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1.
Mingjia Yang Xiangming Xie Xiaoqing He Fangqiu Zhang 《Plant Cell, Tissue and Organ Culture》2006,85(2):241-245
In this paper we report the establishment of Acacia crassicarpa regeneration through organogenesis. We used phyllode (leaf) explants excised from 60-day-old in vitro seedlings for green compact nodule induction and, tested Murashige and Skoog (MS) media supplemented with various concentrations of 1-phenyl-3-(thiadiazol-5-yl) urea (thidiazuron) (TDZ) and α-naphthaleneacetic acid (NAA). Under the optimized condition, green compact nodules and adventitious shoots were induced in 10 and 40 days, respectively, on the medium containing a combination of 0.5 mg l−1 TDZ and 0.5 mg l−1 NAA. This medium also yielded the highest rate (56%) of adventitious shoots forming from the nodules. Efficient shoot elongation was achieved by transferring the clusters of adventitious shoots to medium containing 0.1 mg l−1 TDZ within 2 months. The elongated adventitious shoots were rooted at a rate of 96.5% on half-strength MS medium with 0.5 mg l−1 3-indolebutyric acid (IBA) in 1 month. Rooted plantlets were hardened and successfully established in soil with an 80% survival rate. To our knowledge, this is the first report describing a detailed protocol for regeneration through organogenesis using phyllodes as explants for A. crassicarpa. 相似文献
2.
M. Hossain B. K. Biswas M. R. Karim S. Rahman R. Islam O. I. Joarder 《Plant Cell, Tissue and Organ Culture》1994,39(3):265-268
Elephant apple (Feronia limonia L.). was micropropagated on MS medium containing 4.4 M benzyladenine and 4.6 M kinetin using cotyledon explants taken from in vitro-grown seedlings. Adventitious buds formed on the cotyledon developed into shoots that were rooted in half-strength MS medium containing 0.57 M indoleacetic acid and 0.49 M indolebutyric acid. Plants were successfully established in soil.Abbreviations BA
6-benzyladenine
- IAA
3-indoleacetic acid
- IBA
3-indolebutyric acid
- MS
Murashige & Skoog 相似文献
3.
In vitro propagation was initiated from 2-week-old and 7-month-old explants of Acacia mangium. Juvenile explants (2 week-old) of 5- to 10-mm lengths composed of two leaves were cultured on Murashige and Skoog (MS) medium
containing 1.0 or 2.0 mg L-1 6-benzyladenine (BAP). After 6 weeks, most explants had formed a large cluster of 14–18 axillary shoots produced by prolific
branching of the primary axillary shoot after elongation. The maximum multiplication rate (40) was obtained in the first subculture;
the rate decreased to 10–20 in the second one. The mean length of shoots was not significantly affected by BAP concentrations
during the subsequent cultures. Rooting ability of juvenile explants was greatly affected by BAP concentrations used in the
multiplication medium. When both types of explants were multiplied on a MS medium containing 1.0 mg L-1 BAP and transferred to a half-strength MS medium containing 0.05 mg L-1 IBA, only 10% of the juvenile explants were rooted versus 70% of the 7-month-old explants. Rooted plants transferred onto
artificial substrate were all nodulated, when inoculated with a specific Bradyrhizobium sp. strain. 相似文献
4.
Vengadesan G. Ganapathi A. Prem Anand R. Ramesh Anbazhagan V. 《Plant Cell, Tissue and Organ Culture》2000,61(1):23-28
In vitro morphogenesis via organogenesis was achieved from callus cultures derived from hypocotyl explants of Acacia sinuata on MS (Murashige and Skoog, 1962) medium. Calli were induced from hypocotyl explants excised from 7-day-old seedlings on
MS medium containing 3% sucrose, 0.8% agar, 6.78 μM 2,4-dichlorophenoxyacetic acid and 2.22 μM 6-benzylaminopurine. Regeneration
of adventitious buds from callus was achieved when they were cultured on MS medium supplemented with 10% coconut water, 13.2
μM 6-benzylaminopurine and 3.42 μM indoleacetic acid. Addition of gibberellic acid (1.73 μM) favored shoot elongation. Regenerated
shoots produced prominent roots when transferred to half strength MS medium supplemented with 7.36 μM indolebutyric acid.
