Risk of dietary magnesium deficiency is low in most African countries based on food supply data

Background

Dietary mineral deficiencies are widespread in Africa. Our previous studies in Malawi revealed population-level shortfalls in dietary calcium and selenium supply but adequate dietary magnesium (Mg) supply. Here we examine dietary Mg supply throughout Africa.

Methods

Food supply data from 1961 to 2007 were compiled using Food and Agriculture Organization (FAO) Food Balance Sheets (FBSs). Magnesium supply was estimated for each country using regional food Mg composition tables.

Results

Mean Mg supply in 2007 was 649 mg capita ^?1 d^?1, ranging from 188 mg d^?1 in Eritrea to 1,828 mg d^?1 in Burkina Faso. Magnesium supply was greater in West Africa than in other regions, was dominated by sorghum, maize and wheat and was correlated with calorie supply. The World Health Organization (WHO) Estimated Average Requirement (EAR) for Mg (217 mg capita ^?1 d^?1 for adult males) was exceeded in most countries. Using the EAR cut-point method, the risk of dietary Mg deficiency in Africa is <4 % and unlikely to be a major problem, assuming access to sufficient food and that phytic acid does not compromise Mg absorption.

Conclusions

Estimating Mg supply is highly sensitive to concentration data available for the primary staple crops. Given that soil factors profoundly affect crop Mg concentration, there is a need to increase the spatial resolution of food composition tables for the staple crops.     

The significance of magnesium for crop quality

Background

The quality of agricultural and horticultural products and its modulation by fertilization has increasingly received attention. However, whereas the importance of magnesium (Mg) as an essential plant nutrient is well established, the impact of Mg nutrition on quality parameters has only been rarely addressed.

Scope

This review aims at evaluating the available knowledge on the influence of Mg on produce quality. A short discussion on the term quality as used in this review is followed by an overview of the various functions of Mg in plant metabolism in relation to quality aspects. Finally, the available literature on Mg-associated effects on crop quality is critically surveyed. The question whether Mg application beyond yield optimum further improves crop quality is specifically addressed.

Conclusion

Increasing Mg supply on Mg-deficient sites tends to increase the quality of agricultural crops, particularly when the formation of quality traits is dependent on Mg-driven photosynthesis and assimilate translocation within the plant. In fruits and vegetables, ratios of Mg to other nutrients like Ca and K were shown to be a more reliable indicator of the quality response than the Mg status alone. Moreover, it is concluded that Mg doses beyond those required for maximum yield rarely induce a further improvement of produce quality.     

IJ_Rhizo: an open-source software to measure scanned images of root samples

Background and aims

This paper provides an overview of the measuring capabilities of IJ_Rhizo, an ImageJ macro that measures scanned images of washed root samples. IJ_Rhizo is open-source, platform-independent and offers a simple graphic user interface (GUI) for a main audience of non-programmer scientists. Being open-source based, it is also fully modifiable to accommodate the specific needs of the more computer-literate users. A comparison of IJ_Rhizo’s performance with that of the widely used commercial package WinRHIZO? is discussed.

Methods

We compared IJ_Rhizo’s performance with that of the commercial package WinRHIZO? using two sets of images, one comprising test-line images, the second consisting of images of root samples collected in the field. IJ_Rhizo and WinRHIZO? estimates were compared by means of correlation and regression analysis.

Results

IJ_Rhizo “Kimura” and WinRHIZO? “Tennant” were the length estimates that were best linearly correlated with each other. Correlation between average root diameter estimates was weaker, due to the sensitivity of this parameter to thresholding and filtering of image background noise.

Conclusions

Overall, IJ_Rhizo offers new opportunities for researchers who cannot afford the cost of commercial software packages to carry out automated measurement of scanned images of root samples, without sacrificing accuracy.     

