Karyotype and Sex Chromosomes in Cephalotaxus sinensis

In Cephalotascus sinensis (Rehd. et Wils. ) Li the somatic chromosome number was found to be of 2n=24. Eleven pairs of chromosomes possessed their centromeres at median or median-submedian regions. The shortest pair of chromosomes was the SAT chromosome which possessed their centromeres at the submedian regions. The sex chromosomes were demonstrated by the Giemsa C-banding technique. The sexual determination mechanism of female was WZ type (2n=24=22A+WZ), and that of male was ZZ type (2n=24=22A +ZZ).     

肥牛木根化学成分研究

从肥牛木(Cephalomappa sinensis)根的乙醇提取物中分离得到了7个化合物,经波谱分析确定其结构分别为:羽扇豆-20(29)-烯-2α,3β-二醇(1),苏门树脂醇酸(2),油桐酸(3),3-βstigmast-5-en-3-O--βD-xylopyranoside(4),东莨菪内酯(5),香草酸(6),油酸(7)。以上化合物均为首次从该植物中分离得到。     

云南苦绳化学成分的研究

采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备型HPLC等方法从云南苦绳中分离纯化了6种化学成分。通过红外光谱、一维核磁共振波谱、二维核磁共振波谱、质谱等方法确定其化学结构为二十烷酸(1)、α,γ-二棕榈酸甘油脂(2)、β-谷甾醇(3)、4ɑ-甲基-胆甾-7-烯-3-β-醇(4)、3,4’-二甲氧基-4,9,9’-三羟基-苯并呋喃木脂素-7’-烯(5)、松脂素(6)。化合物1～6均为首次从云南苦绳根茎中分离得到。     

华钩藤化学成分研究

从华钩藤(Uncaria sinensis(Oliv.) Havil)的乙醇溶液中分离得到8个化合物,经波谱学方法并与已知样品对照段鉴定为:β-谷甾醇(1)、胡萝卜苷(2)、翅果定碱(3)、乌苏酸(4)、丁香色原酮甙Ⅰ(5)、美商陆甙A(6)、东莨菪素(7)和乌苏醛(8).其中化合物5和6为首次从该植物中分离得到.     

冬虫夏草(Cordyceps sinensis)菌丝体固体发酵粉化学成分的分析

本研究对一种冬虫夏草(Cordyceps sinensis)菌丝体固体发酵粉的化学成份进行了分析,包括粗成份,生物活性成份,脂肪酸,氨基酸,金属元素等。冬虫夏草菌丝体固体发酵粉中3’-脱氧腺苷含量40．81μg/g,腺苷含量745．4μg/g;甘露醇的含量为165．8mg／g,多糖含量为157．3mg／g;谷氨酸,天门冬氨酸,苯丙氨酸是三大类主要氨基酸;不饱和脂肪酸是优势脂肪酸,以亚油酸,油酸为主。和文献记录的结果相比,虫草发酵粉中3’-脱氧腺苷,甘露醇的含量明显高于冬虫夏草子实体中相应成份的含量;各种氨基酸的含量和水平与冬虫夏草子实体中相应氨基酸的含量和水平相当;虫草发酵粉中的其它化学成份和冬虫夏草子实体中相应成份基本一致。     

A Study on Bisflavone Constituents and Chemotaxonomy of Cephalotaxus

Bisflavone constituents of 6 species in the genus Cephalotaxus were analysed by different chemical methods. The result and some suggestions are as follows: 1. Fu's (1984) division of the genus Cephalotaxus into two sections, Cephalotaxus and Pectinatea, is supported by the fact that oliveriflavone has so far been found only in Cephalotaxus oliveri. 2. Our result is favourable to the opinion that Cephalotaxaceae is a natural taxon and includes only one genus, Cephalotaxus, but not to the placement of Amentotaxus of Taxaceae into Cephalotaxaceae. Accroding to the literature and our analytical data, bisflavones and alkaloids are the main constituents of Cephalotaxus, which have not been found in Amentotaxus of Taxaceae. 3. Cephalotaxus is considered closely related to Taxaceae, and Podocarpaceae, but not closely related to Araucariaceae, Pinaceae, Taxodiaceae and Cupressaceae. 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皂荚化学组成的研究

初步研究了皂荚中所含的化学成分,测定了皂甙、纤维素、半纤维素、木质素、果胶、蛋白质相对于绝干皂荚的质量百分含量。结果表明,皂荚中含有鞣质、酚性物质、生物碱、有机酸、糖类、油脂等物质。     

湖北小连翘中化学成分的高效液相色谱与电喷雾离子化质谱联用分析

湖北小连翘(Hypericum hubeiense L.H.Wu et D.P.Yang)是2004年在我国发现的一个金丝桃属(Hypericum)植物(藤黄科)新种.利用高效液相色谱与质谱联用技术对该植物中主要化学成分进行了分离分析,初步鉴定出金丝桃素类、黄酮类等12种化学成分.同时,以金丝桃素为外标,采用HPLC法在590 nm波长下测定了金丝桃素类成分的含量.     

Studies on the Chemical Constituents of Bretschneidera sinensis Hemsl. EndEmic in China

Four compounds were for the first time isolated from trunk of Bretschneidera sinensis Hemsl. grown in Yunnan. They were identified as 3-epi-betulinic acid(l), 3,5,7-tri-hydroxy-flavanonol (pinobanksin) (2), daucosterol (3) and β-sitosterol (4), respectively by chemical and spectral (IR, NMR, and MS) analyses. 1 and 2 were the main components.     

云南翠雀花化学成分的测定

采用粗纤维法、凯氏定氮法、斐林试剂法、索氏浸提法、干灰化法和重量法等多种化学分析方法对云南翠雀花的化学成分进行了分析.结果显示,云南翠雀花含有丰富的维生素、矿质元素以及异亮氨酸、蛋氨酸、赖氨酸等17种氨基酸,其中粗纤维、VB2和蛋氨酸含量尤为丰富,分别为32.83％、3.26 mg·g-1、3.27 mg· g-1.