Program and abstracts of the 6th Transgenic Technology Meeting (TT2005)

Abstracts

Program and abstracts of the 6th Transgenic Technology Meeting (TT2005)     

TT2013 meeting report: the Transgenic Technology meeting visits Asia for the first time

The 11th Transgenic Technology meeting was held in Guangzhou, China on 25th–27th February 2013. Over 300 scientists and students from round the world gathered to hear the latest developments in the technologies underpinning the creation of transgenic and knockout animals and their application to biological sciences in areas such as the modeling human diseases and biotechnology. As well as informative presentations from leading researchers in the field, an excellent selection of short talks selected from abstracts and posters, attendees were also treated to an inspiring talk from Allan Bradley who was awarded the 9th International Society of Transgenic Technologies Prize for outstanding contributions to the field of transgenic technologies.     

家畜转基因育种研究进展

转基因技术可以将外源基因导入家畜基因组,使其获得新的可遗传性状,为培育优良家畜品种提供了革命性途径。DNA显微注射法和体细胞克隆法是制备转基因家畜最常用的方法。应用转基因技术可以进行家畜抗病育种(抗病毒、抗菌和抗寄生虫),改良家畜的生产性状(胴体组成、奶品质、产毛、繁殖力和生长速度),培育环保型家畜新品种。文章从动物转基因技术入手,阐明其在家畜品种改良方面的研究现状和策略,并探讨家畜转基因育种面临的问题和应用前景。     

Program and Abstracts of the 10th Transgenic Technology Meeting (TT2011)

Abstracts

Program and Abstracts of the 10th Transgenic Technology Meeting (TT2011) TradeWinds Island Grand Resort, St Pete Beach, Florida, USA, October 24–26, 2011     

Program and Abstracts of the 9th Transgenic Technology Meeting (TT2010)

Abstracts

Program and Abstracts of the 9th Transgenic Technology Meeting (TT2010) 22–24 March 2010, Conference Centre, Max Delbrück Center for Molecular Medicine [MDC], Robert-R?ssle-Str. 10, 13125 Berlin, Germany     

转基因动物克隆技术的应用及生物反应器的建立

利用转基因克隆技术实现外源基因的导入宿主染色体基因组内稳定整合,并能遗传给后代,已在基因表达与调控的理论研究、人类遗传病动物模型的建立、药用蛋白的生产、抗病育种、人类移植用的器官的研究等方面得到广泛应用。转基因动物的研究与应用也已经成为21世纪生命科学领域最活跃、最具有实际应用价值的方向之一,尤其是作为生物反应器和医学上为人类提供所用器官方面,其经济价值和社会效益将是不可估量。在查阅大量近年来国内外相关资料的基础上,本文以转基因动物克隆为中心,对转基因动物克隆所采用显微注射技术、核移植技术、基因打靶与真核BAC表达载体制备等主要研究技术,以及转基因动物克隆在异种器官移植、构建生物反应器等方面的应用进行了综合性论述与分析,同时阐述了各种转基因技术的优点与缺点,以其为转基因动物克隆研究提供理论基础与技术支撑。     

The maize α-zein promoter can be utilized as a strong inducer of cellulase enzyme expression in maize kernels

Transgenic Research - Expression of recombinant proteins in plants is a technology for producing vaccines, pharmaceuticals and industrial enzymes. For the past several years, we have produced...     

The 7th Transgenic Technology meeting: debut for “down under” (http://www.tasq.uq.edu.au/TT2007)

The 7th Transgenic Technology meeting was held in Brisbane, Australia on February 12–14, 2007. Not only did this gathering mark a milestone as it was hosted outside the European continent for the first time, but also because it was the initial meeting to be held on behalf of the new International Society for Transgenic Technologies (ISTT, ). As in previous years, the topics were aimed towards both a scientific as well as a technical audience. The subjects covered a wide range of cutting edge applications in the field of genetic modifications in animal models, with the focus on (but by no means limited to) mice. True to the meetings tradition, a large emphasis was also laid on discussions about the management of transgenic production units. With the beautiful Australian sun shining over the venue, and a large number of exceptional speakers, this was a most pleasant and informative conference.     

