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1.

Purpose

Variability in consumer behaviour can significantly influence the environmental performance of products and their associated impacts and this is typically not quantified in life cycle assessments. The goal of this paper is to demonstrate how consumer behaviour data can be used to understand and quantify the variability in the greenhouse gas emissions from domestic laundry washing across Europe.

Methods

Data from a pan-European consumer survey of product usage and washing habits was combined with internal company data on product format greenhouse gas (GHG) footprints and in-home measurement of energy consumption of laundry washing as well as literature data to determine the GHG footprint of laundry washing. The variability associated with four laundry detergent product formats and four wash temperature settings in washing machines were quantified on a per wash cycle basis across 23 European countries. The variability in GHG emissions associated with country electricity grid mixes was also taken into account. Monte Carlo methods were used to convert the variability in the input parameters into variability of the life cycle GHG emissions. Rank correlation analysis was used to quantify the importance of the different sources of variability.

Results and discussion

Both inter-country differences in background electricity mix as well as intra-country variation in consumer behaviour are important for determining the variability in life cycle GHG emissions of laundry detergents. The average GHG emissions related to the laundry washing process in the 23 European countries in 2014 was estimated to be 5?×?102 g CO2?eq/wash cycle, but varied by a factor of 6.5 between countries. Intra-country variability is between a factor of 3.5 and 5.0 (90% interval). For countries with a mainly fossil-based electricity system, the dominant source of variability in GHG emissions results from consumer choices in the use of washing machines. For countries with a relatively low-carbon electricity mix, variability in life cycle GHG emissions is mainly determined by laundry product-related parameters.

Conclusions

The combination of rich data sources enabled the quantification of the variability in the life cycle GHG emissions of laundry washing which is driven by a variety of consumer choices, manufacturer choices and infrastructural differences of countries. The improved understanding of the variability needs to be balanced against the cost and challenges of assessing of consumer habits.
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2.
3.

Purpose

The well-to-wheel (WTW) methodology is widely used for policy support in road transport. It can be seen as a simplified life cycle assessment (LCA) that focuses on the energy consumption and CO2 emissions only for the fuel being consumed, ignoring other stages of a vehicle’s life cycle. WTW results are therefore different from LCA results. In order to close this gap, the authors propose a hybrid WTW+LCA methodology useful to assess the greenhouse gas (GHG) profiles of road vehicles.

Methods

The proposed method (hybrid WTW+LCA) keeps the main hypotheses of the WTW methodology, but integrates them with LCA data restricted to the global warming potential (GWP) occurring during the manufacturing of the battery pack. WTW data are used for the GHG intensity of the EU electric mix, after a consistency check with the main life cycle impact (LCI) sources available in literature.

Results and discussion

A numerical example is provided, comparing GHG emissions due to the use of a battery electric vehicle (BEV) with emissions from an internal combustion engine vehicle. This comparison is done both according to the WTW approach (namely the JEC WTW version 4) and the proposed hybrid WTW+LCA method. The GHG savings due to the use of BEVs calculated with the WTW-4 range between 44 and 56 %, while according to the hybrid method the savings are lower (31–46 %). This difference is due to the GWP which arises as a result of the manufacturing of the battery pack for the electric vehicles.

Conclusions

The WTW methodology used in policy support to quantify energy content and GHG emissions of fuels and powertrains can produce results closer to the LCA methodology by adopting a hybrid WTW+LCA approach. While evaluating GHG savings due to the use of BEVs, it is important that this method considers the GWP due to the manufacturing of the battery pack.
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4.

Purpose

The cultivation of pomegranate worldwide has increased sharply in the past few years, mainly due to the growing perception that this fruit has numerous medical benefits. Despite the proliferation of studies delving into the properties of pomegranate from a medical and dietary perspective, its analysis from an environmental perspective has yet to be carried out in depth. Hence, the present study aims at understanding the life cycle environmental impacts in terms of greenhouse gas (GHG) emissions derived from the cultivation, processing and distribution abroad of fresh pomegranate grown at an innovative farm in a hyper-arid area in the region of Ica (Peru).

