几种药剂及施药方式对稻纵卷叶螟的防治效果

对稻纵卷叶螟Cnaphalocrocis medinalis Guen e高龄幼虫防治药剂31%氟虫腈.三唑磷ME和当地几种常用药剂及不同施药方法对防治效果影响进行试验。结果表明:(1)毒死蜱(48%乐斯本EC)有较好速效性,药后1 d可达理想防效,三唑磷与氟出腈、氟铃脲复配的药剂(31%氟虫腈.三唑磷ME、21%氟虫腈.三唑磷EC、15%氟铃脲.三唑磷EC)药后3d才能达到理想防效;(2)药后3d,各处理均达到理想防效,防效依次为31%氟虫腈.三唑磷ME1.5L/hm2>31%氟虫腈.三唑磷ME1.05L/hm2>21%氟虫腈.三唑磷EC1.5L/hm2>48%毒死蜱EC1.5L/hm2>15%氟虫腈.三唑磷EC1.5L/hm2>15%阿维菌素.毒死蜱EC1.5L/hm2;(3)分虫龄防效统计,药后3d供试药剂对1龄以下(施药时虫龄,后同)幼虫防效均在91%以上,对2~3龄幼虫,31%氟虫腈.三唑磷ME保持90%以上的防效,其它处理防效明显下降,对4龄以上高龄幼虫,31%氟虫腈.三唑磷1.5L/hm2细喷雾处理防效仍达95.6%,其它处理防效很低。(4)细喷雾可提高31%氟虫腈.三唑磷ME对稻纵卷叶螟的防治效果,尤其是对高龄幼虫,与工农16型1.8mm孔径喷片的常规喷雾相比,1.2mm孔径喷片细喷雾处理药后3 d防效提高5.5%,东方红18型弥雾处理防效提高7.8%,其中,弥雾处理对1~2龄幼虫防效提高4.7%,对4~5龄幼虫防效提高了20.8%。     

miRNA-155对氟虫腈作用下斑马鱼ZF4细胞存活的影响

microRNA（miRNA）是一类长约20～24 nt的非编码单链小分子RNA. 环境毒理学研究表明,当生物暴露于环境化学物质时,会引起相关miRNA表达发生变化,进而调节基因的表达. 农药氟虫腈是一种苯基吡唑类杀虫剂,能影响斑马鱼中miR-155的正常表达,但尚未见异位表达的miR-155对斑马鱼细胞影响的研究. 本研究明确了异位表达的miR-155对氟虫腈作用下斑马鱼胚胎细胞ZF4存活的影响. 结果显示,氟虫腈处理ZF4细胞72 h的IC50值为39.05 μmol/L,瞬时转染miR-155 mimic,显著降低了ZF4细胞氟虫腈暴露的存活率.同时real-time PCR结果显示,其潜在靶基因cyb561d2表达显著降低;而转染miR-155 inhibitor,抑制细胞内源性的miR-155则可显著增强ZF4细胞抵抗氟虫腈细胞毒性的能力,同时cyb561d2表达则显著增高.以上结果表明,miR-155参与氟虫腈毒理学效应,其表达量的高低可以影响氟虫腈作用下ZF4细胞的存活率,而其可能的途径之一是通过潜在靶基因cyb561d2的表达调控来实现. 因此,miR-155可作为氟虫腈环境毒性的潜在的生物标志物,有望成为致癌环境化学物质对生物体作用机制研究的新途径.     

杀虫剂对稻纵卷叶螟的室内毒力及田间药效

分别测定了8种杀虫剂对稻纵卷叶螟的室内毒力及田间防效,结果表明:8种杀虫剂对稻纵卷叶螟的LC50为0.671-10171.07mg.L-1,毒力由高到低的顺序为:甲氨基阿维菌素苯甲酸盐>阿维菌素(B1)>氟虫腈>氟铃脲>丙溴磷>毒死蜱>三唑磷>杀虫单。甲氨基阿维菌素苯甲酸盐、阿维菌素(B1)、氟虫腈、氟铃脲对稻纵卷叶螟的毒力分别为杀虫单的15180.7、5619.3、1611.9、997.2倍。在推荐剂量下,氟虫腈、丙溴磷、毒死蜱对稻纵卷叶螟防治效果较好,防效分别达86.11%、84.73%、83.37%。     

