Development of human health damage factors for PM<Subscript>2.5</Subscript> based on a global chemical transport model

Purpose

Health damage from ambient fine particulate matter (PM_2.5) shows large regional variations and can have an impact on a global scale due to its transboundary movement. However, existing damage factors (DFs) for human health in life cycle assessments (LCA) are calculated only for a few limited regions based on various regional chemical transport models (CTMs). The aim of this research is to estimate the human health DFs of PM_2.5 originating from ten different regions of the world by using one global CTM.

Methods

The DFs express changes in worldwide disability-adjusted life years (DALYs) due to unit emission of black carbon and organic carbon (BCOC), nitrogen oxides (NO _x ), and sulfur dioxide (SO₂). DFs for ten regions were calculated as follows. Firstly, we divided the whole world into ten regions. With a global CTM (MIROC-ESM-CHEM), we estimated the concentration change of PM_2.5 on the world caused by changes in the emission of a targeted precursor substance from a specific region. Secondly, we used population data and epidemiological concentration response functions (CRFs) of mortality and morbidity to estimate changes in the word’s DALYs occurring due to changes in the concentration of PM_2.5. Finally, the above calculations were done for all ten regions.

Results and discussion

DFs of BCOC, NO _x , and SO₂ for ten regions were estimated. The range of DFs could be up to one order of magnitude among the ten regions in each of the target substances. While population density was an important parameter, variation in transport of PM_2.5 on a continental level occurring due to different emission regions was found to have a significant influence on DFs. Especially for regions of Europe, Russia, and the Middle East, the amount of damage which occurred outside of the emitted region was estimated at a quarter, a quarter, and a third of their DFs, respectively. It was disclosed that the DFs will be underestimated if the transboundary of PM_2.5 is not taken into account in those regions.

Conclusions

The human health damage factors of PM_2.5 produced by BCOC, NO _x , and SO₂ are estimated for ten regions by using one global chemical transport model. It became clear that the variation of transport for PM_2.5 on a continental level greatly influences the regionality in DFs. For further research to quantify regional differences, it is important to consider the regional values of concentration response function (CRF) and DALY loss per case of disease or death.

     

Relationships between nitrogen,dry matter accumulation and glucosinolates in <Emphasis Type="Italic">Eruca sativa</Emphasis> Mills. The applicability of the critical NO<Subscript>3</Subscript>-N levels approach

Background and Aims

Rocket salad (Eruca sativa Mills) is one of the major leafy vegetables produced worldwide and has been characterized as a rich source of chemoprotective glucosinolates (GSL). The relationship between N fertilization and the resulting plant biomass and N status with GSL quantity and quality in rocket leaves was examined.

Methods

A pot experiment was conducted, applying ten different N-rates and destructive sampling was carried out 15, 30 and 45 days after transplanting (DAT). The Mitscherlich equation was used to establish NO₃-N critical levels at each growth stage and as an indicator of N demand for relative maximum dry matter accumulation and glucosinolate content and composition was determined.

Results

Glucosinolate content was significantly influenced by N rate, growth stage and their interaction. Different GSL types showed dissimilar responses to N fertilization: aliphatic GSLs were significantly reduced under increased N rates whereas indole GSL showed the reverse. Under excess N fertilization (>1.04 g/plant), dry matter accumulation remained constant, NO₃-N was significantly increased and total GSL content was significantly reduced, factors that could lead to an anticipated product quality decline.

Conclusions

The application of the critical NO₃-N level approach used to identify optimal N fertilization rates for plant growth could serve as means to obtain optimized GSL content in the edible plant parts.

     

Development of human health damage factors related to CO<Subscript>2</Subscript> emissions by considering future socioeconomic scenarios

Purpose

Global warming is exerting a damaging effect on human health. This damage is not only influenced by future climate conditions but also projected economic development and population growth. That being said, there are no health damage factors related to CO₂ emissions which take into account future socioeconomic scenarios in life cycle impact assessment (LCIA). Thus, the purpose of the current research is to calculate human health damage factors based on the Special Report on Emission Scenarios (SRESs) developed by the Intergovernmental Panel on Climate Change (IPCC).

