四川美姑县大风顶国家级自然保护区小型兽类生物多样性分析

为评价美姑大风顶国家级自然保护区小型兽类多样性,利用香农一威纳多样性指数和一种基于信息测度的GF指数。经计算,香农一威纳多样性指数表明：农田一耕地为主的生境类型的小型兽类,以四川短尾鼩、高山姬鼠、黄胸鼠和社鼠为主要的群落组成,其物种多样性指数(H)和均匀性指数(J)分别是1．1374和0．8599;原始森林为主的生境类型的小型兽类,以川西白腹鼠和食虫类为主,其各多样性指数H=0．3307和J=0．3465;高山草甸为主的生境类型的小型兽类,以鼠兔类和姬鼠类为主,其H=0．3704和J=0．3881。为了得到补充和对照,参考了该区小型兽类已有的资料运用G-F指数评价其生物物种多样性,首先计算科间多样性(F)和属间多样性(G),然后,再利用F指数和G指数的比值进行标准化处理,得出GF指数值,说明保护区小型兽类种属间的多样性。该区小型兽类D-F指数为0．6977,即小型兽类种属间多样性较高,但和香农一威纳指数评定多样性有一定差异。保护区的建设还需进一步加强,包括人为干扰在内的一切干扰尚需排除。     

乌鲁木齐市不同生境繁殖期的鸟类群落多样性及功能集团分析

2016—2019年对乌鲁木齐市7种生境(高寒草甸、针叶林-灌丛、草原、农田、荒漠、湿地和城市公园)的繁殖期鸟类进行实地调查。共记录鸟类178种,隶属19目45科,其中雀形目Passeriformes最具代表性,共86种(占48.3%);居留型以夏候鸟和留鸟为主,共153种(占86.0%);分布型以古北型为主,共82种(占46.1%);珍稀濒危和重点保护物种共33种(占18.5%)。鸟类群落物种数及个体数最多的为湿地生境,荒漠、针叶林-灌丛生境次之,各生境物种数(H=30.628,P0.001)存在显著性差异。湿地生境鸟类的Shannon-Wiener多样性指数最高,高寒草甸生境的最低;草原生境的Pielou均匀度指数最高,高寒草甸生境的最低;高寒草甸生境的Simpson优势度指数最高,草原生境的最低;针叶林-灌丛生境的物种丰富度指数最高,荒漠生境的最低。草原与针叶林-灌丛生境之间的Sorensen相似性系数最高(0.59),高寒草甸与荒漠之间的最低(0)。湿地生境鸟类群落的捕食集团以食虫性为主,其他生境以杂食性为主。湿地生境中鸟类营地面巢的居多,高寒草甸生境中鸟类营洞巢的居多,其他生境中鸟类营编织巢的居多。上述结果表明,乌鲁木齐市周围的湿地生境应该得到优先保护。     

甘肃河西走廓不同生境中鼠类群落结构初步研究

本文对河西走廓不同生境中鼠类群落的物种组成和多样性进行了初步研究,在6种生境中进行了定量调查和采集,共获13种标本,隶于6（亚）科9属,其中古北界种类占优势。多样性分析结果表明：6种生境的物种丰富度指数RMargaler在.6139-1.9689之间。Shannon-Wiener指数（H')为1.0695-1.5607,Pielou旨数（J')和Simpson指数（D）分别为0.7242-0.9735和0．2768－0．4683,不同生境的丰富种数量（N1)变化趋势与H'相似,而非常丰富种数量（N2）的变化趋势为：半莫荒灌丛生境＞山地草原生境＞戈壁荒漠生境＞农田生境＞森林生境＞山地半荒漠灌丛生境。降水对不同生境类型中鼠类群落物种组成和多样性起直接的限制作用,同时海拔和人类干扰也是影响的因素。     

阿拉善地区不同生境小型兽类群落多样性研究

本文对内蒙古阿拉善地区不同生境中小型兽类群落的物种组成和多样性进行了初步研究。1998 年8～9 月和1999 年8～9 月, 2002 年10 月, 在8 种不同生境类型中共布放54 个样地, 22 606 个有效铗日, 捕获小型兽类标本866 只, 分属2 目6 科, 共20 种。多样性分析结果表明: 8 种不同生境的小型兽类群落Shannon-Wiener 多样性指数在1.186～1.589 之间; Pielou 均匀性指数在0.630 9～0.861 8 之间; Simpson 生态优势度指数在0.234 9～0.415 7 之间; Margalef 丰富度指数在0.931 8～2.020 之间。根据不同生境的小型兽类群落相似性指数聚类结果,可以将8 种生境的小型兽类群落大致划分为4 种类型: 耐旱型(群落Ⅴ和群落Ⅶ)、干旱型(群落Ⅰ、Ⅱ、Ⅲ)、农林交错型(群落Ⅵ和群落Ⅷ) 和森林型(群落Ⅳ) 。生境条件的差异对小型兽类群落物种组成和多样性有一定的限制作用, 而人为干扰有明显影响。     

