共查询到20条相似文献,搜索用时 15 毫秒
1.
N-banded karyotypes of wheat species 总被引:2,自引:0,他引:2
W. L. Gerlach 《Chromosoma》1977,62(1):49-56
Nine of the twenty-one chromosome pairs of the hexaploid wheat Triticum aestivum var. Chinese Spring (genome constitution AABBDD) show distinctive N-banding patterns. These nine chromosomes are 4A, 7A and all of the B genome chromosomes. The remaining chromosomes show either faint bands or no bands at all. Tetraploid wheat, T. dicoccoides (AABB), showed banded chromosomes similar to those observed in the hexaploid. Of the diploid species T. monococcum, T. boeoticum, T. urartu and Aegilops sauarrosa showed little or no banding as would be expected of donors of the A and D genomes. Ae. speltoides had a number of N-banded chromosomes as would be expected of a candidate for the B genome donor. Since N-bands are not evident on some nucleolar organiser chromosomes, the staining specificity cannot be correlated with the presence of nucleolar organiser regions. 相似文献
2.
Two pairs of chromosomes (1U and 5U) in Aegilops umbellulata possess ribosomal RNA genes. This has been proven by studying wheat plants into which 1U and 5U chromosomes have been introduced separately. These plants have more ribosomal RNA genes than the recipient wheat plants and additional clusters of rDNA when examined by in situ hybridisation. The repeating rDNA unit in Aegilops umbellulata is longer than most of the units in the wheat variety Chinese Spring, the additional DNA probably being in the non-transcribed spacer. This was determined from restriction endonuclease maps of rDNA. In Chinese Spring plants possessing 1U or 5U chromosomes, the largest nucleoli are formed on 1U or 5U chromosomes and the wheat nucleolus organisers form micronucleoli. This is not because the nucleolus organisers on chromosomes 1U and 5U have many more rRNA genes than wheat nucleolus organisers. It is suggested that the Aegilops umbellulata nucleolus organisers are dominant over those of wheat because they compete more effectively for some limiting factor. — The partial inactivation of the wheat nucleolus organisers by chromosomes 1U or 5U does not result in a reduced total nucleolus volume in root tip or pollen mother cells, because of the compensation by the nucleolus organisers of chromosomes 1U or 5U. The amount of RNA in seedlings is not markedly affected by the partial inactivation of the wheat nucleolus organisers. 相似文献
3.
Schoenenberger N Felber F Savova-Bianchi D Guadagnuolo R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(7):1338-1346
Introgression from allohexaploid wheat (Triticum aestivum L., AABBDD) to allotetraploid jointed goatgrass (Aegilops cylindrica Host, CCDD) can take place in areas where the two species grow in sympatry and hybridize. Wheat and Ae. cylindrica share the D genome, issued from the common diploid ancestor Aegilops tauschii Coss. It has been proposed that the A and B genome of bread wheat are secure places to insert transgenes to avoid their introgression
into Ae. cylindrica because during meiosis in pentaploid hybrids, A and B genome chromosomes form univalents and tend to be eliminated whereas
recombination takes place only in D genome chromosomes. Wheat random amplified polymorphic DNA (RAPD) fragments, detected
in intergeneric hybrids and introgressed to the first backcross generation with Ae. cylindrica as the recurrent parent and having a euploid Ae. cylindrica chromosome number or one supernumerary chromosome, were assigned to wheat chromosomes using Chinese Spring nulli-tetrasomic
wheat lines. Introgressed fragments were not limited to the D genome of wheat, but specific fragments of A and B genomes were
also present in the BC1. Their presence indicates that DNA from any of the wheat genomes can introgress into Ae. cylindrica. Successfully located RAPD fragments were then converted into highly specific and easy-to-use sequence characterised amplified
regions (SCARs) through sequencing and primer design. Subsequently these markers were used to characterise introgression of
wheat DNA into a BC1S1 family. Implications for risk assessment of genetically modified wheat are discussed. 相似文献
4.