Methods

The international standards for life cycle methodologies were considered in order to obtain the overall carbon footprint (CFP) of fresh pomegranate cultivation, processing and distribution. Data acquisition was performed at the cultivation site and supported by the ecoinvent® database, whereas GHG emissions were modelled using the IPCC 2007 method. In addition, biogenic carbon sequestration was included in the assessment, using two distinct models, a first one to model the aerial carbon sequestered by the pomegranate trees and a second, using the IPCC Soil Carbon Tool for soil storage.

Results and discussion

Annual results show that on-site GHG emissions can be mitigated to a great extent in the first years of production thanks to biogenic carbon sequestration. However, through time, this tendency is reverted, and in years of maximum pomegranate productivity, GHG emissions are estimated to outweigh those linked to sequestration, despite the relevant minimization of emissions when using innovative irrigation schemes as compared to the conventional flood irrigation in the region.

Conclusions

Despite the threat in terms of water depletion and security, the expansion of Peru’s agricultural frontier in hyper-arid areas appears to be a feasible strategy for carbon fixation, although current agricultural practices, such as the use of machinery or electricity, need to be optimized to make positive the carbon balance.
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5.

Purpose

The purpose of this study was to quantify the spatial and technological variability in life cycle greenhouse gas (GHG) emissions, also called the carbon footprint, of durum wheat production in Iran.

Methods

The calculations were based on information gathered from 90 farms, each with an area ranging from 1 to 150 ha (average 16 ha). The carbon footprint of durum wheat was calculated by quantifying the biogenic GHG emissions of carbon loss from soil and biomass, as well as the GHG emissions from fertilizer application and machinery use, irrigation, transportation, and production of inputs (e.g., fertilizers, seeds, and pesticides). We used Spearman’s rank correlation to quantify the relative influence of technological variability (in crop yields, fossil GHG emissions, and N2O emissions from fertilizer application) and spatial variability (in biogenic GHG emissions) on the variation of the carbon footprint of durum wheat.

Results and discussion

The average carbon footprint of 1 kg of durum wheat produced was 1.6 kg CO2-equivalents with a minimum of 0.8 kg and a maximum of 3.0 kg CO2-equivalents. The correlation analysis showed that variation in crop yield and fertilizer application, representing technological variability, accounted for the majority of the variation in the carbon footprint, respectively 76 and 21%. Spatial variation in biogenic GHG emissions, mainly resulting from differences in natural soil carbon stocks, accounted for 3% of the variation in the carbon footprint. We also observed a non-linear relationship between the carbon footprint and the yield of durum wheat that featured a scaling factor of ?2/3. This indicates that the carbon footprint of durum wheat production (in kg CO2-eq kg?1) typically decreases by 67% with a 100% increase in yield (in kg ha?1 year?1).

Conclusions

Various sources of variability, including variation between locations and technologies, can influence the results of life cycle assessments. We demonstrated that technological variability exerts a relatively large influence on the carbon footprint of durum wheat produced in Iran with respect to spatial variability. To increase the durum wheat yield at farms with relatively large carbon footprints, technologies such as site-specific nutrient application, combined tillage, and mechanized irrigation techniques should be promoted.
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6.

Purpose

The aim of this study was to estimate the total greenhouse gas (GHG) emissions generated from whole life cycle stages of a sewer pipeline system and suggest the strategies to mitigate GHG emissions from the system.

Methods

The process-based life cycle assessment (LCA) with a city-scale inventory database of a sewer pipeline system was conducted. The GHG emissions (direct, indirect, and embodied) generated from a sewer pipeline system in Daejeon Metropolitan City (DMC), South Korea, were estimated for a case study. The potential improvement actions which can mitigate GHG emissions were evaluated through a scenario analysis based on a sensitivity analysis.