东灵山地区啮齿动物群落组成及优势种群的季节变动

2000年9月和2001年9月在东灵山地区采用夹夜法对小型啮齿动物群落进行调查。共捕获啮齿动物6种,总捕获率为16．08％。大林姬鼠是该地区小型啮齿动物群落中的优势种。同时将8种生境样地划分为7类啮齿动物群落：即落叶松人工林大林姬鼠 社鼠群落、灌丛大林姬鼠 社鼠群落、辽东栎矮林大林姬鼠 大仓鼠群落、弃耕地大林姬鼠 黑线姬鼠群落、油松人工林大林姬鼠 社鼠群落、针润混交林大林姬鼠 社鼠群落、辽东栎林大林姬鼠 社鼠群落。群落间的相似性系数普遍较高,其中油松人工林大林姬鼠 社鼠群落和辽东栎林大林姬鼠 社鼠群落的相似性最高。灌丛大林姬鼠 社鼠群落的多样性指数和均匀性指数最高。     

甘肃河西走廊不同生境中鼠类群落结构初步研究

本文对河西走廊不同生境中鼠类群落的物种组成和多样性进行了初步研究,在6种生境中进行了定量调查和采集,共获13种标本,隶于6（亚）科9属,其中古北界种类占优势。多样性分析结果表明：6种生境的物种丰富度指数R_Margalef在0.6139～1.9689之间,Shannon-Wiener指数（H′）为1.0695～1.5607,Pielou指数（J′）和Simpson指数（D）分别为0.7242～0.9735和0.2768～0.4683,不同生境的丰富种数量（N₁）变化趋势与H′相似,而非常丰富种数量（N₂）的变化趋势为：半荒漠灌丛生境>山地草原生境>戈壁荒漠生境>农田生境>森林生境>山地半荒漠灌丛生境。降水对不同生境类型中鼠类群落物种组成和多样性起直接的限制作用,同时海拔和人类干扰也是影响的因素。     

延安人工林区小型兽类群落的季节动态

利用生物量、物种数、多样性指数、均匀度指数等变量, 对延安人工林区小型兽类群落的季节性变化进行了调查。选择生物量作为小型兽类的优势度指标, 分析了小型兽类优势度的季节性变化及在不同生境的表现。共捕获小型兽类9 种, 分属2 目3 科, 优势种为达乌尔鼠兔和中华鼢鼠甘肃亚种; 小型兽类群落4 种变量的季节变化与小型兽类种群生物量的季节变化关系密切; 延安人工林区小型兽类群落优势种的季节性决定着群落生物量的季节性, 普通种和稀有种的季节性决定着物种数和多样性的季节性; 延安人工林区9 种小型兽类存在不同的时空组合; 中华鼢鼠是唯一在各季节和所有生境中分布的种类; 夏、秋季刺槐人工林区、杂草灌丛区达乌尔鼠兔优势度极高。     

三峡重庆库区不同鸟类群落的物种组成及多样性研究

1996～1997年,对三峡工程重庆库区的鸟类群落物种组成和种群数量进行了调查,共调查记录库区鸟类15目48科226种,数量18929只。用鸟类数量级划分、Sorensen相似性指数、Shannon-Wiener指数和Pielou指数分析库区各种生境鸟类群落物组成和物种多样性结果表明：1）库区天然林、人工林、灌草丛和水域生境中鸟类群落的组成与农田生境具有较高的相似性,尤以人工林和农田的相似性最高;2）库区人工林生境鸟类群落物种多样性最高;3）库区由于长期以来人类活动的影响,生境日趋单一,已逐渐形成一种以农田灌丛鸟类为主的鸟类生态系统。     

