of community-protected forests, called sacred groves. Sacred forests or groves are sites that have cultural or spiritual significance to the people who live around them. These areas may also be key reservoirs of biodiversity. In India, most sacred groves are managed by a community group, not by a government agency. They are often private or community land, not formal protected areas or parks. This poses an interesting challenge in terms of future management and possible policy relating to the sacred groves. On the international level, organizations such as the International Union for the Conservation of Nature and UNESCO have created guidelines for management of sacred sites. On the national level, India’s past Forest Acts and recent Forest Rights Act have relevance to the sacred groves. Local differences in land tenure also affect the groves. Ethnographic research conducted in 2009 and 2010 in the states of Meghalaya and Karnataka, India, evaluated the historic and current management and beliefs associated with sacred forests. Cultural change and pressure to use natural resources within the groves is leading to reduction of these forest areas. In the future, a creative combination of policy approaches to conserve groves that respects their spiritual values is recommended.     

Sonerila janakiana sp. nov., a stoloniferous species of Melastomataceae from India

Sonerila janakiana, a new tuberous, scapigerous and stoloniferous species of Melastomataceae collected from exposed wet rocks of evergreen and semi evergreen forests in the Nilgiris and Wayanad districts of Tamil Nadu and Kerala states on the Western Ghats in India is described and illustrated. Apart from other characteristics, it is distinguished from all hitherto known species of the genus in India by having stolons and dimorphic leaves. The stolons produced from tubers terminate and develop into new tubers producing new plants.     

Climatic controls of vegetation vigor in four contrasting forest types of India—evaluation from National Oceanic and Atmospheric Administration’s Advanced Very High Resolution Radiometer datasets (1990–2000)

Ten-day advanced very high resolution radiometer images from 1990 to 2000 were used to examine spatial patterns in the normalized difference vegetation index (NDVI) and their relationships with climatic variables for four contrasting forest types in India. The NDVI signal has been extracted from homogeneous vegetation patches and has been found to be distinct for deciduous and evergreen forest types, although the mixed-deciduous signal was close to the deciduous ones. To examine the decadal response of the satellite-measured vegetation phenology to climate variability, seven different NDVI metrics were calculated using the 11-year NDVI data. Results suggested strong spatial variability in forest NDVI metrics. Among the forest types studied, wet evergreen forests of north-east India had highest mean NDVI (0.692) followed by evergreen forests of the Western Ghats (0.529), mixed deciduous forests (0.519) and finally dry deciduous forests (0.421). The sum of NDVI (SNDVI) and the time-integrated NDVI followed a similar pattern, although the values for mixed deciduous forests were closer to those for evergreen forests of the Western Ghats. Dry deciduous forests had higher values of inter-annual range (RNDVI) and low mean NDVI, also coinciding with a high SD and thus a high coefficient of variation (CV) in NDVI (CVNDVI). SNDVI has been found to be high for wet evergreen forests of north-east India, followed by evergreen forests of the Western Ghats, mixed deciduous forests and dry deciduous forests. Further, the maximum NDVI values of wet evergreen forests of north-east India (0.624) coincided with relatively high annual total precipitation (2,238.9 mm). The time lags had a strong influence in the correlation coefficients between annual total rainfall and NDVI. The correlation coefficients were found to be comparatively high (R²=0.635) for dry deciduous forests than for evergreen forests and mixed deciduous forests, when the precipitation data with a lag of 30 days was correlated against NDVI. Using multiple regression approach models were developed for individual forest types using 16 different climatic indices. A high proportion of the temporal variance (>90%) has been accounted for by three of the precipitation parameters (maximum precipitation, precipitation of the wettest quarter and driest quarter) and two of the temperature parameters (annual mean temperature and temperature of the coldest quarter) for mixed deciduous forests. Similarly, in the case of deciduous forests, four precipitation parameters and three temperature parameters explained nearly 83.6% of the variance. These results suggest differences in the relationship between NDVI and climatic variables based upon the time of growing season, time interval and climatic indices over which they were summed. These results have implications for forest cover mapping and monitoring in tropical regions of India.     

