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1.
广西酸性土地区季节性雨林的分类研究 总被引:2,自引:1,他引:1
季节性雨林是广西南部北热带地区的地带性植被类型。本文主要介绍酸性土地区季节性雨林的类型, 记录了下列几个群系:1、以窄叶坡垒为主的季节性雨林;2、以擎天树为主的季节性雨林;3、以小叶红光树为主的季节性雨林;4、以风吹楠为主的季节性雨林;5、以咛为主的季节性雨林;6、以紫荆木为主的季节性雨林;7、以橄榄为主的季节性雨林;8、以米老排为主的季节性雨林;9、以大果马蹄荷为主的季节性雨林;10、以红苞木为主的季节性雨林;11、以仪花为主的季节性雨林;12、以格木为主的季节性雨林;13、以翅子树为主的季节性雨林;14、以红鳞蒲桃为主的季节性雨林;并把已确定的群丛列举出来。 相似文献
2.
季节性雨林是广西南部北热带地区的地带性植被类型。本文主要介绍酸性土地区季节性雨林的类型, 记录了下列几个群系:1、以窄叶坡垒为主的季节性雨林;2、以擎天树为主的季节性雨林;3、以小叶红光树为主的季节性雨林;4、以风吹楠为主的季节性雨林;5、以嚜咛为主的季节性雨林;6、以紫荆木为主的季节性雨林;7、以橄榄为主的季节性雨林;8、以米老排为主的季节性雨林;9、以大果马蹄荷为主的季节性雨林;10、以红苞木为主的季节性雨林;11、以仪花为主的季节性雨林;12、以格木为主的季节性雨林;13、以翅子树为主的季节性雨林;14、以红鳞蒲桃为主的季节性雨林;并把已确定的群丛列举出来。 相似文献
3.
4.
广西石灰岩季节性雨林分类的研究 总被引:6,自引:3,他引:6
广西北热带地区石灰岩山地占据相当大的面积,特别是桂西南一带,那里形成一种独特的石灰岩季节性雨林,与相邻的砂页岩或花岗岩山地季节性雨林镶嵌分布。本文主要论述它的分类系统和地理分布规律。 相似文献
5.
We examined by using 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) as a radical generator the ability of estrogens to scavenge carbon-centered and peroxyl radicals. Electron spin resonance signals of carbon-centered radicals from AAPH were diminished by catecholestrogens but not by phenolic estrogens, showing that catecholestrogens efficiently scavenged carbon-centered radicals. However, fluorescent decomposition of R-phycoerythrin by AAPH-derived peroxyl radicals was inhibited by catecholestrogens and phenolic estrogens. Evidently, peroxyl radicals were scavenged by catecholestrogens and by phenolic estrogens. However, the scavenging ability of 4-hydroxyestradiol was less than 2-hydroxyestradiol. Strand break of DNA induced by AAPH was inhibited by catecholestrogens, but not by phenolic estrogens under aerobic and anaerobic conditions. Inactivation of lysozyme induced by AAPH was completely blocked by 2-hydroxyestradiol under aerobic and anaerobic conditions, and by 4-hyroxyestradiol only under anaerobic conditions. Peroxidation of arachidonic acid by AAPH was strongly inhibited by catecholestrogens at low concentrations. Only large amounts of phenolic estrogens markedly inhibited lipid peroxidation. These results show that catecholestrogens were antioxidant against AAPH-induced damage to biological molecules through scavenging both carbon-centered and peroxyl radicals, but phenolic estrogens partially inhibited AAPH-induced damage because they scavenged only peroxyl radicals. 相似文献
6.
