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1.
The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five unique isolates not belonging to a particular group were identified. Most groups were restricted to one geographical area, indicating a limited distribution of related cyanobacterial strains. An extensive cyanobacterial diversity was found both within and between the 11 different Gunnera species. Within a particular plant and even within the same stem gland, more than one cyanobacterial strain at a time could be present. These results indicate a low specificity in Gunnera-Nostoc symbiosis. 相似文献
2.
Genetic diversity and classification of cyanobacteria in different Azolla species by the use of PCR fingerprinting 总被引:6,自引:0,他引:6
W. W. Zheng M. Nilsson B. Bergman U. Rasmussen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(7-8):1187-1193
Symbiotically associated cyanobacteria from 18 accessions within all known species in the genus Azolla were examined and classified by the use of polymerase chain reaction (PCR)-fingerprinting. A repetitive sequence specific
for cyanobacteria, the short tandemly repeated repetitive (STRR) sequence, was used as a primer in the reaction. Cyanobacterial
filaments isolated directly from the Azolla leaf cavity or contained within homogenised symbiotic Azolla tissue were used as templates. Based on the fingerprint pattern, distinct differences were demonstrated between cyanobacteria
isolated from the Euazolla and Rhizosperma sections. In addition, individual fingerprints were obtained from all cyanobacteria isolated from the different Azolla species. The fingerprints were used to generate a phylogenetic tree. Three clusters were distinguished: one contained the
four isolates from the section Euazolla, a second the isolate from Azolla filiculoides, and a third the three isolates from the section Rhizosperma. By the use of STRR-PCR fingerprinting, new data on the taxonomy of cyanobacteria in Azolla were obtained, which have been difficult to generate by other classification methods. PCR-fingerprinting may, therefore,
be a valuable tool for diversity and classification studies of symbiotic cyanobateria from Azolla and, as co-evolution between the cyanobacteria and its corresponding host exists the method may also be useful for the taxonomy
of Azolla.
Received: 19 December 1998 / Accepted: 31 May 1999 相似文献
3.
The presence of repetitive DNA sequences viz., short tandemly repeated repetitive (STRR) and highly iterated palindrome (HIP), in the cyanobacterial genome were used to
generate a PCR-based fingerprint pattern of nine cyanobacterial cultures (both stress tolerant and non-tolerant), belonging
to the genus Westiellopsis. By this method it was possible to generate distinguishing fingerprint patterns for all the isolates and cluster isolates
with similar stress tolerance properties. This study reveals the utility of repetitive DNA sequences in the cyanobacterial
genome, for differentiation of Westiellopsis cultures and clustering strains that posses similar stress tolerance properties. 相似文献
4.
Akoijam Chingkheihunba Singh Arvind Kumar 《World journal of microbiology & biotechnology》2011,27(9):2187-2194
The diversity of cyanobacteria in the North-Eastern region of India has not been studied except for a few sporadic and inconclusive
reports. Loktak Lake is a huge reservoir for various kinds of organisms, including cyanobacteria. The present study describes
the isolation and molecular diversity of 72 filamentous, heterocystous cyanobacterial strains isolated from samples collected
from Loktak Lake, its adjoining rice fields and rice fields in Indian Council of Agricultural Research (ICAR) complex, Shillong,
Meghalaya, India. The isolated strains belonged to the genera Anabaena, Nostoc, Calothrix, Cylindrospermum and Mastigocladus. The molecular analysis of isolates revealed the occurrence of certain strains being present in the sample collected from
the rice fields falling in the catchment area of Loktak Lake, Manipur and rice fields in ICAR complex, Shillong, Meghalaya
both. A polyphasic approach based on morphological features and PCR based molecular polymorphism revealed enormous level of
molecular diversity. Out of three primers targeted regions used for determining genetic polymorphism, STRR1A produced best
fingerprint profile of cyanobacterial strains. The morphological diversity of isolates was assured by light microscope whereas
PCR based multiple fingerprint profile was used for molecular characterization. Molecular typing using short tandemly repeated
repetitive STRR1A sequences as primer provided strain specific fingerprint profiles of the isolates. 相似文献
5.