Rooted plantlets, thus developed were hardened and successfully established in the soil. This protocol yielded an average
of 20 plants per hypocotyl explant over a period of 4 months.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Manjula S. Thomas Anita Daniel Benny Nair G.M. 《Plant Cell, Tissue and Organ Culture》1997,51(2):145-148
Protocols for in vitro plant regeneration via axillary and adventitious shoot regeneration were established in an important
medicinal plant, Aristolochia indica L. (Aristolochiaceae). Basal Murashige and Skoog's (MS) medium supplemented with 0.54
μM α-naphthaleneacetic acid (NAA) and 13.31 μM benzyladenine (BA) induced the maximum number of shoots (45-50) from shoot
tip and nodal segment cultures. Phenolic accumulation in leaf and internodal stem derived callus cultured in MS medium containing
NAA or 2,4-dichlorophenoxyacetic acid and BA or kinetin was controlled by the addition of 1.0 mg l-1 phloroglucinol (PG) to the callus induction medium. Basal medium supplemented with 2.69 μM NAA, 13.31 μM BA and 1.0 mg l-1 PG induced the best results in terms of shoot bud regeneration from leaf derived callus. Direct de novo development of shoots
from leaf segments was achieved using 13.31 μM BA along with 50 mg l-1 activated charcoal. The microshoots were rooted in White's medium supplemented with 2.46 μM indolebutyric acid. More than
85% of rooted plants survived in the soil.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
Kristen L. Choffe Jerrin M. R. Victor Susan J. Murch Praveen K. Saxena 《In vitro cellular & developmental biology. Plant》2000,36(1):30-36
Summary An in vitro propagation system was developed for Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Echinacea seeds were found to be contaminated with systemic fungi and therefore an optimized minimal concentration of Plant Preservation
Mixture (PPM) was incorporated in the seed germination medium to recover sterile seedlings. Regeneration was induced on petiole
explants from 2-month-old sterile seedlings cultured on medium supplemented with benzylaminopurine (BAP) or thidiazuron (TDZ)
in combination with indoleacetic acid (IAA). Two distinct forms of regeneration were identified in cultured petiole explants
with histological and morphological observations, viz. the direct formation of somatic embryos on the epidermis and the de novo development of shoots from callus tissues formed in subepidermal cell layers. the results of this study have established
a micropropagation system for E. purpurea that will provide sterile plant material for further investigations into medicinally active biochemicals and may facilitate
mass production of high-quality E. purpurea plants for the commercial market. 相似文献
7.
B. N. S. Murthy Jerrin Victor Rana P. Singh R. A. Fletcher Praveen K. Saxena 《Plant Growth Regulation》1996,19(3):233-240
In vitro regeneration in chickpea (Cicer arietinum L.) was achieved by direct culture of mature seeds on Murashige and Skoog (MS) medium supplemented with either N-phenyl-N(-1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ) or N6-benzylaminopurine (BAP). Multiple shoots formed de novo without an intermediary callus phase at the cotyledonary notch region of the seedlings within 2 to 3 weeks of culture initiation. TDZ was found to be more effective compared to BAP as an inductive signal of regeneration. The former induced multiple shoot formation at all the concentrations tested (1 M to 100 M), although, maximum morphogenic response was observed at 10 M concentration. Addition of naphthaleneacetic acid (NAA) alone or in combination with BAP to the MS medium failed to invoke a similar response. When the TDZ supplemented medium was amended with L-proline, the resultant regenerants were mostly somatic embryos. Histological investigations confirmed the switch in the regeneration pathway from directly formed adventitious shoots to embryogenesis. For obtaining plantlets, adventitious shoots were rooted on MS medium supplemented with 2.5 M NAA; somatic embryos were germinated and established on MS medium. Normal plants were regenerated from both adventitious shoots and somatic embryos and transferred to soil.Abbreviations BAP
6-benzylaminopurine
- MS
Murashige and Skoog [14] basal medium
- NAA
naphthaleneacetic acid
- TDZ
thidiazuron [N-phenyl-N(-1,2,3,-thidiazol-5-yl)-urea] 相似文献
8.
Q. L. Xu Y. H. Xie H. Ru X. Hu Ch. Y. Wang X. Yu. Wang 《Russian Journal of Plant Physiology》2009,56(4):546-550
An efficient system for in vitro regeneration of red leaf beet, a variety of leaf beet (Beta vulgaris L. var cicla L.) generally used to decorate parterre and to prepare betacyanin, was developed for the first time in the present study.