Resistin release by human adipose tissue explants in primary culture 总被引:16,自引:0,他引:16
Fain JN Cheema PS Bahouth SW Lloyd Hiler M 《Biochemical and biophysical research communications》2003,300(3):674-678
Resistin, also known as Fizz3 or ADSF, is a protein found in murine adipose tissue and inflammatory lung exudates. The present studies found that resistin was released by explants of human adipose tissue but the release was quite variable ranging from 3 to 158 ng/g over 48 h. The release of resistin was 250% greater by explants of omental than by explants of human subcutaneous abdominal adipose tissue. Resistin release by adipocytes was negligible as compared to that by the non-fat cells of adipose tissue. Leptin formation by adipocytes was 8-fold greater than its formation by the non-fat cells, while the formation of PAI-1 by adipocytes was 38% of that by the non-fat cells. The conversion of glucose to lactate as well as the formation of PGE(2) and IL-8 was approximately 15% of that by the non-fat cells. In contrast the release of IL-6 and IL-1beta by adipocytes was 4-7% of that by the non-fat cells while the formation of resistin and IL-10 by adipocytes was 2% of that by non-fat cells. The release of adiponectin by explants ranged from 1000 to 5000 ng/g over 48 h but did not correlate with that of resistin. The present data suggest that resistin release by explants of human adipose tissue in primary culture is largely derived from the non-fat cells present in the explants. 相似文献
7.
In the epididymal portion of rat vas deferens, facilitation of noradrenaline release mediated by adenosine A2A receptors, but not that mediated by beta2-adrenoceptors or by direct activation of adenylyl cyclase, was attenuated by blockade of alpha2-adrenoceptors and abolished by simultaneous blockade of alpha2-adrenoceptors, adenosine A1 and P2Y receptors. The adenosine A2A receptor-mediated facilitation was not changed by inhibitors of protein kinase A, protein kinase G or calmodulin kinase II but was prevented by inhibition of protein kinase C with chelerythrine or bisindolylmaleimide XI. Activation of protein kinase C with phorbol 12-myristate 13-acetate caused a facilitation of noradrenaline release that was abolished by bisindolylmaleimide XI and reduced by antagonists of alpha2-adrenoceptors, adenosine A1 and P2Y receptors. Activation of adenosine A2A receptors attenuated the inhibition of noradrenaline release mediated by the presynaptic inhibitory receptors. This effect was mimicked by phorbol 12-myristate 13-acetate and prevented by bisindolylmaleimide XI. It is concluded that adenosine A2A receptors facilitate noradrenaline release by a mechanism that involves a protein kinase C-mediated attenuation of effects mediated by presynaptic inhibitory receptors, namely alpha2-adrenoceptors, adenosine A1 and P2Y receptors. 相似文献
8.
Metabolite-controlled phosphorylation of hepatic phosphofructokinase proceeds by cAMP-dependent protein kinase 总被引:1,自引:0,他引:1
In hepatocytes 32P-incorporation into rat liver phosphofructokinase is stimulated by glucose as well as by glucagon, the effects of both stimuli being prevented by L-alanine [Eur. J. Biochem. (1982) 122, 175]. The phosphopeptides of the enzyme derived from limited proteolysis by subtilisin and from exhaustive tryptic digestion were analyzed either by one-dimensional mapping on sodium dodecyl sulphate-polyacrylamide slab gels and by fingerprint mapping, respectively. It is shown that in vivo stimulation of 32P-incorporation by glucose or by glucose plus glucagon results in identical phosphopeptide maps, and that these maps were identical with those obtained from phosphofructokinase phosphorylated in vitro with catalytic subunit of cAMP-dependent protein kinase. It is concluded that in the intact liver cell phosphofructokinase is phosphorylated by cAMP-dependent protein kinase but that the state of phosphorylation is modified by metabolite control. 相似文献
9.
γ-Irradiation combined with freeze-thawing, i.e. irradiation followed by freeze-thawing and freeze-thawing followed by irradiation, was applied to prepare poly(vinyl alcohol) (PVA)/water soluble chitosan (ws-chitosan) hydrogels for wound dressing. The properties of these hydrogels were investigated and compared to those prepared by freeze-thawing and by irradiation, respectively. Hydrogels made by irradiation followed by freeze-thawing show larger swelling capacity and mechanical strength, higher thermal stability, lower water evaporation rate, and are less turbid than those made by pure freeze-thawing and freeze-thawing followed by irradiation. Hydrogels made by irradiation alone cannot be used as wound dressing due to their poor mechanical strength. SEM results show that the final structure of hydrogels made by combined irradiation and freeze-thawing is mainly determined by the first processing step. It is found that the appropriate amount of ws-chitosan can endow hydrogels with large swelling capacity and mechanical strength. The presence of ws-chitosan provides the hydrogels with good antibacterial activity against Escherichia coli (E. coli). 相似文献
10.