A report on the conservation status of south Indian plants

Data on the conservation status of 167 species of flowering plants presented here are from two representative sectors in south India: (a) the lowlands, characterized by dry deciduous vegetation representative of the Deccan plateau, as also a cluster of eight low altitude hill ranges [to 1200 (1500) m] of the southernmost reaches of the Eastern Ghats of India, and coastlands including mangroves; and (b) the hills, the high altitude hill ranges (to 2475 m) of the Palni hills, an eastward spur of the Western Ghats of India, characterized by evergreen vegetation but largely decimated around the hill station of Kodaikanal with the invasion of alien species since the l840s with the arrival of overseas colonial personnel and the raising of commercial monoculture plantations. Data for the 167 species are entered in 7 + 1 columns for the two regions separately. However, the IUCN red list categories are not used in their entirety for two reasons: (i) the areas are relatively small; (ii) the stress comes more from extrinsic causes like habitat destruction and over-exploitation rather than intrinsic (genetic) causes. However, it is stressed that what is happening over restricted areas over short time intervals is a miniature of what is happening over larger areas over longer periods, and hence these data are vitally important in planning for conservation and eco-restoration. The quality and quantity of new information are decisive; the findings are discussed under nine heads, as the first synthesis of results obtained from over two decades of field work.     

The shrubs of the Western Ghats (South India): floristics and status

Abstract. This study examines the understorey shrub community in a wet evergreen forest of South India. The shrub community in the evergreen forests of the Western Ghats, Eastern Ghats, Sri Lanka, South-east Asia, Neotropics, Africa and Madagascar are compared. The shrub community is richer in the Old World Tropics as compared to the Neotropics. The common families in the Old World Tropics are: Rubiaceae, Acanthaceae, Euphorbiaceae, Myrsinaceae and Annonaceae. The number of families constituting the understorey shrub community falls from forty-six (Western Ghats) to thirteen (Central Amazon). The study concludes that the evergreen forests in the Western Ghats probably have the richest understorey shrub community.
Résumé. L'étude concerne la communauté d'arbustes de sous-bois d'une forêt sempervirente du Sud de l'lnde. Les communautés d'arbustes dans les forèts sempervirentes des Ghâts Occidentaux, des Ghâts Orientaux, du Sri Lanka, du Sud-Est Asie, des Néotropiques, de l'Afrique et de Madagascar sont comparées. La communauté d'arbustes est plus riche dans le Vieux Monde Tropical que dans les Néotropiques. Les families communes dans le Vieux Monde Tropical sont; Rubiaceae, Acanthaceae, Euphorbiaceae, Myrsinaceae et Annonaceae. Le nombre de families constituant la communauté d'arbustes de sous-bois varie de 46 (Ghâts Occidentaux) à 13 (Amazonie Centrale). L'étude conclue que les forêts sempervierentes des Ghàts Occidentaux ont probablement la plus riche communauté d'arbustes de sois-bois.
Mots clés. Communauté d'arbustes de sous-bois, forêts sempervirentes, Ghâts Occidentaux, endémisme, Inde.     

The diversity of life-form type, habitat preference and phenology of the endemics in the Goa region of the Western Ghats, India

