共查询到20条相似文献,搜索用时 31 毫秒
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Differential role of two VDR coactivators, DRIP205 and SRC-3, in keratinocyte proliferation and differentiation 总被引:3,自引:0,他引:3
Oda Y Ishikawa MH Hawker NP Yun QC Bikle DD 《The Journal of steroid biochemistry and molecular biology》2007,103(3-5):776-780
Cell programs such as proliferation and differentiation involve the selective activation and repression of gene expression. The vitamin D receptor (VDR), through 1,25(OH)(2)D(3), controls the proliferation and differentiation of keratinocytes. Previously, we have identified two VDR binding coactivator complexes. In proliferating keratinocytes VDR bound preferentially to the DRIP complex, whereas in differentiated keratinocytes the SRC complex was preferred. We proposed that different coactivators are required for sequential gene regulation in the transition from proliferation to differentiation. Here we examined the roles of DRIP205 and SRC-3 in this transition. Silencing of DRIP205 and VDR caused hyperproliferation of keratinocytes, demonstrated by increased XTT and BrdU incorporation. SRC-3 silencing, on the other hand, did not have an effect on proliferation. In contrast, SRC-3 as well as DRIP205 and VDR silencing blocked keratinocyte differentiation as shown by decreased expression of keratin 1 and filaggrin. These results are consistent with the differential localization of DRIP205 and SRC-3 in skin. These results indicate that DRIP205 is required for keratinocyte proliferation. Both DRIP205 and SRC-3 are required for the keratinocyte differentiation. These results support the concept that the selective use of coactivators by VDR underlies the selective regulation of gene expression in keratinocyte proliferation and differentiation. 相似文献
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Regulation of the bone-specific osteocalcin gene by p300 requires Runx2/Cbfa1 and the vitamin D3 receptor but not p300 intrinsic histone acetyltransferase activity 下载免费PDF全文
Sierra J Villagra A Paredes R Cruzat F Gutierrez S Javed A Arriagada G Olate J Imschenetzky M Van Wijnen AJ Lian JB Stein GS Stein JL Montecino M 《Molecular and cellular biology》2003,23(9):3339-3351
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Bone-specific transcription factor Runx2 interacts with the 1alpha,25-dihydroxyvitamin D3 receptor to up-regulate rat osteocalcin gene expression in osteoblastic cells 总被引:3,自引:0,他引:3 下载免费PDF全文
Paredes R Arriagada G Cruzat F Villagra A Olate J Zaidi K van Wijnen A Lian JB Stein GS Stein JL Montecino M 《Molecular and cellular biology》2004,24(20):8847-8861
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Bravo S Paredes R Izaurieta P Lian JB Stein JL Stein GS Hinrichs MV Olate J Aguayo LG Montecino M 《Journal of cellular biochemistry》2006,99(4):995-1000
1alpha,25-dihydroxy vitamin D3 has a major role in the regulation of the bone metabolism as it promotes the expression of key bone-related proteins in osteoblastic cells. In recent years it has become increasingly evident that in addition to its well-established genomic actions, 1alpha,25-dihydroxy vitamin D3 induces non-genomic responses by acting through a specific plasma membrane-associated receptor. Results from several groups suggest that the classical nuclear 1alpha,25-dihydroxy vitamin D3 receptor (VDR) is also responsible for these non-genomic actions of 1alpha,25-dihydroxy vitamin D3. Here, we have used siRNA to suppress the expression of VDR in osteoblastic cells and assessed the role of VDR in the non-genomic response to 1alpha,25-dihydroxy vitamin D3. We report that expression of the classic VDR in osteoblasts is required to generate a rapid 1alpha,25-dihydroxy vitamin D3-mediated increase in the intracellular Ca(2+) concentration, a hallmark of the non-genomic actions of 1alpha,25-dihydroxy vitamin D3 in these cells. 相似文献
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