植物生长调节物质IP-1号对木薯产量及其生物性状的影响 总被引:4,自引:0,他引:4
1990和1991年在木薯(ManihotesculentaCrantz)生长期以植物生长调节物质IP-1号0,20,30和40ppm进行叶面喷洒,结果表明:30ppm处理可使木薯块根产量平均增加54.44%,块根淀粉含量平均提高20.81%。单株最大薯重提高31.55%,块根数增加21.17%,块根长度增长17.62%,地上部鲜重增加34.36%,植株高度增加4.36%,植株收获期保留青叶数增加19.42%,主茎直径增加6.26%,块根直径增加2.58%,叶片的叶绿素和蛋白质含量分别提高5.57%和25.96%,叶片光合作用强度提高15.86%,而对主茎高度、主茎节数没有明显影响。 相似文献
11.
Mohammad D. Bazzi Paul D. Lampe Gale M. Strasburg Gary L. Nelsestuen 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1987,931(3)
The mechanism by which calmodulin and troponin C influence phosphorylation of troponin I (TnI) by protein kinase C was investigated. The phosphorylation of TnI by protein kinase C requires the presence of acidic phospholipid, calcium and diacylglycerol. Light scattering intensity and fluorescence intensity experiments showed that TnI associated with the phospholipid membranes and caused extensive aggregation. In the presence of Ca2+, TnI-phospholipid interactions were prevented by approximately stoichiometric amounts of either troponin C or calmodulin. Troponin C was shown to completely inhibit phosphorylation of TnI by either protein kianse C or by phosphorylase b kinase. In contrast, calmodulin completely inhibited phosphorylation of TnI by protein kinase C, but had only little effect on TnI phosphorylation by phosphorylase b kinase. Inhibition by calmodulin did not appear to be due to interaction with PKC, since calmodulin mildly increased protein kinase C phosphorylation of histone III-S. The ratio of phosphoserine to phosphothreonine in protein kinase C-phosphorylated TnI remained approximately constant for reactions inhibited by up to 90% by clamodulin. TnI interactions with phospholipid and phosphorylation of TnI by PKC were also prevented by high salt concentrations. However, salt concentrations adequate to inhibit phosphorylation were sufficient to dissociate only TnI, but not protein kinase C from the membrane. These results suggest that the binding of TnI to phospholipid is required for phosphorylation by protein kinase C and that prevention of this binding by any means completely inhibited phosphorylation of TnI by protein kinase C. 相似文献
12.
目的比较ELISA(enzyme-linked immunosorbent assay)、IFA(immuno-fluorescence assay)和WB(Western blot)三种方法在大鼠仙台病毒血清学检测中的差异。方法仙台病毒蛋白抗原经凝胶电泳分离转移后用于血清学检测的WB方法;使用IFA、ELISA方法对20份无菌大鼠、227份SPF大鼠以及63份清洁级大鼠送检血清样品进行检测,阳性及可疑样品用WB方法进行了验证。结果 20份无菌大鼠血清样品被3种方法检测为仙台病毒抗体阴性;SPF级大鼠样品被IFA方法判定为阴性,1.32%(3/227)被ELISA方法判定为阳性,其中有2/3被WB确认为阳性;ELISA、IFA和WB在清洁级大鼠样品中检出仙台病毒的阳性率分别为为18.12%、11.34%和15.87%。结论三种检测方法灵敏度从高到低依次为ELISA、WB和IFA。WB方法可作为IFA和ELISA难以确定结果的替代方法。 相似文献
13.