Marker-assisted pyramiding of two cereal cyst nematode resistance genes from <Emphasis Type="Italic">Aegilops variabilis</Emphasis> in wheat 总被引:1,自引:0,他引:1
Dominique Barloy Jocelyne Lemoine Paulette Abelard A. M. Tanguy Roger Rivoal Joseph Jahier 《Molecular breeding : new strategies in plant improvement》2007,20(1):31-40
The cereal cyst nematode (CCN) Heterodera avenae, is a significant pathogen of wheat. The wild grass Aegilops variabilis Accession No.1 has been found to be resistant to pathotypes of CCN; at least two genes transferred to wheat, designated as
CreX and CreY, are involved in the resistance response. The CreY gene may be the same as Rkn-mn1, which confers resistance to root knot nematode (RKN) Meloidogyne naasi. The objective of this work was to pyramid the two CCN resistance genes in a wheat background through marker-assisted selection.
As a first step, molecular markers flanking CreX were identified. The completely linked RAPD marker of Rkn-mn1 (CreY), OpY16-1065, previously obtained, was converted into a SCAR. All these dominant markers were used to incorporate in the same genotype the
two Ae. variabilis chromosome segments carrying the two genes for resistance. CCN bioassays with the Ha12 pathotype showed that the level of
resistance of the pyramided line was significantly higher than that of CreX and CreY single introgression lines, but lower than that of Ae. variabilis. This study thus illustrates the utilization of molecular markers in breeding for host resistance. 相似文献
5.
J. R. Lacadena M. C. Cermeño 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,71(2):278-283
Summary The nucleolar organizer activity of wheat (Triticum aestivum, AABBDD) and Aegilops umbellulata (UU) chromosomes have been analyzed in the complete set of the chromosome addition lines by using a highly reproducible silver-staining procedure. Chromosomes 1U and 5U produce the partial inactivation of wheat nucleolar organizer chromosomes 6B, 1B and 5D. The chromosomes D and G from Ae. umbellulata, which are not SAT-chromosomes, seem to specifically influence the activity of wheat NORs. The predominant status of the U genome with respect to nucleolar competition in the Triticeae is confirmed. 相似文献
6.
The application of wheat microsatellites to identify disomic Triticum aestivum-Aegilops markgrafii addition lines 总被引:7,自引:0,他引:7
A. Peil V. Korzun V. Schubert E. Schumann W. E. Weber M. S. Röder 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):138-146
We describe the use of wheat microsatellites for the discrimination of Aegilops markgrafii chromosomes. Twenty out of eighty eight wheat microsatellites (WMS) tested were able to distinguish Triticum aestivum-Ae. markgrafii addition lines. Six, three, three, one and six of 18 WMS can be used as markers for single Ae. markgrafii chromosomes B, C, D, F and G, respectively. Addition line A is not available but additional bands, appearing only in Ae. markgrafii and the T. aestivum-Ae. markgrafii amphiploid and not in any of the available addition lines, indicate that three WMS detect markers for Ae. markgrafii chromosomes A. Addition line E could not be detected by any of the WMS markers applied, although the 20 WMS represented all
the homologous groups of wheat. All three WMS located on the short arm of group-2 chromosomes were located on Ae. markgrafii chromosome B; three of four WMS, located on the long arm of wheat group-2 chromosomes, were specific to Ae. markgrafii chromosome G and three of four WMS, specific to group-5 chromosomes, were markers for Ae. markgrafii chromosome C, indicating the homoeology of these wheat chromosome arms with the respective Ae. markgrafii chromosomes.
Received: 29 May 1997 / Accepted: 10 September 1997 相似文献
7.
C. Parisod C. Definod A. Sarr N. Arrigo F. Felber 《Journal of evolutionary biology》2013,26(1):223-228
Introgression of sequences from crop species in wild relatives is of fundamental and practical concern. Here, we address gene flow between cultivated wheat and its widespread polyploid relative, Aegilops triuncialis, using 12 EST‐SSR markers mapped on wheat chromosomes. The presence of wheat diagnostic alleles in natural populations of the barbed goatgrass growing in proximity to cultivated fields highlights that substantial gene flow occurred when both species coexisted. Furthermore, loci from the A subgenome of wheat were significantly less introgressed than sequences from other subgenomes, indicating differential introgression into Ae. triuncialis. Gene flow between such species sharing nonhomeologous chromosomes addresses the evolutionary outcomes of hybridization and may be important for efficient gene containment. 相似文献
8.
