Comparative LCA of recycled and conventional concrete for structural applications

Purpose

Construction and demolition (C&D) waste recycling has been considered to be a valuable option not only for minimising C&D waste streams to landfills but also for mitigating primary mineral resource depletion. However, the potentially higher cement demand due to the larger surface of the coarse recycled aggregates challenges the environmental benefits of recycling concrete. Furthermore, it is unclear how the environmental impacts depend on concrete mixture, cement type, aggregates composition and transport distances.

Methods

We therefore analysed the life cycle impacts of 12 recycled concrete (RC) mixtures with two different cement types and compared it with corresponding conventional concretes (CC) for three structural applications. The RC mixtures were selected according to laws, standards and construction practice in Switzerland. We compared the environmental impacts of ready-for-use concrete on the construction site, assuming equal lifetimes for recycled and conventional concrete in a full life cycle assessment. System expansion and substitution are considered to achieve the same functionality for all systems.

Results and discussion

The results show clear (～30 %) environmental benefits for all RC options at endpoint level (ecoindicator 99 and ecological scarcity). The difference is mainly due to the avoided burdens associated to reinforcing steel recycling and avoided disposal of C&D waste. Regarding global warming potential (GWP), the results are more balanced and primarily depend on the additional amount of cement needed for RC. Above 22 to 40 kg additional cement per cubic metre of concrete, RC exhibits a GWP comparable to CC. Additional transport distances above 15 km for the RC options do result in environmental impacts higher than those for CC.

Conclusions

In summary, the current market mixtures of recycled concrete in Switzerland show significant environmental benefits compared to conventional concrete and cause similar GWP, if additional cement and transport for RC are limited.

     

Life cycle environmental assessment of industrial hazardous waste incineration and landfilling in China

Purpose

The improper handling of industrial hazardous waste (IHW), which comprises large amounts of toxic chemicals, heavy metals, or irradiation substances, is a considerable threat to human health and the environment. This study aims to quantify the life cycle environmental impacts of IHW landfilling and incineration in China, to identify its key factors, to improve its potential effects, and to establish a hazardous waste disposal inventory.

Methods

Life cycle assessment was conducted using the ReCiPe model to estimate the environmental impact of IHW landfilling and incineration. The characterization factors for the human toxicity and freshwater ecotoxicity categories shown in the ReCiPe were updated based on the geographies, population, food intake, and environmental conditions in China.

Results and discussion

The overall environmental burden was mainly attributed to the carcinogen category. The national carcinogen burden in 2014 at 37.8 CTUh was dominated by diesel consumption, cement and sodium hydroxide production, direct emission, transportation, and electricity generation stages caused by direct mercury and arsenic emissions, as well as indirect chromium emission. Although the atmospheric mercury emission directly caused by IHW incineration was comparative with the emission levels of developed countries, the annual direct mercury emission accounted for approximately 0.1% of the national mercury emission.

Conclusions

The key factors contributing to the reduction of the national environmental burden include the increasing diesel and electricity consumption efficiency, the reduction of cement and sodium hydroxide use, the development of air pollutant controlling systems, the reduction of transport distance between IHW disposers to suppliers, and the improvement of IHW recycling and reuse technologies.

     

A life cycle comparison of disposal and beneficial use of coal combustion products in Florida

Background, Goal, and Scope

Currently, only 40%, or 44.5 million metric tons, of coal combustion products (CCPs) generated in the United States each year by electric utilities are diverted from disposal in landfills or surface impoundments and recycled. Despite promising economic and environmental savings, there has been scant attention devoted to assessing life cycle impacts of CCP disposal and beneficial use. The objective of this paper is to present a life cycle inventory considering two cases of CCP management, including the stages of coal mining and preparation, coal combustion, CCP disposal, and CCP beneficial use. Six beneficial uses were considered: concrete production, structural fills, soil amendments, road construction, blasting grit and roofing granules, and wallboard.

Methods

Primary data for raw material inputs and emissions of all stages considered were obtained from surveys and site visits of coal-burning utilities in Florida conducted in 2002, and secondary data were obtained from various published sources and from databases available in SimaPro 5.1 (PRé Consultants, Amersfoort, The Netherlands).

Results

Results revealed that 50 percent of all CCPs produced, or 108 kg per 1,000 kg of coal combusted, are diverted for application in a beneficial use; however, the relative amounts sold by each utility is dependent on the process operating parameters, air emission control devices, and resulting quality of CCP. Diversion of 50% of all CCPs to beneficial use applications yields a decrease in the total raw materials requirements (with the exception of gravel and iron) and most emissions to air, water, and land, as compared to 100% disposal.