啤酒酵母生产的重组水蛭素的纯化及脱色 总被引:5,自引:0,他引:5
对啤酒酵母生产的重组水蛭素变异体1(rHV1)进行多步骤的纯化。首先将分泌到培养上清液中的水蛭素进行硫酸铵沉淀和SephadexG-50凝胶过滤,再用Q-SepharoseHP阴离子交换层析分离,最后用HPLCSP-5PW阳离子交换柱脱色及HPLCC8柱反相层析。真空干燥后得到的水蛭素蛋白经SPS-PAGE、N端氨基酸序列分析、抗凝血酶活力分析鉴定,证明已获得高纯度的重组水蛭素HV1制剂,为利用基因工程方法生产重组水蛭素的规模化生产及临床应用提供了依据 相似文献
14.
Stimulation of cyclic AMP (cAMP) accumulation in rat cortex slices by 1 microM forskolin (F) was markedly reduced (96%) by treatment with adenosine deaminase (ADA). The effect of ADA was progressively less at higher concentrations of F, but still inhibited the response by 50% at 100 microM F. ADA-mediated inhibition of the cAMP response to 1 microM F was completely reversed by 5 microM 2-chloroadenosine (CA), an ADA-resistant analogue. Stimulation by F (controls) and F plus CA (ADA treated) in cortex slices was significantly inhibited by 200 microM caffeine (CAF) and by 10 microM 8-phenyltheophylline. cAMP accumulation in ADA-treated cortex slices stimulated with CA at concentrations from 5 to 100 microM was markedly enhanced by 1 microM F. Neither ADA treatment nor 200 microM CAF significantly affected cAMP accumulation in slices stimulated by 1 microM vasoactive intestinal polypeptide or adenylate cyclase in membranes stimulated by 1 microM F. CAF (1 mM) did not significantly increase basal cAMP levels in cortex slices, whereas 1 mM 3-isobutyl-1-methylxanthine caused a significant 80% increase and 100 microM rolipram enhanced cAMP levels by 4.5-fold. F-stimulated cAMP accumulation (1 microM) in cortex slices was inhibited 98% by 1 mM CAF and 49% by 1 mM 3-isobutyl-1-methylxanthine, and was enhanced 2.5-fold by 100 microM rolipram. These data have been interpreted to indicate that the stimulation of cAMP accumulation in rat cortex slices by 1 microM F is predominantly due to synergistic interaction with endogenous adenosine and that the inhibition of this response by CAF is largely due to blockade of adenosine receptors. 相似文献
15.
MuhammadFAROOQ S.M.A.BASRA NazirAHMAD K.HAFEEZ 《植物学报(英文版)》2005,47(2):187-193
In a laboratory study, indica and japonica rice (Oryza sativa L.) seeds were exposed to thermal hardening (heating followed by chilling followed by heating; chilling followed by heating followed by chilling; heating followed by chilling or chilling followed by heating). In indica rice, heating followed by chilling followed by heating resulted in decreased mean germination time, time to start germination, electrical conductivity of seed leachates, and time to 50% germination, as well as increased germination index, energy of germination, radicle and plumule length, root length, root/shoot ratio, root fresh and dry weight, radicle and plumule growth rate, and shoot fresh weight. In japonica rice, chilling followed by heating followed by chilling performed better than all other treatments, including control. 相似文献
16.
Comparative study of antioxidants as quenchers or scavengers of reactive oxygen species based on quenching of MCLA-dependent chemiluminescence. 总被引:1,自引:0,他引:1
The quenching or scavenging effect of non-enzymatic antioxidants against reactive oxygen species (ROS) was studied by comparing the degree of suppression of chemiluminescence (CL) caused by the oxidation of MCLA (methoxylated Cypridina luciferin analogue) by ROS. MCLA-dependent CL caused by O2- was effectively quenched by ascorbic acid, beta-carotene, lycopene and astaxanthin, while it was enhanced by alpha-tocopherol. The CL by 1O2 was quenched effectively by beta-carotene, lycopene and astaxanthin, moderately by ascorbic acid, and slightly by alpha-tocopherol. beta-Carotene and alpha-tocopherol remarkably suppressed the CL when ROS was HO*. The present study revealed that MCLA-dependent CL assay provides a simple and rapid method for the evaluation of antioxidants as a quencher or scavenger against any kind of ROS. 相似文献
17.