B. Fernández-Calvín J. Orellana 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,83(2):264-272
Summary Meiotic associations of different wheat-Aegilops variabilis and wheat-Ae. kotschyi hybrid combinations with low and high homoeologous pairing were analyzed at metaphase I. Five types of pairing involving wheat and Aegilops genomes were identified by using C-banding. A genotype that seems to promote homoeologous pairing has been found in Ae. variabilis var. cylindrostachys. Its effect is detectable in the low pairing hybrids but not in the high ones. Pairing affinity has been analyzed on the basis of metaphase I associations in the low and high homoeologous pairing hybrids, and in bivalents and multivalents in the high pairing hybrids. The results indicate that the amount of bound arms of each type of identifiable association relative to the total associations formed (relative contribution) was not maintained, either between the different levels of pairing (low and high) or between different meiotic configurations (bivalents and multivalents). These findings seem to indicate that quantifications of genomic relationships based on the amount of chromosome pairing at metaphase I must be carefully done in this type of hybrid combinations. 相似文献
9.
Control of Endosperm Proteins in TRITICUM AESTIVUM (Var. Chinese Spring) and AEGILOPS UMBELLULATA by Homoeologous Group 1 Chromosomes 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The genetic control of major wheat endosperm proteins by homoeologous group 1 chromosomes has been studied by two-dimensional polyacrylamide gel electrophoresis. The control of at least 15 distinct protein subunits or groups of protein subunits has been allocated to chromosomes 1A, 1B and 1D of Chinese Spring wheat from the analysis of grains of aneuploid genotypes. In addition, six protein subunits have been shown to be controlled by chromosome 1Cu of the related species, Aegilops umbellulata, from studies of wheat lines carrying disomic substitutions of 1Cu chromosomes. On the basis of protein subunit patterns, chromosome 1Cu is more closely related to chromosome 1D of wheat than to chromosomes 1A or 1B. 相似文献
10.
This study was planned to identify the chromosomal location of esterase loci in wheat (Triticum aestivum), in comparison to Aegilops uniaristata, using wheat Ae. uniaristata disomic addition and translocation lines. Two loci (Est-N1 and Est-N8) were identified on 3N chromosome of Ae. uniaristata and their probable homoeoloci were, for the first time, mapped close to three RFLP probes (Xpsr56, Xpsr394, and Xpsr1196) on homoeologous group 3 wheat chromosomes. 相似文献
11.
The influence of individual Aegilops longissima addition chromosomes on callus induction and organogenesis of in vitro cultivated immature embryos of wheat was studied. Immature 14-day-old embryos of seven Chinese Spring lines with added chromosomes of Aegilops longissima were in vitro cultivated. Although relatively high number of calli were formed in embryo cultures of all lines studied, the different genotypes expressed variation in respect to their response to in vitro cultivation. The number of initiated calli of line D, containing chromosome l l arms 7S L and 4S L was relatively low. The lines showed substantial differences in respect to the number of initiated morphogenic calli and regenerants recovered. The highest number of morphogenic calli and regenerants l l l were obtained in cultures of lines containing the chromosomes 2S , 6S and to less extend 3S. 相似文献
12.
Haili Zhang Qiulan Huang Ling Yi Xiaona Song Lin Li Guangbing Deng Junjun Liang Fang Chen Maoqun Yu Hai Long 《The Plant journal : for cell and molecular biology》2021,107(3):698-712
The pathogen cereal cyst nematode (CCN) is deleterious to Triticeae crops and is a threat to the global crop yield. Accession no. 1 of Aegilops variabilis, a relative of Triticum aestivum (bread wheat), is highly resistant to CCN. Our previous study demonstrated that the expression of the phenylalanine ammonia lyase (PAL) gene AevPAL1 in Ae. variabilis is strongly induced by CCN. PAL, the first enzyme of phenylpropanoid metabolism, is involved in abiotic and biotic stress responses. However, its role in plant–CCN interaction remains unknown. In the present study, we proved that AevPAL1 helps to confer CCN resistance through affecting the synthesis of salicylic acid (SA) and downstream secondary metabolites. The silencing of AevPAL1 increased the incidence of CCN infection in roots and decreased the accumulation of SA and phenylalanine (Phe)-derived specialized metabolites. The exogenous pre-application of SA also improved CCN resistance. Additionally, the functions of PAL in phenylpropanoid metabolism correlated with tryptophan decarboxylase (TDC) functioning in tryptophan metabolism pathways. The silencing of either AevPAL1 or AevTDC1 exhibited a concomitant reduction in the expression of both genes and the contents of metabolites downstream of PAL and TDC. These results suggested that AevPAL1, possibly in coordination with AevTDC1, positively contributes to CCN resistance by altering the downstream secondary metabolites and SA content in Ae. variabilis. Moreover, AevPAL1 overexpression significantly enhanced CCN resistance in bread wheat and did not exhibit significant negative effects on yield-related traits, suggesting that AevPAL1 is valuable for the genetic improvement of CCN resistance in bread wheat. 相似文献
13.
