共查询到20条相似文献,搜索用时 46 毫秒
1.
Background
A 3-yr study examined whether prepartum treatment with casein hydrolyzate in combination with antibiotic, as routinely used in Israel for dry cow therapy, improved bacterial cure and increased milk yield in subsequent lactations in comparison with treatment with antibiotic alone. The vast majority of bacterial isolates in samples collected prior to drying-off comprised coagulase-negative staphylococci, mostly as Staph. chromogenes. 相似文献2.
Tanja Sigl Gregor Schlamberger Hermine Kienberger Steffi Wiedemann Heinrich HD Meyer Martin Kaske 《Acta veterinaria Scandinavica》2010,52(1):32
Background
Conjugated linoleic acid (CLA) is a collective term for isomers of octadecadienoic acid with conjugated double-bond system. Thus, it was the objective to investigate whether milk composition and metabolic key parameters are affected by adding CLA to the diet of dairy cows in the first four weeks of lactation. 相似文献3.
Torben W Bennedsgaard Stig M Thamsborg Frank M Aarestrup Carsten Enevoldsen Mette Vaarst Anna B Christoffersen 《Acta veterinaria Scandinavica》2006,48(1):24-6
Background
Quarter milk samples from cows with high risk of intramammary infection were examined to determine the prevalence of Staphylococcus aureus (SA) and penicillin resistant SA (SAr) in conventional and organic dairy herds and herds converting to organic farming in a combined longitudinal and cross-sectional study. 相似文献4.
High frequency of lactose intolerance in a prehistoric hunter-gatherer population in northern Europe
Helena Malmström Anna Linderholm Kerstin Lidén Jan Storå Petra Molnar Gunilla Holmlund Mattias Jakobsson Anders Götherström 《BMC evolutionary biology》2010,10(1):89
Background
Genes and culture are believed to interact, but it has been difficult to find direct evidence for the process. One candidate example that has been put forward is lactase persistence in adulthood, i.e. the ability to continue digesting the milk sugar lactose after childhood, facilitating the consumption of raw milk. This genetic trait is believed to have evolved within a short time period and to be related with the emergence of sedentary agriculture. 相似文献5.
Susana Delgado Rebeca Arroyo Esther Jiménez Maria L Marín Rosa del Campo Leonides Fernández Juan M Rodríguez 《BMC microbiology》2009,9(1):82
Background
AlthoughStaphylococcus aureusis considered the main etiological agent of infectious mastitis, recent studies have suggested that coagulase-negative staphylococci (CNS) may also play an important role in such infections. The aims of this work were to isolate staphylococci from milk of women with lactational mastitis, to select and characterize the CNS isolates, and to compare such properties with those displayed by CNS strains isolated from milk of healthy women. 相似文献6.
Branislav Lakic Ewa Wredle Kerstin Svennersten-Sjaunja Karin Östensson 《Acta veterinaria Scandinavica》2009,51(1):4
Background
A single prolonged milking interval (PMI) e.g. after a technical stop in an automated milking system is of concern for the producer since it is associated with a short-lasting increase in milk somatic cell count (SCC), which is a major quality criterion used at the dairy plants. The content of polymorphonuclear leukocytes (PMN) and how the milk quality is influenced has not been much investigated. The SCC peak occurs without any obvious antigen challenge, possibly indicating a different leukocyte attraction mechanism after a PMI than we see during mastitis. 相似文献7.
Background
Caseins, the main milk proteins, aggregate in the secretory pathway of mammary epithelial cells into large supramolecular structures, casein micelles. The role of individual caseins in this process and the mesostructure of the casein micelle are poorly known. 相似文献8.