羧酸酯酶介导的小菜蛾对氟虫腈的抗性

【目的】羧酸酯酶（carboxylesterases, CarEs）是昆虫重要的解毒代谢酶之一,可以介导靶标昆虫对多种杀虫剂的代谢抗性。本研究检测了羧酸酯酶对小菜蛾Plutella xylostella 抗药性的介导功能,旨在阐明羧酸酯酶在小菜蛾代谢解毒中的生理生化和分子机理。【方法】采用点滴法测定氟虫腈对小菜蛾敏感种群和抗氟虫腈种群的毒力,以及羧酸酯酶抑制剂磷酸三苯酯（triphenyl phosphate, TPP）对氟虫腈的增效作用;以LC₃₀和LC₅₀浓度的氟虫腈处理抗性小菜蛾,测定药剂处理后CarEs酶活性的变化;利用qRT-PCR技术分析Pxae22和Pxae31两个基因在小菜蛾不同发育阶段、组织和种群的表达模式;利用dsRNA干扰Pxae22和Pxae31后观察基因的表达变化和小菜蛾3龄幼虫对药剂敏感性的变化。【结果】TPP可以削弱小菜蛾3龄幼虫对氟虫腈的抗性,增效倍数约为6倍;使用较低剂量（LC₃₀和LC₅₀）氟虫腈处理小菜蛾3龄幼虫后,处理组CarEs比活力明显高于对照,提示氟虫腈对小菜蛾CarEs活性具有诱导作用。对羧酸酯酶基因Pxae22和Pxae31在小菜蛾不同发育阶段、4龄幼虫不同组织和不同种群3龄幼虫中的表达模式分析发现,这两个基因在小菜蛾4龄幼虫中的表达量最高;在4龄幼虫中以中肠组织中的表达量较高,头、表皮、脂肪体中的表达量很低; 抗性种群中的表达量显著高于敏感种群。通过干扰 Pxae22和 Pxae31后的qRT-PCR验证,两个基因的表达量均显著降低,进一步的氟虫腈毒力测定发现,干扰P xae22和 Pxae31后的小菜蛾3龄幼虫对氟虫腈的敏感性分别增加了1.63倍和1.73倍。【结论】羧酸酯酶在小菜蛾对氟虫腈解毒代谢中具有重要作用;Pxae22和Pxae31是小菜蛾的两个抗性相关基因,其表达水平的变化直接影响小菜蛾对氟虫腈的敏感性。     

溴虫腈对家蚕和桑树害虫的毒力比较

为了比较溴虫腈在家蚕Bombyx mori和桑树害虫间的选择性毒力,采用食下毒叶法测定了溴虫腈、二嗪磷、敌敌畏、辛硫磷和三唑磷5种杀虫剂对家蚕的毒性;用浸叶法测定了5种杀虫剂对桑尺蠖Phthonandria atrilineata Butler、桑螟Diaphania pyloalis Walker和桑毛虫Porthesia xanthocampa Dyer 的毒力;在桑园喷施溴虫腈检测了其在桑树上对家蚕的残留毒性期;通过食下毒叶法系统研究了溴虫腈对家蚕生长发育的影响。结果表明,二嗪磷、敌敌畏、辛硫磷和三唑磷72 h对家蚕的毒性倍数分别大于溴虫腈的15.5、93.3、154 .0和188.5倍;溴虫腈、三唑磷、辛硫磷和敌敌畏48 h相对于二嗪磷对桑尺蠖的毒力倍数分别是49.1、3.2、2.3和1.4倍,对桑螟的毒力倍数分别是79.4、3.6、2.4和1.8倍,对桑毛虫的毒力倍数分别为67.2、3.2、2.2和1.7倍;对桑树喷施100、50、25和12.5 mg/L 溴虫腈,桑叶的残留毒性期分别为3、0、0和0天;用溴虫腈100、50、25和12.5 mg/L 处理的桑叶饲喂家蚕后,存活幼虫的历期、眠蚕体重、熟蚕体重、全茧量、茧层量、蛹重和化蛹率与对照相比均无显著性差异。据此认为溴虫腈是适合防治桑园害虫又对家蚕较安全的药剂。     

microRNAs在大鼠急性心肌梗死过程的表达变化

目的研究分析microRNAs（miRNAs,miRs）在大鼠急性心肌梗死（acutemyocardialinfarction,AMI）心肌组织的梗死区与非梗死区的表达变化,为防治AMI提供基础数据。方法选择雄性sD大鼠为研究对象,建立结扎左冠状动脉造成的急性心肌梗死模型,取建模后6h的梗死区与非梗死区的心肌组织进行芯片检测．确定其中表达变化显著的miRNAs;最后进行定量逆转录聚合酶链反应（qRT—PCR）,定量分析梗死区与非梗死区心肌组织中miRNAs的表达。结果AMI大鼠心肌组织中,芯片筛选出在AMI前后发生显著波动的miRNAs,与非梗死区相比,梗死区心肌组织中有26个miRNAs表达发生了显著变化,其中19个miRNA表达下调,7个miRNA表达上调。结论在AMI后的心肌组织的梗死部位与非梗死部位miRNAs的表达是有显著差异的,这对AMI阶段心肌保护的救治具有重要意义。     