Gene Targeting Approaches to Neuroendocrinology: Oxytocin, Maternal Behavior, and Affiliation

Transgenic technology affords exciting new opportunities in the field of behavioral neuroendocrinology. We have extended our research into the behavioral function of oxytocin in maternal and social behavior using two transgenic approaches: (i) targeted deletion of the oxytocin gene in mice and (ii) augmented oxytocin receptor expression in the brain. Mice genetically deficient in oxytocin can mate, give birth, and display normal maternal behavior; however, milk ejection and certain aspects of social behavior are affected. Comparative studies of oxytocin receptors have led to the observation that species differences in social organization are associated with differences in receptor distribution. Specifically, monogamous prairie voles and nonmonogamous, asocial montane voles exhibit different patterns of OT receptor expression in the brain. Transgenic mice have been created with a reporter gene driven by the prairie vole oxytocin receptor gene promoter. Analysis of the expression pattern suggests that it should be possible to manipulate receptor expression in the vole brain in order to examine the effects of receptor distribution on behavior.     

Transgenic Technology meeting 2008 in Toronto, Canada: a meeting report

In a history that goes back to 1999, the Transgenic Technology meetings started out in Sweden and over the years began to attract a growing community of technicians and researchers mainly from Europe. As the meetings started to attract an expanding worldwide audience, the community decided to found the International Society for Transgenic Technologies at the Barcelona meeting in 2005. The 2007 convention was held at Brisbane, Australia, and in 2008, the 8th Transgenic Technology meeting was held for the second time on behalf of ISTT and for the second time outside of Europe in Toronto, Canada. Due to its excellent program with over 400 participants the meeting was able to attract the highest number of delegates of all past TT meetings. With extended times for plenary discussions about technical and organizational aspects, as well as top level scientific presentations, both technicians and scientists enjoyed this as an extremely fruitful meeting from which they could take home solutions for daily routines as well as new insights and ideas for coming projects.     

转基因家蚕的研究进展及应用前景

转基因家蚕Bombyx mori是指利用分子生物学手段,将外源基因转移到家蚕染色体中, 使之出现先前不具有的性状和产物,并且可以保持传代,在个体水平可以体现外源基因的功能,使外源基因获得大量表达。目前转基因家蚕研究主要以piggyBac转座系统为常用载体, 绿色荧光蛋白(green fluorescent protein, GFP)基因为常用报告基因,经显微注射法获得转基因家蚕的成功率可达40%。通过转基因家蚕技术已经探明了家蚕外源导入核受体基因BmFtz-F1,调控家蚕体壁半透明的BmBLOS2基因、蜕皮启动激素(ecdysis-triggering hormone, ETH)基因以及家蚕抗菌肽CecB(cecropin B)基因的功能;获得了具有高品质、高细纤度、高拉伸强度和高弹性丝品种,能吐带绿色荧光或粉红色荧光的蚕丝品种, 抗家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus, BmNPV)品种及抗藤黄微球菌的品种;成功表达纯化了人的Ⅲ型前胶原蛋白、 人碱性纤维生长因子、人血清蛋白、人脑源性神经营养因子、人胰岛素生长因子Ⅰ、猫干扰素、单克隆抗体等生物活性蛋白、疫苗及特殊的生物材料。随着家蚕转基因技术的深入研究, 转基因家蚕产物将在国防、 军工、 航天、 医药等方面有着更为广阔的应用前景。     

不同控制条件下棉铃虫自然种群生命表研究

用作用因子组建的种群生命表方法组建了４种不同控制措施下的第３、４代棉铃虫自然种群生命表。结果表明,自然条件下第３、４代棉铃虫卵的寄生率分别为４．９３％、１．１９％,被捕食率平均２１．１１％和１５．８０％。第３、４代幼虫寄生率分别为１９．３４％、１６．２０％。结果表明,第３、４代棉铃虫自然种群增长指数分别仅为０．１９２１、０．１２２８;采用选择性杀虫剂ＩＫＩ和Ｂｔ制剂组合防治棉铃虫后。第３、４代种群     