Olivier Habimana Carine Le Goff Vincent Juillard Marie-Noëlle Bellon-Fontaine Girbe Buist Saulius Kulakauskas Romain Briandet 《BMC microbiology》2007,7(1):36
Background
The first step in biofilm formation is bacterial attachment to solid surfaces, which is dependent on the cell surface physico-chemical properties. Cell wall anchored proteins (CWAP) are among the known adhesins that confer the adhesive properties to pathogenic Gram-positive bacteria. To investigate the role of CWAP of non-pathogen Gram-positive bacteria in the initial steps of biofilm formation, we evaluated the physico-chemical properties and adhesion to solid surfaces of Lactococcus lactis. To be able to grow in milk this dairy bacterium expresses a cell wall anchored proteinase PrtP for breakdown of milk caseins. 相似文献9.
Laura Haltia Tuula Honkanen-Buzalski Irina Spiridonova Arvi Olkonen Vesa Myllys 《Acta veterinaria Scandinavica》2006,48(1):22-6
Background
Mastitis prevalence, milking procedures and management practices were investigated in 25 big dairy herds supplying milk to an Estonian dairy company. The aim of the study was to provide information for the company to be used in their new udder health improvement program to be set up after the completion of this study. 相似文献10.
Background
Shigella is the etiological agent of shigellosis, a disease responsible for more than 500,000 deaths of children per year, in developing countries. These pathogens colonize the intestinal colon, invade, spreading to the other enterocytes. Breastfeeding plays a very important role in protecting infants from intestinal infections. Amongst milk compounds, glycosylated proteins prevent the adhesion of many enteropathogens in vitro. The aim of this work was to determine the effect of human milk proteins on the colonization potential of Shigella dysenteriae, S. flexneri and S. sonnei. To fulfill this purpose, pooled milk samples from five donors, were fractionated by gel filtration and affinity chromatography. Using tissue culture, the milk fractions obtained were tested in Shigella adhesion and invasion assays. 相似文献11.
Background
The development of a new cold-active β-D-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. 相似文献12.
Fredrik Andersen Olav Østerås Olav Reksen Nils Toft Yrjo T Gröhn 《Acta veterinaria Scandinavica》2011,53(1):5
Background
This study was carried out to determine if an association exists between the shape of the lactation curve before it is influenced by the event of conception and the time from calving to conception in Norwegian dairy cattle. Lactation curves of Norwegian Red cows during 5 to 42 days in milk (DIM) were compared between cows conceiving between 43 and 93 DIM and cows conceiving after 93 DIM. 相似文献13.
Background
Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) is a significant disease among domestic and wild cattle. The BHV-1 infection was first detected in Finland in 1970; presumably it was imported in 1968. The infection reappeared in the large-scale bulk-tank milk surveillances which started in 1990, and was eradicated in 1994. Our aim is to describe the epidemiology of this infection in Finland, and its eradication. 相似文献14.
Traci Galbaugh Maria Grazia Cerrito Cynthia C Jose Mary Lou Cutler 《BMC cell biology》2006,7(1):34-15
Background
HC11 mouse mammary epithelial cells differentiate in response to lactogenic hormone resulting in expression of milk proteins including β-casein. Previous studies have shown that epidermal growth factor (EGF) blocks differentiation not only through activation of the Ras/Mek/Erk pathway but also implicated phosphatidylinositol-3-kinase (PI-3-kinase) signaling. The current study analyzes the mechanism of the PI-3-kinase pathway in an EGF-induced block of HC11 lactogenic differentiation. 相似文献15.
Acute heat stress brings down milk secretion in dairy cows by up-regulating the activity of the milk-borne negative feedback regulatory system 总被引:2,自引:0,他引:2
Background
The objective of this study was to determine if acute heat stress (HS) decreases milk secretion by activating the milk-borne negative feedback system, as an emergency physiological response to prevent a life-threatening situation. To induce HS, summer acclimatized dairy cows were exposed to full sun under mid-summer Mediterranean conditions, with and without conventional cooling procedures. 相似文献16.
Helen Huber Dominik Ziegler Valentin Pflüger Guido Vogel Claudio Zweifel Roger Stephan 《BMC veterinary research》2011,7(1):6
Background
Methicillin-resistant coagulase-negative staphylococci (MR-CNS) are of increasing importance to animal and public health. In veterinary medicine and along the meat and milk production line, only limited data were so far available on MR-CNS characteristics. The aim of the present study was to evaluate the prevalence of MR-CNS, to identify the detected staphylococci to species level, and to assess the antibiotic resistance profiles of isolated MR-CNS strains. 相似文献17.
