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1.
QTLs for agronomic traits from a Mediterranean barley progeny grown in several environments 总被引:4,自引:2,他引:2
B. Teulat O. Merah I. Souyris D. This 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(5):774-787
In order to identify quantitative trait loci (QTLs) controlling agronomic trait variation and their consistency under Mediterranean
conditions in barley, a progeny of 167 recombinant inbred lines (RILs) and the parents Tadmor and Er/Apm, originating from
the Mediterranean basin, were grown under Mediterranean conditions in 1995, 1996, 1997 and 1999. For the 2 first years (M95
and G96), one replicate was grown, but for the latter (M97 and M99) two rainfed (rain) and two irrigated (ir) replicates were
produced. M95, G96, M97rain, M97ir, M99rain and M99ir were considered as six different environments and were compared in terms
of their meteorological conditions and water supply. Grain yield and yield components were assessed, as well as heading date
and plant height. Highly significant differences were noted between environments. QTLs were obtained from each environment
separately and from a multiple environment analysis (simple interval mapping and simplified composite interval mapping). Despite
heterogeneity between environments, numerous QTLs were common to several environments. This was particularly true for traits
like plant height and thousand-grain weight. The most reliable QTLs which explained the largest part of the phenotypic variation
were obtained for plant height on chromosomes 3 (3H) and 6 (6H). The multiple-environment analysis provided an opportunity
to identify consistent QTLs for agronomic traits over six Mediterranean environments. A total of 24 consistent QTLs were detected.
Out of these, 11 presented main effects, seven presented QTL×E interaction, and six presented both effects. In addition, 18
of the consistent QTLs were common to other published work and six seemed specific to this study. These latter QTLs could
be involved in Mediterranean adaptive specificities or could be specific to the studied genetic background. Finally, when
the rainfed and the irrigated environments of M97 were considered separately, a total of 16 QTLs presenting main effects over
the two water conditions were identified, whereas five QTLs seemed dependent on the water conditions.
Received: 31 January 2001 / Accepted: 19 February 2001 相似文献
2.
Yadav RS Bidinger FR Hash CT Yadav YP Yadav OP Bhatnagar SK Howarth CJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(3):512-520
A mapping population of 104 F(3) lines of pearl millet, derived from a cross between two inbred lines H 77/833-2 x PRLT 2/89-33, was evaluated, as testcrosses on a common tester, for traits determining grain and stover yield in seven different field trials, distributed over 3 years and two seasons. The total genetic variation was partitioned into effects due to season (S), genotype (G), genotype x season interaction (G x S), and genotype x environment-within-season interaction [G x E(S)]. QTLs were determined for traits for their G, G x S, and G x E(S) effects, to assess the magnitude and the nature (cross over/non-crossover) of environmental interaction effects on individual QTLs. QTLs for some traits were associated with G effects only, while others were associated with the effects of both G and G x S and/or G, G x S and G x E(S) effects. The major G x S QTLs detected were for flowering time (on LG 4 and LG 6), and mapped to the same intervals as G x S QTLs for several other traits (including stover yield, harvest index, biomass yield and panicle number m(-2)). All three QTLs detected for grain yield were unaffected by G x S interaction however. All three QTLs for stover yield (mapping on LG 2, LG 4 and LG 6) and one of the three QTLs for grain yield (mapping on LG 4) were also free of QTL x E(S) interactions. The grain yield QTLs that were affected by QTL x E(S) interactions (mapping on LG 2 and LG 6), appeared to be linked to parallel QTL x E(S) interactions of the QTLs for panicle number m(-2) on (LG 2) and of QTLs for both panicle number m(-2) and harvest index (LG 6). In general, QTL x E(S) interactions were more frequently observed for component traits of grain and stover yield, than for grain or stover yield per se. 相似文献
3.