Molecular Characterization of the Toxic Cyanobacterium Cylindrospermopsis raciborskii and Design of a Species-Specific PCR 总被引:1,自引:0,他引:1
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Kim M. Wilson Mark A. Schembri Peter D. Baker Christopher P. Saint 《Applied microbiology》2000,66(1):332-338
Cylindrospermopsis raciborskii is a toxic-bloom-forming cyanobacterium that is commonly found in tropical to subtropical climatic regions worldwide, but it is also recognized as a common component of cyanobacterial communities in temperate climates. Genetic profiles of C. raciborskii were examined in 19 cultured isolates originating from geographically diverse regions of Australia and represented by two distinct morphotypes. A 609-bp region of rpoC1, a DNA-dependent RNA polymerase gene, was amplified by PCR from these isolates with cyanobacterium-specific primers. Sequence analysis revealed that all isolates belonged to the same species, including morphotypes with straight or coiled trichomes. Additional rpoC1 gene sequences obtained for a range of cyanobacteria highlighted clustering of C. raciborskii with other heterocyst-producing cyanobacteria (orders Nostocales and Stigonematales). In contrast, randomly amplified polymorphic DNA and short tandemly repeated repetitive sequence profiles revealed a greater level of genetic heterogeneity among C. raciborskii isolates than did rpoC1 gene analysis, and unique band profiles were also found among each of the cyanobacterial genera examined. A PCR test targeting a region of the rpoC1 gene unique to C. raciborskii was developed for the specific identification of C. raciborskii from both purified genomic DNA and environmental samples. The PCR was evaluated with a number of cyanobacterial isolates, but a PCR-positive result was only achieved with C. raciborskii. This method provides an accurate alternative to traditional morphological identification of C. raciborskii. 相似文献
6.
The cyanobacteria belonging to the genus Nostoc fix atmospheric nitrogen, both as free-living organisms and in symbiotic associations
with a wide range of hosts, including bryophytes, gymnosperms (cycads), the small water fern Azolla (Pteridophyte), the angiosperm
genus Gunnera, and fungi (lichens). The Gunnera–Nostoc symbiosis is the only one that involves a flowering plant. In Chile,
12 species of Gunnera have been described with a broad distribution in the temperate region. We examined the genetic diversity
of Nostoc symbionts from three populations of Gunnera tinctoria from Abtao, Chiloé Island, southern Chile, and microsymbionts
from other two species of Gunnera from southern Chile, using PCR amplification of STRR (short tandemly repeated repetitive)
sequences of the Nostoc infected tissue. To our knowledge, this is the first report of PCR fingerprinting obtained directly
from symbiotic tissue of Gunnera. Genetic analyses revealed that Nostoc symbionts exhibit important genetic diversity among
host plants, both within and between Gunnera populations. It was also found that only one Nostoc strain, or closely related
strains, established symbiosis with an individual plant host. 相似文献
7.
A phylogenetic analysis of selected symbiotic Nostoc strain sequences and available database 16S rDNA sequences of both symbiotic and free-living cyanobacteria was carried out using maximum likelihood and Bayesian inference techniques. Most of the symbiotic strains fell into well separated clades. One clade consisted of a mixture of symbiotic and free-living isolates. This clade includes Nostoc sp. strain PCC 73102, the reference strain proposed for Nostoc punctiforme. A separate symbiotic clade with isolates exclusively from Gunnera species was also obtained, suggesting that not all symbiotic Nostoc species can be assigned to N. punctiforme. Moreover, isolates from Azolla filiculoides and one from Gunnera dentata were well nested within a clade comprising most of the Anabaena sequences. This result supports the affiliation of the Azolla isolates with the genus Anabaena and shows that strains within this genus can form symbioses with additional hosts. Furthermore, these symbiotic strains produced hormogonia, thereby verifying that hormogonia formation is not absent in Anabaena and cannot be used as a criterion to distinguish it from Nostoc.The GenBank accession numbers for the cyanobacterial 16S rRNA gene sequences determined in this study are AY742447-AY742454. 相似文献
8.
