共查询到20条相似文献,搜索用时 937 毫秒
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Jaydip Mandal Sitakanta Pattnaik Pradeep K. Chand 《In vitro cellular & developmental biology. Plant》2000,36(4):287-292
Summary Propagation and conservation of four pharmaceutically important herbs, Ocimum americanum L. syn. O. canum Sims. (hoary basil); O basilicum L. (swett basil); O. gratissimum L. (shrubby basil); and O. sanctum L. (sacred basil) was attempted using synthetic seed technology. Synthetic seeds were produced by encapsulating axillary
vegetative buds harvested from garden-grown plants of these four Ocimum species in calcium alginate gel. The gel contained Murashige and Skoog (MS) nutrients and 1.1-4.4 μM benzyladenine (BA). Shoots emerged from the encapsulated buds on all six planting media tested. However, the highest frequency
shoot emergence and maximum number of shoots per bud were recorded on media containing BA. Of the six planting media tested,
both shoot and root emergence from the encapsulated buds in a single step was recorded on growth regulator-free MS medium
as well as on vermi-compost moistened with halfstrength MS medium. Rooted shoots were retrieved from the encapsulated buds
of O. americanum, O. basilicum, and O. sanctum on these two media, whereas shoots of O. gratissimum failed to root. The encapsulated buds could be stored for 60 d at 4°C. Plants retrieved from the encapsulated buds were hardened
off and established in soil. 相似文献
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This study demonstrates the importance of preconditioning ofsource tissue in regeneration of multiple shoot buds from severalspecies of Lathyrus. Preconditioned multiple shoots of Lathyruscicera L., L. ochrus (L.) DC. and L. sativus L. were obtainedby germinating seeds on Murashige and Skoog (MS) medium containing50 µM N5-benzylaminopurine (BAP) for 2 to 3 weeks. Multipleshoot bud formation occurred when epicotyl explants of preconditionedshoots were cultured on MS medium containing 550 µMBAP. No shoot regeneration was observed from epicotyl explantswhich were obtained from non-preconditioned shoots. Shoot budswere formed directly on explants without an intervening callusphase after 2 to 3 weeks of culture. Regenerated shoot budsformed healthy shoots which developed prolific and strong rootswhen transferred to MS medium supplemented with 2.5 µMnaphthaleneacetic acid (NAA). Lathyrus cicera L., L. ochrus (L.) DC., Ochrus Vetch, L. sativus L., Lathyrus pea, de novo differentiation, epicotyl, preconditioning with BAP 相似文献
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Dagmar Dykyjová 《Folia Geobotanica》1986,21(1):27-64
Measurements were made, for several seasons, both of the growth rate and biomass production of stands ofBolboschoenus maritimus, the seasonal development of the vertical stand structure together with parallel measurements of microclimatological data including incoming global radiation. Water and bottom soil chemical analyses in relation to the nutrient content in the biomass were compared. The ecological adaptation of the acidophilic subspeciesB. m. ssp.maritimus, growing in south-Bohemian oligotrophic fishpond waters with that of the halophilic ssp.compactus was studied in experimental hydroponic cultures and the results discussed with the findings of other authors from different European habitats. The efficiency of solar energy conversion of incoming radiation was calculated by means of energy content biomass analysis. 相似文献
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B. Czeczuga 《Hydrobiologia》1980,74(1):7-10
The author investigated the presence of various carotenoids in the different parts of the body of Pungitius pungitius (L.) and Gasterosteus aculeatus L. by means of columnar and thin-layer chromatography. The investigations revealed the presence of the following carotenoids: in Pungitius pungitius. α-carotene, β-carotene, β-cryptoxanthin, mutatochrome, zeaxanthin and astaxanthin; in Gasterosteus aculeatus: β-carotene, β-cryptoxanthin, β-carotene epoxide, neothxanthin, canthaxanthin, mutatochrome, lutein, phoenicoxanthin, zeaxanthin, taraxanthin, tunaxanthin, astaxanthin, astaxanthin ester and α-doradexanthin. The total carotenoid content ranged from 2.229 to 138.504 µg/g wet weight. 相似文献
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Abstract The various stages of female gametophyte development and embryogenesis in S. spiralis and S. aestivalis are described. In both species the reproductive cycle is sexual. Some peculiarities are present: the female gametophyte is usually 6-7-8-nucleate; after double fertilization a single endospermatic cell is formed; the proembryo appears differentiated and is made up of different cells in the chalazal and micropylar ends; a single basal cell in the proembryo acts as suspensor. 相似文献