Methods

The procedure used to calculate the SRES-based damage factors is as follows. First, a framework was developed to calculate damage factors based on multiple parameters: rise in temperature, relative risk increase, mortality rate increase, rise in number of deaths, and disability-adjusted life year (DALY) increase. Secondly, these parameters were calculated for each individual SRES based on the relationship among the parameters and CO₂ emissions, GDP, and population values of each scenario. Finally, the damage factor for each SRES was calculated by multiplying all the parameters that had been calculated based on the CO₂ emission, GDP, and population data in the corresponding scenarios.

Results and discussion

Using this method, the human health damage factors for four SRESs (A1B, A2, B1, and B2) were calculated. The damage factors consisted of six different items: malaria, diarrhea, cardiovascular disease, malnutrition, coastal flooding, and inland flooding. The calculated results by scenario were 2.0?×?10^?7, 6.2?×?10^?7, 2.1?×?10^?7, and 4.2?×?10^?7 DALY/kg CO₂, respectively. The damage caused by malnutrition is the greatest, followed by diarrhea. Regions of Southeast Asia, Africa, and the Middle East showed the highest damages due to their high damage from malnutrition and diarrhea. With regard to the differences among the four damage factors, the difference between the projected future mortality rate and DALY per death based on the future GDP per capita is greater than the difference between the increases in temperature among scenarios dependent on future CO₂ emission.

Conclusions

The human health damage factors related to CO₂ emissions for four SRESs were estimated. As a result of differences between future socioeconomic scenarios, the largest amount of damage per CO₂ emission unit was three times greater than the smallest amount. Therefore, sensitive analysis is highly recommended when seeking to compare damage caused by global warming and other impact categories.

     

The effects of root surface charge and nitrogen forms on the adsorption of aluminum ions by the roots of rice with different aluminum tolerances

Aim

Our objectives were to compare effects of root charge properties on Al adsorption by the roots of rice that differed in Al-tolerance, and to examine effects of different nitrogen forms on charge properties of rice roots and Al adsorption.

Methods

Streaming potential and chemical methods were used to measure root zeta potential and investigate Al chemical forms adsorbed on the roots of rice obtained from solution culture experiments.

Results

Rice roots of the Al-sensitive variety Yangdao-6 carried greater negative charge than the Al-tolerant variety Wuyunjing-7, which meant the roots of Yangdao-6 adsorbed more exchangeable and complexed Al. When both rice varieties were grown in NH₄ ⁺-containing nutrient solutions, there were less functional groups and lower negative surface charge on their roots, which reduced Al adsorption compared to the rice grown in NO₃ ^? containing nutrient solutions. The decline in nutrient solution pH due to NH₄ ⁺ uptake by rice roots was responsible for the reduced numbers of functional groups and the lower negative surface charge on the roots compared to the rice grown in NO₃ ^? containing solutions.

Conclusions

Integrated root surface charge, as expressed by zeta potential, played an important role in Al adsorption by the roots of rice with different Al-tolerance.

     

A colourimetric microplate assay for simple,high throughput assessment of synthetic and biological nitrification inhibitors

Aim

A simple, rapid, colourimetric method for screening biological nitrification inhibitors in plants is presented.

Methods

Our approach combines the use of the Griess assay to track the rate of nitrite (NO₂ ^?) production by pure cultures of ammonia oxidising bacteria in the presence and absence of nitrification inhibitors with a simple method for collecting root exudates from plants. NO₂ ^? formation was tracked colourimetically on a microplate reader over 9 h of incubation. The advantage of this method is that it provides a simple, high throughput means of measuring biological nitrification inhibition in root exudates, using wild-type bacterial cultures.

Results

NO₂ ^? formation rates and inhibition levels measured using the high through-put method were highly correlated with those measured by tracking NO₂ ^? formation using a segmented flow analyser. The method was able to quantify inhibition of Nitrosomonas europaea by the synthetic nitrification inhibitors allythiourea (AT), dicyandiamide (DCD) and 3,4,-dimethylpyrazole phosphate (DMPP) with IC50 values similar to those reported in the literature. The method detected biological nitrification inhibition (BNI) in root exudates from Brachiaria humidicola and the lack of BNI in root exudates from wheat cv. Janz with minimal alteration of the exudates prior to testing. The results also showed that the more common soil ammonia oxidising bacterium (AOB), Nitrosospira multiformis, was much less sensitive to AT and DCD than N. europaea but had similar sensitivity to DMPP.