MicroRNA与肺癌发生、诊断及治疗

微小RNA(miRNAs)是一大类小的非编码RNA,它通过与靶mRNA 3′非翻译区部分互补配对来调节特定基因的表达。近来研究表明,miRNA可作为癌基因或抑癌基因在肺癌发生发展过程中起重要作用。比较癌组织和非癌组织中miRNA表达谱的差异可筛选出部分miRNA分子作为肺癌诊断和预后判断的潜在生物标记。调节具有致癌或抑癌功能的miRNA表达可能成为肺癌治疗新方法,而结合传统放化疗及其敏感性miRNA标志也为肺癌治疗研究提供了新的策略。该文对miRNA在肺癌发生与发展、基因诊断和治疗中的作用做一综述。     

HIV-1感染者中全血miRNA表达谱的变化

初步了解HIV-1感染者全血中miRNA表达谱的变化。使用Taqman低密度miRNA表达谱芯片,分别检测10例HIV-1感染者和10例未感染者全血样本中754条miRNA的表达情况。通过BRB分析软件对样本中miR-NAs的表达情况进行比较,筛选出差异表达的miRNA。使用DIANA在线工具预测差异表达miRNAs的靶基因和细胞功能。筛选结果显示有56条miRNAs显著差异表达(P<0.001),其中有49条miRNAs在感染者中下调,7条miRNAs的表达上调。差异表达miRNAs的靶基因涉及的生物学功能相对集中,主要富集在MAPK、TGF-be-ta、Wnt等信号通路。提示:HIV-1感染引起全血中部分miRNAs表达情况的改变。56条显著差异表达的miRNA可能有着重要的作用,对这些miRNA的进一步研究有望发现新的与致病机制相关的关键分子和HIV-1感染诊断潜在的标志物。     

五种植物精油对氟虫腈的增效与渗透促进作用

采用点滴法测定了花椒Zanthoxylum bungeanum Maxim,艾蒿Artemisia grgyi Levl. Et Vant,马尾松针Pinus massoniana Lamb,侧柏Platycladus orientalis(L.)Franco,黄蒿Artemisia annua L. 5种植物精油与氟虫腈联用对小菜蛾2龄幼虫的触杀毒力.研究结果表明,当植物精油浓度为1μL·mL-1时对氟虫腈的LC50均无显著增效作用,当上述精油浓度含量为10μL·mL-1时,艾蒿与侧柏氟虫腈能显著提高氟虫腈对小菜蛾幼虫的毒杀效果;致死中时(LT50)测定结果表明,当植物精油浓度为lpLL·mL-1时,仅艾蒿精油能缩短氟虫腈对小菜蛾幼虫的LT50,但当植物精油浓度为10μL·mL-1时,所有精油均能缩短氟虫腈对小菜蛾幼虫LT50;松针精油和侧柏精油能提高氟虫腈透皮吸收量,其余精油对氟虫腈没有显著促进渗透作用.     

苎麻纤维不同发育时期差异表达miRNAs的表达谱分析

miRNA在植物生长发育过程中起着重要的调控作用.本研究从苎麻纤维伸长期和胞壁加厚、端壁溶合期的中苎1号苎麻韧皮组织为材料的miRNA芯片中筛选出3个差异表达的miRNAs:miR1450、miR156h和miR172b1.利用半定量RT-PCR技术对3个差异表达miRNAs在4个苎麻纤维发育时期(苎麻纤维分生期,纤维伸长期,胞壁加厚端壁融合期和纤维成熟期)的韧皮组织以及花、叶、根和地下茎等组织和器官中的表达情况进行研究.结果表明供试的3个miRNA在苎麻纤维伸长期和胞壁加厚端壁融合期的表达量均为差异表达,进一步验证了芯片结果,其中mi1450在纤维成熟期表达量最高,在纤维伸长期的表达量最低;mi156h在花、地下茎、根和叶中的表达量显著高于4个苎麻纤维发育时期;mi172b1在纤维分生期的表达量最高,在花中的表达量最低.本研究建立了苎麻miRNA的茎环RT-PCR检测体系,为进一步研究miRNA在苎麻纤维发育过程中的作用奠定了基础.     