Results and discussion

The amount of GHG emissions varied with the size (150, 300, 450, 700, and 900 mm) and materials (polyvinyl chloride (PVC), polyethylene (PE), concrete, and cast iron) of the pipeline. Pipes with smaller diameter emitted less GHG, and the concrete pipe generated lower amount of GHG than pipes made from other materials. The case study demonstrated that the operation (OP) stage (3.67 × 104 t CO2eq year?1, 64.9%) is the most significant for total GHG emissions (5.65 × 104 t CO2eq year?1) because a huge amount of CH4 (3.51 × 104 t CO2eq year?1) can be generated at the stage due to biofilm reaction in the inner surface of pipeline. Mitigation of CH4 emissions by reducing hydraulic retention time (HRT), optimizing surface area-to-volume (A/V) ratio of pipes, and lowering biofilm reaction during the OP stage could be effective ways to reduce total GHG emissions from the sewer pipeline system. For the rehabilitation of sewer pipeline system in DMC, the use of small diameter pipe, combination of pipe materials, and periodic maintenance activities are suggested as suitable strategies that could mitigate GHG emissions.

Conclusions

This study demonstrated the usability and appropriateness of the process-based LCA providing effective GHG mitigation strategies at a city-scale sewer pipeline system. The results obtained from this study could be applied to the development of comprehensive models which can precisely estimate all GHG emissions generated from sewer pipeline and other urban environmental systems.
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7.

Purpose

Light-duty vehicles contribute considerably to global greenhouse gas emissions. Fuel cell vehicles (FCVs) may play a key role in mitigating these emissions without facing the same limitations in range and refueling time as battery electric vehicles (BEVs). In this study, we assess the environmental impacts and costs of a polymer electrolyte membrane fuel cell system (FCS) for use in light-duty FCVs and integrate these results into a comparative evaluation between FCVs, BEVs, and internal combustion engine vehicles (ICEVs).

Methods

We conduct a detailed life cycle assessment (LCA) and cost assessment for the current state of the technology and two future scenarios for technological development. We compile a detailed and consistent inventory for the FCS by systematically disassembling and integrating information found in cost studies. For the vehicle-level comparison, we use models to ensure that vehicle size, performance, and fuel consumption are unbiased between vehicle types and consistent with the scenarios for technological development.

Results and discussion

Our results show that FCVs can decrease life cycle greenhouse gas emissions by 50 % compared to gasoline ICEVs if hydrogen is produced from renewable electricity, thus exhibiting similar emission levels as BEVs that are charged with the same electricity mix. If hydrogen is produced by natural gas reforming, FCVs are found to offer no greenhouse gas reductions, along with higher impacts in several other environmental impact categories. A major contributor to these impacts is the FCS, in particular the platinum in the catalyst and the carbon fiber in the hydrogen tank. The large amount of carbon fiber used in the tank was also the reason why we found that FCVs may not become fully cost competitive with ICEVs or BEVs, even when substantial technological development and mass production of all components is assumed.

Conclusions

We conclude that FCVs only lead to lower greenhouse gas emissions than ICEVs if their fuel is sourced from renewable energy, as is the case with BEVs. FCVs are an attractive alternative to ICEVs in terms of vehicle performance criteria such as range and refueling time. However, the technological challenges associated with reducing other environmental impacts and costs of FCVs seem to be as large, if not larger, than those associated with the capacity and costs of batteries for BEVs—even when not taking into account the efforts required to build a hydrogen infrastructure network for road transportation.
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8.

Purpose

The fifth assessment report by the IPCC includes methane oxidation as an additional indirect effect in the global warming potential (GWP) and global temperature potential (GTP) values for methane. An analysis of the figures provided by the IPCC reveals they lead to different outcomes measured in CO2-eq., depending on whether or not biogenic CO2 emissions are considered neutral. In this article, we discuss this inconsistency and propose a correction.

Methods

We propose a simple framework to account for methane oxidation in GWP and GTP in a way that is independent on the accounting rules for biogenic carbon. An equation with three components is provided to calculate metric values, and its application is tested, together with the original IPCC figures, in a hypothetical example focusing on GWP100.