Discussion

The greatest reduction of raw materials was attributed to replacing Portland cement with fly ash, using bottom ash as an aggregate in concrete production and road construction in place of natural materials, and substituting FGD gypsum for natural gypsum in wallboard. The use of fly ash as cementitious material in concrete also promised significant reductions in emissions, particularly the carbon dioxide that would be generated from Portland cement production. Beneficial uses of fly ash and gypsum showed reductions of emissions to water (particularly total dissolved solids) and emissions of metals to land, although these reductions were small compared to simply diverting 50% of all CCPs from landfills or surface impoundments.

Conclusions

This life cycle inventory (LCI) provides the foundation for assessing the impacts of CCP disposal and beneficial use. Beneficial use of CCPs is shown here to yield reductions in raw material requirements and various emissions to all environmental compartments, with potential tangible savings to human health and the environment.

Recommendations and Perspectives

Extension of this life cycle inventory to include impact assessment and sensitivity analysis will enable a determination of whether the savings in emissions reported here actually result in significant improvements in environmental and human health impacts.

     

Comparative life-cycle impact assessment of concrete manufacturing in Singapore

Purpose

For countries like Singapore that is highly dependent on imported goods, it is essential to consider the consequences of consumption of imported cement and other concrete constituents for a fair carbon trading at global and regional levels. Recently, as a result of reduction in trade barriers and costs of materials and fuels, Singapore does not have much incentive in reducing environmental impacts of these imported goods. However, Singapore has set high environmental targets nationally to reduce impacts from building and construction. In addition to its national efforts, Singapore also needs to take action in trade-related consequences of importing energy-intensive products like cement and aggregates to Singapore. The purpose of this study is to quantify and suggest alternatives for reducing the embodied energy and life-cycle impacts of concrete consumption in Singapore on the basis of current trading volumes of these materials from Singapore’s importers.

Methods

A detailed life-cycle assessment of concrete manufacturing in Singapore is performed to suggest possible ways to reduce the environmental impacts from importing cement and aggregates from Singapore’s trade partners based on an earlier life-cycle inventory developed for Singapore and its neighboring countries. Life-cycle impact assessment (LCIA) impact characterization factors are based on a midpoint-oriented and hierarchist approach as defined by ReCiPe method. Following the LCIA, a scenario analysis is conducted to select the best combination of cement and aggregate importers of Singapore based on their environmental performance.

Results and discussion

Results from the scenario analysis show that overall impacts can be reduced by importing the materials from a nearer source with efficient production technologies and greener fuel mixes. About 10–34 % reduction is estimated in embodied energy, acidification, eutrophication, global warming potential, smog, and health impacts by importing from a closer and technologically greener source.

Conclusions

Despite the limitations due to data and modeling uncertainties, this study constitutes a baseline/benchmark for addressing the current cement and aggregate markets and associated environmental impacts of concrete consumption in Singapore based on historical import quantities of cement and aggregates from neighboring countries of Singapore. In the near future, policy-related action would be influential in achieving Singapore’s national and global environmental targets in buildings and construction sector. Incorporation of an LCA approach into Green Mark Scheme (GMS) by the Building and Construction Authority (BCA) is recommended for Singapore to comply both with its national goals and with its new climate action plan to the UN Framework Convention on Climate Change.

     

Metabolomic profiling of maternal hair suggests rapid development of intrahepatic cholestasis of pregnancy

Introduction

Intrahepatic cholestasis of pregnancy (ICP) is a common maternal liver disease; development can result in devastating consequences, including sudden fetal death and stillbirth. Currently, recognition of ICP only occurs following onset of clinical symptoms.

Objective

Investigate the maternal hair metabolome for predictive biomarkers of ICP.

Methods

The maternal hair metabolome (gestational age of sampling between 17 and 41 weeks) of 38 Chinese women with ICP and 46 pregnant controls was analysed using gas chromatography–mass spectrometry.

Results

Of 105 metabolites detected in hair, none were significantly associated with ICP.

Conclusion

Hair samples represent accumulative environmental exposure over time. Samples collected at the onset of ICP did not reveal any metabolic shifts, suggesting rapid development of the disease.

     

LCA and LCC of the world’s longest pier: a case study on nickel-containing stainless steel rebar

Purpose

Built in 1941, the Progreso Pier was the first concrete structure in the world built with nickel-containing stainless steel reinforcement. The Pier has been in service for over 70 years without any significant repair or maintenance activities. The aim of this study was to understand the environmental and economic implications of selecting nickel-containing stainless steel reinforcement using the Progreso Pier as the case study.