应用双荧光报告系统检测斑马鱼microRNA的动态表达

microRNA(miRNA)是一类细胞内源表达的小分子非编码RNA, 主要通过降解靶基因的mRNA或者抑制靶基因的翻译, 在动植物的发育以及其他重要的生理过程中起调控作用。miRNA的功能跟它的表达位置与时间密切相关, 但是目前尚缺乏一个能够在活体与个体水平稳定、持续地实时观察miRNA动态表达的方法。文章以斑马鱼为模式, 建立了一个双荧光报告系统(我们称之为miRNA Tracer), 用于在斑马鱼整体胚胎中追踪特定miRNA的表达谱及动态变化过程。该系统以Tol2转座子为基础, 采用来自斑马鱼hsp70基因的热激启动子分别驱动eGFP和mRFP1荧光报告基因, 同时在其中一个报告基因的3′-UTR区连接待测miRNA的互补序列, 构成Tracer质粒。该互补序列与斑马鱼胚胎中相应的内源miRNA结合后能够使对应报告基因的荧光信号强度减弱, 通过比较两个报告基因在表达谱上的差异辨别miRNA的表达区域, 检测斑马鱼胚胎中miRNA起作用的位置和时间。文章选择在肌肉系统特异表达的miR-206以及在神经系统特异表达的miR-219, 分别在显微注射瞬时表达和转基因稳定整合等两个层次上验证了上述Tracer系统。结果表明, 所用的方法能够如实地在单细胞水平和整体水平检测到目标miRNA的时空表达动态变化。miRNA Tracer系统为在斑马鱼发育过程中对miRNA进行活体、实时的时空定位提供了一个独特而有效的方法, 也为对miRNA进行功能与作用机制等更深入的研究奠定了基础。

补充资料

s219mRFP1-dF转基因胚胎的3-D图像 [视频]     

Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development

Several vertebrate microRNAs (miRNAs) have been implicated in cellular processes such as muscle differentiation, synapse function, and insulin secretion. In addition, analysis of Dicer null mutants has shown that miRNAs play a role in tissue morphogenesis. Nonetheless, only a few loss-of-function phenotypes for individual miRNAs have been described to date. Here, we introduce a quick and versatile method to interfere with miRNA function during zebrafish embryonic development. Morpholino oligonucleotides targeting the mature miRNA or the miRNA precursor specifically and temporally knock down miRNAs. Morpholinos can block processing of the primary miRNA (pri-miRNA) or the pre-miRNA, and they can inhibit the activity of the mature miRNA. We used this strategy to knock down 13 miRNAs conserved between zebrafish and mammals. For most miRNAs, this does not result in visible defects, but knockdown of miR-375 causes defects in the morphology of the pancreatic islet. Although the islet is still intact at 24 hours postfertilization, in later stages the islet cells become scattered. This phenotype can be recapitulated by independent control morpholinos targeting other sequences in the miR-375 precursor, excluding off-target effects as cause of the phenotype. The aberrant formation of the endocrine pancreas, caused by miR-375 knockdown, is one of the first loss-of-function phenotypes for an individual miRNA in vertebrate development. The miRNA knockdown strategy presented here will be widely used to unravel miRNA function in zebrafish.     

The crucial role and regulations of miRNAs in zebrafish development

To comprehend the events during developmental biology, fundamental knowledge about the basic machinery of regulation is a prerequisite. MicroRNA (miRNAs) act as regulators in most of the biological processes and recently, it has been concluded that miRNAs can act as modulatory factors even during developmental process from lower to higher animal. Zebrafish, because of its favorable attributes like tiny size, transparent embryo, and rapid external embryonic development, has gained a preferable status among all other available experimental animal models. Currently, zebrafish is being utilized for experimental studies related to stem cells, regenerative molecular medicine as well drug discovery. Therefore, it is important to understand precisely about the various miRNAs that controls developmental biology of this vertebrate model. In here, we have discussed about the miRNA-controlled zebrafish developmental stages with a special emphasis on different miRNA families such as miR-430, miR-200, and miR-133. Moreover, we have also reviewed the role of various miRNAs during embryonic and vascular development stages of zebrafish. In addition, efforts have been made to summarize the involvement of miRNAs in the development of different body parts such as the brain, eye, heart, muscle, and fin, etc. In each section, we have tried to fulfill the gaps of zebrafish developmental biology with the help of available knowledge of miRNA research. We hope that precise knowledge about the miRNA-regulated developmental stages of zebrafish may further help the researchers to efficiently utilize this vertebrate model for experimental purpose.     