Campylobacter jejuni isolates obtained from human and animal sources showed different invasion levels into human embryonic intestinal (INT-407) cells. There was no significant relation between the degree of invasion and cytotoxins production. The depolymerization of both microfilaments by cytochalasin-D and microtubules by colchicine, demecolcine and nocodazole or stabilization of microtubules by paclitaxel reduced the invasiveness of C. jejuni, although microfilament depolymerization showed greater inhibition than microtubule depolymerization. Interference with receptor-mediated endocytosis by G-strophanthin and monodansylcadaverine and inhibition of endosome acidification by monensin reduced the number of viable intracellular C. jejuni cells. Furthermore inhibition of only host protein kinases by staurosporine, but not phosphoinositide 3-kinase by wortmannin or protein kinase-C by calphostin-C, significantly reduced invasion of epithelial cells by C. jejuni. These data suggest that the internalization mechanism triggered by C. jejuni is strikingly different from the microfilament-dependent invasion mechanism exhibited by many of the well-studied enteric bacteria such as enteroinvasive strains of Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersinia enterocolitica and Yersinia pseudotuberculosis. 相似文献
18.
C.A. Pasternak G.M. Alder C.L. Bashford Y.E. Korchev C. Pederzolli T.K. Rostovtseva 《FEMS microbiology letters》1992,105(1-3):83-92
Abstract Common features in the induction of pores by various agents are as follows: induction is stochastic and progressive; damage by different agents is often synergistic and limited. The prevention of membrane damage is affected by trivalent and divalent cations, by low pH, by low ionic strength and by high osmotic pressure. The inhibitory role of protons and divalent cations is considered in greater detail: pore-forming agents can be classified into two groups: channels across planar lipid bilayers induced by the first group display voltage-sensitive, reversible inhibition by divalent cations; channels of the second group show voltage-insensitive, irreversible inhibition by divalent cations. A search for the ligands to which divalent cations and protons bind has proved elusive. Comparison with the phenomenon of 'surface conductance' through narrow apertures, that is manifest in the absence of any pore-forming agent, may prove fruitful. 相似文献
19.
Global warming has changed the distributions of forests of northeastern China. Larix are very important species in this area. Predicting the potential distributions of Larix species and their responses to climate change would attract more and more attention.This paper predicted the potential distributions of three Larix species based on 'climatic-topographic' relationships by logistic regression. The results showed that L. gmelinii is predicted to retreat northwestward by 220 km by 2050 and by 270 km more by 2100; L. olgensis var. changpaiensis is predicted to retreat northwestward by 200 km by 2050 and by 190 to 300 km more by 2100; L. principis-rupprechtii is predicted to retreat northeastward by 200 km by 2050 and by 250 to 400 km more by 2100. This indicated that L. gmelinii could have its optimum latitude moved into Russia, L. olgensis var. changpaiensis could move to the Small Xing'an Mountains and L. principis-rupprechtii would move to the middle part of the Great Xing'an Mountains. 相似文献
20.
The autoantigen Ro52 is an E3 ubiquitin ligase that can ubiquitinate itself (self-ubiquitination). Recently, we showed that UnpEL/Usp4 is an isopeptidase that can deconjugate ubiquitin from self-ubiquitinated Ro52. Here, we showed that UnpEL is ubiquitinated by Ro52 in cooperation with UbcH5B in vitro. We also showed that UnpEL is ubiquitinated by Ro52 in HEK293T cells. Interestingly, a catalytically inactive UnpEL mutant was strongly ubiquitinated by Ro52 in HEK293T cells. These results suggest that wild-type UnpEL is ubiquitinated by Ro52 and deubiquitinated by itself (self-deubiquitination), while mutant UnpEL is ubiquitinated by Ro52 but not deubiquitinated by itself. In conclusion, Ro52 and UnpEL transregulate each other by ubiquitination and deubiquitination. 相似文献