Aim To carry out (1) a floristic survey of endemic flowering plants of the Western Ghats occurring in Goa, (2) identify their habitat preference and diversity of life‐form type, (3) observe flowering phenology of the endemics and (4) to correlate factors that affect their phenological pattern. Location Goa state is located between 15°48′ N and 14°53′54′′ N and 74°20′13′′ E and 73°40′33′′ E, in the northern part of the Western Ghats, India. Method A list of endemic plants from the study area was prepared using available floristic works and checklists of endemic plants of India. Based on preliminary field observations carried out in the study area, major habitats such as plateaus, moist deciduous forests, semi‐evergreen forests, evergreen forests and mangroves were identified for the subsequent intensive survey of endemics. Voucher specimens for all the endemic species were collected, processed using conventional herbarium techniques and deposited in the Herbarium, Department of Botany, Goa University. Species were identified using local and regional floras and their identity was confirmed at various herbaria along the Western Ghats. Data on their life‐form types, habitat and phenology was recorded in the field. Phenological observations were made every fortnight. A computerized data base was generated incorporating details on their life‐form type, phenology and habitat. Results A floristic survey of endemic plants of the Western Ghats in Goa resulted in the collection of 113 endemic species. Life‐form analysis reveals that herbaceous endemics are the most dominant followed by trees, shrubs and climbers. Plateaus in the study area harbour the largest number of endemic species, especially herbs. Endemic trees are distributed in the semi‐evergreen and evergreen forests. Endemic species in the study area show different peak and lean seasons of flowering depending on their life‐form type, habitat and ecological factors like temperature and rainfall/moisture content in the soil. Main conclusions The plateaus in the northern part of the Western Ghats are unique, being species rich with herbaceous endemics. These ephemerals are closely associated with the rainfall patterns thus; any change of moisture regime over the long‐term will have an impact on the distribution of these endemics.     

Socio-cultural protection of endemic trees in humanised landscape

Culturally protected forest patches or sacred groves have been the integral part of many traditional societies. This age old tradition is a classic instance of community driven nature conservation sheltering native biodiversity and supporting various ecosystem functions particularly hydrology. The current work in Central Western Ghats of Karnataka, India, highlights that even small sacred groves amidst humanised landscapes serve as tiny islands of biodiversity, especially of rare and endemic species. Temporal analysis of landuse dynamics reveals the changing pattern of the studied landscape. There is fast reduction of forest cover (15.14–11.02 %) in last 20 years to meet up the demand of agricultural land and plantation programs. A thorough survey and assessment of woody endemic species distribution in the 25 km² study area documented presence of 19 endemic species. The distribution of these species is highly skewed towards the culturally protected patches in comparison to other land use elements. It is found that, among the 19 woody endemic species, those with greater ecological amplitude are widely distributed in the studied landscape in groves as well as other land use forms whereas, natural population of the sensitive endemics are very much restricted in the sacred grove fragments. The recent degradation in the sacred grove system is perhaps, due to weakening of traditional belief systems and associated laxity in grove protection leading to biotic disturbances. Revitalisation of traditional practices related to conservation of sacred groves can go a long way in strengthening natural ecological systems of fragile humid tropical landscape.     

Healing Animals,Feeding Souls: Ethnobotanical Values at Sacred Sites in Central Italy

Healing Animals, Feeding Souls: Ethnobotanical Values at Sacred Sites in Central Italy. Ethnobotanical knowledge is a fundamental repository of the values and applications of different plants. This knowledge is often related to spiritual beliefs and religious sites, where plants have been nurtured and conserved for their use in rituals and traditional practices. While this link is well known for different areas of the global south, it has hardly been investigated in relatively more secular and modernized Western contexts. Here, we use first–hand vegetation surveys and published records to examine the occurrence of ethnobotanical values at 30 Catholic shrines in Central Italy, and compare them with an equal number of non–sacred control sites. We ask this: to what extent is there an association of useful plants with sacred places in Italy, as found in other cultural contexts? We show that a greater number of useful plants are found at sacred sites. While this is mainly a consequence of the higher species richness of sacred sites, an association with plants used in animal husbandry is particularly evident, and likely related to the deep historical connection between sacred places and pastoralist traditions in Central Italy. Also, we show that there are significant variations in the distribution of old trees; the largest specimens are found at the center of sacred sites, while tree size visibly decreases away from the shrines. This indicates also that individual trees have been actively managed and conserved at sacred sites, probably driven by the symbolic values that old trees frequently embody.     