microRNA在小鼠乳腺不同发育时期差异表达谱及作用

microRNA是一类大小约22个核苷酸的非编码RNA分子,是一种广泛存在的对基因表达进行微调的分子。microRNA可以通过与靶基因mRNA的特定位点结合,抑制该蛋白的合成或诱导该mRNA的降解,从而参与基因的表达调控。一般来源于染色体的非编码区域,由大约70个核苷酸大小的可形成发夹结构的前体经Dicer酶加工而来。这类小RNA在表达上具有组织和时间的特异性,是调节其他功能基因表达的重要调控分子,在生物的生长发育过程中发挥着重要作用。因此,虽然microRNA的研究仅有很短的历史,但已成为基因表达调控研究的热点领域。以中国昆明小鼠不同发育时期的乳腺组织为实验材料,应用芯片技术及荧光定量PCR技术,分析发育不同时期的乳腺组织microRNA差异表达图谱。本文研究发现microRNA在乳腺不同的发育时期表达图谱不同;与青春期、退化期比较,妊娠期、哺乳期有十余种microRNAs表达上调,20余种microRNAs表达下调;microRNAs在乳腺发育和泌乳周期中发挥重要的作用。     

Selective extracellular vesicle-mediated export of an overlapping set of microRNAs from multiple cell types

ABSTRACT: BACKGROUND: MicroRNAs (miRNAs) are a class of small RNA molecules that regulate expression of specific mRNA targets. They can be released from cells, often encapsulated within extracellular vesicles (EVs), and therefore have the potential to mediate intercellular communication. It has been suggested that certain miRNAs may be selectively exported, although the mechanism has yet to be identified. Manipulation of the miRNA content of EVs will be important for future therapeutic applications. We therefore wished to assess which endogenous miRNAs are enriched in EVs and how effectively an overexpressed miRNA would be exported. RESULTS: Small RNA libraries from HEK293T cells and vesicles before or after transfection with a vector for miR-146 overexpression were analysed by deep sequencing. A subset of miRNAs was found to be enriched in EVs; pathway analysis of their predicted target genes suggests a potential role in regulation of endocytosis. RT-qPCR in additional cell types and analysis of publicly available data revealed that many of these miRNAs tend to be widely preferentially exported. Whilst overexpressed miR-146a was highly enriched both in transfected cells and their EVs, the cellular:EV ratios of endogenous miRNAs were not grossly altered. MiR-451 was consistently the most highly exported miRNA in many different cells types. Intriguingly, Argonaute2 (Ago2) is required for miR-451 maturation and knock out of Ago2 has been shown to decrease expression of other preferentially exported miRNAs (eg miR-150 and miR-142-3p). CONCLUSION: The global expression data provided by deep sequencing confirms that specific miRNAs are enriched in EVs released by HEK293T cells. Observation of similar patterns in a range of cell types suggests that a common mechanism for selective miRNA export may exist.     

Members of the miRNA-200 family regulate olfactory neurogenesis

MicroRNAs (miRNAs) are highly expressed in vertebrate neural tissues, but the contribution of specific miRNAs to the development and function of different neuronal populations is still largely unknown. We report that miRNAs are required for terminal differentiation of olfactory precursors in both mouse and zebrafish but are dispensable for proper function of mature olfactory neurons. The repertoire of miRNAs expressed in olfactory tissues contains over 100 distinct miRNAs. A subset, including the miR-200 family, shows high olfactory enrichment and expression patterns consistent with a role during olfactory neurogenesis. Loss of function of the miR-200 family phenocopies the terminal differentiation defect observed in absence of all miRNA activity in olfactory progenitors. Our data support the notion that vertebrate tissue differentiation is controlled by conserved subsets of organ-specific miRNAs in both mouse and zebrafish and provide insights into control mechanisms underlying olfactory differentiation in vertebrates.     