Results and discussion

The hypothetical example shows that the only set of GWP100 values consistently leading to the same outcome, regardless of how we account for biogenic carbon, is the one proposed in this article. Using the methane GWP100 values from the IPCC report results in conflicting net GHG emissions, thus pointing to an inconsistency.

Conclusions

In order to consistently discriminate between biogenic and fossil methane sources, a difference of 2.75 kg CO2-eq. is needed, which corresponds to the ratio of the molecular weights of CO2 and methane (44/16). We propose to correct the GWP and GTP values for methane accordingly.
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9.

Purpose

Governments around the world encourage the use of biofuels through fuel standard policies that require the addition of renewable diesel in diesel fuel from fossil fuels. Environmental impact studies of the conversion of biomass to renewable diesel have been conducted, and life cycle assessments (LCA) of the conversion of lignocellulosic biomass to hydrogenation-derived renewable diesel (HDRD) are limited, especially for countries with cold climates like Canada.

Methods

In this study, an LCA was conducted on converting lignocellulosic biomass to HDRD by estimating the well-to-wheel greenhouse gas (GHG) emissions and fossil fuel energy input of the production of biomass and its conversion to HDRD. The approach to conduct this LCA includes defining the goal and scope, compiling a life cycle inventory, conducting a life cycle impact assessment, and executing a life cycle interpretation. All GHG emissions and fossil fuel energy inputs were based on a fast pyrolysis plant capacity of 2000 dry tonnes biomass/day. A functional unit of 1 MJ of HDRD produced was adopted as a common unit for data inputs of the life cycle inventory. To interpret the results, a sensitivity analysis was performed to measure the impact of variables involved, and an uncertainty analysis was performed to assess the confidence of the results.

Results and discussion

The GHG emissions of three feedstocks studied—whole tree (i.e., chips from cutting the whole tree), forest residues (i.e., chips from branches and tops generated from logging operations), and agricultural residues (i.e., straw from wheat and barley)—range from 35.4 to 42.3 g CO2,eq/MJ of HDRD (i.e., lowest for agricultural residue- and highest for forest residue-based HDRD); this is 53.4–61.1 % lower than fossil-based diesel. The net energy ratios range from 1.55 to 1.90 MJ/MJ (i.e., lowest for forest residue- and highest for agricultural residue-based HDRD) for HDRD production. The difference in results among feedstocks is due to differing energy requirements to harvest and pretreat biomass. The energy-intensive hydroprocessing stage is responsible for most of the GHG emissions produced for the entire conversion pathway.

Conclusions

Comparing feedstocks showed the significance of the efficiency in the equipment used and the physical properties of biomass in the production of HDRD. The overall results show the importance of efficiency at the hydroprocessing stage. These findings indicate significant GHG mitigation benefits for the oil refining industry using available lignocellulosic biomass to produce HDRD for transportation fuel.
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10.

Background

Lung ischemia-reperfusion (IR) injury after transplantation as well as acute shortage of suitable donor lungs are two critical issues impacting lung transplant patients. This study investigates the anti-inflammatory and immunomodulatory role of human mesenchymal stromal cells (MSCs) and MSC-derived extracellular vesicles (EVs) to attenuate lung IR injury and improve of ex-vivo lung perfusion (EVLP)-mediated rehabilitation in donation after circulatory death (DCD) lungs.

Methods

C57BL/6 wild-type (WT) mice underwent sham surgery or lung IR using an in vivo hilar-ligation model with or without MSCs or EVs. In vitro studies used primary iNKT cells and macrophages (MH-S cells) were exposed to hypoxia/reoxygenation with/without co-cultures with MSCs or EVs. Also, separate groups of WT mice underwent euthanasia and 1 h of warm ischemia and stored at 4 °C for 1 h followed by 1 h of normothermic EVLP using Steen solution or Steen solution containing MSCs or EVs.