Edaphic influences of ophiolitic substrates on vegetation in the Western Italian Alps

Background and aims

Soils derived from serpentinite (serpentine soils) often have low macronutrient concentrations, exceedingly low Ca:Mg molar ratios and high heavy metal concentrations, typically resulting in sparse vegetative cover. This combined suite of edaphic stresses is referred to as the “serpentine syndrome.” Although several plant community-level studies have been conducted to identify the most important edaphic factor limiting plant growth on serpentine, the primary factor identified has often varied by plant community and local climate. Few studies to date have been conducted in serpentine plant communities of alpine or boreal climates. The goal of our study was to determine the primary limiting edaphic factors on plant community species composition and productivity (cover) in the alpine and boreal climate of the Western Alps, Italy.

Methods

Soil properties and vegetation composition were analyzed for several sites underlain by serpentinite, gabbro, and calc-schist substrates and correlated using direct and indirect statistical methods.

Results

Boreal forest soils were well-developed and tended to have low pH throughout the soil profile resulting in high Ni availability. Alpine soils, in comparison, were less developed. The distinct serpentine plant communities of the Western Alps are most strongly correlated with high levels of bioavailable Ni associated with low soil pH. Other factors such as macronutrient deficiency, low Ca:Mg molar ratio and drought appear to be less important.

Conclusions

The strong ecological influence of Ni is caused by environmental conditions which increase metal mobilization.     

Predictors of mortality of patients newly diagnosed with clinical type 2 diabetes: a 5-year follow up study

Background

At diabetes diagnosis major decisions about life-style changes and treatments are made based on characteristics measured shortly after diagnosis. The predictive value for mortality of these early characteristics is widely unknown. We examined the predictive value of patient characteristics measured shortly after diabetes diagnosis for 5-year all-cause and cardiovascular mortality with special reference to self-rated general health.

Methods

Data were from a population-based sample of 1,323 persons newly diagnosed with clinical diabetes and aged 40 years or over. Possible predictors of mortality were investigated in Cox regression models.

Results

Multivariately patients who rated their health less than excellent experienced increased all-cause and cardiovascular mortality. These end-points also increased with sedentary life-style, relatively young age at diagnosis and presence of cardiovascular disease (CVD) at diagnosis. Further predictors of all-cause mortality were male sex, low body mass index and cancer, while cardiovascular mortality increased with urinary albumin concentration.

Conclusions

We found that patients who rated their health as less than excellent had increased 5-year mortality, similar to that of patients with prevalent CVD, even when biochemical, clinical and life-style variables were controlled for. This finding could motivate doctors to discuss perceptions of health with newly diagnosed diabetic patients and be attentive to patients with suboptimal health ratings. Our findings also confirm that life-style changes and optimizing treatment are particularly relevant for relatively young and inactive patients and those who already have CVD or (micro)albuminuria at the time of diabetes diagnosis.     

On the uncanny capabilities of consequential LCA

Purpose

Plevin et al. (2014) reviewed relevant life cycle assessment (LCA) studies for biofuels and argued that the use of attributional LCA (ALCA) for estimating the benefits of biofuel policy is misleading. While we agree with the authors on many points, we found that some of the arguments by the authors were not presented fairly and that a number of specific points warrant additional comment. The main objective of this commentary is to examine the authors’ comparative statements between consequential LCA (CLCA) and ALCA.

Methods

We examined the notion that the LCA world is divided into CLCA and ALCA. In addition, we evaluated the authors’ notion of “wrong” models.

Results

We found that the authors were comparing an idealized, hypothetical CLCA with average (or less than average), real-life ALCAs. Therefore, we found that the comparison alone cannot serve as the basis for endorsing real-life CLCAs for biofuel policy. We also showed that there are many LCA studies that do not belong to either of the two approaches distinguished by the authors. Furthermore, we found that the authors’ notion of “wrong” models misses the essence of modeling and reveals the authors’ unwarranted confidence in certain modeling approaches.

Conclusions

Dividing the LCA world into CLCAs and ALCAs overlooks the studies in between and hampers a constructive dialog about the creative use of modeling frameworks. Unreasonable confidence in certain modeling approaches based on their “conceptual” superiority does not help support “robust decision making” that should ultimately land itself on the ground.     