Shoot tip and petiole explants from the sterile seedlings, precultured on Murashige and Skoog (MS) medium with 15 mg/l 6-benzyladenine
(BA) and 3% sucrose at 16 °C for 30 days, could form 81.02 and 17.33% translucent nodular (TN) calli, respectively. All TN
calli were able to differentiate into adventitious shoots under the same culture conditions. Each explant with TN callus from
the shoot tip and petiole could generate 8.65 shoots on average. It was found that both preculture of sterile seedlings and
culture of explants at low temperature (16 °C) were vital for TN callus induction and adventitious bud formation of red leaf
beet. The best condition for rooting was 0.5-strength MS medium with 10 g/l sucrose. After being transplanted into soil, plantlets
grew well and could flower and bear fruits. Histological observation revealed that TN callus was derived from the cells of
vascular tissue of the petiole and that adventitious shoots were formed through organogenesis. The factors influencing in
vitro micropropagation are also discussed.
Published in Russian in Fiziologiya Rastenii, 2009, Vol. 56, No. 4, pp. 603–608.
This text was submitted by the autors in English. 相似文献
9.
Gerard C. Douglas John Mcnamara 《In vitro cellular & developmental biology. Plant》2000,36(5):412-415
Summary Regeneration of adventitious shoots was obtained in over 80% of explants, consisting of wounded cotyledonary nodes of Acacia mangium, by culturing germinated seedlings on DKW medium with combinations of N6-benzyladenine and either thidiazuron or N-(2-chloro-4-pyridyl)-N-phenylurea. Electron microscopy showed the presence of adventitious buds arising from wound tissue of the cotyledons and
cotyledonary nodes. Shoot regeneration was also obtained at lower frequency in isolated cotyledon explants cultured with 6%
sucrose alone (10%), or with 3% sucrose and 30.0 mg l−1 (0.1 μM) 2–4-dichlorophenoxyacetic acid (2,4-D; 16%). With 2,4-D,>60% of explants produced organized structures but these did not
develop into shoots or somatic embryos. Shoot formation was not induced in either hypocotyl or root explants. 相似文献
10.
Monacelli Barbara Pasqua Gabriella Cuteri Angelina Vitali Alberto 《Plant Cell, Tissue and Organ Culture》1999,58(2):81-85
A protocol for in vitro plant regeneration through organogenesis was established for Vismia guianensis(Hypericaceae), a species that produces an anti-cancer compound. The highest mean number of shoots per gram of callus (57.33)
was obtained on Murashige and Skoog medium supplemented with 4.44 μM 6-benzyl-aminopurine, 5.70 μM indole-3-acetic acid and
12.88 μM gibberellic acid. Rooting was favoured by the addition of 10 μM indole-3-butyric acid, and by sucrose concentrations
higher than 1%.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
11.
Plant regeneration via organogenesis in marigold 总被引:6,自引:0,他引:6
Vanegas Pablo E. Cruz–Hernández Andrés Valverde Ma. Elena Paredes–López Octavio 《Plant Cell, Tissue and Organ Culture》2002,69(3):279-283
Regeneration of whole plants of marigold (Tagetes erecta L.) was achieved by organogenesis using leaf explants. Leaf segments about 0.25 cm2 were taken from 3-week-old in vitro plantlets and cultured on MS basal medium containing BA with different auxins (NAA, 2,4-D and IAA). The exposure time of the explants on the regeneration medium was tested. The highest values for regeneration were obtained with BA (13.3 M) and IAA (17.1 M). Thirteen days was the best time of exposure of the explant to the regeneration medium for shoot induction. 相似文献
12.
Culturing pedicle segments of primroses on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2, 4-D) and thidiazuron (TDZ) resulted in callus induction rates of about 80%. The highest shoot regeneration rate (1.8 shoots per explant; mean of ten genotypes) was achieved with the combination of 2.0 mg/l 2, 4-D and 2.0 mg/l TDZ. Culture on a medium containing a high concentration of nitrate (for example, B5 medium) negatively affected the survival of regenerated shoots of one genotype, Gelb IV 48, probably due to an increase in the pH value of the medium. Consequently, the highest efficiency was obtained using a basal medium containing half-strength Murashige and Skoog macroelements. A protocol to regenerate shoots of Primula vulgaris and P. elatior is described. 相似文献
13.