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KARIN M. VALANT-VETSCHERA 《Botanical journal of the Linnean Society. Linnean Society of London》1996,121(2):159-168
The lectotypes of Achillea chillea santolina L. and A. falcata L. (Achillea sect. Achillea) have been confrmed to be in the Clifford Herbarium. A. santolina L. cannot be retained as the generitype, and the selection of A. millefolium L. instead is confirmed. The species concept of the Linnaean A. santolina did not correspond to the post-Linnaean usage of this name. In most cases, A. santolina L. had been confused with A. wilhelmii C. Koch, but morphological differences separate those two species distinctly. The only extant element of A. falcata L. is found in the Clifford Herbarium, and this specimen corresponds principally to what is considered to be A. falcata today. Deviation in leaf structures is due to this material being of cultivated origin. 相似文献
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Okoli B. E. etNyananyo B. L. (1988): Palynology ofTelfairia L. (Cucurbitaceae).—Folia Geobot. Phytotax., Praha, 23: 281–283.—Scanning and transmission electron microscopic studies were carried out on the pollen of the two species ofTelfairia, T. pedata (Sims.) Hooker andT. occidentolis Hooker fil. Pollen grains in both species are spheroidal, tricolporate and tectate. The extexine is finely reticulate. The tectum, foot layer and columellae are all well developed. Significant differences of taxonomic value do not exist in the structure of the pollen of the two species. 相似文献
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Kyria Cilene de A. Bortoleti Ana Maria Benko-Iseppon Natoniel Franklin de Melo Ana Christina Brasileiro-Vidal 《Plant Systematics and Evolution》2012,298(3):689-693
A comparative chromosomal evaluation was carried out between Vigna unguiculata (cowpea) and V. radiata (mung bean) with chromomycin A3 (CMA3)/4’,6-diamidino-2-phenylindole (DAPI) banding and fluorescent in situ hybridization (FISH) using 5S/45S ribosomal DNA (rDNA)
probes. Both species had symmetric karyotypes (2n = 22), with prevalence of centromeres in chromosomes at median (m) and submedian (sm) regions and chromosomes ranging in
size from 2.1 to 1.25 μm (V. unguiculata) and 2.18 to 0.93 μm (V. radiata). Three different banding patterns were identified for V. unguiculata: CMA3+/DAPI0, CMA3++/DAPI–, and CMA3+/DAPI–. The CMA3+/DAPI0 bands were observed in the pericentromeric regions of all chromosomes, while the CMA3++/DAPI– and CMA3+/DAPI– bands were co-localized with the 45S rDNA in the subtelomeric position (chromosomes B, G, and D, J, respectively) and in
the proximal position in chromosome F. Two pairs of chromosomes (D and I) bearing interstitial 5S rDNA have been also identified.
Vigna radiata displayed CMA30/DAPI+ bands distributed in the centromeric region of chromosomes B, C, and F, while CMA3++/DAPI– bands were co-localized with the 45S rDNA sites in the subtelomeric position of the short arm in the F and K chromosome pairs.
Three pairs of 5S rDNA sites were identified, the first in the proximal region of the long arm in chromosome E and the two
others in the proximal and subterminal positions in the long arm of chromosome J. These data highlight some divergences regarding
the amount and composition of the heterochromatin in both species, allowing the identification of individual chromosomes in
V. unguiculata and V. radiata, and a comparison with other members of the Phaseoloid clade. 相似文献
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Lennart Lundgren 《Zoologica scripta》1975,4(1):253-258
In several growers' reports Solatium lycopersicum, Sambucus nigra. Thymus vulgaris, Salvia officinalis, Artemisia absinthium, A. abrotanum , and Allium cepa are said to decrease the oviposition of Pieris brassicae, P. rapae and P. napi. In the present study the butterflies were fed with honey automats and reared throughout the year in artificial light in an insec-tarium. In a dual-choice chamber with a slow throughflow of air two equally sized cabbage leaves were placed on opposite sides. Significantly fewer eggs were layed on the cabbage leaf on which extracts of the mentioned plants had been applied. Ten butterflies were used in each experiment. The chemoreceptors and the chemicals involved are not identified but the inhibitory substances are surely secondary plant substances. Acceptance or rejection of secondary plant metabolics determines the complicated food relationships between plants and insects. The use of secondary plant substances for ecological control of insect pests is proposed. 相似文献
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