Conclusions

This method provides a potentially useful way of screening large numbers of root exudate samples allowing for phenotyping of the BNI trait in crop and pasture populations which will be required for the trait to be introduced into commercial varieties.

     

Photochemical ozone creation potentials

Goal, Scope and Background

Photochemical ozone creation potentials (POCPs) typically used in life cycle impact assessment (LCIA) to address the impact category ‘photo-oxidant formation’ only provide factors for particular volatile organic compounds and do not take into account background concentrations and meteorological conditions. However, the formation of ozone from volatile organic compounds (VOCs), carbon monoxide (CO) and nitrogen oxides (NOx) is highly dependent on the background pollutant concentrations and meteorological conditions. Some LCIA manuals therefore recommend working with potentials for high background concentrations of NOx (Derwent 1998), and potentials for low background concentrations of NOx (Andersson-Sköld 1992).

Objectives

This study has introduced an improved set of POCPs independently of meteorological and emission conditions specific to a given period or location. Whereas current POCP values may be relevant to estimate the photo-oxidant formation over a certain (temporally and spatially well-defined) domain, this study has further introduced more relevant values with respect to potential impacts of ozone on human health and environment.

Methods

For the computation of POCP values on the scale of Western Europe, independently of meteorological and emission conditions specific to a given period or location, a Eulerian chemistry-transport numerical model (CHIMERE-continental) has been implemented over three summer seasons. POCPs have been evaluated for ten VOC species (including the whole VOC group), CO and NOx. The coherence of this new set of POCP values with previous studies has been checked. The spatial representa-tivity of POCP values over the simulation domain in Europe has also been addressed. The robustness of these POCP values to changes in the implemented chemical mechanism used in our model has been checked.

Results and Discussion

The POCPs computed in this study were generally lower than the POCPs calculated in previous studies. In the previous studies, but not here, the POCPs have been calculated with particular meteorological conditions (during anti-cyclonic, fair weather conditions) or emission levels (high polluted backgrounds) known to be optimal with respect to ozone formation. Despite the quantitative variations in the POCP values, we have found a good agreement in the relative ranking of the pollutant species between this study and previous studies. It was also shown that POCP values display significant spatial variability over Western Europe (the largest spatial differences were obtained for NO_x where the sign of the POCP value even changes from region to region).

Conclusions

Finally, the temporally and spatially averaged values obtained here for the POCP index update previous values and represent an attempt to generate the most appropriate and accurate scale for European conditions independently of meteorological and emission conditions specific to a given period or location.

Recommendations and Outlook

These new PCOPs should be useful to LCIA-practitioners in further life cycle impact assessment. However, for the NO_x species, we do not recommend the use of the POCP value for LCIA.

     

Fertilization,soil and plant community characteristics determine soil microbial activity in managed temperate grasslands

Background and aims

Contaminated soils can impede germination and growth of selected plant species, restricting effective phytoremediation strategies. The purpose of the present study was to enhance the germination and growth of saltgrass [Distichlis spicata (L.) Greene] by evaluating the efficacy of certain seed pretreatments and soil amendments.

Methods

Ten seed pretreatment methods, two amendments, three soil depths and five saline levels were tested under greenhouse conditions.

Results

Saltgrass germination and growth were negatively correlated with increasing salinity levels when NaCl > 85.6 mM. Among ten seed pretreatments (stratification + Proxy 24 h, hot water + Proxy 24 h, stratification, hot water + Proxy 48 h, Proxy 48 h, Proxy 24 h, hot water, scarification, gibberellins, and KMnO₄), the two best methods were stratification + Proxy 24 h and hot water + Proxy 24 h for enhancing saltgrass germination, with the latter pretreatment being especially useful because of its shorter preparation time and high germination rates. Proxy is a commercial ethephon product. Potting soil (5.0 cm depth) was found to be the best amendment for saltgrass germination and growth in hydrocarbon-contaminated soils.