Life cycle carbon footprint measurement of Portland cement and ready mix concrete for a city with local scarcity of resources like Hong Kong

Purpose

The construction sector was the second largest contributor of Hong Kong carbon emissions, and 85 % of the emission from construction was external in nature. The carbon footprint embodied in each building construction material varies considerably under different conditions. This study aims to quantify the embodied carbon footprint of building construction materials used in Hong Kong with the consideration of local factors and to investigate how the region-specific characteristic would affect the result.

Methods

A “cradle-to-site” system boundary was used, including raw material extraction, manufacturing, and transport until the material reaches the construction site. Data were collected from manufacturers in local and nearby regions. Portland cement and ready mix concrete were selected as examples in this study to demonstrate the calculation.

Results and discussion

It is indicated that for cement, decomposition of limestone contributes the largest to the total greenhouse gas emission over the life cycle, followed by fuel combustion. The surveyed cement plant performs at an average level in manufacture, but the import of raw materials increases the total emissions. For concrete, the major contributor is cement manufacturing. Comparison with other databases reveals that there is room for improvement in carbon reduction of the surveyed plants. The “cradle-to-site” results on cement and concrete show no significant difference from the “cradle-to-gate” results.

Conclusions

Hong Kong’s dependency on imports increases the carbon footprint of locally used building construction materials. The presented methodology can be modified and extended to other materials, thereby helping lower the carbon footprint of construction activities by providing a benchmark for selecting green materials.     

L'orgasme de l'homme adulte

We provide data on several aspects of sexual behaviour: masturbation, age at first intercourse, sexual practices during first intercourse, homosexuality and heterosexuality, sexual practices over a lifetime, sexual activity in the past twelve months, frequency of sexual intercourse, last sexual intercourse, achieving orgasm (through vaginal penetration, masturbation by partners, fellatio and cunnilingus), sexual dysfunctions. Data come from recent national sex surveys in: Great Britain (1990–91; n=18.876), Finland (1991–92; n=2250), the United States (1992; n="3432) and especially from the ACSF survey (n=20.055), the French national telephone survey on sexual behaviour conducted between September 1991 and February 1992. Some results about the prevalence of sexual dysfunctions in sexually active men 18 to 69 years old, in France (in %):

- “You do not have an erection (impotence)”

- “often”: 7%, “sometimes”: 12%

-“You ejaculate too early, even before entering you partner” “often”: 5%; “sometimes”: 16%

- “You ejaculate too soon, upon or shortly after entering your partner” “often”: 10%; “sometimes”: 27%

- “It takes you too long to ejaculate once you have entered your partner” “often”: 4%; “sometimes”: 16%

- “You fail to ejaculate at all” “often”: 2%; “sometimes”: 5%

- “You don't have an orgasm” “often”: 7%; “sometimes”: 7%

- “You feel no or insufficient sexual desire” “often”: 3%; “sometimes”: 16%.

     

Effects of mother-infant skin-to-skin contact on severe latch-on problems in older infants: a randomized trial

Background

Infants with latch-on problems cause stress for parents and staff, often resulting in early termination of breastfeeding. Healthy newborns experiencing skin-to-skin contact at birth are pre-programmed to find the mother’s breast. This study investigates if skin-to-skin contact between mothers with older infants having severe latching on problems would resolve the problem.

Methods

Mother-infant pairs with severe latch-on problems, that were not resolved during screening procedures at two maternity hospitals in Stockholm 1998–2004, were randomly assigned to skin-to-skin contact (experimental group) or not (control group) during breastfeeding. Breastfeeding counseling was given to both groups according to a standard model. Participants were unaware of their treatment group. Objectives were to compare treatment groups concerning the proportion of infants regularly latching on, the time from intervention to regular latching on and maternal emotions and pain before and during breastfeeding.