Impact of drought on productivity and water use efficiency in 29 genotypes of Populus deltoides x Populus nigra 总被引:1,自引:0,他引:1
Monclus R Dreyer E Villar M Delmotte FM Delay D Petit JM Barbaroux C Le Thiec D Bréchet C Brignolas F 《The New phytologist》2006,169(4):765-777
We examined the relationships among productivity, water use efficiency (WUE) and drought tolerance in 29 genotypes of Populus x euramericana (Populus deltoides x Populus nigra), and investigated whether some leaf traits could be used as predictors for productivity, WUE and drought tolerance. At Orléans, France, drought was induced on one field plot by withholding water, while a second plot remained irrigated and was used as a control. Recorded variables included stem traits (e.g. biomass) and leaf structural (e.g. leaf area) and functional traits [e.g. intrinsic water use efficiency (Wi) and carbon isotope discrimination (Delta)]. Productivity and Delta displayed large genotypic variability and were not correlated. Delta scaled negatively with Wi and positively with stomatal conductance under moderate drought, suggesting that the diversity for Delta was mainly driven by stomatal conductance. Most of the productive genotypes displayed a low level of drought tolerance (i.e. a large reduction of biomass), while the less productive genotypes presented a large range of drought tolerance. The ability to increase WUE in response to water deficit was necessary but not sufficient to explain the genotypic diversity of drought tolerance. 相似文献
4.
C. Frova P. Krajewski N. di Fonzo M. Villa M. Sari-Gorla 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):280-288
Grain yield is a complex trait, strongly influenced by the environment: severe losses can be caused by drought, a stress common
in most maize-growing areas, including temperate climatic zones. Accordingly, drought tolerance is one of the main components
of yield stability, and its improvement is a major challenge to breeders. The aim of the present work was the identification,
in maize genotypes adapted to temperate areas, of genomic segments responsible for the expression of drought tolerance of
yield components: ear length, ear weight, kernel weight, kernel number and 50-kernel weight. A linkage analysis between the
expression of these traits and molecular markers was performed on a recombinant inbred population of 142 families, obtained
by repeated selfing of the F1 between lines B73 and H99. The population, genotyped at 173 loci (RFLPs, microsatellites and AFLPs), was evaluated in well-watered
and water-stressed conditions. A drought tolerance index was calculated as the ratio between the mean value of the trait in
the two environments. For the traits measured, a highly positive correlation was found over the two water regimes, and more
than 50% of the quantitative trait loci (QTLs) detected were the same in both; moreover, the direction of the allelic contribution
was always consistent, the allele increasing the trait value being mostly from line B73. Several QTLs were common to two or
more traits. For the tolerance index, however, most of the QTLs were specific for a single component and different from those
controlling the basic traits; in addition, a large proportion of the alleles increasing tolerance were provided by line H99.
The data suggest that drought tolerance for yield components is largely associated with genetic and physiological factors
independent from those determining the traits per se. The implications of these results for developing an efficient strategy of marker-assisted selection for drought tolerance
are discussed.
Received: 19 October 1998 / Accepted: 28 December 1998 相似文献
5.
Quantitative trait loci influencing drought tolerance in grain sorghum (Sorghum bicolor L. Moench) 总被引:7,自引:0,他引:7
H. Kebede P. K. Subudhi D. T. Rosenow H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):266-276
Drought is a major constraint in sorghum production worldwide. Drought-stress in sorghum has been characterized at both pre-flowering
and post-flowering stages resulting in a drastic reduction in grain yield. In the case of post-flowering drought stress, lodging
further aggravates the problem resulting in total loss of crop yield in mechanized agriculture. The present study was conducted
to identify quantitative trait loci (QTLs) controlling post-flowering drought tolerance (stay green), pre-flowering drought
tolerance and lodging tolerance in sorghum using an F7 recombinant inbred line (RIL) population derived from the cross SC56×Tx7000. The RIL lines, along with parents, were evaluated
for the above traits in multiple environments. With the help of a restriction fragment length polymorphism (RFLP) map, which
spans 1,355 cM and consists of 144 loci, nine QTLs, located over seven linkage groups were detected for stay green in several
environments using the method of composite interval mapping. Comparison of the QTL locations with the published results indicated
that three QTLs located on linkage groups A, G and J were consistent. This is considered significant since the stay green
line SC56 used in our investigation is from a different source compared to B35 that was used in all the earlier investigations.
Comparative mapping has shown that two stay green QTLs identified in this study corresponded to stay green QTL regions in
maize. These genomic regions were also reported to be congruent with other drought-related agronomic and physiological traits
in maize and rice, suggesting that these syntenic regions might be hosting a cluster of genes with pleiotropic effects implicated
in several drought tolerance mechanisms in these grass species. In addition, three and four major QTLs responsible for lodging
tolerance and pre-flowering drought tolerance, respectively, were detected. This investigation clearly revealed the important
and consistent stay green QTLs in a different stay green source that can logically be targeted for positional cloning. The
identification of QTLs and markers for pre-flowering drought tolerance and lodging tolerance will help plant breeders in manipulating
and pyramiding those traits along with stay green to improve drought tolerance in sorghum.