The presence of repeated DNA, viz. short tandemly repeated repetitive (STRR) and highly iterated palindrome (HIP) sequences was used as a typing technique for assessing genetic variability and phylogenetic relatedness of heterocystous cyanobacteria. Primers analogous to the STRR and HIP sequences were used to generate specific fingerprints for the twelve heterocystous cyanobacterial strains and a dendrogram was constructed. STRRmod and HIPTG primers revealed 100% polymorphism and yielded almost identical patterns. Anabaena sp. PCC 7120 clustered with Nostoc muscorum with both primers. Primer STRRmod supported the heterogeneity between Nostoc and Anabaena but HIPTG placed these two genera distinctly apart. STRRmod and HIPTG revealed that the members of the two orders were intermixed and thus suggesting a monophyletic origin of heterocystous cyanobacteria. 相似文献
9.
Fengjiao Zhang Fadi Chen Trude Schwarzacher J S Heslop-Harrison Nianjun Teng 《Annals of botany》2023,131(1):215
Background and AimsTandemly repeated DNA and transposable elements represent most of the DNA in higher plant genomes. High-throughput sequencing allows a survey of the DNA in a genome, but whole-genome assembly can miss a substantial fraction of highly repeated sequence motifs. Chrysanthemum nankingense (2n = 2x = 18; genome size = 3.07 Gb; Asteraceae), a diploid reference for the many auto- and allopolyploids in the genus, was considered as an ancestral species and serves as an ornamental plant and high-value food. We aimed to characterize the major repetitive DNA motifs, understand their structure and identify key features that are shaped by genome and sequence evolution.MethodsGraph-based clustering with RepeatExplorer was used to identify and classify repetitive motifs in 2.14 millions of 250-bp paired-end Illumina reads from total genomic DNA of C. nankingense. Independently, the frequency of all canonical motifs k-bases long was counted in the raw read data and abundant k-mers (16, 21, 32, 64 and 128) were extracted and assembled to generate longer contigs for repetitive motif identification. For comparison, long terminal repeat retrotransposons were checked in the published C. nankingense reference genome. Fluorescent in situ hybridization was performed to show the chromosomal distribution of the main types of repetitive motifs.Key ResultsApart from rDNA (0.86 % of the total genome), a few microsatellites (0.16 %), and telomeric sequences, no highly abundant tandem repeats were identified. There were many transposable elements: 40 % of the genome had sequences with recognizable domains related to transposable elements. Long terminal repeat retrotransposons showed widespread distribution over chromosomes, although different sequence families had characteristic features such as abundance at or exclusion from centromeric or subtelomeric regions. Another group of very abundant repetitive motifs, including those most identified as low-complexity sequences (9.07 %) in the genome, showed no similarity to known sequence motifs or tandemly repeated elements.ConclusionsThe Chrysanthemum genome has an unusual structure with a very low proportion of tandemly repeated sequences (~1.02 %) in the genome, and a high proportion of low-complexity sequences, most likely degenerated remains of transposable elements. Identifying the presence, nature and genomic organization of major genome fractions enables inference of the evolutionary history of sequences, including degeneration and loss, critical to understanding biodiversity and diversification processes in the genomes of diploid and polyploid Chrysanthemum, Asteraceae and plants more widely. 相似文献
10.
Genetic Characterization of Escherichia coli Populations from Host Sources of Fecal Pollution by Using DNA Fingerprinting 总被引:3,自引:1,他引:2
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Escherichia coli isolates were obtained from common host sources of fecal pollution and characterized by using repetitive extragenic palindromic (REP) PCR fingerprinting. The genetic relationship of strains within each host group was assessed as was the relationship of strains among different host groups. Multiple isolates from a single host animal (gull, human, or dog) were found to be identical; however, in some of the animals, additional strains occurred at a lower frequency. REP PCR fingerprint patterns of isolates from sewage (n = 180), gulls (n = 133), and dairy cattle (n = 121) were diverse; within a host group, pairwise comparison similarity indices ranged from 98% to as low as 15%. A composite dendrogram of E. coli fingerprint patterns did not cluster the isolates into distinct host groups but rather produced numerous subclusters (approximately >80% similarity scores calculated with the cosine coefficient) that were nearly exclusive for a host group. Approximately 65% of the isolates analyzed were arranged into host-specific groups. Comparable results were obtained by using enterobacterial repetitive intergenic consensus PCR and pulsed-field gel electrophoresis (PFGE), where PFGE gave a higher differentiation of closely related strains than both PCR techniques. These results demonstrate that environmental studies with genetic comparisons to detect sources of E. coli contamination will require extensive isolation of strains to encompass E. coli strain diversity found in host sources of contamination. These findings will assist in the development of approaches to determine sources of fecal pollution, an effort important for protecting water resources and public health. 相似文献
11.