Identification of candidate microRNA biomarkers in renal fibrosis: a meta-analysis of profiling studies

Abstract

The prognostic, diagnostic and therapeutic value of microRNA (miRNA) expression aberrations in renal fibrosis has been studied in recent years. However, the miRNA expression profiling efforts have led to inconsistent results between the studies. The aim of this study was to perform a meta-analysis on the renal fibrosis miRNA expression profiling studies to identify candidate diagnostic biomarkers. We performed comprehensive literature searches in several databases to identify miRNA expression studies of renal fibrosis in animal models and humans. The miRNAs expression data were extracted from 20 included studies, and both miRNA vote-counting strategy and Robust Rank Aggregation method were utilized to identify significant miRNA meta-signatures. The predicted and validated targets of miRNA meta-signature were obtained by using MultiMiR package in 11 databases. Then a gene set enrichment analysis (KEGG, PANTHER pathways and GO processes) were carried out with GeneCodis web tool to recognize pathways that are most strongly influenced by modified expressions of these miRNAs. We recognized in both meta-analysis approaches a significant miRNA meta-signature of five up-regulated (miR-142-3p, miR-223-3p, miR-21-5p, miR-142-5p and miR-214-3p) and two down-regulated (miR-29c-3p and miR-200a-3p) miRNAs. Enrichment analysis confirmed that miRNA meta-signature cooperatively target functionally related genes in signalling and developmental pathways in renal fibrosis. This meta-analysis identified seven highly significant and consistently dysregulated miRNAs from 20 datasets, as the focus of future investigations to discover their potential influence to renal fibrosis and their clinical utility as biomarkers and/or as therapeutic mediators against chronic kidney disease..     

胃癌相关miRNA表达的表观遗传学调控机制

胃癌是人类最常见的肿瘤之一,其发病机制尚不完全清楚.微小RNA(microRNA,miRNA)是一组最近发现的长度为22个核苷酸左右的非编码RNA,具有负性调控基因表达的功能.本文对miRNA在胃癌发生中的作用及其表达调控机制进行综述.不断有文献显示,miRNA在多种肿瘤(包括胃癌)的发生过程中发挥着重要作用.作者和其他研究人员发现,miRNA的表达异常(如:miR-421和miR-21的上调或/和miR-31和miR-218的下调等)与胃癌的发生相关,提示miRNA是胃癌发生的重要因素.目前,miRNA表达的分子机制尚未完全明了.最近研究较清楚地显示,miRNA的表达受到DNA甲基化和组蛋白修饰等机制的调控.这说明,胃癌相关miRNA的表达水平受到表观遗传机制的调控。     

Integrated analyses of zebrafish miRNA and mRNA expression profiles identify miR-29b and miR-223 as potential regulators of optic nerve regeneration

Background

Unlike mammals, zebrafish have the ability to regenerate damaged parts of their central nervous system (CNS) and regain functionality of the affected area. A better understanding of the molecular mechanisms involved in zebrafish regeneration may therefore provide insight into how CNS repair might be induced in mammals. Although many studies have described differences in gene expression in zebrafish during CNS regeneration, the regulatory mechanisms underpinning the differential expression of these genes have not been examined.

Results

We used microarrays to analyse and integrate the mRNA and microRNA (miRNA) expression profiles of zebrafish retina after optic nerve crush to identify potential regulatory mechanisms that underpin central nerve regeneration. Bioinformatic analysis identified 3 miRNAs and 657 mRNAs that were differentially expressed after injury. We then combined inverse correlations between our miRNA expression and mRNA expression, and integrated these findings with target predictions from TargetScan Fish to identify putative miRNA-gene target pairs. We focused on two over-expressed miRNAs (miR-29b and miR-223), and functionally validated seven of their predicted gene targets using RT-qPCR and luciferase assays to confirm miRNA-mRNA binding. Gene ontology analysis placed the miRNA-regulated genes (eva1a, layna, nefmb, ina, si:ch211-51a6.2, smoc1, sb:cb252) in key biological processes that included cell survival/apoptosis, ECM-cytoskeleton signaling, and heparan sulfate proteoglycan binding,

Conclusion

Our results suggest a key role for miR-29b and miR-223 in zebrafish regeneration. The identification of miRNA regulation in a zebrafish injury model provides a framework for future studies in which to investigate not only the cellular processes required for CNS regeneration, but also how these mechanisms might be regulated to promote successful repair and return of function in the injured mammalian brain.

Electronic supplementary material

The online version of this article (doi:10.1186/s12864-015-1772-1) contains supplementary material, which is available to authorized users.