Results

Lungs from MSCs or EV-treated mice had significant attenuation of lung dysfunction and injury (decreased edema, neutrophil infiltration and myeloperoxidase levels) compared to IR alone. A significant decrease in proinflammatory cytokines (IL-17, TNF-α, CXCL1 and HMGB1) and upregulation of keratinocyte growth factor, prostaglandin E2 and IL-10 occurred in the BAL fluid from MSC or EV-treated mice after IR compared to IR alone. Furthermore, MSCs or EVs significantly downregulated iNKT cell-produced IL-17 and macrophage-produced HMGB1 and TNF-α after hypoxia/reoxygenation. Finally, EVLP of DCD lungs with Steen solution including MSCs or EVs provided significantly enhanced protection versus Steen solution alone. Co-cultures of MSCs or EVs with lung endothelial cells prevents neutrophil transendothelial migration after exposure to hypoxia/reoxygenation and TNF-α/HMGB1 cytomix.

Conclusions

These results suggest that MSC-derived EVs can attenuate lung inflammation and injury after IR as well as enhance EVLP-mediated reconditioning of donor lungs. The therapeutic benefits of EVs are in part mediated through anti-inflammatory promoting mechanisms via attenuation of immune cell activation as well as prevention of endothelial barrier integrity to prevent lung edema. Therefore, MSC-derived EVs offer a potential therapeutic strategy to treat post-transplant IR injury as well as rehabilitation of DCD lungs.
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11.

Purpose

The emission of greenhouse gases (GHG) is a key criterion in the environmental assessment of biofuels. Life cycle inventories taking into account the latest methodological developments are an essential prerequisite for this assessment. In the last years, substantial progresses in the modelling of nitrogen emissions relevant for the climate as well as in modelling the emissions from land use change (LUC) have been achieved. Therefore, the biomass production inventories in the ecoinvent database were revised to take into account these developments.

Methods

The IPCC method tier 1 has been used for the assessment of N2O emissions. Induced emissions from NH3 and NO3 were included as well. Due to the importance of the latter emissions for N2O formation, these emissions have also been updated and harmonised. The Agrammon model was used for the NH3 emissions. The SALCA-NO3 model has been applied in the European inventories to estimate nitrate leaching, whilst in non-European inventories the SQCB-NO3 model has been used. The quantification of the land use change areas has been based on annualized, retrospective data of the last 20 years. All carbon pools (from aboveground biomass to soil organic carbon) were considered and differentiated on a regional level for all of the natural vegetation categories affected. Whenever possible, default values and methods from the IPCC 2006 were applied.

Results and discussion

The changes for ammonia emissions were generally very small (?5 % on average). The nitrate emissions increased on average by +13 %, but this slight trend is the result of important downward and upward changes, whilst the average N2O emissions decreased by ?26 %. For the existing inventories of soybean, palm oil and sugarcane production, significant increases of GHG emissions resulted from LUC modelling. This was mainly due to the consistent inclusion of all carbon stocks according to the IPCC guidelines. The calculation method can also result in important C sequestration effects in certain cases like African Jatropha production.

Conclusions

The changes in greenhouse gas emissions due to the updated methodology were significant. This shows that life cycle assessment studies for biofuels using older methodological bases need to be revised and could lead to different conclusions. The implemented and cultivated superstructure for LUC modelling is modular and flexible and can be easily extended to other important crop activities. The new parameterisation functionality applied for the activities provides powerful means for the simple generation of site-specific activities.
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12.

Purpose

The crude palm oil (CPO) extraction is normally done by a wet extraction process, and wastewater treatment of the wet process emits high levels of greenhouse gases (GHGs). A dry process extracts mixed palm oil (MPO) from palm fruit without using water and has no GHG emissions from wastewater treatment. This work is aimed at determining the GHG emissions of a dry process and at evaluating GHG savings on changing from wet to dry process, including land use change (LUC) effects.

