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Ohne ZusammenfassungMit 2 Textfiguren  相似文献   

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Ohne ZusammenfassungMit 2 Textfiguren  相似文献   

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Ohne ZusammenfassungHierzu Tafel 6–8 und 2 Textfiguren.  相似文献   

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Ohne ZusammenfassungMit Tafel I u. II und 23 Fig. im Text.  相似文献   

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Silkworm strains resistant to Bombyx mori L. nuclear polyhedrosis virus were obtained through transgenic experiments. piggyBac transposon with an A3 promoter were randomly inserted into the silkworm, driving the enhanced green fluorescent protein (EGFP) reporter gene into the silkworm genome. Polymerase chain reaction results verified the insertion of the extraneous EGFP gene, and fluorescence microscopy showed that the EGFP was expressed in the midgut tissue. The morbidity ratio of the nuclear polyhedrosis decreased from 90% in the original silkworm strain to 66.7% in the transgenic silkworm strain. Compared with the resistance to the Bombyx mori L. nuclear polyhedrosis virus in the Qiufeng strain, which is commonly used in the production, there was an increase of 33 centesimal points in the transgenic silkworms. The antivirotic character in the Chunhua x Qiuyue strain, which was bred from a different transgenic family, was about 10 centesimal points higher than that in the Qiufeng x Baiyu, another crossbreed used in production. Our results indicated a good application value of the transposon-inserted mutation in the breeding of anti-BmNPV silkworm strain.  相似文献   

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Johannes Lehmann 《Planta》1973,114(1):51-61
Summary Tests for enzymes of gluconeogenesis and of the synthesis and degradation of sucrose and polysaccharides have been carried out in the phloem exudate of Cucurbita pepo. All the enzymes which are necessary for the synthesis of sucrose and polysaccharides from metabolites of the citric acid cycle were found to be present in the exudate, except phosphoenolpyruvate carboxykinase. The polysaccharide synthetase was found to exhibit higher activity with glycogen (which is an unnatural polysaccharide in higher plants) than with starch. In addition, polysaccharide synthetase activity could be increased remarkably with 2 mM glucose-6-phosphate and glycogen as primer. Among the enzymes which catabolize sucrose and polysaccharides (phosphorylase, invertase, sucrose phosphorylase), only sucrose phosphorylase showed activity.  相似文献   

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化性是家蚕的一种重要性状,不仅受遗传基因控制,还受温度、光线等外界环境条件的影响而发生变化。介绍了家蚕化性变化的因素及其机理,并根据家蚕化性的特性,论述了养蚕生产上调控家蚕化性以提高丝量丝质、防止生种与再生卵发生的技术措施。  相似文献   

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《Insect Biochemistry》1984,14(6):725-728
Prostaglandin synthetase has been isolated and purified from the post mitochondrial supernatant of the testis of male silkmoth Bombyx mori L. A 3-fold purification was achieved compared to that of post mitochondrial supernatant. The partially purified enzyme converted arachidonic acid to PGE2in vitro in the presence of haemin and hydroquinone.  相似文献   

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