Distribution, endemism and threat status of freshwater fishes in the Western Ghats of India

Aim To study (1) the large-scale distribution patterns of freshwater fishes in the Western Ghats of India; (2) the endemism and uniqueness of the fishes in various zones; and (3) the threat status of fishes by categorizing them under low risk (LR), vulnerable (VU), endangered (EN) and critically endangered (CR). Location The Western Ghats of India. Methods The scientific literature describing the freshwater fishes of the Western Ghats was reviewed. Data describing the lists of the species were extracted and complied. The species accumulation curve was plotted using Michaelis–Menten-like equation. The Western Ghats was divided into six zones and similarity of the species was calculated using Jacquard's index. Results Literature to date records 288 species belonging to 12 orders, 41 families and 109 genera, of which 118 species are endemic and 51 are unique. However, the species accumulation curve shows that there might be 345 species in this region, indicating that 16% species have not been recorded to date. An analysis of the distribution pattern of fishes in the Western Ghats suggests that the southern region is more diverse than the northern and central regions. The southern region shows high endemism and high uniqueness while the northern region shows high endemism but less uniqueness. The similarity index between the zones indicates that as the distance between the zones increases similarity decreases. The status of 105 of 288 species was not known due to data deficiency but among the remaining 183 species, 58 species were categorized as LR, 41 as VU, 54 as EN, 24 as CR while the remaining six species were introduced. Conclusions The distribution patterns of fishes in the Western Ghats are discussed in accordance with the geography of Western Ghats, its climatic conditions and ‘Satpura Hypothesis’. The threat status of fishes found in Western Ghats suggests that at least 41% of fish fauna is threatened by either being VU, EN or CR. Implication of potent conservation measures is necessary to conserve the fish fauna of Western Ghats.     

Endemism and sexual systems in the evergreen tree flora of the Western Ghats, India

This study reports the endemism and sexual system in the wet evergreen tree flora of the Western Ghats. A total of 656 species from 66 families and 231 genera were listed. This included a gymnosperm family (Podocarpaceae) and a monocot family (Arecaceae). No family was endemic to the Western Ghats, but 352 species (53%) from 43 families and five genera were endemic. Nearly 35% of the families had no endemics. The largest families with endemics were Dipterocarpaceae (92%), Anacardiaceae (84%), Lauraceae (72%), Fabaceae, Rubiaceae and Myrtaceae (68%). The top five contributing families in the tree flora of the Western Ghats were Euphorbiaceae, Lauraceae, Rubiaceae, Myrtaceae and Annonaceae. The 656 species were largely hermaphrodites (57%) followed by dioecious (20%), polygamous (16%) and monoecious species (5%). The rate of dioecy reported in this study (20%) is higher than reports for Puerto Rico (18%) but lower than the Malaysian rainforest (26%). Structurally, like the Neotropical forests, most evergreen forest types of the Western Ghats could be classified into four ensembles. Yet, the Western Ghats had fewer species than other tropical and Neotropical forests. The proportion of endemics in the ensembles of the Western Ghats ranged from 34% (ensemble IV) to 14% (ensemble I).     

云南楚雄彝族的“神树林”与生物多样性保护

所述的“神树林”是广泛意义上的概念,包括多种因文化信仰而保护的各种森林块.这些森林块不仅有多种的生态功能,也是村社水平生物多样性相对集中的地方.通过对云南楚雄彝族的“神树林”进行了广泛调查,取样比较了自然保护区、村社集体林和“神树林”3种不同管理模式下的森林群落的植物物种多样性,结果表明“神树林”群落的物种总数(67)、样方特有种(17)、Shannon-Wiener物种多样性指数(2.96)都显著高于自然保护区群落(分别为44、8、2.17)和集体林群落(分别为34、4、2.39),表明云南楚雄彝族的“神树林”在当地生物多样性保护和管理中有着不可低估的作用.     

云南楚雄彝族的"神树林"与生物多样性保护

所述的“神树林”是广泛意义上的的概念,包括多种因文化信仰面保护的各种森林块,这些森林块不仅有多种的生态功能,也是村社水平生物多样性相对集中的地方,通过对云南楚雄彝族的“神树林”进行了广泛调查,取样比较了自然保护区,村社集体林和“神树林”３种不同管理模式下的森林群落的植物物种多样性,结果表明“神树林”群落的物种总数的植物物种多样性,结果表明“神树林”群落的物种总数（６７）、样方特有种（１７）、Ｓｈａ     

Catholicism and Conservation: The Potential of Sacred Natural Sites for Biodiversity Management in Central Italy