A. Pellegrineschi 《Plant cell reports》1997,17(2):89-95
Shoot regeneration via organogenesis was achieved from axenic cowpea [Vigna unguiculata subsp. unguiculata L. (Walp.) Verde.] hypocotyls and cotyledons of advanced breeding lines and varieties. Cotyledons and embryos were excised
from green immature pods. The apical parts of the embryos were removed and the hypocotyls were transferred to regeneration
media. Cotyledons and hypocotyls were tested on media with gradients of several hormonal and putrescine combinations. Cowpea
cotyledons and hypocotyls exhibited a pattern of shoot formation that occurred in three distinct phases. Multiple shoots developed
within 45 days from the wounded region of the primary hypocotyl and cotyledons in different media containing a high cytokinin
concentration. The induced plant explants were then grown for 20 days in low-intensity light (10 μmol m–2 s–1) on the same medium and numerous shoot buds emerged de novo from the upper part of the hypocotyl and the wounded part of the cotyledons. These buds had no apparent vascular connection
with the parent tissues. The plant regeneration capability of this procedure was tested with several cowpea genotypes, five
of which (83D-442, 86D-1010, 93K-624, Vita 3 and Ife Brown) responded positively with shoot development and were able to form
roots and whole plants. Some somaclonal variation was observed.
Received: 14 June 1996 / Revision received: 14 December 1996 / Accepted: 25 January 1997 相似文献
14.
Different vegetative parts of Brassica alboglabra seedlings and mature plants were used as explants in culture.A high frequency (60–100%) of shoot regeneration was obtained from hypocotyl explants, nodal stem segments, internodal segments and shoot apices cultured on Murashige-Skoog basal medium. Addition of 6-benzylaminopurine and kinetin increased the average number of shoots per explant. When detached and transferred to basal medium, the shoots readily developed roots. Regenerated plantlets could be successfully transplanted in soil. 相似文献
15.
Callus was initiated from in vitro grown immature leaf and ex vitro grown mature leaf and rhizome explants of Agave sisalana Perr. ex. Engelm, on MS medium containing 2,4-D (9.05 M) and kinetin (4.6 M) or 2,4-D (9.05 M), kinetin (4.6 M) and CH (1000 mg l–1) or mod. MS (NH4NO3, 1500 mg l–1) containing 2,4-D (9.05 M) and kinetin (4.6 M). Light was essential for callus formation which, however, was different in three types of explants on three different media compositions. Increasing NH4
+had a negative impact while addition of CH had a positive impact on callus formation. Shoot regeneration from callus from CH-supplemented medium only was achieved for rhizome and immature leaf tissues. The highest rate of regeneration was obtained with BA (26.6 M) as the sole hormone. Shoot buds g–1 callus varied according to BA concentrations. Shoot proliferation rate increased on half-strength MS medium containing BA (8.9 M). Microshoots developed on MS medium containing BA (2.22 M) and GA3 (1.44 M) and finally rooted on MS medium containing IAA (11.42 M). Acclimatized rooted plantlets are growing satisfactorily in ex vitro. This is the first report on plant regeneration via organogenesis of A. sisalana. 相似文献
16.
This study reports a protocol for in vitro regeneration of Salvia x jamensis J. Compton starting from nodal explants of axenic plants and using Murashige and Skoog basal medium containing 0, 3.0, 6.0 or 12.0 μM thidiazuron. The addition of 3.0 μM 6-benzyladenine increases the production of regenerated shoots. 相似文献
17.
