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1.
Guo Yang Jinfeng Lü Jaime A. Teixeira da Silva Hongfeng Chen Guohua Ma 《In vitro cellular & developmental biology. Plant》2014,50(4):451-457
Dayaoshania cotinifolia W. T. Wang is a rare and endangered member of the Gesneriaceae family which is endemic to China. To conserve this species, an efficient in vitro propagation and regeneration system via shoot organogenesis was established from young leaf explants. Adventitious shoot induction was possible within 50–60 d on basal Murashige and Skoog medium supplemented with 1–3 μM 6-benzyladenine, although 5 μM 6-benzyladenine induced hyperhydricity. Basal medium containing 1–5 μM thidiazuron induced fewer shoots, while 1–5 μM α-naphthaleneacetic acid induced numerous adventitious roots and a few adventitious shoots. However, when thidiazuron and α-naphthaleneacetic acid were combined, both the induction percentage and number of shoots increased. Leaf explants cultured on induction medium supplemented with 1–5 μM 2,4-dichlorophenoxyacetic acid become necrotic and died. Induction medium supplemented with 1 μM α-naphthaleneacetic acid and 1–3 μM 6-benzyladenine was optimal for inducing adventitious shoots as was the combination of 1–3 μM thidiazuron and 1 μM α-naphthaleneacetic acid. Induction medium containing 2.0 μM 6-benzyladenine and 0.5 μM indole-3-acetic acid was optimal for the multiplication of adventitious shoots. Rooting was achieved on half-strength MS medium supplemented with 3.0 μM indole-3-acetic acid or α-naphthaleneacetic acid and 0.1% activated charcoal. Plantlets were transplanted to a mixture of sand, vermiculite, and humus (1:1:1); 92% survived. This protocol is a unique and effective means to micropropagate this rare and important plant and could serve as a solution for in vitro and ex vitro conservation. 相似文献
2.
Pereira Ana Maria S. Bertoni Bianca W. Appezzato-da-Glória Beatriz Araujo Alba R. B. Januário Ana Helena Lourenço Miriam V. França Suzelei C. 《Plant Cell, Tissue and Organ Culture》2000,60(1):47-53
The establishment of a micropropagation protocol for Pothomorphe umbellata was carried out using leaf segments cultured on 1/4 strength Murashige and Skoog medium supplemented with 0.5 mg l-1 6-benzyladenine, 0.1 mg l-1 gibberelic acid added with 10 g l-1 sucrose. Rooting was achieved using MS medium devoid of growth regulators. An anatomical study confirmed shoot regeneration via direct organogenesis. 相似文献
3.
Mingjia Yang Xiangming Xie Xiaoqing He Fangqiu Zhang 《Plant Cell, Tissue and Organ Culture》2006,85(2):241-245
In this paper we report the establishment of Acacia crassicarpa regeneration through organogenesis. We used phyllode (leaf) explants excised from 60-day-old in vitro seedlings for green compact nodule induction and, tested Murashige and Skoog (MS) media supplemented with various concentrations of 1-phenyl-3-(thiadiazol-5-yl) urea (thidiazuron) (TDZ) and α-naphthaleneacetic acid (NAA). Under the optimized condition, green compact nodules and adventitious shoots were induced in 10 and 40 days, respectively, on the medium containing a combination of 0.5 mg l−1 TDZ and 0.5 mg l−1 NAA. This medium also yielded the highest rate (56%) of adventitious shoots forming from the nodules. Efficient shoot elongation was achieved by transferring the clusters of adventitious shoots to medium containing 0.1 mg l−1 TDZ within 2 months. The elongated adventitious shoots were rooted at a rate of 96.5% on half-strength MS medium with 0.5 mg l−1 3-indolebutyric acid (IBA) in 1 month. Rooted plantlets were hardened and successfully established in soil with an 80% survival rate. To our knowledge, this is the first report describing a detailed protocol for regeneration through organogenesis using phyllodes as explants for A. crassicarpa. 相似文献
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Plant regeneration from seedling explants of green bean (Phaseolus vulgaris L) via organogenesis 总被引:1,自引:0,他引:1
