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1.
1. The responses of rat chorda tympani nerve to various salts carrying cations of large molecular size which have small permeability were measured. 2. Salts carrying polyvalent cations such as Fe3+ or La3+ elicited much larger responses than NaCl or KCl. 3. Ammonium chloride derivatives having methyl or ethyl groups and salts carrying other organic cations of large molecular size elicited the responses comparable to that induced by NH4Cl or NaCl. 4. It was suggested that the taste responses to the salts carrying the cations of large molecular size are induced not by the cation transport but by adsorption of the cations on the membranes.  相似文献   

2.
Cross-adaptation has occurred when exposure to an adapting chemicalstimulus (A) reduces the response to a subsequent test stimulus(B). The degree of cross-adaptation between two stimuli is thoughtto reflect the overlap of their ‘neural activation processes’.We measured self- (A—A) and reciprocal crossadaptation(A—B, B—A) of the response of the hamster chordatympani nerve with lingual presentations of stimuli elicitingequal unadapted transient responses. Adapting and test stimuliwere 0.1 M NaCl, 0.1 M NaNO3, 0.1 M NaBr, 0.4 M Na acetate (NaAc),0.09 M LiCl and 0.4 M NH4Cl. Nearly complete and symmetricalcross-adaptation was seen for NaCl, NaNO3 and NaBr. Those Nasalts paired with LiCl showed strong but asymmetrical cross-adaptation.Exposure to sodium completely eliminated the response to LiClbut not vice versa, suggesting that lithium and sodium are notcompletely interchangeable taste stimuli for the hamster chordatympani. Relatively little cross-adaptation between NH4Cl andother salts suggested relatively separate neural activationprocesses. Strongly asymmetrical cross-adaptation was foundbetween NaAc and the other sodium salts. Responses to NaCl,NaNO3 or NaBr were eliminated after adaptation to NaAc whereasthe response to NaAc during the reciprocal cross was strong.Asymmetries are discussed in reference to sensitivities of singlenerve fibers for the chorda tympani, effects of adaptation andthe concept of anion inhibition.  相似文献   

3.
Chemical defenses are used by many organisms to avoid predation, and these defenses may function by stimulating predators’ chemosensory systems. Our study examined detection mechanisms for components of defensive ink of sea hares, Aplysia californica, by predatory sea catfish, Ariopsis felis. Behavioral analyses show aplysioviolin and phycoerythrobilin are detected intra-orally and by barbels and are deterrent at concentrations as low as 0.1% full strength. We performed electrophysiological recordings from the facial–trigeminal nerve complex innervating the maxillary barbel and tested aplysioviolin, phycoerythrobilin, amino acids, and bile salts in cross-adaptation experiments. Amino acids and bile salts are known stimulatory compounds for teleost taste systems. Our results show aplysioviolin and phycoerythrobilin are equally stimulatory and completely cross-adapt to each other’s responses. Adaptation to aplysioviolin or phycoerythrobilin reduced but did not eliminate responses to amino acids or bile salts. Adaptation to amino acids or bile salts incompletely reduced responses to aplysioviolin or phycoerythrobilin. The fact that cross-adaptations with aplysioviolin and phycoerythrobilin were not completely reciprocal indicates there are amino acid and bile salt sensitive fibers insensitive to aplysioviolin and phycoerythrobilin. These results indicate two gustatory pathways for aplysioviolin and phycoerythrobilin: one independent of amino acids and bile salts and another shared with some amino acids.  相似文献   