Conclusion

We conclude that direct seeding of saline soils contaminated with petroleum hydrocarbons is a feasible phytoremediation strategy provided that appropriate seed pretreatments and amendments are utilized.

     

The optimum sevoflurane concentration for supraglottic airway device Blockbuster™ insertion with spontaneous breathing in obese patients: a prospective observational study

Background

Airway management of the obese patient presenting for surgery is more likely to be a challenging problem. Supraglottic airway device has been adopted as a bridge to connect ventilation and tracheal intubation in obese patients who would be suffered with difficult intubation. The optimum sevoflurane concentration for supraglottic airway device insertion allowing spontaneous breathing in 50% of obese patients (ED₅₀) is not known. The purpose of this study was to determine the ED₅₀ of sevoflurane for supraglottic airway device Blockbuster? insertion with spontaneous breathing in obese patients requiring general anesthesia.

Methods

Thirty elective obese patients (body mass index 30-50 kg/m²) undergoing bariatric surgery were recruited in this study. The predetermined target sevoflurane concentration (initiating at 2.5% with 0.5% as a step size) was sustained for >5 min using a modified Dixon’s up-and-down method, and then the supraglottic airway device Blockbuster? was inserted. The patient’s response to supraglottic airway device insertion was classified as either ‘movement’ or ‘no-movement’. The ED₅₀ of sevoflurane were determined by calculating the midpoint concentration of crossover point from ‘movement’ or ‘no-movement’ response.

Results

The ED₅₀ of sevoflurane for supraglottic airway device Blockbuster? insertion in obese patients calculated using up-and-down method were 2.50?±?0.60%. The ED₅₀ and ED₉₅ (95% confidence interval) obtained by probit regression analysis were 2.35 (1.28–3.42) % and 4.03 (3.16–17.83) % for supraglottic airway device Blockbuster? insertion, respectively.

Conclusion

We conclude that the optimum end-tidal sevoflurane concentration required for the supraglottic airway device Blockbuster? insertion allowing spontaneous breathing in 50% of obese patients (ED₅₀) is 2.5?±?0.6%.

Trial registration

Chinese Clinical Trial Registry, ChiCTR-IPR-16009071, Registered on 24 August 2016.

     

Which root traits determine nitrogen uptake by alpine plant species on the Tibetan Plateau?

Background and aims

Nitrogen (N) is one of the most important limiting factors influencing plant growth and reproduction in alpine and tundra ecosystems. However, in situ observations of the effects of root traits on N absorption by alpine plant species are still lacking.

Methods

We investigated the rates of N uptake and the effect of root characteristics in ten common herbaceous alpine plant species using a ¹⁵N isotope tracer technique and the root systems of plants growing in a semi-arid steppe environment on the Tibetan Plateau. Our objective was to determine the root traits (root biomass, volume, surface area, average diameter, length, specific root length and specific root area) that make the largest contribution to the total uptake of N (¹⁵N–NO₃ ^?, ¹⁵N–NH₄ ⁺ or ¹⁵N–glycine) by alpine plant species.

Results

Monocotyledonous species had higher absorption rates for ¹⁵N–NH₄ ⁺, ¹⁵N–NO₃ ^?, ¹⁵N–glycine and total ¹⁵N than dicotyledonous species (P < 0.05). The root biomass, volume, surface area and average diameter were negatively correlated with the absorption capacity for ¹⁵N–NH₄ ⁺, ¹⁵N–NO₃ ^? and total ¹⁵N across the ten alpine plant species. However, the specific root length and the specific root area had significantly positive effects on the uptake of N.

Conclusions

In contrast with traditional views on the uptake of N, the N uptake rate was not improved by a larger root volume or root surface area for these alpine plant species in a high-altitude ecosystem. Root morphological traits had greater impacts on N absorption than traits related to the root system size in alpine herbaceous plants.

     

Pulmonary function,exhaled nitric oxide and symptoms in asthma patients with obesity: a cross-sectional study

Background

Obesity is a risk factor for the development of asthma. In patients with obesity the diagnosis of asthma is often based on symptoms, but without objective measurements. Nevertheless, obesity-associated asthma is recognized as a distinct asthma phenotype. Therefore, this study explores lung function and symptoms in asthma patients with and without obesity.