12.
Molecular characterization of the toxic cyanobacterium Cylindrospermopsis raciborskii and design of a species-specific PCR 总被引:1,自引:0,他引:1
Cylindrospermopsis raciborskii is a toxic-bloom-forming cyanobacterium that is commonly found in tropical to subtropical climatic regions worldwide, but it is also recognized as a common component of cyanobacterial communities in temperate climates. Genetic profiles of C. raciborskii were examined in 19 cultured isolates originating from geographically diverse regions of Australia and represented by two distinct morphotypes. A 609-bp region of rpoC1, a DNA-dependent RNA polymerase gene, was amplified by PCR from these isolates with cyanobacterium-specific primers. Sequence analysis revealed that all isolates belonged to the same species, including morphotypes with straight or coiled trichomes. Additional rpoC1 gene sequences obtained for a range of cyanobacteria highlighted clustering of C. raciborskii with other heterocyst-producing cyanobacteria (orders Nostocales and Stigonematales). In contrast, randomly amplified polymorphic DNA and short tandemly repeated repetitive sequence profiles revealed a greater level of genetic heterogeneity among C. raciborskii isolates than did rpoC1 gene analysis, and unique band profiles were also found among each of the cyanobacterial genera examined. A PCR test targeting a region of the rpoC1 gene unique to C. raciborskii was developed for the specific identification of C. raciborskii from both purified genomic DNA and environmental samples. The PCR was evaluated with a number of cyanobacterial isolates, but a PCR-positive result was only achieved with C. raciborskii. This method provides an accurate alternative to traditional morphological identification of C. raciborskii. 相似文献
13.
PCR amplification techniques viz., repetitive DNA element PCR (REP-PCR), short tandemly repeated repetitive PCR (STRR-PCR)
and arbitrarily primed PCR (RAPD-PCR) were used for the taxonomic discrimination among the strains of the unicellular cyanobacterium
Synechococcus elongatus collected across the coastal regions of the Indian subcontinent. These strains showed similar phenotypic and genotypic characteristics.
Data obtained from genomic fingerprinting were used to perform cluster analysis and demonstrated ability to differentiate
strains at intra-specific level. Polymorphisms of different PCR amplification products can serve as strain-specific molecular
fingerprints. In comparison with the STRR and RAPD, the REP primer set generates fingerprints of lower complexity, but still
the phenogram clearly differentiated the strains. In conclusion, described PCR fingerprinting methods can be considered as
promising tools for the differentiation at the strain level of cyanobacteria from the same species. 相似文献
14.
Cyanobacteria separated from symbiosis with several species of the angiospermGunnera were comparatively characterized and correlated with the locales and taxonomy of their host plants. All were identified as strains ofNostoc. Protein profiles and DNA restriction fragment length polymorphisms (from hybridizations with heterologousnifH andglnA probes) determined that three of the four cyanobacteria fromGunnera grown at one site in Sweden, each from a different host species, were very similar or identical. Plants of one species,G. manicata, grown in a second location at the site were infected with a different cyanobiont. Among five isolates from two species ofGunnera, collected in the same locale in New Zealand, three subgroups were documented. Isolates from three differentGunnera species grown in separate locations in the United States were each uniquely different. None of the cyanobacteria differed in the molecular weights of their glutamine synthetase and Fe-nitrogenase proteins. The diversity and accessibility of compatibleNostoc populations present in the soil micro-environment, not a critical selective factor required byGunnera, were concluded to be a major determinant in symbiont selection. 相似文献
15.