4.
Large enhancement of canine taste responses to sugars by salts   总被引:1,自引:0,他引:1       下载免费PDF全文
The effects of changed ionic environments on the canine taste responses to sugars were examined by recording the activity of the chorda tympani nerve. a) The responses to various sugars were greatly enhanced by the presence of salts having monovalent cations such as Na+, K+, choline+, or Tris+. The responses to sugars were suppressed by high concentrations of salts. (b) The presence of 100 mM NaCl in fructose solution did not affect the maximal response and changed the Hill constant for the concentration-response relationship from 1.3 to 2.4. (c) CaCl2 greatly enhanced the response to fructose, while MgCl2 exhibited practically no effect. The presence of 20 mM CaCl2 in fructose solution changed the Hill constant from 1.2 to 2.4. (d) CaCl2 suppressed the responses to 0.5 M sugars except for fructose and sucrose and enhanced the responses to all sugars examined at 1 M. In the glucose response, the slope of the concentration-response curve was increased by the presence of CaCl2. Here the curve in the absence of CaCl2 intersected with that in the presence of CaCl2, indicating that CaCl2 suppressed the response to glucose of low concentrations and enhanced that of high concentrations. (e) The enhancement of the sugar responses by salts was not simply explained in terms of ionic permeability at the apical membranes of taste cells. The enhanced and suppressed effects of salts on the sugar responses were interpreted in terms of the cooperativity between receptor molecules for sugars.  相似文献   

5.
Arthur Wallace 《Plant and Soil》1970,32(1-3):526-530
Summary Bush bean plants were exposed to either Rb86 or Cs137 for 24 hours with different monovalent cations as carriers in single-salt solutions except for the presence of 10−4 M CaCl2. Ratio of uptake of the radionuclides at 10−3 to 10−2 M was used as an index of the carrier ability of various cations. Different monovalent cations decreased uptake of Cs137 and its transport to shoots unequally when 10−2 M salts were compared with 10−3 M salts. Rubidium and cesium salts decreased Cs137 uptake equally but potassium salts were less effective in decreasing uptake when the ratios of the two concentrations were considered. All monovalent cations decreased uptake of Cs137 at the 10−2 M carrier concentration but some did not at 10−3 M. Nitrate nitrogen was a big factor in these results. Cesium and rubidium salts were most effective. Potassium appeared to increase Cs137 transport to shoots particularly at 10−3 M KNO3. Only cesium, rubidium, and potassium salts decreased uptake of Rb86 when 10−2 M salts were compared with 10−3 M. Rubidium and cesium salts decreased uptake essentially equally and potassium salts again were less effective. All nitrate salts tended to increase Rb86 transport to shoots more consistently than with Cs137. It is concluded that absorption and transport to shoots were not equivalent for potassium, rubidium, and cesium.  相似文献   

6.
The effect of calcium-sensing receptor (CaR) agonists on frog gustatory responses was studied using glossopharyngeal nerve recording and whole-cell patch-clamp recording of isolated taste disc cells. Calcimimetic NPS R-467 dissolved in normal saline solution elicited a large transient response in the nerve. The less active enantiomer of the compound, NPS S-467 induced only a small neural response. The EC50 for NPS R-467 was about 20 μM. Cross-adaptation experiments were performed to examine the effect of 30 μM NPS R-467 and 100 μM quinine on the gustatory neural response. The magnitude of the R-467-induced response after adaptation to quinine was approximately equal to that after adaptation to normal saline solution, indicating that the receptor site for NPS R-467 is different from the site for quinine. NPS R-467 (100 μM) also induced an inward current accompanied with conductance increase and large depolarization in two (13%) of 15 rod cells, and a sustained decrease in outward current and small depolarization in six (40%) other rod cells. NPS S-467 (100 μM) induced a sustained decrease in outward current and depolarization in five (50%) of 10 rod cells. Another calcimimetic cinacalcet (100 μM) induced an inward current accompanied with conductance increase in three (27%) of 11 rod cells. The results suggest that NPS R-467 induces neural responses through cell responses unrelated to a resting K+ conductance decrease.  相似文献   