Methods

The Netherlands Epidemiology of Obesity (NEO) study is a population-based cohort study with 6671 participants (aged 45–65 years) of whom 472 had asthma. Of this latter group, linear regression analysis was used to examine differences in lung function and symptoms between asthma patients with (n?=?248) and without obesity (n?=?224), and between asthma patients with and without increased Fe_NO. Analyses were adjusted for confounders.

Results

Asthma patients with obesity had lower predicted FEV₁ and FVC values than patients without obesity [adjusted mean difference (MD) -3.3% predicted, 95% CI -6.5, ?0.2; adjusted MD ?5.0% predicted, 95% CI -7.8, ?2.1]. The prevalence of symptoms was higher in patients with obesity. Asthma patients with obesity and with increased Fe_NO had lower FEV₁ and FEV₁/FVC values compared with those with low Fe_NO (adjusted MD ?6.9% predicted, 95% CI -11.7, ?2.0; ?2.4%, 95% CI -4.6, ?0.2).

Conclusion

Asthma patients with obesity had lower FEV₁ and FVC values than patients without obesity. This suggests that patients with obesity have restrictive lung function changes, rather than obstructive changes. Asthma patients with obesity and increased Fe_NO showed more obstructive changes. Fe_NO might help to identify patients with eosinophilic inflammation-driven asthma, whereas patients with low Fe_NO might have an obesity-associated asthma phenotype in which symptoms are partly caused by the obesity.

     

Cradle-to-gate life cycle assessment of global manganese alloy production

Purpose

Manganese is a metal used extensively in everyday life, particularly in structural steel. Despite the importance of manganese as an essential alloying element in steel and stainless steel, the environmental profile of manganese alloys lacked globally representative, primary industry data. The International Manganese Institute (IMnI) and Hatch completed the first global life cycle assessment (LCA) of manganese alloy production, providing environmental benchmarks and a firm foundation of accurate data with which to inform other industry-led initiatives.

Methods

The study compiled primary data from 16 ore and alloy producers worldwide, covering 18 % of global ore production and 8 % of global alloy production for 2010. This peer-reviewed, ISO 14040 compliant LCA covers the cradle-to-gate life cycles of silicomanganese, ferromanganese, and refined ferromanganese. The study provides a comprehensive picture of global environmental performance, quantifying energy consumption, global warming potential (GWP), acidification potential (AP), photochemical ozone creation potential (POCP), primary water use, and primary waste generation. A novel model architecture was devised to generate process, site, and cradle-to-gate LCAs for single and multiple sites simultaneously, extracting greater value from the LCA process by facilitating environmental and operational benchmarking within the industry.

Results and discussion

The results of the study show that total GWP, AP, and POCP for 1 kg of average manganese alloy was 6.0 kg CO₂e, 45 g SO₂e, and 3 g C₂H₄e, respectively. Electricity demand and coal and coke consumption during smelting are the dominant operating parameters contributing to environmental performance. On-site air emission measures (GWP, POCP, NO_X, and particulate matter (PM)) contributed 25 to 35 % of total life cycle emissions. Overburden and waste rock were the most significant primary solid waste flows by mass. The study provides a resource for improvement at the global industry and site scales by establishing benchmarks, identifying hotspots, and quantifying the benefits of efficiency savings through process optimization.

Conclusions

This LCA provides accurate primary data to improve steel and stainless steel product LCAs and communicate the environmental performance of the industry in quantitative terms. It facilitates dialogue between manganese producers and consumers through a shared understanding of the environmental profile of the industry. Through leveraging the study to identify hotspots within the manganese supply chain, producers can work both independently and collectively towards improving the environmental and economic performance of manganese alloys.

     

Proteomics and multivariate modelling reveal sex-specific alterations in distinct regions of human carotid atheroma

Background

Atherosclerotic lesions are comprised of distinct regions with different proteomic profiles. Men and women develop differences in lesion phenotype, with lesions from women generally being more stable and less prone to rupture. We aimed to investigate the differences in proteomic profiles between sexes, including distinct lesion regions, to identify altered proteins that contribute to these differences observed clinically.