Received: 2 June 2000 / Accepted: 15 November 2000 相似文献
6.
To investigate the genetic basis of drought tolerance in soybean ( Glycine max L. Merr.) a recombinant inbred population with 184 F2:7:11 lines developed from a cross between Kefeng1 (drought tolerant) and Nannong1138-2 (drought sensitive) were tested under water-stressed and well-watered conditions in field and greenhouse trials. Traits measured included leaf wilting coefficient, excised leaf water loss and relative water content as indicators of plant water status and seed yield. A total of 40 quantitative trait loci (QTLs) were identified: 17 for leaf water status traits under drought stress and 23 for seed yield under well-watered and drought-stressed conditions in both field and greenhouse trials. Two seed yield QTLs were detected under both well-watered and drought-stressed conditions in the field on molecular linkage group H and D1b, while two seed yield QTLs on molecular linkage group C2 were found under greenhouse conditions. Several QTLs for traits associated with plant water status were identified in both field and greenhouse trials, including two leaf wilting coefficient QTLs on molecular linkage group A2 and one excised leaf water loss QTL on molecular linkage group H. Phenotypic correlations of traits suggested several QTLs had pleiotropic or location-linked associations. These results will help to elucidate the genetic basis of drought tolerance in soybean, and could be incorporated into a marker-assisted selection breeding program to develop high-yielding soybean cultivars with improved tolerance to drought stress. 相似文献
7.
Genetic analysis for drought resistance of rice at reproductive stage in field with different types of soil 总被引:9,自引:0,他引:9
Yue B Xiong L Xue W Xing Y Luo L Xu C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(6):1127-1136
Drought resistance of rice is a complex trait and is mainly determined by mechanisms of drought avoidance and drought tolerance. The present study was conducted to characterize the genetic basis of drought resistance at reproductive stage in field by analyzing the QTLs for drought response index (DRI, normalized by potential yield and flowering time), relative yield, relative spikelet fertility, and four traits of plant water status and their relationships with root traits using a recombinant inbred population derived from a cross between an indica rice and upland rice. A total of 39 QTLs for these traits were detected with individual QTL explained 5.1–32.1% of phenotypic variation. Only two QTLs for plant water status were commonly detected in two environments, suggesting different mechanisms might exist in two types of soil conditions. DRI has no correlation with potential yield and flowering time under control, suggesting that it can be used as a good drought resistance index in field conditions. The co-location of QTLs for canopy temperature and delaying in flowering time suggested a usefulness of these two traits as indexes in drought resistance screening. Correlation and QTL congruence between root traits and putative drought tolerance traits revealed that drought avoidance (via thick and deep root traits) was the main genetic basis of drought resistance in sandy soil condition, while drought tolerance may play more role in the genetic basis of drought resistance in paddy soil condition. Therefore, both drought mechanisms and soil textures must be considered in the improvement of drought resistance at reproductive stage in rice. 相似文献
8.
Z.F. Hao X.H. Li C.X. Xie M.S. Li D.G. Zhang L. Bai & S. H. Zhang 《The Annals of applied biology》2008,153(1):73-83
Unravelling the molecular basis of drought tolerance will provide novel opportunities for improving crop yield under water-limited conditions. The present study was conducted to identify quantitative trait loci (QTLs) controlling anthesis–silking interval (ASI), ear setting percentage (ESP) and grain yield (GY). The mapping population included 234 F2 plants derived from the cross X178 (drought tolerant) × B73 (drought susceptible). The corresponding F2:3 progenies, along with their parents, were evaluated for the above-mentioned traits under both well-watered and water-stressed field conditions in three different trials carried out in central and southern China. Interval mapping and composite interval mapping identified 45 and 65 QTLs for the investigated traits, respectively. Two QTL clusters influencing ASI and ESP on chromosomes 1 (bin 1.03) and 9 (bins 9.03–9.05) were identified in more than two environments, showing sizeable additive effects and contribution to phenotypic variance; these two QTL clusters influenced GY only in one environment. No significant interaction was detected between the two genomic regions. A comparative analysis of these two QTL clusters with the QTLs controlling maize drought tolerance previously described in three mapping populations confirmed and extended their relevance for marker-assisted breeding to improve maize production under water-limited conditions. 相似文献
9.