7.
The role of tight junctions in modulating responses from chorda tympani (taste) and lingual (general sensory) nerves are clarified in regard to their responses to salts. Chorda tympani (CT) responses elicited by organic sodium salts require greater Na+ concentrations to elicit the same magnitude of response as NaCl. These data can be understood in terms of the organic anions (compared with Cl) producing larger liquid-junction potentials across tight junctions between taste cells which, in turn, reduces Na+ influx into taste cells via amiloride-inhibitable channels. The anion contribution to the CT response to different Na+ salts can be eliminated (or enhanced) by voltage clamping the tongue with negative (with respect to the serosal solution) potentials.Whole nerve recordings from the lingual branch of the trigeminal nerve elicited by NaCl (and other salts) were reversibly inhibited by the tight junction blocker, LaCl3 These data suggest that small hydrophilic molecules elicit responses from trigeminal fibers by diffusing across tight junctions between epithelial cells and altering the composition of the extracellular space.  相似文献   

8.
In a previous paper (Nomura and Kurihara (1987) Biochemistry 26, 6135-6140), we demonstrated that the lipid bilayer membranes exhibit membrane potential changes in response to various odorants similarly to olfactory cells. The present study demonstrated that ion dependence of the responses of the lipid membranes to odorants is related to that in the carp olfactory system in the following point. (1) The responses to odorants diminished at low concentrations of salts and recovered upon addition of salts to stimulating solutions. (2) Divalent cations were effective in supporting the responses to odorants at much lower concentrations that monovalent cations. (3) Impermeable organic cations were effective in supporting the responses. The present results suggest that the initial process of generation of the receptor potential in olfactory cells resembles that in the lipid membranes.  相似文献   

9.
The effect of ethanol on the amiloride- and benzamil (Bz)-insensitive salt taste receptor was investigated by the measurement of intracellular Na(+) activity ([Na(+)](i)) in polarized rat fungiform taste receptor cells (TRCs) using fluorescence imaging and by chorda tympani (CT) taste nerve recordings. CT responses were monitored during lingual stimulation with ethanol solutions containing NaCl or KCl. CT responses were recorded in the presence of Bz (a specific blocker of the epithelial Na(+) channel [ENaC]) or the vanilloid receptor-1 (VR-1) antagonists capsazepine or SB-366791, which also block the Bz-insensitive salt taste receptor, a VR-1 variant. CT responses were recorded at 23 degrees C or 42 degrees C (a temperature at which the VR-1 variant salt taste receptor activity is maximally enhanced). In the absence of permeable cations, ethanol induced a transient decrease in TRC volume, and stimulating the tongue with ethanol solutions without added salt elicited only transient phasic CT responses that were insensitive to elevated temperature or SB-366791. Preshrinking TRCs in vivo with hypertonic mannitol (0.5 M) attenuated the magnitude of the phasic CT response, indicating that in the absence of mineral salts, transient phasic CT responses are related to the ethanol-induced osmotic shrinkage of TRCs. In the presence of mineral salts, ethanol increased the Bz-insensitive apical cation flux in TRCs without a change in cell volume, increased transepithelial electrical resistance across the tongue, and elicited CT responses that were similar to salt responses, consisting of both a transient phasic component and a sustained tonic component. Ethanol increased the Bz-insensitive NaCl CT response. This effect was further enhanced by elevating the temperature from 23 degrees C to 42 degrees C, and was blocked by SB-366791. We conclude that in the presence of mineral salts, ethanol modulates the Bz-insensitive VR-1 variant salt taste receptor.  相似文献   

10.
Recent investigations of amino acid taste responses in fishare reviewed, with emphasis on structure–activity relationships,receptor types and response enhancements. High stereospecificityof taste receptors in fishes is evidenced by response to L-aminoacids of low mol. wt containing three to four carbon atoms,along with L-arginine, a six-carbon positively charged aminoacid. Enantiomers are generally nonstimulatory. Attention isalso informatively given to cross adaptation, synergistic andmixture experiments.  相似文献   