Methods

Life cycle assessment from cradle to gate was used. The raw material is palm fruits. The dry process includes primary production, oil room, and utilities. MPO is the main product, while palm cake and fine palm residue are co-products sold for animal feed. Case studies were undertaken without and with carbon stocks of firewood and of nitrogen recycling at plantations from fronds. Allocations by mass, economic, and heating values were conducted. The trading of GHG emissions from co-products to GHG emissions from animal feed was assessed. The GHG emissions or savings from direct LUC (dLUC) and from indirect LUC (iLUC) effects and for the change from wet to dry process were determined.

Results and discussion

Palm fruit and firewood were the major GHG emission sources. Nitrogen recycling on plantations from fronds significantly affects the GHG emissions. With the carbon stocks, the GHG emissions allocated by energy value were 550 kg CO2 eq/t MPO. The GHG emissions were affected by ?3 to 37% for the change from wet to dry process. When the plantation area was increased by 1 ha and the palm oil extraction was changed from wet to dry process, and the change included dLUC and iLUC, the GHG savings ranged from ?0.94 to 5.08 t CO2 eq/ha year. The iLUC was the main GHG emission source. The GHG saving mostly originated from the change of extraction process and from the dLUC effect. Based on the potential use of biodiesel production from oil palm, during 2015–2036 in Thailand, when the extraction process was changed and dLUC and iLUC effects were included, the saving in GHG emissions was estimated to range from ?35,454 to 274,774 t CO2 eq/year.

Conclusions

The change of palm oil extraction process and the LUC effects could minimize the GHG emissions from the palm oil industry. This advantage encourages developing policies that support the dry extraction process and contribute to sustainable developments in palm oil production.
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13.

Purpose

Mangrove forests have been recognized as important regulators of greenhouse gases (GHGs), yet the resulting land use and land-use change (LULUC) emissions have rarely been accounted for in life cycle assessment (LCA) studies. The present study therefore presents up-to-date estimates for GHG emissions from mangrove LULUC and applies them to a case study of shrimp farming in Vietnam.

Methods

To estimate the global warming impacts of mangrove LULUC, a combination of the International Panel for Climate Change (IPCC) guidelines, the Net Committed Emissions, and the Missed Potential Carbon Sink method were used. A literature review was then conducted to characterize the most critical parameters for calculating carbon losses, missed sequestration, methane fluxes, and dinitrogen monoxide emissions.

Results and discussion

Our estimated LUC emissions from mangrove deforestation resulted in 124 t CO2 ha?1 year?1, assuming IPCC’s recommendations of 1 m of soil loss, and 96% carbon oxidation. In addition to this, 1.25 t of carbon would no longer be sequestered annually. Discounted over 20 years, this resulted in total LULUC emissions of 129 t CO2 ha?1 year?1 (CV = 0.441, lognormal distribution (ln)). Shrimp farms in the Mekong Delta, however, can today operate for 50 years or more, but are 1.5 m deep (50% oxidation). In addition to this, Asian tiger shrimp farming in mixed mangrove concurrent farms (the only type of shrimp farm that resulted in mangrove deforestation since 2000 in our case study) resulted in 533 kg methane and 1.67 kg dinitrogen monoxide per hectare annually. Consequently, the LULUC GHG emissions resulted in 184 and 282 t CO2-eq t?1 live shrimp at farm gate, using mass and economic allocation, respectively. These GHG emissions are about an order of magnitude higher than from semi-intensive or intensive shrimp farming systems. Limitations in data quality and quantity also led us to quantify the uncertainties around our emission estimates, resulting in a CV of between 0.4 and 0.5.

Conclusions

Our results reinforce the urgency of conserving mangrove forests and the need to quantify uncertainties around LULUC emissions. It also questions mixed mangrove concurrent shrimp farming, where partial removal of mangrove forests is endorsed based upon the benefits of partial mangrove conservation and maintenance of certain ecosystem services. While we recognize that these activities limit the chances of complete removal, our estimates show that large GHG emissions from mangrove LULUC question the sustainability of this type of shrimp farming, especially since mixed mangrove farming only provide 5% of all farmed shrimp produced in Vietnam.
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14.

Introduction

Data sharing is being increasingly required by journals and has been heralded as a solution to the ‘replication crisis’.