Drought stress has long been a major constraint in maintaining yield stability of soybean (Glycine max (L.) Merr.) in rainfed ecosystems. The identification of consistent quantitative trait loci (QTL) involving seed yield per plant (YP) and drought susceptibility index (DSI) in a population across different environments would therefore be important in molecular marker-assisted breeding of soybean cultivars suitable for rainfed regions. The YP of a recombinant line population of 184 F2:7:11 lines from a cross of Kefengl and Nannong1138-2 was studied under water-stressed (WS) and well-watered (WW) conditions in field (F) and greenhouse (G) trials, and DSI for yield was calculated in two trials. Nineteen QTLs associated with YP-WS and YP-WW, and 10 QTLs associated with DSI, were identi- fied. Comparison of these QTL locations with previous findings showed that the majority of these regions control one or more traits re- lated to yield and other agronomic traits. One QTL on molecular linkage group (MLG) K for YP-F, and two QTLs on MLG C2 for YP-G, remained constant across different water regimes. The regions on MLG C2 for YP-WW-F and MLG H for YP-WS-F had a pleiotropic effect on DSI-F, and MLG A1 for YP-WS-G had a pleiotropic effect on DSI-G. The identification of consistent QTLs for YP and DSI across different environments will significantly improve the efficiency of selecting for drought tolerance in soybean. 相似文献
10.
Yadav RS Hash CT Bidinger FR Cavan GP Howarth CJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):67-83
Drought stress during the reproductive stage is one of the most important environmental factors reducing the grain yield and
yield stability of pearl millet. A QTL mapping approach has been used in this study to understand the genetic and physiological
basis of drought tolerance in pearl millet and to provide a more-targeted approach to improving the drought tolerance and
yield of this crop in water-limited environments. The aim was to identify specific genomic regions associated with the enhanced
tolerance of pearl millet to drought stress during the flowering and grain-filling stages. Testcrosses of a set of mapping-population
progenies, derived from a cross of two inbred pollinators that differed in their response to drought, were evaluated in a
range of managed terminal drought-stress environments. A number of genomic regions were associated with drought tolerance
in terms of both grain yield and its components. For example, a QTL associated with grain yield per se and for the drought
tolerance of grain yield mapped on linkage group 2 and explained up to 23% of the phenotypic variation. Some of these QTLs
were common across stress environments whereas others were specific to only a particular stress environment. All the QTLs
that contributed to increased drought tolerance did so either through better than average maintenance (compared to non-stress
environments) of harvest index, or harvest index and biomass productivity. It is concluded that there is considerable potential
for marker-assisted backcross transfer of selected QTLs to the elite parent of the mapping population and for their general
use in the improvement of pearl millet productivity in water-limited environments.
Received: 15 November 2000 / Accepted: 12 April 2001 相似文献
11.
Rainer Messmer Yvan Fracheboud Marianne Bänziger Mateo Vargas Peter Stamp Jean-Marcel Ribaut 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(5):913-930
A recombinant inbred line (RIL) population was evaluated in seven field experiments representing four environments: water
stress at flowering (WS) and well-watered (WW) conditions in Mexico and Zimbabwe. The QTLs were identified for each trait
in each individual experiment (single-experiment analysis) as well as per environment, per water regime across locations and
across all experiments (joint analyses). For the six target traits (male flowering, anthesis-to-silking interval, grain yield,
kernel number, 100-kernel fresh weight and plant height) 81, 57, 51 and 34 QTLs were identified in the four step-wise analyses,
respectively. Despite high values of heritability, the phenotypic variance explained by QTLs was reduced, indicating epistatic
interactions. About 80, 60 and 6% of the QTLs did not present significant QTL-by-environment interactions (QTL × E) in the
joint analyses per environment, per water regime and across all experiments. The expression of QTLs was quite stable across
years at a given location and across locations under the same water regime. However, the stability of QTLs decreased drastically
when data were combined across water regimes, reflecting a different genetic basis of the target traits in the drought and
well-watered trials. Several clusters of QTLs for different traits were identified by the joint analyses of the WW (chromosomes
1 and 8) and WS (chromosomes 1, 3 and 5) treatments and across water regimes (chromosome 1). Those regions are clear targets
for future marker-assisted breeding, and our results confirm that the best approach to breeding for drought tolerance includes
selection under water stress. 相似文献
12.