11.
The resistance to detergents and detergent-induced tolerance of a gastrointestinal organism, Enterococcus faecalis ATCC 19433, were examined. The most remarkable observation was the rapid response of cells in contact with bile salts and sodium dodecyl sulfate (SDS). The killing by high concentrations of detergents was nearly instantaneous. A 5-s adaptation with moderate sublethal concentrations of bile salts or SDS (0.08 or 0.01%, respectively) was sufficient to induce significant adaptation against homologous lethal conditions (0.3% bile salts or 0.017% SDS). However, resistance to a subsequent lethal challenge progressively increased further to a maximum reached after 30 min of adaptation. Furthermore, extremely strong cross-resistances were observed with bile salts- and SDS-adapted cells. However, no relationship seems to exist between levels of tolerance and de novo-synthesized proteins, since blockage of protein synthesis during adaptation had no effect on induction of resistance to bile salts and SDS. We conclude that this induced tolerance to detergent stress is independent of protein synthesis. Nevertheless, the stress-induced protein patterns of E. faecalis ATCC 19433 showed significant modifications. The rates of synthesis of 45 and 34 proteins were enhanced after treatments with bile salts and SDS, respectively. In spite of the overlap of 12 polypeptides, the protein profiles induced by the two detergents were different, suggesting that these detergents trigger different responses in E. faecalis. Therefore, bile salts cannot be substituted for SDS in biochemical detergent shock experiments with bacteria.  相似文献   

12.
Summary The sum of Na, K, Ca, Mg in the exudate of tobacco generally exceeded the sum of mineral anions. Insufficient organic acids were present to account for the differences and bicarbonate appeared to be the other anion involved. Amino acids were present in very low concentrations relative to mineral cations. When nitrate salts only were in the external solutions, the anions were mostly, but not entirely, nitrate. When chloride salts only were in the external solutions, the cations far exceeded the level of mineral anions in the exudate. It is postulated that nitrate is actively transported when nitrate salts are in the external solution regardless of the cation, but when anions other than nitrate are in the external solution, the cations are actively transported with the anions passively following. Nitrate transport was via a symplasm, but that of the other anions seemed to be different. When bicarbonate is the only anion in the external solution and when present at relatively high concentrations (5 × 10−3 M or higher), the volume of exudate is decreased. It appears that the organic acids which were synthesized as a result of the bicarbonate absorption were not transferred to the xylem vessels.  相似文献   

13.
Summary Using rapid ionic fixation with molybdic or tungstic heteropolyanions (strong precipitating agents of quaternary ammonium cations such as choline and acetylcholine), acetylcholine-like cations were localized aspoint-like precipitates in the synaptic vesicles of resting (electrically nonstimulated) motor nerve terminals. When performed at low temperature, the same procedure revealedspot-like precipitates (presumed to be exocytotically released acetylcholine-like cations) in the synaptic cleft in the vicinity of the active zone. These precipitates were often seen in paired forms. Unlike resting motor-nerve terminals, excited terminals (electrical stimulation with occasional 4-aminopyridine pretreatment) after ionic fixation exhibited, at first,laminar precipitates both in the vicinity of the active zone inside the nerve terminals and in the synaptic space. In the vicinity of the active zone, the laminar precipitates were directed towards the synaptic membrane, while those in the synaptic space showed no orientation. Ionic fixation also revealeddiffused precipitates both around the synaptic vesicles and on the axoplasmic side of the presynaptic membrane. Finally, the same fixation procedure demonstrated the presence of empty synaptic vesicles (without point-like precipitates) in close contact with the presynaptic membrane. The laminar and diffused precipitates are presumed to be two different forms of the same salts of acetylcholine-like cations that are insolubilized by ionic fixation in both the nerve terminals and the synaptic space of excited motor end-plates.  相似文献   