The connection between religion, nature and conservation has become a prominent topic among scholars and conservation practitioners. Numerous studies have shown that spiritual beliefs have contributed to preserving important biodiversity in sacred areas around the world. In Western contexts, however, that link has been underexplored, perhaps due to a common view of Christianity as anti-naturalistic. Here, I rely on a literature review and first-hand observations to identify patterns and trends characterizing Catholic sacred sites in Central Italy. I show that a high proportion of the sites are located in natural areas, and that some types of sites and strands of Catholicism are associated with natural settings more frequently than others. Further, these natural sacred sites often display ecological features that highlight their important conservation role. Greater awareness and consideration of local spiritual heritages are recommended to guarantee more effective and integrated management of the sites.     

A method for assessing evergreen habitats using phytodiversity and geospatial techniques in tropical rain forests of Southern Western Ghats (India)

We have used data generated using remote sensing and geographical information systems to categorize habitats, and then determined the relationship between the habitat categorizations and species-distribution patterns. A biologically rich hotspot—Kalakad-Mundanthurai Tiger Reserve, located at Southern Western Ghats, India, was chosen for this study. In order to spatially delineate areas of high species richness/diversity and endemic habitat zones, we have identified evergreen habitats in conjunction with landscape metrics, species assemblage, micro-habitats like slope, topography, species endemism, and proportion of core and edge species. A total of 236 species and 2,920 individuals were recorded using systematic stratified plots of 0.1 ha covering 47 plots. Hierarchical cluster analysis was done using Ward’s method. Plot information was used to identify clusters based on species density. The analysis showed five species assemblages that are quite distinct from each other in terms of dominant species. The distribution of endemic and edge species, land cover heterogeneity, and continuity of patches in these clusters were evaluated to understand the degree of disturbance and intactness at landscape scale. Integration of species assemblages and topography brought out four major elevation-slope complexes. Information on species composition (robust field survey) with spectral (hybrid classification) properties has shown 72% overall accuracy and distinguished four evergreen sub-groups and other land cover classes. The developed approach assumes great importance in the assessment of biodiversity and prioritizing the areas of conservation.     

Spatial analysis of endemism to redefine conservation areas in Western Ghats (India)

A meaningful effort for the preservation of endemism would require a deep understanding of its related mechanisms and an accurate estimation of its spatial distribution. Here, we applied methods dedicated to species distribution modelling (SDM) to map an integrated index in India's Western Ghats biodiversity hotspot, the endemic tree richness, and to use it for recommendations of protected areas. We then rigorously compared SDM results with spatially explicit and multiscale comparison tools, among them the cutting-edge correlation map and profile (CMP) technique, to finally draw up an endemic richness map with improved accuracy.The endemic richness showed a sharply increasing southward gradient in the Western Ghats, mainly driven by the seasonality of the temperature and the precipitation's stability. This precise quantification of the tree endemism pattern in peninsular India helped in identifying vulnerable areas in terms of conservation of biodiversity as a whole. The Indian authorities recently used our recommendations to extend protected areas in the southern tip of the Indian peninsula to conserve this endemic richness. We believe that spatial analyses and multiscale comparison tools such as those presented here can help conservationists everywhere to better cope with the difficulties met in identifying zones for protected status.     

Sacred groves of Manipur,northeast India:biodiversity value,status and strategies for their conservation