Objectives

(i) Review data sharing policies of journals publishing the most metabolomics papers associated with open data and (ii) compare these journals’ policies to those that publish the most metabolomics papers.

Methods

A PubMed search was used to identify metabolomics papers. Metabolomics data repositories were manually searched for linked publications.

Results

Journals that support data sharing are not necessarily those with the most papers associated to open metabolomics data.

Conclusion

Further efforts are required to improve data sharing in metabolomics.
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15.

Purpose

Eco-labelling has become part of the business strategy of companies thanks to numerous advantages in terms of engaging with consumers and gaining market quota. The aim of this article is to present a critical discussion on the development and implementation of a new eco-label named pescaenverde, registered in Spain, as the first type III eco-label in the Spanish fishing sector that is based on life cycle approaches for seafood products.

Methods

More specifically, it aims to complement ecosystem-based eco-labels with the computation of the carbon footprint and the energy return on investment (EROI) of seafood products. Furthermore, it proposes to discuss the ecological criteria, certification process or the opportunities and challenges of the market implementation of this eco-label in detail. Finally, the authors argue that life cycle eco-labels should be considered important complements for more specific sector- or ecosystem-oriented labels already in use, rather than direct competitors.

Results and discussion

There has been much criticism towards the eco-labelling sector as regards the transparency and scientific rigour of its standards. The fishing and seafood sector, which has experienced a boom in eco-labelling in recent years, due mainly to the strength of the Marine Stewardship Council certification scheme, is not alien to this controversy, since critics advocate expanding the concept of sustainable fisheries beyond an ecosystem approach in order to account for global environmental concerns such as greenhouse gas (GHG) emissions or energy use. Not surprisingly, the European Union and other authorities currently encourage eco-labels to base their ecological criteria on life cycle approaches. Therefore, the current study discusses the ecological criteria, certification process or the opportunities and challenges of the market implementation of this eco-label in detail.

Conclusions

The specificity of the life cycle inventory scheme used in pescaenverde delivers an accurate computation of environmental impacts for the specific case of Spanish fisheries. However, the geographical expansion of this scheme to other nations or regions will be conditioned by an important software adaptation to the particular inventory characteristics of the new fisheries, fleets and products.

Recommendations

Adapting ecological criteria to other situations would also need substantial discussion, since the use of this certification scheme is not intended to contrast or compare seafood products against each other but to provide consumers with an easily identifiable label through which they can detect environmentally sustainable practices in terms of GHG emissions and energy use in the fishing fleets supporting the seafood products purchased.
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16.

Purpose

As one of largest energy consumers, the transport sector (TS) has significant impacts on the environment. Shenzhen, a developed megacity in South China, plays a leadership role in promoting the development of energy efficient vehicles in China.

Methods

This paper aims to assess the carbon footprint (CF) of the TS in Shenzhen via a Streamlined Life Cycle Assessment method. Consequently, the current environmental performance of the TS is evaluated and improvement potentials are examined.

Results and discussion

The results show that CF has gained rapid growth over the past decade at an annual rate of 15.3 %, closely corresponding with the growth of the Gross Domestic Product (18.9 %) in Shenzhen. The total CF in 2013 was estimated as 50.7 million tons (ranging from 41.7 to 59.9). Road based freight transport accounts for the largest share of the TS’ emissions. The most significant contributors in this sector are: light duty trucks, urban public transport bus service, and passenger air transport. Meanwhile, this study took new energy vehicles into consideration in order to explore the range of CF mitigation potential in Shenzhen. The potential carbon abatement is not significant in comparison with the impact growth derived from the increasing freight and passenger transport based on the assumption that the transport intensity and its annual growth rate maintain at the current levels.

Conclusions

This study offers a useful approach to evaluate the available options for sustainable transport system planning in Shenzhen. For carbon emissions reductions from the TS, policies and technological innovations are essential to facilitate the transition to a low carbon TS. In addition, the methodology developed in this study could be used for assessing CF in other sectors.
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17.