Matthew A. Parker 《Applied microbiology》1999,65(11):4914-4920
Multilocus enzyme electrophoresis, partial 23S rRNA sequences, and nearly full-length 16S rRNA sequences all indicated high genetic similarity among root-nodule bacteria associated with Apios americana, Desmodium glutinosum, and Amphicarpaea bracteata, three common herbaceous legumes whose native geographic ranges in eastern North America overlap extensively. A total of 19 distinct multilocus genotypes (electrophoretic types [ETs]) were found among the 35 A. americana and 33 D. glutinosum isolates analyzed. Twelve of these ETs (representing 78% of all isolates) were either identical to ETs previously observed in A. bracteata populations, or differed at only one locus. Within both 23S and 16S rRNA genes, several isolates from A. americana and D. glutinosum were either identical to A. bracteata isolates or showed only single nucleotide differences. Growth rates and nitrogenase activities of A. bracteata plants inoculated with isolates from D. glutinosum were equivalent to levels found with native A. bracteata bacterial isolates, but none of the three A. americana isolates tested had high symbiotic effectiveness on A. bracteata. Phylogenetic analysis of both 23S and 16S rRNA sequences indicated that both A. americana and D. glutinosum harbored rare bacterial genotypes similar to Bradyrhizobium japonicum USDA 110. However, the predominant root nodule bacteria on both legumes were closely related to Bradyrhizobium elkanii. 相似文献
16.
The RNase P RNA from cyanobacteria: short tandemly repeated repetitive (STRR) sequences are present within the RNase P RNA gene in heterocyst-forming cyanobacteria. 总被引:10,自引:1,他引:9
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A Vioque 《Nucleic acids research》1997,25(17):3471-3477
The RNase P RNA gene (rnpB) from 10 cyanobacteria has been characterized. These new RNAs, together with the previously available ones, provide a comprehensive data set of RNase P RNA from diverse cyanobacterial lineages. All heterocystous cyanobacteria, but none of the non-heterocystous strains analyzed, contain short tandemly repeated repetitive (STRR) sequences that increase the length of helix P12. Site-directed mutagenesis experiments indicate that the STRR sequences are not required for catalytic activity in vitro. STRR sequences seem to have recently and independently invaded the RNase P RNA genes in heterocyst-forming cyanobacteria because closely related strains contain unrelated STRR sequences. Most cyanobacteria RNase P RNAs lack the sequence GGU in the loop connecting helices P15 and P16 that has been established to interact with the 3'-end CCA in precursor tRNA substrates in other bacteria. This character is shared with plastid RNase P RNA. Helix P6 is longer than usual in most cyanobacteria as well as in plastid RNase P RNA. 相似文献
17.
A fingerprinting technique similar to repetitive extragenic palindromic PCR was developed to identify strains of Lactococcus lactis. The method distinguishes closely related strains and discriminates among some with identical ldh sequences. The fingerprinting primer LL-Rep1 complements a moderately repeated sequence found in low G+C Gram-positive bacteria and may therefore prove useful for discriminating among strains of other low G+C Gram-positive species. 相似文献
18.
Genetic and Biochemical Diversity among Isolates of Paenibacillus alvei Cultured from Australian Honeybee (Apis mellifera) Colonies
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Steven P. Djordjevic Wendy A. Forbes Lisa A. Smith Michael A. Hornitzky 《Applied microbiology》2000,66(3):1098-1106
Twenty-five unique CfoI-generated whole-cell DNA profiles were identified in a study of 30 Paenibacillus alvei isolates cultured from honey and diseased larvae collected from honeybee (Apis mellifera) colonies in geographically diverse areas in Australia. The fingerprint patterns were highly variable and readily discernible from one another, which highlighted the potential of this method for tracing the movement of isolates in epidemiological studies. 16S rRNA gene fragments (length, 1,416 bp) for all 30 isolates were enzymatically amplified by PCR and subjected to restriction analysis with DraI, HinfI, CfoI, AluI, FokI, and RsaI. With each enzyme the restriction profiles of the 16S rRNA genes from all 30 isolates were identical (one restriction fragment length polymorphism [RFLP] was observed in the HinfI profile of the 16S rRNA gene from isolate 17), which confirmed that the isolates belonged to the same species. The restriction profiles generated by using DraI, FokI, and HinfI differentiated P. alvei from the phylogenetically closely related species Paenibacillus macerans and Paenibacillus macquariensis. Alveolysin gene fragments (length, 1,555 bp) were enzymatically amplified from some of the P. alvei isolates (19 of 30 isolates), and RFLP were detected by using the enzymes CfoI, Sau3AI, and RsaI. Extrachromosomal DNA ranging in size from 1 to 10 kb was detected in 17 of 30 (57%) P. alvei whole-cell DNA profiles. Extensive biochemical heterogeneity was observed among the 28 P. alvei isolates examined with the API 50CHB system. All of these isolates were catalase, oxidase, and Voges-Proskauer positive and nitrate negative, and all produced acid when glycerol, esculin, and maltose were added. The isolates produced variable results for 16 of the 49 biochemical tests; negative reactions were recorded in the remaining 30 assays. The genetic and biochemical heterogeneity in P. alvei isolates may be a reflection of adaptation to the special habitats in which they originated. 相似文献
19.