以旱稻品种IRAT109与水稻品种越富杂交构建的DH群体的116个株系及其亲本为材料,在水、旱2种栽培条件下种植,研究了稻米蒸煮和营养品质性状的变化规律,在水、旱2个土壤水分环境下对直链淀粉含量(AC)、胶稠度(GC)、碱消值(GT)和蛋白质含量(PC)4个蒸煮和营养品质性状进行QTL定位及QTLs与环境互作分析。结果表明,以上4个品质性状在水、旱2种不同栽培条件下差异较大,说明这些性状受水分条件影响较大,旱栽条件下稻米蒸煮和营养各品质性状均有不同程度的升高,其中蛋白质含量平均提高37.9%。QTL分析结果表明,4个稻米品质性状在2个环境中的表现型值都为连续分布,均存在超亲遗传类型,共检测到7个加性效应QTL与稻米蒸煮和营养品质性状4项指标有关,分别位于第1、2、3、6、8、11染色体上,单个QTLs对性状的贡献率在1.91%~19.77%之间。位于第3染色体上控制碱消值的QGt3,第6染色体上控制直链淀粉含量的QAc6,在2个不同土壤水分条件下均与环境存在显著互作,对环境互作的贡献率分别为8.99%和47.86%。控制直链淀粉含量的2对上位性QTLs与土壤水分环境显著互作,贡献率较大,分别为32.54%和11.82%。并筛选到5个主效QTL(QGt6b、QGt8、QGt11、QGc1和QPc2)在抗旱育种中可用于蒸煮和营养各品质性状MAS改良。 相似文献
13.
Identification of Genetic Factors Contributing to Heterosis in a Hybrid from Two Elite Maize Inbred Lines Using Molecular Markers 总被引:88,自引:0,他引:88
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The use of molecular markers to identify quantitative trait loci (QTLs) affecting agriculturally important traits has become a key approach in plant genetics-both for understanding the genetic basis of these traits and to help design novel plant improvement programs. In the study reported here, we mapped QTLs (and evaluated their phenotypic effects) associated with seven major traits (including grain yield) in a cross between two widely used elite maize inbred lines, B73 and Mo17, in order to explore two important phenomena in maize genetics-heterosis (hybrid vigor) and genotype-by-environment (G x E) interaction. We also compared two analytical approaches for identifying QTLs, the traditional single-marker method and the more recently described interval-mapping method. Phenotypic evaluations were made on 3168 plots (nearly 100,000 plants) grown in three states. Using 76 markers that represented 90-95% of the maize genome, both analytical methods showed virtually the same results in detecting QTLs affecting grain yield throughout the genome, except on chromosome 6. Fewer QTLs were detected for other quantitative traits measured. Whenever a QTL for grain yield was detected, the heterozygote had a higher phenotype than the respective homozygote (with only one exception) suggesting not only overdominance (or pseudooverdominance) but also that these detected QTLs play a significant role in heterosis. This conclusion was reinforced by a high correlation between grain yield and proportion of heterozygous markers. Although plant materials were grown and measured in six diverse environments (North Carolina, Iowa and Illinois) there was little evidence for G x E interaction for most QTLs. 相似文献
14.
Yun-Feng Xu Si-Shen Li Li-Hui Li Fei-Fei Ma Xiao-Yi Fu Zhan-Liang Shi Hong-Xing Xu Peng-Tao Ma Diao-Guo An 《Molecular breeding : new strategies in plant improvement》2017,37(3):34
The improvement for drought tolerance requires understanding of the genetic control of wheat (Triticum aestivum L.) reaction to drought. In this study, a set of 131 recombinant inbred lines of wheat were investigated under well-watered (WW) and drought stress (DS) environments across 2 years to map quantitative trait loci (QTLs) for yield and physiological traits. A total of 225 QTLs were detected, including 32 non-environment-specific loci that were significant in both DS and WW, one drought-specific locus and two watering-specific loci. Three consistently-expressed QTLs (QTkw-3A.2, QTss-1A, and QScn-7A.1) were identified in at least three environments and the QTkw-1D.1 was significant in DS across the 2 years. By unconditional and conditional QTL analysis, spike number per plant and kernel number per spike were more important than thousand-kernel weight for grain yield (GY) at the given genetic background. Meta-analysis identified 67 meta-QTLs that contained QTLs for at least two traits. High frequency co-location of QTLs was found among either the spike-related traits or the six physiological traits. Four photosynthesis traits (CHL, LWUE, P N, and C i) were co-located with GY and/or yield components on various MQTLs. The results provided QTLs that warrant further study for drought tolerance breeding and are helpful for understanding the genetic basis of drought tolerance and the genetic contribution of yield components to GY at individual QTL level in wheat. 相似文献
15.