Chandra I. Franklin Tony N. Trieu Robert A. Gonzales Richard A. Dixon 《Plant Cell, Tissue and Organ Culture》1991,24(3):199-206
Green bean (Phaseolus vulgaris L.) plants were regenerated from 3-day old seedling explants via organogenesis. The explants contained a cotyledon and a small portion (2–3 mm) of embryonic axis split in half. Explants were cultured on a defined medium containing glutamine as the sole nitrogen source. A ring of meristematic tissue was produced at the base of the axillary bud located at the cotyledonary node. The meristematic tissue was produced only if the axillary bud was present together with the cotyledon in the explant. Buds and shoots developed from the meristematic ring. Selected shoots produced roots when excised from the cluster of buds and transferred to root induction medium. Rooted shoots (plantlets) grew well and produced viable seeds when grown in the greenhouse. Histological studies revealed the origin of buds from the peripheral layers of the meristematic ring.Production of buds and shoots was a continuous process, so that new shoots could be removed from the explant for plantlet production every 10–14 days. With the cultivar Dark Red Kidney, an average of 49 buds and 8 shoots were regenerated per explant by 30 days after culture initiation. Sixty-seven percent of the shoots produced roots, and 90–95% of the plantlets survived greenhouse acclimatization to produce healthy plants. 相似文献
6.
Plant regeneration from leaf explants of Rhodiola fastigiata 总被引:1,自引:0,他引:1
Hai-Jun Liu Yan Xu Yu-Jun Liu Chun-Zhao Liu 《In vitro cellular & developmental biology. Plant》2006,42(4):345-347
Summary An efficient plant regeneration protocol for rapidly propagating Rhodiola fastigiata (Hk. f. et Thoms.) S.H.FU, a traditional Chinese medicinal plant, was developed. Shoot organogenesis occurred from the leaf
explants inoculated on medium with appropriate supplements of plant growth regulators. Up to 5.3 shoots formed per leaf explant
cultured on a medium containing 13.32 μM 6-benzylaminopurine (BA) and 0.54 μM 1-naphthaleneacetic acid (NAA). Regenerated shoots formed complete plantlets on a medium containing 1.48 μM indole-3-butyric acid (IBA), and mature plants were established, acclimatized, and thrived in greenhouse conditions. The
regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of
medicinally active constituents of the elite Chinese medicinal plant. 相似文献
7.
J. Jerome Jeyakumar M. Kamaraj M. Thiruvengadam 《Journal of plant biochemistry and biotechnology.》2014,23(3):307-315
An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature petiole explants of West Indian gherkin (Cucumis anguria L.). Calluses were induced from immature petiole explants excised on 7-day-old in vitro seedlings and mature petiole explants of 40-day-old in vivo plants. The maximum frequency of immature petiole explants (98.0 %) and mature petiole (91.5 %) produced green, compact organogenic callus in Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g l?1 sucrose, 8.0 g l?1 agar and 4.0 μM naphthalene acetic acid (NAA) with 2.0 μM benzyl amino purine (BAP) after two successive subculture at 11 days interval. Adventitious shoots were produced from the organogenic callus when it was transferred to MSB5 medium supplemented with 3.0 μM TDZ, 1.0 μM NAA and 0.05 mM L-glutamine with shoot induction frequency of immature petiole 45 shoots and mature petiole 40 shoots per explant. The shoots were excised from callus and elongated in MSB5 medium fortified with 3.0 μM gibberellic acid (GA3). Then elongated shoots were rooted in half strength MSB5 medium supplemented with 3.0 μM indole 3-butyric acid (IBA). Histological analyses of the regeneration process confirmed the indirect organogenesis pattern. Plantlets with well-developed shoot and root systems were successfully acclimatized (95 %) in winter season and exhibited normal morphology and growth characteristics. The survival percentage differed with seasonal variations. 相似文献
8.
Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the same G. clandestina accession. We were not successful in regenerating plants from root tissue of G. clandestina.Abbreviations 6-BA
6-Benzyladenine
- FAA
Formalin
- NAA
Naphthalenacetic acid
- IAA
Indoleacetic acid
- Na2EDTA
Disodium salt Ethylenediamine tetraacetic acid
- Fe-NaEDTA
Ferric-Sodium salt Ethylenediamine tetraacetic acid 相似文献
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Production of Agrobacterium tumefaciens-mediated transgenic plants, via direct shoot bud organogenesis from leaves of Catharanthus roseus, is reported. A. tumefaciens harbouring the plasmid pBI121 with GUS gene uidA and kanamycin resistance gene nptII was used. Highest transformation efficiency of 1.4 transgenic shoots/responded explant was obtained when pre-plasmolysed
leaves, pre-incubated on shoot bud induction medium for 10 days, were subjected to sonication for 30 s prior to transformation.
Using a selection medium containing 50 mg kanamycin l−1, transformants grew into micro-shoots and formed roots on a hormone-free half strength MS medium. The transgenic nature of
the regenerated plants was confirmed by PCR amplification of uidA gene and GUS histochemical assay. 相似文献
14.
J. Y. Choi H. J. Kim C. H. Lee J. M. Bae Y. S. Chung J. S. Shin N. I. Hyung 《In vitro cellular & developmental biology. Plant》2001,37(2):274-279
Summary An efficient and simple plant regeneration system via organogenesis from leaf segments of persimmon (Diospyros kaki Thunb.) cultivars ‘Fuyu’ and ‘Nishimurawase’ has been developed. The regeneration capacity was influenced by the culture
vessels, gelling agents, plant growth regulators, and light conditions. Leaf explants taken from in vitro shoots were cultured on a modified Murashige and Skoog medium (MS1/2N), for 16 wk without transfer to fresh medium. Adventious
shoots appeared after 4 and 8 wk in culture of ‘Nishimurawase’ and ‘Fuyu’ tissues, respectively. The culture of leaf explants
in Erlenmeyer flasks with medium containing 4 g l−1 agar enhanced shoot formation in comparison to media with increased agar concentrations. Optimal shoot regeneration was obtained
with 5 mg l−1 (22.8 μM) zeatin and 0.1 mg l−1 (0.05 μM) indole-3-butyric acid (IBA) for ‘Nishimurawase’, and 10 mg l−1 (45.6 μM) zeatin and 0.1 mg l−1 (0.05 μM) IBA for ‘Fuyn’. Shoot regeneration frequencies in both cultivars were 100%, and shoot numbers per explant reached up to
9.2 for ‘Nishimurawase’ and 2.2 for ‘Fuyu’. Dark incubation during the first 4–5 wk was the most effective condition to successfully
influence shoot regeneration in both cultivars. While dark incubation was essential for adventitious shoot formation by ‘Fuyu’,
it was only slightly beneficial to ‘Nishimurawase’. More than 80% of the regenerated shoots rooted within 4 wk on hormone-free
MS1/2N demium after having been dipped for 30 s in 250 mg l−1 (1.1. mM) IBA solution. 相似文献
15.
An efficient protocol has been developed for direct shoot organogenesis from embryo axes derived from mature seeds of two different landraces of Bambara groundnut. Multiple shoots were initiated on several media containing different concentrations and combinations of benzylaminopurine (BAP) or thidiazuron (TDZ). Efficient regeneration occurred when the embryo axes were first plated for 6 days on a medium containing high concentrations of BAP (1 mg/l) and alpha-naphthaleneacetic acid (NAA, 1 mg/l) and then cut transversely and transferred onto a medium containing 1.5 mg/l BAP. Shoot regeneration frequency was 100% and from five to eight shoots per explant were obtained. The importance of using embryo explants and cytokinins in the culture media, with respect to controlling the development of a highly organogenic system, was demonstrated. Histological studies revealed that proliferating buds originated directly from the superficial layers of the explants without an intermediate callus phase. The regenerated shoots were rooted on a medium containing 1 mg/l NAA and then transferred to the greenhouse. Flow cytometric analyses and chloroplast counts of guard cells suggested that the regenerants were diploid. All were morphologically normal and fertile. The short duration, high efficiency and low frequency of somaclonal variation of this system make it well suited for wider biotechnological applications of Bambara groundnut-a neglected and under-utilized crop. 相似文献
16.