14.
A chemotactic behaviour in Drosophila larvae to monovalent salts was investigated. Larvae showed clear-cut bimodal responses; they preferred low concentration of salts and rejected high. For example, they were attracted to solutions over the range of concentrations of NaCl from 10?4 M to 10?1 M but avoided concentrations above 2 × 10?1 M.The responses to KCl, KBr and Kl showed differences in attraction whereas avoidance thresholds were similar. On the other hand, when the responses to ChCl and ChBr were compared with those to KCl and KBr respectively, only the avoidance responses were depressed. Furthermore, from choice experiments between KCl, NaCl and ChCl, it was shown that cations did not affect the attraction responses.The results observed strongly suggest the existence of two salt receptors (or receptor sites) which act antagonistically on the chemotaxis.  相似文献   

15.
Electroretinograms were recorded from the horseshoe crab compound eye using a high-intensity light-emitting diode and a whole-eye seawater electrode. Recordings were made from both lateral eyes in natural daylight or in continuous darkness with the optic nerve intact or cut. Recordings from two eyes of the same animal in different conditions facilitated direct comparisons of the effects of diurnal lighting and circadian efferent activity on the daily patterns of sensitivity of the eye. Structural changes appear to account for about half of the total electroretinogram excursion. Circadian input begins about 45 min in advance of sunset and the nighttime sensitivity returns to the daytime values 20 min after sunrise. When the optic nerve is cut, the nighttime sensitivity shows exponential decay over the next 5 or 6 days, consistent with a light-triggered structural light adaptation process unopposed by efferent input. Our results suggest that two mechanisms mediate the increase in lateral eye sensitivity at night—physiological dark adaptation and circadian efferent input. Three mechanisms appear to be involved in mediating the decrease in lateral eye sensitivity during daylight—physiological light adaptation, a continuous structural light adaptation process, and a separate light-triggered, efferent-primed structural light adaptation process.  相似文献   

16.
The effect of various substances on living cells may be advantageously studied by exposing them to such substances and observing their subsequent behavior in solutions of a basic dye, brilliant cresyl blue. The rate of penetration of the basic dye, brilliant cresyl blue, is decreased when cells are exposed to salts with monovalent cations before they are placed in the dye solution (made up with borate buffer mixture). This inhibiting effect is assumed to be due to the effect of the salts on the protoplasm. This effect is not readily reversible when cells are transferred to distilled water, but it is removed by salts with bivalent or trivalent cations. In some cases it disappears in dye made up with phosphate buffer mixture, or with borate buffer mixture at the pH value in which the borax predominates, and in the case of NaCl it disappears in dye containing NaCl. No inhibiting effect is seen when cells are exposed to NaCl solution containing MgCl2 before they are placed in the dye solution. The rate of penetration of dye is not decreased when cells are previously exposed to salts with bivalent and trivalent cations. The rate is slightly increased when cells are placed in the dye solution containing a salt with monovalent cation and probably with bivalent or trivalent cations. In the case of the bivalent and trivalent salts the increase is so slight that it may be negligible.  相似文献   

17.
S Tsuji 《Histochemistry》1985,83(3):213-219
Using rapid ionic fixation with molybdic or tungstic heteropolyanions (strong precipitating agents of quaternary ammonium cations such as choline and acetylcholine), acetylcholine-like cations were localized as point-like precipitates in the synaptic vesicles of resting (electrically nonstimulated) motor nerve terminals. When performed at low temperature, the same procedure revealed spot-like precipitates (presumed to be exocytotically released acetylcholine-like cations) in the synaptic cleft in the vicinity of the active zone. These precipitates were often seen in paired forms. Unlike resting motor-nerve terminals, excited terminals (electrical stimulation with occasional 4-aminopyridine pretreatment) after ionic fixation exhibited, at first, laminar precipitates both in the vicinity of the active zone inside the nerve terminals and in the synaptic space. In the vicinity of the active zone, the laminar precipitates were directed towards the synaptic membrane, while those in the synaptic space showed no orientation. Ionic fixation also revealed diffused precipitates both around the synaptic vesicles and on the axoplasmic side of the presynaptic membrane. Finally, the same fixation procedure demonstrated the presence of empty synaptic vesicles (without point-like precipitates) in close contact with the presynaptic membrane. The laminar and diffused precipitates are presumed to be two different forms of the same salts of acetylcholine-like cations that are insolubilized by ionic fixation in both the nerve terminals and the synaptic space of excited motor end-plates.  相似文献   