Results

On hundred and three mother-infant pairs with severe latch-on problems 1–16 weeks postpartum were randomly assigned and analyzed. There was no significant difference between the groups in the proportion of infants starting regular latching-on (75% experimental group, vs. 86% control group). Experimental group infants, who latched on, had a significantly shorter median time from start of intervention to regular latching on than control infants, 2.0 weeks (Q₁ = 1.0, Q₃ = 3.7) vs. 4.7 weeks (Q₁ = 2.0, Q₃ = 8.0), (p-value = 0.020). However, more infants in the experimental group (94%), with a history of “strong reaction” during “hands-on latch intervention”, latched-on within 3 weeks compared to 33% in the control infants (Fisher Exact test p-value = 0.0001). Mothers in the experimental group (n = 53) had a more positive breastfeeding experience according to the Breastfeeding Emotional Scale during the intervention than mothers in the control group (n = 50) (p-value = 0.022).

Conclusions

Skin-to-skin contact during breastfeeding seems to immediately enhance maternal positive feelings and shorten the time it takes to resolve severe latch-on problems in the infants who started to latch. An underlying mechanism may be that skin-to-skin contact with the mother during breastfeeding may calm infants with earlier strong reaction to “hands on latch intervention” and relieve the stress which may have blocked the infant’s inborn biological program to find the breast and latch on.

Trial registration

Karolinska Clinical Trial Registration number CT20100055     

A prospective cohort study of stroke characteristics,care, and mortality in a hospital stroke registry in Vietnam

Background

As low and middle-income countries such as Vietnam experience the health transition from infectious to chronic diseases, the morbidity and mortality from stroke will rise. In line with the recommendation of the Institute of Medicine’s report on “Promoting Cardiovascular Health in the Developing World” to “improve local data”, we sought to investigate patient characteristics and clinical predictors of mortality among stroke inpatients at Da Nang Hospital in Vietnam.

Methods

A stroke registry was developed and implemented at Da Nang Hospital utilizing the World Health Organization’s Stroke STEPS instrument for data collection.

Results

754 patients were hospitalized for stroke from March 2010 through February 2011 and admitted to either the intensive care unit or cardiology ward. Mean age was 65 years, and 39% were female. Nearly 50% of strokes were hemorrhagic. At 28-day follow-up, 51.0% of patients with hemorrhagic stroke died whereas 20.3% of patients with ischemic stroke died. A number of factors were independently associated with 28-day mortality; the two strongest independent predictors were depressed level of consciousness on presentation and hemorrhagic stroke type. While virtually all patients completed a CT during the admission, evidence-based processes of care such as anti-thrombotic therapy and carotid ultrasound for ischemic stroke patients were underutilized.

Conclusions

This cohort study highlights the high mortality due in part to the large proportion of hemorrhagic strokes in Vietnam. Lack of hypertension awareness and standards of care exacerbated clinical outcomes. Numerous opportunities for simple, inexpensive interventions to improve outcomes or reduce recurrent stroke have been identified.

     

Vehicle manufacturing water use and consumption: an analysis based on data in automotive manufacturers’ sustainability reports

Purpose

The goal of this study is to develop an estimate of water use and consumption in automotive manufacturing to enhance the data quality of vehicle life cycle assessments that include life cycle water impacts. A benchmark is developed to compare water resources across manufacturing and nonproduction-related manufacturing processes, including an indication whether indirect water consumption due to electricity generation is significant.

Methods

Data from 12 original equipment manufacturers’ (OEM’s) sustainability reports are examined for the years 2006 to 2010. Distinctions are made between “water use” and “water consumption.” These factors are divided by total reported production to develop use and consumption values in cubic meter/vehicle for comparison. Additionally, total energy consumption is converted to indirect water consumption based on the water consumed in the generation of electricity for the electricity grid mix.

