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Changes in seeds of soybeans (Glycine max [L.] Merr. var. Wayne) which occur during accelerated aging (41 C, 100% relative humidity) showed subsequent loss of vigor, a decline in early respiratory activity, increased leakage of electrolytes, losses of as much as 10% dry weight from imbibing cotyledons, and a decrease in the swelling response of the imbibing system (seed plus H2O). Each of these changes with aging is interpreted as resulting from deteriorative changes in membranes. 相似文献
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Lipid changes during natural aging of soybean seeds 总被引:1,自引:0,他引:1
When soybean seeds [ Glycine max (L.) Merr. cv. Wayne] were stored at approximately 4°C and low humidity for 44 months ("natural aging") there was a marked decline in vigor and viability which was associated with a decrease in the proportion of the polyunsaturated fatty acids. Other lipid parameters showed little change. Seeds subjected to high humidity at 40°C for several days ("accelerated aging") experience a comparable decline in vigor and viability, but without the change in fatty acid polyunsaturation. These results indicate that accelerated aging may cause loss of vigor in a manner quite different from natural aging. The accelerated aging treatment is therefore of limited usefulness in investigations of the mechanism of natural seed aging. 相似文献
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Oxygen-dependent antagonism of lipid peroxidation 总被引:4,自引:0,他引:4
Measurements of the rates for formation of conjugated dienes, malonylaldehyde, and lipid hydroperoxides show that increasing the concentration of O2 from 0.11 mM to 0.35 mM or 0.69 mM can slow the rate of linoleic acid peroxidation in a xanthine oxidase/hypoxanthine system. This effect is seen at pH 7.0 but not 7.4 and depends on the presence of monounsaturated fatty acids (oleic, cis, or trans vaccenic acid). Oxygen antagonism of ascorbic acid-iron-EDTA mediated lipid peroxidation is similarly dependent on fatty acid mixtures and occurs at pH 5.0 and 6.0 but not 7.0. The efficiency of initiation of peroxidation in the xanthine oxidase system is unaffected by monounsaturated fatty acids and O2 concentration. Increasing the O2 concentration increases the rate of superoxide radical production, but there is no change in salicylate hydroxylation (e.g., OH. production) or ferrous ion concentration. Oxygen-mediated slower rates of lipid peroxidation are associated with either increased H2O2 production or, based on an indirect assay, singlet O2 production. Increased O2 concentrations increase the rate of azobisisobutyronitrile-initiated lipid peroxidation as expected but addition of exogenous superoxide radicals slows the rate. Under similar conditions superoxide reacts with fatty acids to produce singlet O2. Overall, the data suggest that O2-mediated antagonism occurs because of termination reactions between hydroperoxyl (HO2.) and organic radicals, and singlet O2 or H2O2 are products of these reactions. 相似文献
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Oxygen-dependent anti-Salmonella activity of macrophages 总被引:9,自引:0,他引:9
Numerous observations have established a crucial role for phagocytic cells in host resistance to Salmonella. Activated macrophages rely on a complex array of oxygen-dependent antimicrobial molecules to inhibit or kill intracellular Salmonella. An initial oxidative bactericidal phase, which is dependent on the respiratory burst phagocyte oxidase (phox) is succeeded by a prolonged nitrosative bacteriostatic phase, which is dependent on inducible nitric oxide synthase (iNOS). The sequential contribution of phox and iNOS to anti-Salmonella innate immunity has been demonstrated both in vitro and in vivo. The temporal progression from the predominant production of reactive oxygen species to the production of nitrogen oxides could optimize the initial reduction in microbial burden while minimizing the immunopathological consequences of the host inflammatory response. 相似文献
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Accelerated aging predisposed seeds to imbibition injury. Slowing the rate of hydration prevented the loss of germinability due to imbibition injury. Germinability of accelerated aged seeds (50 hours) was increased from 10 to 90% by controlling the rate of imbibition. Slow hydration also prevented seed electrolyte leakage. This may indicate that cell membrane permeability or rupture was a major factor contributing to the loss of germinability after aging.
Reversal of the effects of aging (repair) was accomplished by slowly inbibing and then redrying seeds (priming). This treatment lowered steep water conductivity by a factor of 2 to 5. Priming also increased the per cent germination of low vigor seeds. The mechanism of this reversal was probably metabolic because it depended on temperature, seed moisture, and treatment duration.
Priming doubled the survival of seeds in the accelerated aging vigor test. The `rejuvenation' was accepted as evidence for metabolic repair. Since the `vigor' of seeds was increased by priming, metabolic repair probably included other subcellular components as well as the plasma membrane.
相似文献7.