H. Zhang J. Jia M. D. Gale K. M. Devos 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):69-75
A comparative genetic map of Aegilops umbellulata with wheat was constructed using RFLP probes that detect homoeoloci previously mapped in hexaploid bread wheat. All seven
Ae. umbellulata chromosomes display one or more rearrangements relative to wheat. These structural changes are consistent with the sub-terminal
morphology of chromosomes 2 U, 3 U, 6 U and 7 U. Comparison of the chromosomal locations assigned by mapping and those obtained
by hybridization to wheat/Ae. umbellulata single chromosome addition lines verified the composition of the added Ae. umbellulata chromosomes and indicated that no further cytological rearrangements had taken place during the production of the alien-wheat
aneuploid lines. Relationships between Ae. umbellulata and wheat chromosomes were confirmed, based on homoeology of the centromeric regions, for 1 U, 2 U, 3 U, 5 U and 7 U. However,
homoeology of the centromeric regions of 4 U with wheat group-6 chromosomes and of 6 U with wheat group-4 chromosomes was
also confirmed, suggesting that a re-naming of these chromosomes may be pertinent. The consequences of the rearrangements
of the Ae. umbellulata genome relative to wheat for gene introgression are discussed.
Received: 10 July 1997 / Accepted: 19 September 1997 相似文献
14.
An efficient screening for terminal deletions and translocations of barley chromosomes added to common wheat 总被引:6,自引:0,他引:6
Ingo Schubert Fang Shi Jörg Fuchs Takashi R. Endo 《The Plant journal : for cell and molecular biology》1998,14(4):489-495
As a prerequisite to determine physical gene distances in barley chromosomes by deletion mapping, a reliable, fast and inexpensive approach was developed to detect terminal deletions and translocations in individual barley chromosomes added to the chromosome complement of common wheat. A refined fluorescence in situ hybridization (FISH) technique subsequent to N-banding made it possible to detect subtelomeric repeat sequences (HvT01) on all 14 chromosome arms of barley. Some chromosome arms could be distinguished individually based on the number of FISH signals or the intensity of terminal FISH signals. This allowed the detection and selection of deletions and translocations of barley chromosomes (exemplified by 7H and 4HL), which occurred in the progeny of the wheat lines containing a pair of individual barley chromosomes (or telosomes) and a single so-called gametocidal chromosome (2C) of Aegilops cylindrica. This chromosome is known to cause chromosomal breakage in the gametes in which it is absent. Terminal deletions and translocations in barley chromosomes were easily recognized in metaphase and even in interphase nuclei by a decrease in the number of FISH signals specific to the subtelomeric repeat. These aberrations were verified by genomic in situ hybridization. The same approach can be applied to select deletions and translocations of other barley chromosomes in wheat lines that are monosomic for the Ae. cylindrica chromosome 2C. 相似文献
15.
The relationships of three wheat-Aegilops longissima chromosome addition lines A, C, and D with homoeologous wheat chromosomes were studied in PMC meiosis. Substitutions of alien chromosome A for wheat chromosome 6 B, chromosome C for 1 B and chromosome D for 4 B were obtained. The production of 4 BS/C and 7 BS/D chromosome translocations indicated cytogenetic relationships of C partially to homoeologous wheat chromosomes of group 1 and 4, and D partially to homoeologous wheat chromosomes of group 4 and 7. 相似文献
16.
Chromosome markers in the tetraploid wheat Aegilops ventricosa analysed by in situ hybridization 总被引:1,自引:0,他引:1
D. Bardsley A. Cuadrado P. Jack G. Harrison A. Castilho J. S. Heslop-Harrison 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):300-304
Three lines of the tetraploid wheat Aegilops ventricosa Tausch (2n=4x=28), which contains good resistance to eyespot, were analysed using fluorescent in situ hybridization. Probes used included
rDNA, cloned repeated sequences from wheat and rye, simple-sequence repeats (SSRs) and total genomic DNA. The banding patterns
produced could be used to distinguish most chromosome arms and will aid in the identification of Ae. ventricosa chromosomes or chromosome segments in breeding programmes. All lines had a single major 18S-25S rDNA site, the nucleolar
organizing region (NOR) in chromosome 5N and several minor sites of 18S-25S rDNA and 5S rDNA. A 1NL.3DL, 1NS.3DS translocation
was identified, and other minor differences were found between the lines.