Li ZK Yu SB Lafitte HR Huang N Courtois B Hittalmani S Vijayakumar CH Liu GF Wang GC Shashidhar HE Zhuang JY Zheng KL Singh VP Sidhu JS Srivantaneeyakul S Khush GS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,108(1):141-153
One hundred twenty six doubled-haploid (DH) rice lines were evaluated in nine diverse Asian environments to reveal the genetic basis of genotype × environment interactions (GEI) for plant height (PH) and heading date (HD). A subset of lines was also evaluated in four water-limited environments, where the environmental basis of G × E could be more precisely defined. Responses to the environments were resolved into individual QTL × environment interactions using replicated phenotyping and the mixed linear-model approach. A total of 37 main-effect QTLs and 29 epistatic QTLs were identified. On average, these QTLs were detectable in 56% of the environments. When detected in multiple environments, the main effects of most QTLs were consistent in direction but varied considerably in magnitude across environments. Some QTLs had opposite effects in different environments, particularly in water-limited environments, indicating that they responded to the environments differently. Inconsistent QTL detection across environments was due primarily to non- or weak-expression of the QTL, and in part to significant QTL × environment interaction effects in the opposite direction to QTL main effects, and to pronounced epistasis. QTL × environment interactions were trait- and gene-specific. The greater GEI for HD than for PH in rice were reflected by more environment-specific QTLs, greater frequency and magnitude of QTL × environment interaction effects, and more pronounced epistasis for HD than for PH. Our results demonstrated that QTL × environment interaction is an important property of many QTLs, even for highly heritable traits such as height and maturity. Information about QTL × environment interaction is essential if marker-assisted selection is to be applied to the manipulation of quantitative traits.Communicated by G. Wenzel 相似文献
16.
Identification of drought-inducible genes and differentially expressed sequence tags in barley 总被引:9,自引:0,他引:9
Diab AA Teulat-Merah B This D Ozturk NZ Benscher D Sorrells ME 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(7):1417-1425
Drought limits cereal yields in several regions of the world and plant water status plays an important role in tolerance to drought. To investigate and understand the genetic and physiological basis of drought tolerance in barley, differentially expressed sequence tags (dESTs) and candidate genes for the drought response were mapped in a population of 167 F8 recombinant inbred lines derived from a cross between Tadmor (drought tolerant) and Er/Apm (adapted only to specific dry environments). One hundred sequenced probes from two cDNA libraries previously constructed from drought-stressed barley (Hordeum vulgare L., var. Tokak) plants and 12 candidate genes were surveyed for polymorphism, and 33 loci were added to a previously published map. Composite interval mapping was used to identify quantitative trait loci (QTL) associated with drought tolerance including leaf relative water content, leaf osmotic potential, osmotic potential at full turgor, water-soluble carbohydrate concentration, osmotic adjustment, and carbon isotope discrimination. A total of 68 QTLs with a limit of detection score 2.5 were detected for the traits evaluated under two water treatments and the two traits calculated from both treatments. The number of QTLs identified for each trait varied from one to 12, indicating that the genome contains multiple genes affecting different traits. Two candidate genes and ten differentially expressed sequences were associated with QTLs for drought tolerance traits. 相似文献
17.
Baum M Grando S Backes G Jahoor A Sabbagh A Ceccarelli S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(7):1215-1225
A genetic linkage map has been developed for recombinant inbred lines (RILs) of the cross 'Arta' × Hordeum spontaneum 41-1. One hundred and ninety four RILs, randomly chosen from a population of 494 RILs, were mapped with 189 markers including one morphological trait (btr = brittle rachis locus). The linkage map extended to 890 cM. Agronomic traits such as grain yield, biological yield, days to heading, plant height, cold tolerance and others were evaluated at the ICARDA research stations Tel Hadya and Breda during the years 1996–97 and 1997–98. QTLs for agronomic traits related to drought resistance were localized. For the most-important character 'plant height under drought stress', QTLs on 2H, 3H and 7H were detected. The 'plant height' QTLs, specially the one on 3H, showed pleiotropic effects on traits such as days to heading, grain yield and biological yield. QTLs were also identified for other traits associated with adaptation to the Mediterranean environment such as cold tolerance, days to heading and tiller number. The identification of QTLs for agronomic traits is a first step to analyze and to dissect complex characters such as adaptation to drought tolerance.Comunicated by R. Hagemann 相似文献
18.