寒温带牧林交错区生境复杂度对啮齿类物种多样性的影响

采用铗日法对嫩江流域牧林交错区5个生境梯度中啮齿动物的多样性水平进行了研究,以探讨栖息地复杂程度对地面啮齿类物种多样性的影响.结果表明,黑线姬鼠、黑线仓鼠和大林姬鼠是嫩江流域牧林交错区中的优势种和常见种,不同物种在不同生境类型中呈明显的不均匀分布,反映了它们对不同生境的选择倾向性.同时,地面草本植物的覆盖度和丰富度对地面活动的小型哺乳动物的物种组成和群落结构具有较大的影响,啮齿动物的捕获率和生物量与草本植物的多样性水平呈现出显著的相关性关系,其物种丰富度随草本植物多样性指数的增加呈递增的趋势.此外,各生境中啮齿动物的物种多样性指数与草本植物多样性的相关关系并不显著,可能是因为灌丛区和林-灌交错区内大量的低矮灌丛与萌生丛改善了生境的隐蔽条件与食物资源,为更多物种的共存创造了良好的微环境,使得两生境中啮齿动物的物种多样性水平尤为突出.研究表明,小型啮齿动物的物种组成与栖息地的复杂程度有关,但地面植被对小型兽类生物量和物种多样性的影响力度并不是等同的,对地面小型兽类物种多样性影响的研究,统计生境复杂度或异质性时应考虑不同植被型/生境类型距地面的高度,不同植被型对小型兽类物种多样性的贡献程度随其距地面高度的增加而降低.     

小兴安岭林区不同生境梯度中小型哺乳动物生物多样性

利用铗日法和陷阱法,于2000年7～8月,对小兴安岭林区7个不同生境梯度中小型哺乳动物群落的多样性变化进行了初步研究。结果表明：(1)铗日法所捕获的物种数和个体数明显大于陷阱法．但后者更适合捕获小体型动物;(2)共捕获小型哺乳动物9种．其中啮齿目(Rodentia)松鼠科(Sciuridae)1种．田鼠亚科(Microtine)3种．鼠科(Muridae)4种．食虫目(Insectivora)鼩鲭科(Soricidae)1种．所捕获的物种中．棕背解(Clethrionomys rufocanus)为优势种．占总数的48．77％．与黑线姬鼠(Apodemus agrarius )、大林姬鼠(A．speciosus)和红背Ping(C．rutilus)一起构成本地区的常见种;(3)物种在不同的生境类型中呈明显的不均匀分布．以中度干扰的灌丛生境内小型哺乳动物从物种丰富度和多样性指数最高．次生林1和农田生境均有较高的物种丰富度和多样性指数,无干扰的原生林1、2中物种丰富度最低,而轻微干扰的原生林3中物种多样性指数最低．受严重干扰的农田生境中小型哺乳动物均匀度最高．其次是次生林1和灌丛,原生林3和干扰较重的次生林2中小型哺乳动物群落均匀度最小;(4)从物种多样性与林地郁闭度和地表草本植物覆盖度的相关分析看出：物种多样性与郁闭度相关性不显著．而与覆盖度呈显著的正相关关系．反映了这些物种对草本植物选择倾向;(5)根据不同生境内群落相似性指数进行系统聚类,将所有的生境聚类为3组．即原生林组(原生林1、原生林2和原生林3)、次生林组(次生林1和次生林2)和农田组(灌丛和农田)．表明组内各生境间小型哺乳动物成分的相似性和组间的明显差异。研究表明．小型哺乳动物多样性受生境内植被演替阶段、食物和隐蔽条件等环境因素影响．而人类干扰也是主要因素之一。     

Role for dopamine in malonate-induced damage in vivo in striatum and in vitro in mesencephalic cultures

Defects in mitochondrial energy metabolism have been implicated in the pathology of several neurodegenerative disorders. In addition, the reactive metabolites generated from the metabolism and oxidation of the neurotransmitter dopamine (DA) are thought to contribute to the damage to neurons of the basal ganglia. We have previously demonstrated that infusions of the metabolic inhibitor malonate into the striata of mice or rats produce degeneration of DA nerve terminals. In the present studies, we demonstrate that an intrastriatal infusion of malonate induces a substantial increase in DA efflux in awake, behaving mice as measured by in vivo microdialysis. Furthermore, pretreatment of mice with tetrabenazine (TBZ) or the TBZ analogue Ro 4-1284 (Ro-4), compounds that reversibly inhibit the vesicular storage of DA, attenuates the malonate-induced DA efflux as well as the damage to DA nerve terminals. Consistent with these findings, the damage to both DA and GABA neurons in mesencephalic cultures by malonate exposure was attenuated by pretreatment with TBZ or Ro-4. Treatment with these compounds did not affect the formation of free radicals or the inhibition of oxidative phosphorylation resulting from malonate exposure alone. Our data suggest that DA plays an important role in the neurotoxicity produced by malonate. These findings provide direct evidence that inhibition of succinate dehydrogenase causes an increase in extracellular DA levels and indicate that bioenergetic defects may contribute to the pathogenesis of chronic neurodegenerative diseases through a mechanism involving DA.     