Root segments (1 cm long) were excised from 15–20 day old seedlings of silktree (Albizzia julibrissin) grown on B5 medium. About 50% of the control (no growth regulators added) root explants formed shoot buds within 15 days after placement on the culture medium. After 30 days, there were about 4 shoots per control explant. Addition of low levels of various auxins (0.5 M) did not influence the formation of shoot buds from the explants. Higher concentrations (5M), however, decreased shoot regeneration. Kinetin and 2iP did not influence shoot regeneration at the concentrations tested (1 & 10 M). Addition of benzyladenine, Zeatin, or thidiazuron to the culture medium increased both the percentage of explants that formed shoots and the number of shoots per explant. Thidiazuron was highly effective in stimulating shoot formation at low concentrations (<1 M). At 0.05 M thidiazuron, 95% of the explants produced shoots and about 10 shoots were formed per explant. Compared to TDZ, higher concentrations (10 M) of benzyladenine and Zeatin were required to enhance shoot formation. Upon excision and transfer to B5 medium, regenerated shoots developed into normal rooted plantlets.Abbreviations BA
Benzyladenine
- IAA
Indoleacetic acid
- IBA
Indolebutyric acid
- NAA
Naphthaleneacetic acid
- TDZ
Thidiazuron
- 2ip
Isopentenyladenine 相似文献
18.
In vitro high frequency plant regeneration from hypocotyl and root segments of spinach by organogenesis 总被引:1,自引:0,他引:1
An efficient protocol for spinach (Spinacia oleracea L.) plant regeneration from hypocotyl and root segments was established. When the sub-apical hypocotyl and tip-free root segments were cultured on Murashige & Skoog (1962)-based medium containing high concentrations of indole-3-acetic acid (85.62 M) and gibberellic acid (100 M), more than 75% and 90% of the hypocotyl and root explants, respectively, formed shoots. After elongation, more than 92% of the shoots rooted on medium supplemented with 2.85–5.71 M of indole-3-acetic acid. More than 70% of rooted plantlets survived in soil and were fertile. Significant interactions between growth regulator combinations, explant types and environmental conditions on shoot initiation, development and rooting were discussed.Abbreviations BA
benzyladenine
- BM
Murashige & Skoog basal medium
- B5
Gamborg et al. medium (1968)
- 2,4-d
2,4-dichlorophenoxyacetic acid
- 2ip
isopentenyladenine
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- MS
Murashige & Skoog medium (1962)
- NAA
naphthaleneacetic acid
- HS
hypocotyl segments
- RSS
root segments of seedlings
- RSV
foot segments of in vitro plantlets 相似文献
19.
紫穗槐的离体快速繁殖 总被引:5,自引:0,他引:5
以子叶节为外植体,建立起了紫穗槐的快速离体再生系统.经过四周的培养,在附加8mg·L-16-BA的MS培养基上能够获得再生频率为100%,平均每个外植体5.21个芽点的高效再生植株.以再生植株的茎节为外植体所进行的继代能够在相同的培养基上连续的产生新的不定芽,但芽点数要少于起始培养.经过3周的培养,有82.53%切下的再生茎段能够在含2.0mg·L-1IAA的MS培养基上生根.在所有进行分析过的再生植株中,它们的染色体数目都没有发生变异(2n=40).经过练苗以后,再生植株成功地定植于土壤当中并展示了一致的外部形态和生长特性. 相似文献
20.
Charleson R. Poovaiah Stephen C. Weller Matthew A. Jenks 《In vitro cellular & developmental biology. Plant》2006,42(4):354-358
Summary The effect of different cytokinins on in vitro adventitious shoot regeneration from internodal explants of Menthaxgracilis Sole (scoth spearmint) was investigated. Murashige and Skoog (MS) medium containing 100 mg l−1 myo-inositol, 0.4 mg l−1 thiamine-HCl, 2.0% (w/v) sucrose, 10% (v/v) coconut water and supplemented with 4.5 μM thidiazuron (TDZ) was effective in inducing adventitious shoot formation from callus. The greatest percentage of explants
with shoots (85%) with the highest mean number of shoots per explant (29) was obtained with explants from the 1st and the
2nd internodes from 2-wk-old stock plants growing on a medium containing MS basal salts, 2% sucrose, 100 mg l−1 myo-inositol, 0.4 mg l−1 thiamine-HCl, at TDZ 4.5 μM and 10% (v/v) coconut water and solidified with 0.2% (w/v) phytagel. The regenerated shoots rooted on a medium containing
MS basal salts, 100 mg l−1 myo-inositol, 0.4 mg l−1 thiamine-HCl, 2.0% sucrose, and 0.054 μM naphthalene acetic acid (NAA). Micropropagated plantlets were transplanted into soil and acclimated to greenhouse conditions.
This is the first report describing adventitious shoot regeneration of scotch spearmint. 相似文献