Methods

A combined environmental life cycle assessment (LCA) and life cycle costing (LCC) study was conducted. The analysis considered the potential environmental impacts and the net present cost of the stainless steel reinforced structure from cradle to grave and compared it to the same structure using conventional carbon steel.

Results and discussion

The results indicated that while using stainless steel reinforcement resulted in a marginally higher environmental impact after initial construction, this is offset by the increased service life and, hence, less frequent maintenance and reconstruction activities. Relative to the as-built stainless steel reinforcement design, the environmental impacts of the carbon steel reinforced design are between 69 and 79 % higher over the analysis period. Similar observations were made for the other investigated impact categories. The cost implications of using stainless steel reinforcement show economic benefits that are complementary to the environmental benefits. Similar to the LCA, the service life benefits outweigh the higher unit costs for stainless steel, assuming a discount rate of 0.01 % as the baseline scenario. The carbon steel reinforced design has a net present cost that is 44 % higher than the as-built stainless steel reinforcement design. The crossover point for the two designs occurs at year 50, which corresponds to the reconstruction activity. A sensitivity analysis shows that the results and conclusions are sensitive to the choice in discount rate: Rates 3 % and lower produce net present costs that are lower for the as-built design; rates 4 % and higher produce net present costs that are lower for the alternative design.

Conclusions

The study demonstrates how LCA and LCC are complementary tools that can be used in decision-making for sustainable construction. The Progreso Pier exemplifies the importance of considering the entire life cycle with service life and recycling as well as long-term life cycle impacts of infrastructure projects from an environmental and economic perspective.

     

Life cycle assessment of copper production: a case study in China

Purpose

China is the world’s largest producer and consumer of refined and reclaimed copper because of the rapid economic and industrial development of this country. However, only a few studies have analyzed the environmental impact of China’s copper industry. The current study analyzes the life cycle environmental impact of copper production in China.

Methods

A life cycle impact assessment using the ReCiPe method was conducted to estimate the environmental impact of refined and reclaimed copper production in China. Uncertainty analysis was also performed based on the Monte-Carlo simulation.

Results and discussion

The environmental impact of refined copper was higher than that of reclaimed copper in almost all categories except for human toxicity because of the direct atmospheric arsenic emission during the copper recycling stage. The overall environmental impact for the refined copper production was mainly attributed to metal depletion, freshwater ecotoxicity, marine ecotoxicity, and water depletion potential impact. By contrast, that for the reclaimed copper production was mainly caused by human toxicity impact.

Conclusions

Results show that the reclaimed copper scenario had approximately 59 to 99% more environmental benefits than those of the refined copper scenario in most key categories except for human toxicity, in which a similar environmental burden was observed between both scenarios. The key factors that reduce the overall environmental impact for China’s copper industry include decreasing direct heavy metal emissions in air and water, increasing the national recycling rate of copper, improving electricity consumption efficiency, replacing coal with clean energy sources for electricity production, and optimizing the efficiency of copper ore mining and consumption.

     

Uncovering the metabolic response of abalone (<Emphasis Type="Italic">Haliotis midae)</Emphasis> to environmental hypoxia through metabolomics

Introduction

Oxygen is essential for metabolic processes and in the absence thereof alternative metabolic pathways are required for energy production, as seen in marine invertebrates like abalone. Even though hypoxia has been responsible for significant losses to the aquaculture industry, the overall metabolic adaptations of abalone in response to environmental hypoxia are as yet, not fully elucidated.

Objective

To use a multiplatform metabolomics approach to characterize the metabolic changes associated with energy production in abalone (Haliotis midae) when exposed to environmental hypoxia.

Methods

Metabolomics analysis of abalone adductor and foot muscle, left and right gill, hemolymph, and epipodial tissue samples were conducted using a multiplatform approach, which included untargeted NMR spectroscopy, untargeted and targeted LC–MS spectrometry, and untargeted and semi-targeted GC-MS spectrometric analyses.

Results

Increased levels of anaerobic end-products specific to marine animals were found which include alanopine, strombine, tauropine and octopine. These were accompanied by elevated lactate, succinate and arginine, of which the latter is a product of phosphoarginine breakdown in abalone. Primarily amino acid metabolism was affected, with carbohydrate and lipid metabolism assisting with anaerobic energy production to a lesser extent. Different tissues showed varied metabolic responses to hypoxia, with the largest metabolic changes in the adductor muscle.