C. K. Singh Sandeep R. Raj V. R. Patil P. S. Jaiswal N. Subhash 《In vitro cellular & developmental biology. Plant》2013,49(2):216-222
Sandalwood (Santalum album L.) is a small evergreen, hemi-parasitic tree having more than 18 woody species that are mostly distributed in South Asia, Australia, and Hawaii. Its economical importance is derived from its heartwood oil, but its difficult propagation makes conservation essential. The percentage of seed germination is poor and germination time exceeds 12 mo. Vegetative propagation can be accomplished by grafting, air layering, or with root suckers, but the production of clones is inefficient and time consuming. In this study, efficient plant regeneration was achieved via indirect organogenesis from callus cultures derived from leaf tissues of S. album. Callus induction was induced when leaf explants were cultured on woody plant media (WPM) supplemented with either thidiazuron (TDZ) or 2,4-dichlorophenoxyacetic acid. The highest callus frequency (100%) was obtained when leaf tissue was cultured in the medium with 0.4 mg?l?1 TDZ. Fresh weight (141.92 mg) and dry weight (47 mg) of leaf-derived callus were highest in the medium supplemented with 0.8 mg?l?1 TDZ. The WPM medium supplemented with 2.5 mg?l?1 BA?+?0.4 mg?l?1 NAA was the most effective, producing the highest number of shoot buds (24.6) per callus. The highest number of shoots per explant (20.67) and shoot length (5.17 cm) were observed in media supplemented with 5.0 mg?l?1 BA and 3.0 mg?1?1 Kn, respectively. Plantlets were rooted on WPM medium with different concentrations of indole-3-butyric acid (IBA). The highest rooting percentage (91.67) and survival were achieved using WPM media with 1.5 mg?l?1 IBA. All plantlets survived acclimatization, producing healthy plants in the greenhouse. The current investigation showed efficient in vitro regeneration capabilities of S. album from leaf explants. 相似文献
17.
Winthrop B. Phippen James E. Simon 《In vitro cellular & developmental biology. Plant》2000,36(4):250-254
Summary An efficient plant regeneration protocol was successfully developed for basil (Ocimum basilicum L.). Explants from 1 mo. old seedlings yielded the highest frequency of 85% regeneration with an average of 5.1 shoots per
explant. The regeneration protocol was performed on three basil varieties (Sweet Dani; methylcinnamate; Green Purple Ruffles).
Callus and shoot induction was initiated on Murashige and Skoog basal medium supplemented with thidiazuron (16.8 μM) for approximately 30 d. Shoot induction and development were achieved by refreshing the induction medium after 14 d. The
most morphogenetically responsive explants were from the first fully expanded true leaves of greenhouse-grown basil seedlings.
All developing bud tissue demonstrated temporary anthocyanin expression; however, anthocyanin expression in Green Purple Ruffles
remained stable until maturity. Developing shoots were rooted in the dark on media with thidiazuron removed. Within 20 d,
rooted plantlets were transferred and acclimatized under greenhouse conditions where they developed normal morphological characteristics.
This is the first report of a successful in vitro regeneration system for basil through primary callus. 相似文献
18.