The people of Manipur, a state in northeast India, follow ancestral worship and animism in the form of deity worship, with the central focus on worship in forest patches. The beliefs and taboos associated with the Sylvan deities (Umanglais) in the forest patches are restricted to any sort of disturbance of flora and fauna. These social boundaries help to conserve the entire organism as a whole, which stand the concept of sacred groves. The pleasing of deities is performed every year by the Meiteis, a dominant community of Manipur, in honour of the deities and to gain their favour. Indigenous cultural and rituals practices of the local people in sacred groves serve as a tool for conserving biodiversity. Sacred groves are distributed over a wide ecosystem and help in conservation of rare and endemic species. Well-preserved sacred groves are store houses of valuable medicinal and other plants having high economic value, and serve as a refuge to threatened species. One hundred and sixty-six sacred groves were inventoried in Manipur valley that comprises Imphal east, Imphal west, Thoubal and Bishnupur districts of the state. Detailed studies were carried out in four selected sacred groves, to know the importance of biodiversity status and vegetation characteristics. A total of 173 plant species representing 145 genera under 70 families were recorded through baseline floristic survey. The species diversity indices were compared among the four studied groves. The vegetation composition and community characteristics were recorded. Ethnobotanical uses of species were examined, which reveal that 96% of the species were used as medicine for the treatment of various ailments. Utilization of herbal medicine by the Meiteis is closely related to the cultural and ritual practices. A few of the medicinal plants which have disappeared from the locality are now confined only to the groves. Socio-cultural aspects were investigated taking into account the attitudes of local people, which indicate social beliefs and taboo are eroding, simultaneously degrading the degree of protection of sacred groves. Therefore, conservation measures of sacred groves need to be formulated considering the factor of degradation and the basic necessities of the local people. Until and unless a viable option is provided to the local people (especially those who habitat nearby the adjoining areas) for sustaining their economic condition, no step for conservation of biodiversity will be successful.     

Distribution,Demography, and Conservation of Lion-tailed Macaques (Macaca silenus) in the Anamalai Hills Landscape,Western Ghats,India

The status of the endemic and endangered lion-tailed macaque (Macaca silenus) has not been properly assessed in several regions of the Western Ghats of southern India. We conducted a study in Parambikulam Forest Reserve in the state of Kerala to determine the distribution, demography, and status of lion-tailed macaques. We laid 5km² grid cells on the map of the study area (644km²) and made four replicated walks in each grid cell using GPS. We gathered data on lion-tailed macaque group locations, demography, and site covariates including trail length, duration of walk, proportion of evergreen forest, height of tallest trees, and human disturbance index. We also performed occupancy modeling using PRESENCE ver. 3.0. We estimated a minimum of 17 groups of macaques in these hills. Low detection and occupancy probabilities indicated a low density of lion-tailed macaques in the study area. Height of the tallest trees correlated positively whereas human disturbance and proportion of evergreen forest correlated negatively with occupancy in grid cells. We also used data from earlier studies carried out in the surrounding Anamalai Tiger Reserve and Nelliyampathy Hills to discuss the conservation status in the large Anamalai Hills Landscape. This landscape harbors an estimated population of 1108 individuals of lion-tailed macaques, which is about one third of the entire estimated wild population of this species. A conservation plan for this landscape could be used as a model for conservation in other regions of the Western Ghats.     

Endemic Asian Chytrid Strain Infection in Threatened and Endemic Anurans of the Northern Western Ghats,India

The Western Ghats of India harbors a rich diversity of amphibians with more than 77% species endemic to this region. At least 42% of the endemic species are threatened due to several anthropogenic stressors. However, information on amphibian diseases and their impacts on amphibian populations in this region are scarce. We report the occurrence of Batrachochytridium dendrobatidis (Bd), an epidermal aquatic fungal pathogen that causes chytridiomycosis in amphibians, from the Western Ghats. In the current study we detected the occurrence of a native Asian Bd strain from three endemic and threatened species of anurans, Bombay Night Frog Nyctibatrachus humayuni, Leith''s Leaping Frog Indirana leithii and Bombay Bubble Nest Frog Raorchestes bombayensis, for the first time from the northern Western Ghats of India based on diagnostic nested PCR, quantitative PCR, DNA sequencing and histopathology. While, the Bd infected I. leithii and R. bombayensis did not show any external symptoms, N. humayuni showed lesions on the skin, browning of skin and sloughing. Sequencing of Bd 5.8S ribosomal RNA gene, and the ITS1 and ITS2 regions, revealed that the current Bd strain is related to a haplotype endemic to Asia. Our findings confirm the presence of Bd in northern Western Ghats and the affected amphibians may or may not show detectable clinical symptoms. We suggest that the significance of diseases as potential threat to amphibian populations of the Western Ghats needs to be highlighted from the conservation point of view.