Hima J. Khamar Erick K. Breathwaite Christine E. Prasse Elizabeth R. Fraley Craig R. Secor Fairouz L. Chibane Jeff Elhai Wan-Ling Chiu 《Plant physiology》2010,154(3):1381-1389
Gunnera plants have the unique ability to form endosymbioses with N2-fixing cyanobacteria, primarily Nostoc. Cyanobacteria enter Gunnera through transiently active mucilage-secreting glands on stems. We took advantage of the nitrogen (N)-limitation-induced gland development in Gunnera manicata to identify factors that may enable plant tissue to attract and maintain cyanobacteria colonies. Cortical cells in stems of N-stressed Gunnera plants were found to accumulate a copious amount of starch, while starch in the neighboring mature glands was nearly undetectable. Instead, mature glands accumulated millimolar concentrations of glucose (Glc) and fructose (Fru). Successful colonization by Nostoc drastically reduced sugar accumulation in the surrounding tissue. Consistent with the abundance of Glc and Fru in the gland prior to Nostoc colonization, genes encoding key enzymes for sucrose and starch hydrolysis (e.g. cell wall invertase, α-amylase, and starch phosphorylase) were expressed at higher levels in stem segments with glands than those without. In contrast, soluble sugars were barely detectable in mucilage freshly secreted from glands. Different sugars affected Nostoc’s ability to differentiate motile hormogonia in a manner consistent with their locations. Galactose and arabinose, the predominant constituents of polysaccharides in the mucilage, had little or no inhibitory effect on hormogonia differentiation. On the other hand, soluble sugars that accumulated in gland tissue, namely sucrose, Glc, and Fru, inhibited hormogonia differentiation and enhanced vegetative growth. Results from this study suggest that, in an N-limited environment, mature Gunnera stem glands may employ different soluble sugars to attract Nostoc and, once the cyanobacteria are internalized, to maintain them in the N2-fixing vegetative state.Nitrogen (N) is an essential element for plant growth, but availability of reduced N in the soil is often limiting. Representatives from a wide range of land plants have evolved the ability to form associations with N2-fixing microbes (Franche et al., 2009). Associations between rhizobia and legume plants are well-characterized examples of plant-bacterial N2-fixing symbioses. Unlike rhizobia, which generally exhibit narrow host ranges (Kistner and Parniske, 2002), N2-fixing cyanobacteria are able to form productive associations with a broad range of plants, including bryophytes (hornworts and liverworts), ferns (Azolla), gymnosperms (cycads), and angiosperms (Gunnera; for review, see Rai et al., 2000; Adams et al., 2006). Free-living cyanobacteria within the genus Nostoc can fix N in specialized microoxic cells called heterocysts. The ability of Nostoc to fix N independent of a host environment may facilitate the formation of symbioses with a wide range of plants. Understanding the physiological conditions that enable a plant host to enter into symbiotic associations with cyanobacteria may allow us to extend the benefit of biological N fixation to crops outside the legume family.Nostoc has the ability to differentiate not only into filaments bearing heterocysts but also into transiently motile filaments, known as hormogonia, which enable the cyanobacteria to enter plants (Meeks and Elhai, 2002). Nostoc can be induced to form hormogonia by different environmental stimuli and by a hormogonia-inducing factor released from N-stressed host plants (Meeks and Elhai, 2002; Adams et al., 2006). The attraction of hormogonia to plants is much less specific than that of rhizobia. Hormogonia are attracted to root extracts from either host or nonhost plants and even to certain simple sugars, such as Ara, Glc, and Gal (Nilsson et al., 2006). After entering a plant host, hormogonia revert back to filaments with N2-fixing heterocysts. Inside the host, further hormogonia formation is suppressed, and heterocysts appear at a frequency of about 30% to 40%, 3- to 4-times higher than that found in free-living Nostoc (Meeks and Elhai, 2002). Although free-living Nostoc species can support N2 fixation through photosynthesis, under symbiotic conditions they rely on photosynthate from the host plant. In general, the sugars (Suc, Glc, and Fru) known to support heterotrophic growth in the dark by free-living cyanobacteria coincide with those that support nitrogenase