ángel Pereira Rodríguez Fernández Rafael Leiro M Cristina Trillo M Esperanza Cerdán M Isabel González Siso Manuel Becerra 《Microbial cell factories》2006,5(1):41
Background
The β-galactosidase from Kluyveromyces lactis is a protein of outstanding biotechnological interest in the food industry and milk whey reutilization. However, due to its intracellular nature, its industrial production is limited by the high cost associated to extraction and downstream processing. 相似文献18.
Evaluation of DNA extraction methods for Bacillus anthracis spores isolated from spiked food samples
M.C. Thomas M.J. Shields K.R. Hahn T.W. Janzen N. Goji K.K. Amoako 《Journal of applied microbiology》2013,115(1):156-162
Aims
Nine commercial DNA extraction kits were evaluated for the isolation of DNA from 10‐fold serial dilutions of Bacillus anthracis spores using quantitative real‐time PCR (qPCR). The three kits determined by qPCR to yield the most sensitive and consistent detection (Epicenter MasterPure Gram Positive; MoBio PowerFood; ABI PrepSeq) were subsequently tested for their ability to isolate DNA from trace amounts of B. anthracis spores (approx. 6·5 × 101 and 1·3 × 102 CFU in 25 ml or 50 g of food sample) spiked into complex food samples including apple juice, ham, whole milk and bagged salad and recovered with immunomagnetic separation (IMS).Methods and Results
The MasterPure kit effectively and consistently isolated DNA from low amounts of B. anthracis spores captured from food samples. Detection was achieved from apple juice, ham, whole milk and bagged salad from as few as 65 ± 14, 68 ± 8, 66 ± 4 and 52 ± 16 CFU, respectively, and IMS samples were demonstrated to be free of PCR inhibitors.Conclusions
Detection of B. anthracis spores isolated from food by IMS differs substantially between commercial DNA extraction kits; however, sensitive results can be obtained with the MasterPure Gram Positive kit.Significance and Impact of the Study
The extraction protocol identified herein combined with IMS is novel for B. anthracis and allows detection of low levels of B. anthracis spores from contaminated food samples. 相似文献19.
Mahlokozera T Kang HH Goonetilleke N Stacey AR Lovingood RV Denny TN Kalilani L Bunn JE Meshnick SR Borrow P Letvin NL Permar SR;Center for HIV/AIDS Vaccine Immunology 《PloS one》2011,6(8):e23735
Background
The risk of postnatal HIV transmission is associated with the magnitude of the milk virus load. While HIV-specific cellular immune responses control systemic virus load and are detectable in milk, the contribution of these responses to the control of virus load in milk is unknown.Methods
We assessed the magnitude of the immunodominant GagRY11 and subdominant EnvKY9-specific CD8+ T lymphocyte response in blood and milk of 10 A*3002+, HIV-infected Malawian women throughout the period of lactation and correlated this response to milk virus RNA load and markers of breast inflammation.Results
The magnitude and kinetics of the HIV-specific CD8+ T lymphocyte responses were discordant in blood and milk of the right and left breast, indicating independent regulation of these responses in each breast. However, there was no correlation between the magnitude of the HIV-specific CD8+ T lymphocyte response and the milk virus RNA load. Further, there was no correlation between the magnitude of this response and markers of breast inflammation.Conclusions
The magnitude of the HIV-specific CD8+ T lymphocyte response in milk does not appear to be solely determined by the milk virus RNA load and is likely only one of the factors contributing to maintenance of low virus load in milk. 相似文献20.
Kasper A. Hettinga Fabiola M. Reina Sjef Boeren Lina Zhang Gerard H. Koppelman Dirkje S. Postma Jacques J. M. Vervoort Alet H. Wijga 《PloS one》2015,10(3)