18.
Kitada  Yasuyuki 《Chemical senses》1989,14(4):487-502
In the frog glossopharyngeal nerve, single water fibers respondto low CaCl2 (1–2 mM) and relatively high MgCl2 (100 mM).In the present study, it was found that stimulation by a mixtureof low CaCl2 and relatively high MgCl2 led to a small response.This suggests that the Ca+ response is inhibited by the presenceof Mg2+ and the Mg2+ response is inhibited by the presence ofCa2+. Hence, it is suggested that there are different receptorsites for divalent cations in single water fibers of the frogglossopharyngeal nerve, a calcium receptor site (XCa) responsiblefor the Ca2+ response and a magnesium receptor site (XMg) responsiblefor the Mg2+ response. It has been reported that Mg2+ inhibitsthe Ca2+ response by competing with Ca2+ for XCa (Kitada andShimada, 1980). In the present study, the inhibition of theMg2+ response by Ca2+ was examined quantitatively under theassumption that the magnitude of the neural response is proportionalto the amount of MgXMg complex minus a constant (the thresholdconcentration of the MgXMg complex). The results obtained indicatethat Ca2+ competes with Mg2+ for XMg. The apparent dissociationconstants for MgXMg complex and CaXMg complex, which were obtainedfrom the present study, were 8.0 x 10–2 M and 7.2 x 10–4M, respectively. Thus, competition between Ca+ and Mg2+ forthe distinct receptor sites involved in taste reception wasdemonstrated by the results described in this paper. Since thedivalent cations do not always bring about activation of tastereceptors, the responses to salts in the frog glossopharyngealnerve cannot be explained in terms of changes in the surfacepotential outside the taste cells. The present results suggestthat there exist multiple specific receptor sites for cationsinvolved in salt taste responses, and only the binding of eachseparate cation to its appropriate receptor sites leads to activationof the receptor and the initiation of impulses in sensory nerveendings.  相似文献   

19.
The formation of complexes between mucopolysaccharides and tetrazolium salts has been studied both by turbidimetry and nephelometry. The technique of laser nephelometry allows the detection of colloidal aggregates in systems which may, by turbidimetric methods, be ambiguous. The results indicate that binding of tetrazolium salts to polyanions can result in soluble as well as insoluble complexes; the monotetrazolium salts form soluble complexes and the ditetrazolium compounds form insoluble complexes. The insoluble complexes are stable at relatively low pH, and are disrupted during reduction to the formazan. Complex formation is decreased at high ratios of heparin to tetrazolium, and divalent cations, even at high concentration, do not precipitate mucopolysaccharides. It is concluded that stable ionic interactions with spatial charge separation are responsible for the cross linking of the mucopolysaccharides and the formaiton of large insoluble aggregates.  相似文献   

20.
Inhibitions of 30?nM rabbit muscle 1-phosphofructokinase (PFK-1) by lithium, potassium, and sodium salts showed inhibition or not depending upon the anion present. Generally, potassium salts were more potent inhibitors than sodium salts; the extent of inhibition by lithium salts also varied with the anion. Li2CO3 was a relatively potent inhibitor of PFK-1 but LiCl and lithium acetate were not. Our results suggest that extents of inhibition by monovalent salts were due to both cations and anions, and the latter needs to be considered before inhibition can be credited to the cation. An explanation for monovalent salt inhibitions is proffered involving interactions of both cations and anions at negative and positive sites of PFK-1 that affect enzyme activity. Our studies suggest that lithium cations per se are not inhibitors: the inhibitors are the lithium salts, and we suggest that in vitro studies involving the effects of monovalent salts on enzymes should involve more than one anion.  相似文献   

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