activity in Nostoc-plant associations (Meeks and Elhai, 2002). However, the Nostoc-Gunnera association may be exceptional; only Glc and Fru have been shown to sustain nitrogenase activities (Man and Silvester, 1994; Wouters et al., 2000), although Suc anddextrin were able to keep Nostoc alive without light (Wouters et al., 2000). It is evident from cyanobacterial studies that the plant hosts have evolved the ability to regulate cyanobacterial growth and differentiation during symbiotic associations (Meeks and Elhai, 2002).However, because most studies of plant-cyanobacterial associations have focused on the cyanobacterial partner (e.g. Wang et al., 2004; Ekman et al., 2006), the mechanisms through which plant hosts attract, internalize, and maintain cyanobacteria remain to be elucidated (Adams et al., 2006).The Nostoc-Gunnera association is an ideal system with which to study plant-cyanobacteria symbioses, not only because Gunnera is the only genus of angiosperms known to form endosymbioses with N2-fixing cyanobacteria but also because the association between the two can be readily established in the laboratory (Bergman et al., 1992; Chiu et al., 2005). Nostoc hormogonia enter Gunnera plants through specialized glands located on the stem. As the gland matures, it secretes polysaccharide-rich mucilage that attracts cyanobacteria (Nilsson et al., 2006), supports their growth on the gland surface (Towata, 1985; Chiu et al., 2005), and permits further hormogonia differentiation (Rasmussen et al., 1994). From there, hormogonia enter the gland and penetrate cells near the base of the gland in the stem (Bonnett, 1990; Bergman et al., 1992). Although each gland is only transiently capable of accepting cyanobacteria, new glands continue to form on the stem at the base of each new leaf.In contrast to the development of nodules in legumes, which requires a complex exchange of signals between the two symbiotic partners (Cooper, 2007), stem gland development in Gunnera takes place in the absence of cyanobacteria (Bonnett, 1990). N limitation, however, is a prerequisite for stem gland development (Chiu et al., 2005), as it is for nodulation (Barbulova et al., 2007). We have taken advantage of the N-deficiency-induced gland development in G. manicata to identify factors that enable Gunnera to form endosymbiosis with cyanobacteria. This study investigated changes in the carbohydrate metabolism during Gunnera gland development and discovered that tissue in the mature glands accumulated high levels of soluble sugars prior to the arrival of cyanobacteria. In agreement with this finding, several key genes encoding enzymes for starch/Suc hydrolysis were expressed at higher levels in the gland compared to the stem. Furthermore, we found that various sugars cyanobacteria may encounter as they approach Gunnera glands as opposed to those they would encounter within plant cells differentially affected Nostoc’s ability to form motile hormogonia. 相似文献
20.
Pseudomonas putida KT2440 is a soil bacterium that effectively colonises the roots of many plants and degrades a variety of toxic aromatic compounds. Its genome has recently been sequenced. We describe that a 35 bp sequence with the structure of an imperfect palindrome, originally found repeated three times downstream of the rpoH gene terminator, is detected more than 800 times in the chromosome of this strain. The structure of this DNA segment is analogous to that of the so-called enterobacteriaceae repetitive extragenic palindromic (REP) sequences, although its sequence is different. Computer-assisted analysis of the presence and distribution of this repeated sequence in the P.putida chromosome revealed that in at least 80% of the cases the sequence is extragenic, and in 82% of the cases the distance of this extragenic element to the end of one of the neighbouring genes was <100 bp. This 35 bp element can be found either as a single element, as pairs of elements, or sometimes forming clusters of up to five elements in which they alternate orientation. PCR scanning of chromosomes from different isolates of Pseudomonas sp. strains using oligonucleotides complementary to the most conserved region of this sequence shows that it is only present in isolates of the species P.putida. For this reason we suggest that the P.putida 35 bp element is a distinctive REP sequence in P.putida. This is the first time that REP sequences have been described and characterised in a group of non-enterobacteriaceae. 相似文献