2018年中国植物科学若干领域重要研究进展

2018年中国植物科学继续呈现快速发展的态势, 我国科学家在国际植物科学主流学术刊物发表论文数量大幅增加, 取得了多项具有重要影响的成果。调控植物生长-代谢平衡实现可持续农业发展入选2018年度中国科学十大进展; 中国被子植物区系进化历史研究入选2018年度中国生命科学十大进展。以水稻为代表的农作物和果蔬等经济作物研究在国际上已呈现出明显的优势, 若干领域已从“追赶”状态跨越到“领跑”地位。该文对2018年中国科学家在植物科学若干领域取得的重要研究成果进行了概括性评述, 旨在全面追踪和报道当前中国植物科学领域的发展前沿和热点, 展示中国科学家所取得的杰出成就。     

我国葫芦科植物离体培养研究进展

葫芦科植物包括多种瓜类蔬菜,对其进行离体培养研究具有重要的理论和实践意义。综述了国内在葫芦科植物器官培养、体细胞胚胎发生、花药培养、原生质体培养和体细胞杂交及离体遗传转化等方面取得的研究进展,并对葫芦科植物离体培养、遗传转化与育种的前景作了展望。     

SSTR5 ablation in islet results in alterations in glucose homeostasis in mice

Somatostatin (SST) peptide is a potent inhibitor of insulin secretion and its effect is mediated via somatostatin receptor 5 (SSTR5) in the endocrine pancreas. To investigate the consequences of gene ablation of SSTR5 in the mouse pancreas, we have generated a mouse model in which the SSTR5 gene was specifically knocked down in the pancreatic beta cells (betaSSTR5Kd) using the Cre-lox system. Immunohistochemistry analysis showed that SSTR5 gene expression was absent in beta cells at three months of age. At the time of gene ablation, betaSSTR5Kd mice demonstrated glucose intolerance with lack of insulin response and significantly reduced serum insulin levels. Insulin tolerance test demonstrated a significant increase of insulin clearance in vivo at the same age. In vitro studies demonstrated an absence of response to SST-28 stimulation in the betaSSTR5Kd mouse islet, which was associated with a significantly reduced SST expression level in betaSSTR5Kd mice pancreata. In addition, betaSSTR5Kd mice had significantly reduced serum glucose levels and increased serum insulin levels at 12 months of age. Glucose tolerance test at an older age also indicated a persistently higher insulin level in betaSSTR5Kd mice. Further studies of betaSSTR5Kd mice had revealed elevated serum C-peptide levels at both 3 and 12 months of age, suggesting that these mice are capable of producing and releasing insulin to the periphery. These results support the hypothesis that SSTR5 plays a pivotal role in the regulation of insulin secretion in the mouse pancreas.     

2017年中国植物科学若干领域重要研究进展

2017年中国植物科学继续保持高速发展态势, 重大成果频出, 具体表现在中国植物学家在国际顶级学术期刊发表的文章数量平稳上升。中国植物科学领域的研究工作者成果精彩纷呈, 如新型广谱抗病机制的发现、水稻广谱抗病遗传基础及机制和疫霉菌诱发病害成灾机制研究等。2017年中国生命科学领域十大进展评选中, 有两项植物科学领域的研究成果入选。水稻生物学、进化与基因组学和激素生物学等领域学科发展突出。另外, 值得一提的是, 长期从事高等植物与代谢途径调控分子网络研究和水稻品种设计育种的李家洋院士的研究成果“水稻高产优质性状形成的分子机理及品种设计”荣获2017年国家自然科学一等奖。这一具有重大国际影响的开创性贡献标志着中国植物科学在该领域的国际科学前沿居于引领和卓越地位。该文对2017年中国本土科学家在植物科学若干领域取得的重要研究成果进行了系统梳理, 旨在全面追踪和报道当前中国植物科学领域发展的最新前沿动态, 与广大读者共同分享我国科学家所取得的辉煌成就。     

2018年中国植物科学若干领域重要研究进展

2018年中国植物科学继续呈现快速发展的态势, 我国科学家在国际植物科学主流学术刊物发表论文数量大幅增加, 取得了多项具有重要影响的成果。调控植物生长-代谢平衡实现可持续农业发展入选2018年度中国科学十大进展; 中国被子植物区系进化历史研究入选2018年度中国生命科学十大进展。以水稻为代表的农作物和果蔬等经济作物研究在国际上已呈现出明显的优势, 若干领域已从“追赶”状态跨越到“领跑”地位。该文对2018年中国科学家在植物科学若干领域取得的重要研究成果进行了概括性评述, 旨在全面追踪和报道当前中国植物科学领域的发展前沿和热点, 展示中国科学家所取得的杰出成就。     

我国葫芦科植物离体培养研究进展

葫芦科植物包括多种瓜类蔬菜,对其进行离体培养研究具有重要的理论和实践意义.综述了国内在葫芦科植物器官培养、体细胞胚胎发生、花药培养、原生质体培养和体细胞杂交及离体遗传转化等方面取得的研究进展,并对葫芦科植物离体培养、遗传转化与育种的前景作了展望.     