Results and discussion

Excluding outliers, average direct water use is 5.20 and 5.95 m³/vehicle for manufacturing and company-wide activities, respectively, with corresponding standard deviations of 1.42 and 1.20 m³/vehicle. Average direct water consumption was calculated to be 1.25 and 4.29 m³/vehicle for manufacturing and company-wide activities, respectively, with corresponding standard deviations of 0.52 and 1.56 m³/vehicle. Average indirect water consumption due to electricity consumption is found to be 2.21 m³/vehicle. Variability arises through different understandings on the words “consumption” and “use,” reporting continuity between years and in classification of data as it relates to manufacturing, nonmanufacturing, or company-wide activities.

Conclusions

These water values show that needs vary widely across OEMs. Additionally, the magnitude of the indirect water consumption results indicates that OEMs should focus on both indirect and direct water consumption to reduce their overall water footprint. The results also highlight the potential for significance and variability in indirect water consumption, in particular for “cradle-to-gate” type of impact assessments, dependent on electricity generation water consumption assumptions. It is hoped that with the introduction of water reporting standards like the International Organization of Standardization 14046, manufacturers will provide a more comprehensive summary of their water use and consumption in the future.     

Relationships between serpentine soils and vegetation in a xeric inner-Alpine environment

Aims

In serpentinitic areas non-endemic plants suffer from the serpentine syndrome, due to high Ni and Mg concentrations, low nutrients and Ca/Mg ratio. We evaluated the environment-soil-vegetation relationships in a xeric inner-alpine area (NW Italy), where the inhibited pedogenesis should enhance parent material influences on vegetation.

Methods

Site conditions, topsoil properties, plant associations and species on and off serpentinite were statistically associated (51 sites).

Results

Serpentine soils had higher Mg and Ni concentrations, but did not differ from non-serpentine ones in nutrient contents. The 15 vegetation clusters often showed substrate specificity. Two components of the Canonical Analysis of Principal Coordinates, respectively related to Mg and to Ni and heat load, identified serpentine vegetation. Random Forests showed that several species were positively correlated with Ni and/or Ca/Mg or Mg, some were negatively associated with high Ni, Mg excess affected only few species. Considering only serpentine sites, nutrients and microclimate were most important.

Conclusions

Ni excess most often precludes the presence of plant species on serpentinite, while an exclusion due to Mg is rarer. Endemic species are mostly adapted to both factors. Nutrient scarcity was not specific of serpentine soils in the considered environment. Considering only serpentine sites, nutrient and microclimatic gradients drove vegetation variability.     

The capability of endophytic fungi for production of hemicellulases and related enzymes

Background

Currently, the step-wise integration of tet-dependent transactivator and tet-responsive expression unit is considered to be the most promising tool to achieve stable tet-controlled gene expression in cell populations. However, disadvantages of this strategy for integration into primary cells led us to develop an “All-In-One” vector system, enabling simultaneous integration of both components. The effect on tet-controlled gene expression was analyzed for retroviral “All-In-One” vectors expressing the M2-transactivator either under control of a constitutive or a new type of autoregulated promoter.

Results

Determination of luciferase activity in transduced cell populations indicated improvement of the dynamic range of gene expression for the autoregulated system. Further differences were observed regarding induction kinetics and dose–response. Most notably, introduction of the autoregulated system resulted in a threshold mode of induction, whereas the constitutive system exhibited pronounced effector-dose dependence.

Conclusion

Tet-regulated gene expression in the applied autoregulated system resembles a threshold mode, whereby full induction of the tet-unit can be achieved at otherwise limiting doxycycline concentrations.     

Indigenous and traditional plants: South African parents’ knowledge,perceptions and uses and their children’s sensory acceptance

Background

The dietary shift from indigenous and traditional plants (ITPs) to cash crops and exotic plant food sources increases the risk of malnutrition and other nutrition-related non-communicable diseases, especially in poor rural communities. Farm communities in South Africa have been associated with poor nutritional status and extreme poverty. ITPs have been found to be affordable sources of several micronutrients. However, knowledge of and the use of these plants are declining, and little is known about the child’s acceptance of dishes prepared with ITPs. This knowledge can be used to improve the general acceptance of ITPs. This study aimed to gain insight into parents’ knowledge and perceptions and their use of ITPs in a farming community in the North West Province and to assess children’s acceptance of and preference for dishes made with African leafy vegetables (ALVs) and Swiss chard.