Phillip J. Ainsley Graham G. Collins Margaret Sedgley 《In vitro cellular & developmental biology. Plant》2000,36(6):470-474
Summary A method has been developed to facilitate shoot formation from leaf explants of almond. Leaves were dissected from micropropagated
shoot cultures of the commercial cultivars Nonpareil and Ne Plus Ultra, and sections incubated on Almehdi and Parfitt's (1986)
basal medium (AP) with varied plant growth-regulator conditions. Three auxins, 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic
acid (NAA), and indole-3-butyric acid (IBA), in combination with two cytokinins, benzylaminopurine (BA) and thidiazuron (TDZ),
were tested at various concentrations along with casein hydrolysate (CH) to determine, the conditions most conducive to adventitious
shoot regeneration. Response to the tested plant growth-regulator conditions varied with genotype. Of the three auxins tested,
NAA and IBA induced adventitious shoots from Ne Plus Ultra explants, but only IBA was effective for Nonpareil. For the cytokinins,
shoot development from Ne Plus Ultra occurred in the presence of either BA or TDZ, whereas for Nonpareil only TDZ was effective
unless CH was incorporated in the basal medium. The inclusion of CH (0.1% w/v) improved callus morphology, and increased regeneration
frequencies for both cultivars. Maximum regeneration frequencies for Ne Plus Ultra (44.4%) and Nonpareil (5.5%) were achieved
on AP basal salts supplemented with CH, IBA (9.8 μM), and TDZ at 22.7 and 6.8 μM, respectively. 相似文献
19.
Andrzej Borychowski Katarzyna Niemirowicz-Szczytt Magdalena Jędraszko 《Acta Physiologiae Plantarum》2002,24(3):257-264
Morphogenetic potential of hypocotyl and cotyledon explants of the three Polish Capsicum annuum L. cultivars (Kujawianka, Passat and Zorza) was studied to develop a reliable plant regeneration protocol. Out of 8 and 15
combinations of growth regulators used in the first and second series respectively, the best medium contained BAP (5 mg·l−1) and IAA (1 mg·l−1). Media containing thidiazuron (TDZ) and IAA proved to be worse than those with BAP and IAA. Additionally, it was indicated
that hypocotyl explants placed upside-down developed more adventious buds. ‘Passat’ was the most responsive variety (approximately
40 % of both types of explants produced buds).
In the second part of experiment the aim was to stimulate shoot induction in the conditions most adapted to Agrobacterium transformation. ‘Bryza’ replaced cv ‘Kujawianka’ and proved to be better than ‘Passat’, previously distinguished as a highly
responsive cultivar. The experiments confirmed a significant effect of the hypocotyl explant length and induction period on
shoot regeneration. Finally, the optimum concentration of carbenicillin and kanamycin was determined. 相似文献
20.
Q. L. Xu Y. H. Xie H. Ru X. Hu Ch. Y. Wang X. Yu. Wang 《Russian Journal of Plant Physiology》2009,56(4):546-550
An efficient system for in vitro regeneration of red leaf beet, a variety of leaf beet (Beta vulgaris L. var cicla L.) generally used to decorate parterre and to prepare betacyanin, was developed for the first time in the present study.
Shoot tip and petiole explants from the sterile seedlings, precultured on Murashige and Skoog (MS) medium with 15 mg/l 6-benzyladenine
(BA) and 3% sucrose at 16 °C for 30 days, could form 81.02 and 17.33% translucent nodular (TN) calli, respectively. All TN
calli were able to differentiate into adventitious shoots under the same culture conditions. Each explant with TN callus from
the shoot tip and petiole could generate 8.65 shoots on average. It was found that both preculture of sterile seedlings and
culture of explants at low temperature (16 °C) were vital for TN callus induction and adventitious bud formation of red leaf
beet. The best condition for rooting was 0.5-strength MS medium with 10 g/l sucrose. After being transplanted into soil, plantlets
grew well and could flower and bear fruits. Histological observation revealed that TN callus was derived from the cells of
vascular tissue of the petiole and that adventitious shoots were formed through organogenesis. The factors influencing in
vitro micropropagation are also discussed.
Published in Russian in Fiziologiya Rastenii, 2009, Vol. 56, No. 4, pp. 603–608.
This text was submitted by the autors in English. 相似文献