MacMillan K Emrich K Piepho HP Mullins CE Price AH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(5):953-964
Modifying plant root systems is considered a means of crop improvement targeted to low-resource environments, particularly low nutrient and drought-prone agriculture. The identification of quantitative trait loci (QTLs) for root traits has stimulated marker-assisted breeding to this end, but different QTLs have been detected in different populations of the same species, and importantly, in the same population when grown in different experimental environments. The presence of QTL × environment interaction is implicated, and this must be characterised if the utility of the target QTLs is to be realised. Previous attempts to do this suffer from a lack of control over replicate environments and inadequate statistical rigour. The Bala × Azucena mapping population was grown in two replicate experiments of four treatment environments, a control, a low light, a low soil nitrogen and a low soil water treatment. After a 4 weeks growth, maximum root length, maximum root thickness, root mass below 50 cm, total plant dry mass, % root mass and shoot length were measured. A summary of the overall results is presented in an accompanying paper. Here, QTL analysis by composite interval mapping is presented. A total of 145 QTLs were detected, mapping to 37 discrete loci on all chromosomes. Superficial evidence of QTL × E (great difference in LOD score) was tested by single-marker analysis which confirmed QTL × E for five loci representing only five individual trait-loci interactions. Some loci appeared to be stable across environments. Some QTLs were clearly more or less active under low light, low nitrogen or drought. A few notable loci on chromosomes 1, 2, 3, 5, 7 and 9 are briefly discussed. Also discussed are some remaining statistical shortcomings that will be addressed in another companion paper. 相似文献
19.
玉米雄穗分枝数与主轴长的QTL鉴定 总被引:8,自引:0,他引:8
在包含103个SSR标记的连锁图谱基础上, 运用复合区间作图法检测玉米组合(N87-1×9526 )F3家系在正常与干旱胁迫环境下的雄穗分枝数与主轴长性状QTL。雄穗分枝数在正常环境下被检测到2个QTL座位, 分别位于第5和7连锁群上; 在胁迫环境下被检测到4个QTL座位分别位于 2、5、7和10连锁群上, 其中位于第5和7连锁群上的QTL不仅具有一致性而且与本作图群体中曾检测到的耐旱相关性状QTL存在连锁。雄穗主轴长在正常环境下被检测到2个QTL位于第2和第6连锁群上, 在干旱胁迫环境下被检测到了3个QTL分别于第2、4和10连锁群上, 其中位于第2染色体上的QTL是两种环境下所共同检测到的QTL。分析QTL的遗传作用方式表明, 雄穗分枝数以部分加性效应为主, 而雄主轴长全部表现为显性和超显性。 相似文献
20.
M. J. Asíns P. Mestre J. E. García F. Dicenta E. A. Carbonell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(2-3):358-364
Besides QTL location and the estimation of gene effects, QTL analysis based on genetic markers could be used to comprehensively investigate quantitative trait-related phenomena such as pleiotropy, gene interactions, heterosis, and genotype-by-environment interaction (G x E). Given that the G x E interaction is of great relevance in tree improvement, the objective of the research presented here was to study the effect of years on QTL detection for 15 quantitative traits by means of isozymatic markers in a large progeny group of an intervarietal cross of almond. At least 17 putative QTLs were detected, 3 of which had alleles with opposite effects to those predicted from the parental genotypes. Only 3 QTLs behaved homogeneously over the years. Three possible causes are discussed in relation to this lack of stability: the power of the test statistic being used, the low contribution of the QTL to the genetic variation of the trait, and a differential gene expression dependent on the year (G x E). Most cases showing lack of stability involved traits whose heritability estimates change drastically from year to year and/or whose correlation coefficients between years are low, suggesting the presence of G x E as the most likely cause. A marker-assisted selection scheme to improve late flowering and short flowering duration is suggested for an early and wide screening of the progeny. 相似文献