Methods

Parents (n?=?29) responsible for food preparation for children in grade 2 to 4 in two schools were purposively selected for four focus group discussions. A sensory evaluation assessed the children’s (n?=?98) acceptance of, preference for and intended consumption of dishes made with leafy vegetables. The dishes were made of Amaranthus spp., Cleome gynandra, Cucurbita maxima, Vigna unguiculata and Beta vulgaris.

Results

Parents mentioned 30 edible ITPs during the focus group discussions. Parents had knowledge of available ITPs and their use as food. Location, seasonal variation and rainfall affected the availability of and access to ITPs. Sun-dried ITPs were stored in sacks for later use. ITPs were perceived as healthy, affordable and delicious, hence acceptable to the parents. The children also evaluated the dishes made with ALVs as acceptable in terms of colour, smell and taste. Swiss chard was preferred, most likely because of the children’s exposure to this vegetable. Children indicated that they would like to eat these leafy vegetables twice a week.

Conclusion

These results look promising for the promotion of ITPs as a strategy to reduce malnutrition in rural farm communities and for potential inclusion of these micronutrient-rich ALVs in school feeding programmes to improve the nutritional status of children.

     

Simple signaling games of sexual selection (Grafen’s revisited)

We investigate several versions of a simple game of sexual selection, to explore the role of secondary sexual characters (the “handicap paradox”) with the tools of signaling theory. Our models admit closed form solutions. They are very much inspired by Grafen’s (J Theor Biol 144:517–546, 1990a; J Theor Biol 144:473–516, 1990b) seminal companion papers. By merging and simplifying his two approaches, we identify a not so minor artifact in the seminal study. We propose an alternative model to start with Grafen’s sexual selection theory, with several similarities with Getty (Anim Behav 56:127–130, 1998).     

Symptomatic hypopituitarism revealing primary suprasellar lymphoma

Background

Cause-specific mortality is a commonly used endpoint of clinical trials or prospective studies. However, it is sometimes difficult for physician to determine the underlying-cause-of-death (UCD), especially for diabetic patients coexisted with cardiovascular diseases (CVD). The aim of this survey was to examine whether internists with different specialties have different opinions on the reporting of diabetes as the UCD.

Methods

A total of 549 physicians completed the questionnaire in Taiwan, which comprised seven hypothetical case scenarios, each indicating a different level of contribution of diabetes in initiating the chain of events leading to death.

Results

As a whole, endocrinologists were more likely than cardiologists and nephrologists to report diabetes as the UCD. The differences were more prominent when the diabetic patient had a coexisting CVD. In scenario 3 (a diabetic patient with hypertension who died from acute myocardial infarction), the percentage was 56% in endocrinologists, which was significantly higher than in cardiologists (42%) and nephrologists (41%). In scenario 4 (a diabetic patient with hypertension who died from cerebrovascular infarction), the percentage was 45% in endocrinologists, and only 31% in cardiologists and 36% in nephrologists.

Conclusions

Internists of different sub-specialties do have different opinions on the reporting of diabetes as the UCD, especially when the diabetic patient has a coexisting CVD.     

Adequate magnesium nutrition mitigates adverse effects of heat stress on maize and wheat

Aims

Heat stress is a growing concern in crop production because of global warming. In many cropping systems heat stress often occurs simultaneously with other environmental stress factors such as mineral nutrient deficiencies. This study aimed to investigate the role of adequate magnesium (Mg) nutrition in mitigating the detrimental effects of heat stress on wheat (Triticum aestivum) and maize (Zea mays).

Methods

Wheat and maize plants were grown in solution culture with low or adequate Mg supply at 25/22 °C (light/dark). Half of the plants were, then, exposed to heat stress at 35/28 °C (light/dark). Development of leaf chlorosis and changes in root and shoot growth, chlorophyll and Mg concentrations as well as the activities of major antioxidative enzymes were quantified in the experimental plants. Additionally, maize plants were analyzed for the specific weights (e.g., dry or fresh weight per a given leaf surface area) and soluble carbohydrate concentrations of sink and source leaves.