Received: 11 August 1998 / Accepted: 28 November 1998 相似文献
17.
The Giemsa C-banding pattern of the chromosomes of the native self-pollinatedAegilops comosa subsp.comosa var.comosa was studied. Six of the seven chromosomes of the haploid genome were found to be polymorphic for C-banding patterns. Chromosome A had four variants, chromosome E three variants and each of the chromosomes B, D, and F two variants. Chromosomes E and G were polymorphic for arm length and arm ratio.This paper is part of the doctoral dissertation ofA. Georgiou. 相似文献
18.
Dr. V. Jaaska 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1980,56(6):273-284
Summary Evolutionary and ontogenetic variation of six seedling esterases of independent genetic control is studied in polyploid wheats and their diploid relatives by means of polyacrylamide gel electrophoresis. Four of them are shown to be controlled by homoeoallelic genes in chromosomes of third, sixth and seventh homoeologous groups.The isoesterase electrophoretic data are considered supporting a monophyletic origin of both the primitive tetraploid and the primitive hexaploid wheat from which contemporary taxa of polyploid wheats have emerged polyphyletically and polytopically through recurrent introgressive hybridization and accumulation of mutations. Ancestral diploids belonging or closely related to Triticum boeoticum, T. urartu, Aegilops speltoides and Ae. tauschii ssp. strangulata are genetically the most suitable genome donors of polyploid wheats. Diploids of the Emarginata subsection of the section Sitopsis, Aegilops longissima s.str., Ae. sharonensis, Ae. searsii and Ae. bicornis, are unsuitable for the role of the wheat B genome donors, being all fixed for the esterase B and D electromorphs different from those of tetraploid wheats. 相似文献
19.
Marta Cifuentes Elena Benavente 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(8):1609-1616
The pattern of homoeologous metaphase I (MI) pairing has been fully characterized in durum wheat × Aegilops cylindrica hybrids (2n = 4x = 28, ABCcDc) by an in situ hybridization procedure that has permitted individual discrimination of every wheat and wild constituent genome.
One of the three hybrid genotypes examined carried the ph1c mutation. In all cases, MI associations between chromosomes of both species represented around two-third of total. Main results
from the analysis are as follows (a) the A genome chromosomes are involved in wheat–wild MI pairing more frequently than the
B genome partners, irrespective of the alien genome considered; (b) both durum wheat genomes pair preferentially with the
Dc genome of jointed goatgrass. These findings are discussed in relation to the potential of genetic transference between wheat
crops and this weedy relative. It can also be highlighted that inactivation of Ph1 provoked a relatively higher promotion of MI associations involving B genome. 相似文献
20.
Shattering is an essential seed dispersal mechanism in wild species. It is believed that independent mutations at orthologous loci led to convergent domestication of cereal crops. To investigate genetic relationships of Triticeae shattering genes with those of other grasses, we mapped spike-, barrel- (B-type), and wedge-type (W-type) spikelet disarticulation genes in wheat and its wild relatives. The Br1 gene for W-type disarticulation was mapped to a region delimited by Xpsr598 and Xpsr1196 on the short arm of chromosomes 3A in Triticum timopheevii and 3S in Aegilops speltoides. The spike- and W-type disarticulation genes are allelic at Br1 in Ae. speltoides. The B-type disarticulation gene, designated as Br2, was mapped to an interval of 4.4 cM between Xmwg2013 and Xpsr170 on the long arm of chromosome 3D in Aegilops tauschii, the D-genome donor of common wheat. Therefore, B- and W-type disarticulations are governed by two different orthologous loci on group-3 chromosomes. Based on map position, orthologs of Br1 and Br2 were not detected in barley, maize, rice, and sorghum, indicating multiple genetic pathways for shattering in grasses. The implications of the mapping results are discussed with regard to the evolution of polyploid wheat and domestication of cereals.Supplementary material is available in the online version of this article at 相似文献