Conclusions

From this investigation, it becomes evident that abalone have well-developed (yet understudied) metabolic mechanisms for surviving hypoxic periods. Furthermore, metabolomics serves as a powerful tool for investigating the altered metabolic processes in abalone.

     

Metabolic response of porcine colon explants to in vitro infection by <Emphasis Type="Italic">Brachyspira hyodysenteriae</Emphasis>: a leap into disease pathophysiology

Introduction

Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available.

Objective

The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae.

Methods

Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes.

Results

Significant epithelial necrosis, increased levels of l-citrulline and IL-1α were observed on explants infected with B. hyodysenteriae.

Conclusions

The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1α and NO.

     

Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics

Introduction

Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites.

Objectives

In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting.

Methods

The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces.

Results

A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed.

Conclusion

The workflow generated repeatable and informative fingerprints for robust metabolome characterization.

     

Life cycle assessment-based selection of a sustainable lightweight automotive engine hood design

Purpose

This study presents a life cycle assessment (LCA)-based sustainable and lightweight automotive engine hood design and compares the life cycle energy consumption and potential environmental impacts of a steel (baseline) automotive engine hood with three types of lightweight design: advanced high strength steel (AHSS), aluminum, and carbon fiber.

Methods

A “cradle-to-grave” LCA including the production, use, and end-of-life stages is conducted in accordance with the ISO 14040/14044 standards. Onsite data collected by Chinese automotive companies in 2015 are used in the assessment. The Cumulative Energy Demand v1.09 method is applied to evaluate cumulative energy demand (CED), and the International Panel on Climate Change 2013 100a method is used to estimate global warming potential (GWP 100a).

Results and discussion

Among the different lightweight designs for the engine hood, the aluminum design is the most sustainable and has the lowest CED and GWP (100a) from a life cycle perspective, which is based on a lifetime driving distance of approximately 150,000 km. In addition, the AHSS design is also sustainable and lightweight. The carbon fiber design results in higher CED and GWP (100a) values than the steel (baseline) design during the life cycle but results in the largest CED and GWP (100a) savings through waste material recycling. The AHSS design exhibits the best break-even distance based on CED and GWP (100a) within 150,000 km.

Conclusions

Sensitivity analysis results show that the lifetime driving distance and material recycling rate have the largest impacts on the overall CEDs and GWPs of the three lightweight designs.

     

A modeling framework to evaluate sustainability of building construction based on LCSA

Purpose

Life cycle sustainability assessment (LCSA) is a method that combines three life cycle techniques, viz. environmental life cycle assessment (LCA), life cycle costing (LCC), and social life cycle assessment (S-LCA). This study is intended to develop a LCSA framework and a case study of LCSA for building construction projects.

Methods

A LCSA framework is proposed to combine the three life cycle techniques. In the modeling phases, three life cycle models are used in the LCSA framework, namely the environmental model of construction (EMoC), cost model of construction (CMoC), and social-impact model of construction (SMoC). A residential building project is applied to the proposed LCSA framework from “cradle to the end of construction” processes to unveil the limitations and future research needs of the LCSA framework.

Results and discussion

It is found that material extraction and manufacturing account for over 90 % to the environmental impacts while they contribute to 61 % to the construction cost. In terms of social impacts, on-site construction performs better than material extraction and manufacturing, and on-site construction has larger contributions to the positive social impacts. The model outcomes are validated through interviews with local experts in Hong Kong. The result indicates that the performance of the models is generally satisfactory.

Conclusions

The case study has confirmed that LCSA is feasible. Being one of the first applications of LCSA on building construction, this study fulfills the current research gap and paves the way for future development of LCSA.

     

Citrate usage in the leading causes of blindness: new possibilities for the old metabolite

Introduction

Citrate is an old metabolite which is best known for the role in the Krebs cycle. Citrate is widely used in many branches of medicine. In ophthalmology citrate is considered as a therapeutic agent and an useful diagnostic tool—biomarker.

Objectives

To summarize the published literature on citrate usage in the leading causes of blindness and highlight the new possibilities for this old metabolite.

Methods

We conducted a systematic search of the scientific literature about citrate usage in ophthalmology up to January 2018. The reference lists of identified articles were searched for providing in-depth information.

Results

This systematic review included 30 articles. The role of citrate in the leading causes of blindness is presented.