Methods

Carotid endarterectomy samples (ten men/ten women) were obtained, and intraplaque biopsies from three distinct regions (internal control, fatty streak and plaque) were analysed by tandem-mass spectrometry. Multivariate statistical modelling, using orthogonal partial least square-discriminant analysis, was used to discriminate the proteomes between men and women.

Results

Multivariate discriminant modelling revealed proteins from 16 functional groups that displayed sex-specific associations. Additional statistics revealed ten proteins that display region-specific alterations when comparing sexes, including proteins related to inflammatory response, response to reactive oxygen species, complement activation, transport and blood coagulation. Transport protein afamin and blood coagulation proteins antithrombin-III and coagulation factor XII were significantly increased in plaque region from women. Inflammatory response proteins lysozyme C and phospholipase A2 membrane-associated were significantly increased in plaque region from men. Limitations with this study are the small sample size, limited patient information and lack of complementary histology to control for cell type differences between sexes.

Conclusions

This pilot study, for the first time, utilises a multivariate proteomic approach to investigate sexual dimorphism in human atherosclerotic tissue, and provides an essential proteomic platform for further investigations to help understand sexual dimorphism and plaque vulnerability in atherosclerosis.

     

Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics

Introduction

Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites.

Objectives

In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting.

Methods

The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces.

Results

A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed.

Conclusion

The workflow generated repeatable and informative fingerprints for robust metabolome characterization.

     

Evaluation of cervical posture improvement of children with cerebral palsy after physical therapy based on head movements and serious games

Background

This paper presents the preliminary results of a novel rehabilitation therapy for cervical and trunk control of children with cerebral palsy (CP) based on serious videogames and physical exercise.

Materials

The therapy is based on the use of the ENLAZA Interface, a head mouse based on inertial technology that will be used to control a set of serious videogames with movements of the head.

Methods

Ten users with CP participated in the study. Whereas the control group (n = 5) followed traditional therapies, the experimental group (n = 5) complemented these therapies with a series of ten sessions of gaming with ENLAZA to exercise cervical flexion–extensions, rotations and inclinations in a controlled, engaging environment.

Results

The ten work sessions yielded improvements in head and trunk control that were higher in the experimental group for Visual Analogue Scale, Goal Attainment Scaling and Trunk Control Measurement Scale (TCMS). Significant differences (27% vs. 2% of percentage improvement) were found between the experimental and control groups for TCMS (p < 0.05). The kinematic assessment shows that there were some improvements in the active and the passive range of motion. However, no significant differences were found pre- and post-intervention.

Conclusions

Physical therapy that combines serious games with traditional rehabilitation could allow children with CP to achieve larger function improvements in the trunk and cervical regions. However, given the limited scope of this trial (n = 10) additional studies are needed to corroborate this hypothesis.

     

Corneal Collagen Cross-Linking for the Management of Mycotic Keratitis

Purpose

To evaluate the efficiency of corneal collagen cross-linking (CXL) in addition to topical voriconazole in cases with mycotic keratitis.

Design

Retrospective case series in a tertiary university hospital.

Participants

CXL was performed on 13 patients with mycotic keratitis who presented poor or no response to topical voriconazole treatment.

Methods

The clinical features, symptoms, treatment results and complications were recorded retrospectively. The corneal infection was graded according to the depth of infection into the stroma (from grade 1 to grade 3). The visual analogue scale was used to calculate the pain score before and 2 days after surgery.

Main Outcome Measures

Grade of the corneal infection.

Results

Mean age of 13 patients (6 female and 7 male) was 42.4 ± 17.7 years (20–74 years). Fungus was demonstrated in culture (eight patients) or cytological examination (five patients). Seven of the 13 patients (54%) were healed with topical voriconazole and CXL adjuvant treatment in 26 ± 10 days (15–40 days). The remaining six patients did not respond to CXL treatment; they initially presented with higher grade ulcers. Pre- and post-operative pain score values were 8 ± 0.8 and 3.5 ± 1, respectively (p < 0.05).

Conclusions

The current study suggests that adjunctive CXL treatment is effective in patients with small and superficial mycotic ulcers. These observations require further research by large randomized clinical trials.