Results

Visual leaf symptoms of Mg deficiency were aggravated in wheat and maize when exposed to heat stress. In both species, root growth was more sensitive to Mg deficiency than shoot growth, and the shoot-to-root ratios peaked when heat stress was combined with Mg deficiency. Magnesium deficiency markedly reduced soluble carbohydrate concentrations in young leaf; but resulted in substantial increase in source leaves. Magnesium deficiency also increased activities of antioxidative enzymes, especially when combined with heat stress. The highest activities of superoxide dismutase (up to 80 % above the control), glutathione reductase (up to 250 % above the control) and ascorbate peroxidase (up to 300 % above the control) were measured when Mg-deficient plants were subjected to heat, indicating stimulated formation of reactive oxygen species (ROS) in Mg deficient leaves under heat stress.

Conclusions

Magnesium deficiency increases susceptibility of wheat and maize plants to heat stress, probably by increasing oxidative cellular damage caused by ROS. Ensuring a sufficiently high Mg supply for crop plants through Mg fertilization is a critical factor for minimizing heat-related losses in crop production.     

Genetic analysis of the effect of zinc deficiency on Arabidopsis growth and mineral concentrations

Aims

Zinc deficiency is a common micronutrient deficiency in plants growing in many different regions of the world and is associated with disturbances in uptake and accumulation of mineral nutrients. Despite many published data on physiological factors affecting ion accumulation in Zn deficient plants, there is very little information about the genetic factors underlying this. We aim to identify genetic loci involved in mineral accumulation and plant performance under Zn deficiency.

Methods

Genetic loci were identified using the genetically segregating Ler × Cvi recombinant inbred line (RIL) population grown under Zn deficient conditions. Lines were analysed for the concentrations of Zn, Fe, Mn, K, Ca, Mg, P, Cu, S and Al in shoot dry matter. The same was done for the same lines grown under Zn sufficient conditions.

Results

We found considerable heritable variation for most mineral concentrations. In general, there was a positive correlation between mineral concentrations. For Zn only condition-dependent QTLs were identified, while for most other mineral concentrations both condition-dependent and -independent QTLs were identified. Several QTLs co-localize, including co-localization to loci controlling shoot biomass and to mineral concentration loci found previously in this and other RIL populations.

Conclusions

There are different genetic loci controlling Zn accumulation under deficient and sufficient Zn supply. Only for few minerals, their accumulation is controlled by Zn-supply-specific loci.     

Evidence for progressive phosphorus limitation over long-term ecosystem development: Examination of a biogeochemical paradigm

Aims

To test predictions of ecosystem theory for changes in P cycling over primary succession, we determined soil phosphorus (P) in labile, primary mineral, organic, and occluded forms along a chronosequence of five wave cut terraces known as the “Ecological Staircase”. The Ecological Staircase terraces (T1-T5) transition naturally from fertile native coastal forests in California, USA, to diminutive pygmy vegetation over the span of?>?500,000 years of pedogenesis.

Methods

Soil P fractions were quantified to a depth of 40 cm on T1-T5 using a modified Hedley P fractionation procedure.

Results

Overall results confirmed the Walker and Syers Model of Phosphorus Transformations During Pedogenesis: total P declined from youngest (194 mg/kg P) to oldest (127 mg/kg P) sites; primary-mineral P decreased sharply from T1 to older sites; and occluded P dominated P pools at the oldest pygmy sites (T3-T5). In addition, foliar P concentrations declined markedly in the pygmy forest, and N/P of vegetation (T1: 6.03, T5: 14.4) and N/P_organic of mineral soils (T1: 6.10, T5: 25.3) increased significantly over time.

Conclusions

Results point to P as the primary limiting nutrient in the pygmy forest, exemplifying the terminal steady-state of ecosystem retrogression that underlies the persistence of this unique ecosystem.