Conclusions

Citrate might help inhibit cataract progression, in case of questions confirm glaucoma diagnosis or improve cornea repair treatment as adjuvant agent (therapy of ulcerating cornea after alkali injury, crosslinking procedure). However, the knowledge about possible citrate usage in ophthalmology is not widely known. Promoting recent scientific knowledge about citrate usage in ophthalmology may not only benefit of medical improvement but may also limit economic costs caused by leading causes of blindness. Further studies on citrate usage in ophthalmology should continuously be the field of scientific interest.

     

Environmental performance of social housing in emerging economies: life cycle assessment of conventional and alternative construction methods in the Philippines

Purpose

The environmental impact of the social building stock is relevant, particularly in emerging economies. Life cycle thinking is not yet established, however. Locally available, alternative building concepts could potentially reduce the environmental impact of the construction segment. This paper examines the environmental performance of “as-built” low-cost housing for an example of the Philippines, and the potential to reduce its environmental impact through use of three alternative building technologies: cement–bamboo frames, soil–cement blocks, and coconut board-based housing.

Methods

Life cycle assessment models are implemented and evaluated with software SimaPro, using the single-impact indicators global warming potential (GWP) and cumulative energy demand (CED) and the multi-impact indicator Impact2002+. According to EN 15978, the life cycle phase product and construction process (A), use stage (B), end-of-life (C) and supplementary information beyond the building life cycle (D) have been assessed. Theoretically calculated inflows from standard construction procedures used in phase A have been verified with 3 years of empirical data from implemented construction projects. For phases B, C and D, attention was given to service life, use-phase, allocation of waste products, biogenic carbon and land-use assumptions. Scenarios reflect the actual situation in the emerging economy. Processes, such as heat recovery from thermal utilization, which are not existing nor near to implementation, were excluded.

Results and discussion

For an assessment of the phases A–B–C–D with GWP, a 35% reduction of environmental impact for soil–cement blocks, 74% for cement–bamboo frame, and 83% for coconut board-based houses is obtained relative to a concrete reference house. In absolute terms, this relates to a reduction of 4.4, 9.3, and 10.3 t CO₂ equivalents over a service life of 25 years. CED showed higher impacts for the biogenic construction methods coconut board and cement–bamboo frames of +8.0 and +4.7%, while the soil–cement technology was evaluated ?7.1% compared to GWP. Sixteen of 17 midpoint categories of Impact2002+ confirmed an overall reduction potential of the alternative building methods, with the midpoint category land occupation being the exception rating the conventional practice over the alternatives.

Conclusions

It is concluded that the alternative construction technologies have substantial potential to reduce the environmental burden caused by the social housing sector. The service life of the alternative technologies plays a vital role for it. LCA for emerging economies needs to incorporate realistic scenarios applicable at their current state or belonging to the most probable alternatives to ensure valuable results. Recommendations for further research are provided.

     

Steel’s recyclability: demonstrating the benefits of recycling steel to achieve a circular economy

Purpose

In a world where the population is expected to peak at around 9 billion people in the next 30 to 40 years, carefully managing our finite natural resources is becoming critical. We must abandon the outdated ‘take, make, consume and dispose’ mentality and move toward a circular economy model for optimal resource efficiency. Products must be designed for reuse and remanufacturing, which would reduce significant costs in terms of energy and natural resources.

Methods

To measure progress in achieving a circular economy, we need a life cycle approach that measures the social, economic and environmental impact of a product throughout its full life cycle—from raw material extraction to end-of-life (EoL) recycling or disposal. Life cycle thinking must become a key requirement for all manufacturing decisions, ensuring that the most appropriate material is chosen for the specific application, considering all aspects of a products’ life. The steel industry has been developing LCI data for 20 years. This is used to assess a product’s environmental performance from steel production to steel recycling at end-of-life. The steel industry has developed a methodology to show the benefits of using recycled steel to make new products. Using recycled materials also carries an embodied burden that should be considered when undertaking a full LCA.

Results and discussion

The recycling methodology is in accordance with ISO 14040/44:2006 and considers the environmental burden of using steel scrap and the benefit of scrap recycling from end-of-life products. It considers the recycling of scrap into new steel as closed material loop recycling, and thus, recycling steel scrap avoids the production of primary steel. The methodology developed shows that for every 1 kg of steel scrap that is recycled at the end of the products life, a saving of 1.5 kg CO₂-e emissions, 13.4 MJ primary energy and 1.4 kg iron ore can be achieved. This equates to 73, 64 and 90 %, respectively, when compared to 100 % primary production.