     

Catesbeianin-1, a novel antimicrobial peptide isolated from the skin of <Emphasis Type="Italic">Lithobates catesbeianus</Emphasis> (American bullfrog)

Objectives

To identify and characterize a novel antimicrobial peptide, catesbeianin-1.

Results

Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 exhibited moderate hemolytic activity (approx 8%) at 100 μg/ml, and its HC₅₀ (50% hemolytic concentration) was 300 μg/ml. Its cytotoxicity was approx 10–20% at 100 μg/ml, and its CC₅₀ (50% cytotoxic concentration) was >100 μg/ml. The LD₅₀ of catesbeianin-1 in mice was 80 mg/kg. At 3.1 µg/ml, catesbeianin-1 significantly inhibited the growth of methicillin-resistant Staphylococcus aureus.

Conclusions

A new antimicrobial peptide from the skin of Lithobates catesbeianus (American bullfrog) may represent a template for the development of novel antimicrobial agents.

     

Immobilization of lactoperoxidase on graphene oxide nanosheets with improved activity and stability

Objectives

The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS).

Methods

Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using glutaraldehyde as cross-linker. Kinetic properties and stability of free and immobilized LPO were investigated.

Results

LPO was purified 59.13 fold with a specific activity of 5.78 U/mg protein. The successful immobilization of LPO on functionalized GO-NS was confirmed by using dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FT-IR). The overall results showed that the stability of the immobilized LPO was considerably improved compared to free LPO. Apparent K_m and V_max of LPO also indicated that the immobilized enzyme had greater affinity to the substrate than the native enzyme.

Conclusions

Graphene oxide nanosheets are effective means for immobilization of LPO.

     

Intensive determination of storage condition effects on human plasma metabolomics

Introduction

Human plasma metabolomics offer powerful tools for understanding disease mechanisms and identifying clinical biomarkers for diagnosis, efficacy prediction and patient stratification. Although storage conditions can affect the reliability of data from metabolites, strict control of these conditions remains challenging, particularly when clinical samples are included from multiple centers. Therefore, it is necessary to consider stability profiles of each analyte.

Objectives

The purpose of this study was to extract unstable metabolites from vast metabolome data and identify factors that cause instability.

Method

Plasma samples were obtained from five healthy volunteers, were stored under ten different conditions of time and temperature and were quantified using leading-edge metabolomics. Instability was evaluated by comparing quantitation values under each storage condition with those obtained after ?80 °C storage.

Result

Stability profiling of the 992 metabolites showed time- and temperature-dependent increases in numbers of significantly changed metabolites. This large volume of data enabled comparisons of unstable metabolites with their related molecules and allowed identification of causative factors, including compound-specific enzymatic activity in plasma and chemical reactivity. Furthermore, these analyses indicated extreme instability of 1-docosahexaenoylglycerol, 1-arachidonoylglycerophosphate, cystine, cysteine and N⁶-methyladenosine.

Conclusion

A large volume of data regarding storage stability was obtained. These data are a contribution to the discovery of biomarker candidates without misselection based on unreliable values and to the establishment of suitable handling procedures for targeted biomarker quantification.

     

Harvesting <Emphasis Type="Italic">Chlorella vulgaris</Emphasis> via rapid sedimentation induced by combined coagulants and tapered shear

Objectives

In this study, a rapid sedimentation induced by combined coagulants and gradual shear was developed to harvest Chlorella vulgaris.

Results

The microalgal harvesting efficiency was observably promoted by the synergistic effect between FeCl₃ and PAM, especially in the first 10 min. A higher harvesting efficiency, 95.61%, could be achieved within approximately 3 min due to the large and dense flocs generated by the combined coagulants. In contrast, the efficiencies were only 54.25 and 60.20% with FeCl₃ and PAM, independently. When coagulation was performed under gradually reduced shear (from 50 to 30 rpm), smaller clusters or cells filled the pores of the aggregates via interception, which caused the flocs to become larger and more compact.

Conclusions

The sedimentation time was shortened to 30 s for microalgal coagulation induced by the simultaneous use of combined coagulants and tapered shear, providing an effective approach to harvesting microalgae.