Oxygen-dependent gene expression in fishes 总被引:2,自引:0,他引:2
Nikinmaa M Rees BB 《American journal of physiology. Regulatory, integrative and comparative physiology》2005,288(5):R1079-R1090
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J. M. Sung 《Physiologia plantarum》1996,97(1):85-89
The possible role of lipid peroxidation in seed deterioration was investigated during natural aging and accelerated aging of seeds of edible soybean ( Glycine max [L], Merr. cv. Kaohsiung Selection No. 1). Natural aging was achieved by sealing the seeds in aluminum foil bags coated with polyethylene and storing the seeds at room temperature for 3 to 12 months. Accelerated aging was obtained by incubating the seeds at 45°C and close to 100% relative humidity for 3 to 12 days, after which the seeds were air dried to their original moisture level (8%). The results indicate that both natural and accelerated aging enhanced lipid peroxidation, as germination was depressed. Aging also inhibited the activity of superoxide dismutase, catalase, ascorbate peroxidase and peroxidase. The changes in germination and physiological activities, expressed as a function of aging duration, were somewhat similar in the two aging treatments. 相似文献
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P Modenesi 《Bollettino della Società italiana di biologia sperimentale》1979,55(23):2395-2400
Alcohol dehydrogenase activity has been demonstrated in section of fresh and aged pea seeds, both air-dried and during germination. The activity in the various tissues has been observed using nitro-blue tetrazolium. From the comparison a general decrease appear in aged pea seeds and a tendency to a shift in the peaks of alcohol deydrogenase activity in the considered tissues (epidermis upper and lower, hypodermis, storage parenchyma and vascular system). The procambial zone forms the tracheary elements during germination, but in aged seeds this occur only rarely. The experiments here reported confirm the hypothesis that seed ageing includes changes in enzyme activity and in morphogenetic pathways. 相似文献
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Oxygen-dependent xylitol metabolism in Pichia stipitis 总被引:1,自引:0,他引:1
Pichia stipitis CBS 6054 was cultivated in chemostat cultures under aerobic and oxygen-limited conditions with xylitol alone, a mixture of
xylitol and glucose and a mixture of xylitol and xylose. Xylitol metabolism was strictly respiratory and no ethanol was formed.
Simultaneous feeding of xylitol and glucose and xylitol and xylose to oxygen-limited xylitol-pregrown cells resulted in ethanol
formation. In vitro both pyruvate decarboxylase activity and alcohol dehydrogenase activity were present in cells metabolising
xylitol under oxygen-limited conditions; however, this did not result in ethanol formation. Glucose, xylose and xylitol utilisation,
respectively, were compared under anaerobic conditions with regard to growth rate, carbon source and oxygenation level during
pre-cultivation. Irrespective of pre-growth conditions, xylitol was not metabolised under anaerobic conditions, whereas ethanol
was formed from both xylose and glucose. Anaerobic xylose utilisation required induction of a xylose-utilising metabolic pathway
during pre-cultivation.
Received: 23 February 1999 / Received last revision: 20 July 1999 / Accepted: 1 August 1999 相似文献
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The chemical reactions that may lead to the loss of seed viability were investigated both during the accelerated aging and natural aging of soybeans ( Glycine max Merrill cv. Chippewa 64). Under conditions of accelerated aging (36°C and 75% RH), fluorescence of soluble proteins accumulated, which was closely correlated with the loss of seed germinability and vigor. We were able to show this correlation by using partially purified proteins for the assay. Fluorescence also increased in seeds under good storage conditions (5°C for up to 21 years), although there was a less significant correlation between seed viability and the accumulation of fluorescent products during the time of natural aging. The rise in protein fluorescence is interpreted as an increase of Maillard products. The carbonyl content of soluble proteins (a measure of the oxidative damage) did not change significantly during either accelerated aging or natural aging: however the elimination of carbonyls during germination seemed to be hindered in seeds that had poor germination. The Maillard reaction may be a consequence of the formation of reducing sugars through a gradual hydrolysis of oligosaccharides during aging. Preliminary evidence from the natural aging study showed that, when seeds were in the glassy state, the sugar hydrolysis was inhibited. These results suggest that the Maillard reaction and oxidative reaction may play an important role in seed deterioration. 相似文献
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Luminol-dependent chemiluminescence was used as a monitor of reactive oxidant generation during phagocytosis of Staphylococcus aureus by human neutrophils. Reactive oxidants play a crucial role in the killing of this organism because: (a) S. aureus was killed most rapidly when the rate of increase of chemiluminescence was greatest; (b) neutrophils which had been activated to generate reactive oxidants by re-aeration of anaerobic suspensions killed this bacterium more efficiently than control suspensions; and (c) neutrophils from a patient with chronic granulomatous disease could neither generate reactive oxidants nor kill S. aureus. 相似文献
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Dan Xuming Wang Chengran Su Yanning Zhang Ailing Wang Ruijia Khan Imran Huang Linkai 《Molecular biology reports》2020,47(8):5747-5754
Molecular Biology Reports - Seed is an important way to store germplasm resources but its genetic integrity will decrease during long-term preservation. So, it’s essential to update seeds... 相似文献
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Oxygen-dependent regulation of mitochondrial respiration by hypoxia-inducible factor 1 总被引:1,自引:0,他引:1
Semenza GL 《The Biochemical journal》2007,405(1):1-9
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Villiers TA 《Plant physiology》1974,53(6):875-878
Increase in moisture content of seeds of Lactuca sativa L. and Fraxinus americana L. in air-dry storage caused a rapid decline in longevity and an increase in the rate of accumulation of chromosome aberrations. Storage of seeds fully imbibed but unable to germinate allowed a high germination capacity to be maintained for long periods, together with a very low incidence of chromosome aberrations. Seedlings grown from dry-stored seeds showed an increase in morphological abnormalities with length of storage, whereas seedlings from imbibed-stored seeds appeared normal. It is suggested that in dry tissues, enzyme-controlled turnover and repair may be temporarily suspended, and that this may be an important factor in the loss of seed viability in storage. The effect of increasing seed longevity by lowering the moisture content of dry-stored seeds is discussed in relation to this hypothesis. The relevance of the proposal is also discussed in relation to ecological studies. 相似文献