Conclusions

Incorporating this recycling methodology into a full LCA demonstrates how the steel industry is an integral part of the circular economy model which promotes zero waste; a reduction in the amount of materials used and encourages the reuse and recycling of materials.

     

Global proteomic mapping of alkali stress regulated molecular networks in <Emphasis Type="Italic">Helianthus tuberosus</Emphasis> L.

Background and aims

Soil salinization with high pH condition is a major abiotic stress to plant growth and crop productivity. Helianthus tuberosus L. is an important stress tolerant plant and can survive in the saline-alkali soil and semiarid areas. The aim of this study is to identify the effect of alkali stress on H. tuberosus through global proteomics analysis and improve understanding of the alkalinity resistance of plants.

Methods

H. tuberosus seedlings were exposed to different level alkali stress for 7 days. Protein profiling was quantified by conducting MS-based comparative proteomics analysis. RT-PCR study was carried out to analyze the mRNA expression levels of candidate alkali stress response proteins.

Results

The response of H. tuberosus to alkali stress was detected at both physiological and molecular levels. 104 differentially expressed proteins from H. tuberosus leaves response to Na₂CO₃ treatment were successfully identified. Functional categorization of these identified proteins showed that the accumulation level of proteins involved in glycolysis, TCA cycle, PSI system, ROS scavenging and signal transduction increased under alkali stress.

Conclusions

Based on the observation of plant growth and the investigation of molecular regulation, H.tuberosus could resist certain alkali stress by modulating carbohydrate metabolism and redox homeostasis. These findings provide a new sight into the underlying molecular mechanisms of alkali resistance in plant.

     

A lost opportunity for science: journals promote data sharing in metabolomics but do not enforce it

Introduction

Data sharing is being increasingly required by journals and has been heralded as a solution to the ‘replication crisis’.

Objectives

(i) Review data sharing policies of journals publishing the most metabolomics papers associated with open data and (ii) compare these journals’ policies to those that publish the most metabolomics papers.

Methods

A PubMed search was used to identify metabolomics papers. Metabolomics data repositories were manually searched for linked publications.

Results

Journals that support data sharing are not necessarily those with the most papers associated to open metabolomics data.

Conclusion

Further efforts are required to improve data sharing in metabolomics.

     

Media optimization for extracellular amylase production by <Emphasis Type="Italic">Pseudomonas balearica</Emphasis> vitps19 using response surface methodology

Background

In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS19 isolated from agricultural lands in Kolathur, India.

Methods

Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors–maltose (C-source), malt extract (Nsource), pH, and CaCl₂ was studied.

Results

The factors pH and CaCl₂ concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production.

Conclusion

Amylase production was thus optimized and increased yield was achieved.

     

Lightweighting in light commercial vehicles: cradle-to-grave life cycle assessment of a safety-relevant component

Purpose

Currently, the reduction of weight in automotive is a very important topic in order to lower the air pollution. In this context, the purpose of the present paper was to analyze a real case study through a comparison of the environmental sustainability between a conventional steel crossbeam for light commercial vehicles and an innovative lightweight aluminum one.

Methods

For both scenarios, a cradle-to-grave life cycle assessment methodology and a sensitivity analysis has been used through the study of the following phases: mineral extraction, component manufacturing, use on vehicle, and end of life. In particular, many primary data and a complete vehicle model simulation with three different European driving cycles have been used in order to reach the highest possible level of accuracy during the analysis.

Results and discussion

Regarding the manufacturing phase, the aluminum component’s production gave the highest impact because of the high energy required in the mineral reduction. Anyway, this stage of the analysis had a low effect on the entire LCA, because the benefit of weight reduction during vehicle use showed a strongly higher contribution. The urban driving cycle had the most relevant impact, as a consequence of the frequent start and stop operations and the longest time with engine at idle speed, while the extra-urban cycle is the less demanding due to its higher average speed and no start and stop.

Conclusions

In conclusion, the present research demonstrated the environmental importance of the lightweight for an actual case study in the commercial vehicles field.

     

Pseudo current density maps of electrophysiological heart,nerve or brain function and their physical basis

Background

In recent years the visualization of biomagnetic measurement data by so-called pseudo current density maps or Hosaka-Cohen (HC) transformations became popular.

Methods

The physical basis of these intuitive maps is clarified by means of analytically solvable problems.

Results

Examples in magnetocardiography, magnetoencephalography and magnetoneurography demonstrate the usefulness of this method.

Conclusion

Hardware realizations of the HC-transformation and some similar transformations are discussed which could advantageously support cross-platform comparability of biomagnetic measurements.