Changes in aquatic vegetation in Rhine floodplain streams in Alsace in relation to disturbance

Abstract. Changes are described in aquatic vegetation in oligotrophic, groundwater-fed Rhine floodplain streams in Alsace (eastern France), resulting from disturbance. Disturbance factors include changes in nutrients, either permanent ones - effluent from a waste water treatment plant or trout hatcheries - or periodic ones: flooding. Regular inputs of high levels of phosphate and ammonia modified the macrophyte vegetation in these streams. The floristic composition, which was characteristic of oligotrophic waters upstream of the eutrophicated sector, changed to that of a eutrophic situation as originally found downstream. Periodic disturbance by floods which normally occur once a year, irregularly eutrophicates the small streams, causing the development of a mixture of eutrophic and oligotrophic species. Six macrophyte communities are distinguished, indicating different trophic levels. The aquatic vegetation is adapted to the variations of phosphate and ammonia levels. Hence, aquatic macrophytes can be used as bio-indicators of fluctuations in water nutrient levels in relation to the type of disturbance.     

Apoptosis in experimental myocardial infarction in situ and in the perfused heart in vitro

We report the appearance of apoptotic cells in experimental myocardial infarction (rabbit heart) in in situ and in vitro preparations. Apoptosis was recognized by intravital staining with Hoechst 33342 (Ho342), by nick-end labeling (TUNEL) and by DNA laddering. A steady rise in the relative number of apoptotic cardiomyocytes (apoptotic index) was noted in in situ preparations. Apoptosis was first noted 6 h after the onset of ischemia with its highest value occurring after 72 h. Apoptotic nuclei were absent in remote areas of the left and right ventricles. Apoptotic nuclei within the infarcted area showed diminished intensity of Ho342 fluorescence. Three days after ischemia, a border zone adjacent to the infarcted area consisting of apoptotic macrophages was recognized. A novel finding was the appearance of apoptotic cardiomyocytes in the isolated perfused ischemic heart. Occurring as early as 50 min after the onset of ischemia, a high apoptotic index was present adjacent to the ligature placed around the coronary artery. This observation provides the opportunity to selectively examine factors leading to apoptosis in the ischemic heart under controlled experimental conditions.     

Morphine-potentiated platelet aggregation in in vitro and platelet plug formation in in vivo experiments

The detailed mechanisms underlying morphine-signaling pathways in platelets remain obscure. Therefore, we systematically examined the influence of morphine on washed human platelets. In this study, washed human platelet suspensions were used for in vitro studies. Furthermore, platelet thrombus formation induced by irradiation of mesenteric venules with filtered light in mice pretreated with fluorescein sodium was used for an in vivo thrombotic study. Morphine concentration dependently (0.6, 1, and 5 microM) potentiated platelet aggregation and the ATP release reaction stimulated by agonists (i.e., collagen and U46619) in washed human platelets. Yohimbine (0.1 microM), a specific alpha(2)-adrenoceptor antagonist, markedly abolished the potentiation of morphine in platelet aggregation stimulated by agonists. Morphine also potentiated phosphoinositide breakdown and intracellular Ca(2+) mobilization in human platelets stimulated by collagen (1 microg/ml). Moreover, morphine (0.6-5 microM) markedly inhibited prostaglandin E(1) (10 microM)-induced cyclic AMP formation in human platelets, while yohimbine (0.1 microM) significantly reversed the inhibition of cyclic AMP by morphine (0.6 and 1 microM) in this study. The thrombin-evoked increase in pH(i) was markedly potentiated in the presence of morphine (1 and 5 microM). Morphine (2 and 5 mg/g) significantly shortened the time require to induce platelet plug formation in mesenteric venules. We concluded that morphine may exert its potentiation in platelet aggregation by binding to alpha(2)-adrenoceptors in human platelets, with a resulting inhibition of adenylate cyclase, thereby reducing intracellular cyclic AMP formation followed by increased activation of phospholipase C and the Na(+)/H(+) exchanger. This leads to increased intracellular Ca(2+) mobilization, and finally potentiation of platelet aggregation and of the ATP release reaction.