Introduction

Untargeted metabolomics is a powerful tool for biological discoveries. To analyze the complex raw data, significant advances in computational approaches have been made, yet it is not clear how exhaustive and reliable the data analysis results are.

Objectives

Assessment of the quality of raw data processing in untargeted metabolomics.

Methods

Five published untargeted metabolomics studies, were reanalyzed.

Results

Omissions of at least 50 relevant compounds from the original results as well as examples of representative mistakes were reported for each study.

Conclusion

Incomplete raw data processing shows unexplored potential of current and legacy data.
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18.

Introduction

Data processing is one of the biggest problems in metabolomics, given the high number of samples analyzed and the need of multiple software packages for each step of the processing workflow.

Objectives

Merge in the same platform the steps required for metabolomics data processing.

Methods

KniMet is a workflow for the processing of mass spectrometry-metabolomics data based on the KNIME Analytics platform.

Results

The approach includes key steps to follow in metabolomics data processing: feature filtering, missing value imputation, normalization, batch correction and annotation.

Conclusion

KniMet provides the user with a local, modular and customizable workflow for the processing of both GC–MS and LC–MS open profiling data.
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19.

Introduction

It is difficult to elucidate the metabolic and regulatory factors causing lipidome perturbations.

Objectives

This work simplifies this process.

Methods

A method has been developed to query an online holistic lipid metabolic network (of 7923 metabolites) to extract the pathways that connect the input list of lipids.

Results

The output enables pathway visualisation and the querying of other databases to identify potential regulators. When used to a study a plasma lipidome dataset of polycystic ovary syndrome, 14 enzymes were identified, of which 3 are linked to ELAVL1—an mRNA stabiliser.

Conclusion

This method provides a simplified approach to identifying potential regulators causing lipid-profile perturbations.
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20.

Purpose

Palm biodiesel life cycle studies have been mainly performed for Asia and focused on greenhouse gas (GHG) intensity. The purpose of this article is to present an environmental life cycle assessment (LCA) of biodiesel produced in Portugal from palm oil (PO) imported from Colombia, addressing the direct effects of land-use change (LUC), different fertilization schemes, and biogas management options at the extraction mill.

Methods

An LC inventory and model of PO biodiesel was implemented based on data collected in five Portuguese biodiesel plants and in a palm plantation and extraction mill in the Orinoquía Region of Colombia. The emissions due to carbon stock changes associated with LUC were calculated based on the Colombian oil palm area expansion from 1990 to 2010 and on historical data of vegetation cleared for planting new palm trees. Five impact categories were assessed based on ReCiPe and CML-IA methods: GHG intensity, freshwater and marine eutrophication, photochemical oxidant formation, terrestrial acidification. A sensitivity analysis of alternative allocation approaches was performed.

Results and discussion

Palm plantation was the LC phase which contributed the most to eutrophication and acidification impacts, whereas transportation and oil extraction contributed the most to photochemical oxidation. An increase in carbon stock due to LUC associated with the expansion of Colombian oil palm was calculated (palm is a perennial crop with higher carbon stock than most previous land-uses). The choice of the fertilization scheme that leads to the lowest environmental impacts is contradictory among various categories. The use of calcium ammonium nitrate (followed by ammonium sulfate) leads to the lowest acidification and eutrophication impacts. The highest GHG intensity was calculated for calcium ammonium nitrate, while the lowest was for ammonium sulfate and poultry manure. Biogas captured and flared at the oil extraction mill instead of being released into the atmosphere had the lowest impacts in all categories (GHG intensity reduced by more than 60 % when biogas is flared instead of released).

Conclusions

Recommendation on the selection of the fertilization scheme depends on the environmental priority. ReCiPe and CML showed contradictory results for eutrophication and photochemical oxidation; however, uncertainty may impair strong recommendations. GHG intensity and photochemical oxidation impacts can be significantly reduced if biogas is flared instead of being released. However, more efficient biogas management should be implemented in order to reduce the impacts further.
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