Advances in transgenic animal models and techniques

On May 11th and 12th 2017 was held in Nantes, France, the international meeting “Advances in transgenic animal models and techniques” (http://www.trm.univ-nantes.fr/). This biennial meeting is the fifth one of its kind to be organized by the Transgenic Rats ImmunoPhenomic (TRIP) Nantes facility (http://www.tgr.nantes.inserm.fr/). The meeting was supported by private companies (SONIDEL, Scionics computer innovation, New England Biolabs, MERCK, genOway, Journal Disease Models and Mechanisms) and by public institutions (International Society for Transgenic Technology, University of Nantes, INSERM UMR 1064, SFR François Bonamy, CNRS, Région Pays de la Loire, Biogenouest, TEFOR infrastructure, ITUN, IHU-CESTI and DHU-Oncogeffe and Labex IGO). Around 100 participants, from France but also from different European countries, Japan and USA, attended the meeting.     

Long-term stability of marker gene expression in Prunus subhirtella: a model fruit tree species

Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumno rosa (PAR) was chosen as model fruit tree species and transformed with a reporter gene (uidA) under the control of the 35S promoter. Using Southern and GUS fluorometric techniques, we compared transgene copy numbers and observed stability of transgene expression levels in 34 different transgenic plants, grown under in vitro, greenhouse and screenhouse conditions, over a period of 9 years. An influence of grafting on gene expression was not observed. No silenced transgenic plant was detected. Overall, these results suggest that transgene expression in perennial species, such as fruit trees, remains stable in time and space, over extended periods and in different organs, confirming the value of PAR as model species to study season-dependent regulation in mature stone fruit tissues. While the Agrobacterium-derived Prunus transformants contained one to two copies of the transgenes, 91% of the transgenic events also contained various lengths of the bacterial plasmid backbone, indicating that the Agrobacterium-mediated transformation is not as precise as previously perceived. The implications for public acceptance and future applications are discussed.     

Improved reactive aldehyde,salt and cadmium tolerance of transgenic barley due to the expression of aldo–keto reductase genes

Under various stress conditions, plant cells are exposed to oxidative damage which triggers lipid peroxidation. Lipid peroxide breakdown products include protein crosslinking reactive aldehydes. These are highly damaging to living cells. Stress-protective aldo–keto reductase (AKR) enzymes are able to recognise and modify these molecules, reducing their toxicity. AKRs not only modify reactive aldehydes but may synthesize osmoprotective sugar alcohols as well. The role of these mixed function enzymes was investigated under direct reactive aldehyde, heavy metal and salt stress conditions. Transgenic barley (Hordeum vulgare L.) plants constitutively expressing AKR enzymes derived from either thale cress (Arabidopsis thaliana) (AKR4C9) or alfalfa (Medicago sativa) (MsALR) were studied. Not only AKR4C9 but MsALR expressing plants were also found to produce more sorbitol than the non-transgenic (WT) barley. Salinity tolerance of genetically modified (GM) plants improved, presumably as a consequence of the enhanced sorbitol content. The MsALR enzyme expressing line (called 51) exhibited almost no symptoms of salt stress. Furthermore, both transgenes were shown to increase reactive aldehyde (glutaraldehyde) tolerance. Transgenic plants also exhibited better cadmium tolerance compared to WT, which was considered to be an effect of the reduction of reactive aldehyde molecules. Transgenic barley expressing either thale cress or alfalfa derived enzyme showed improved heavy metal and salt tolerance. Both can be explained by higher detoxifying and sugar alcohol producing activity. Based on the presented data, we consider AKRs as very effective stress-protective enzymes and their genes provide promising tools in the improvement of crops through gene technology.     

转基因技术在水产动物营养中的应用

近年来,随着分子生物学研究的进展,转基因技术得到了迅猛的发展,在水产动物营养中也有广泛的应用.动物转基因技术是指将外源基因或体外重组的基因结构导入动物的基因组内,使其在动物体内整合和表达,产生具有新的遗传性状,并能将这种性状稳定地传递给后代的一种技术.对转基因技术的一般方法,如显微注射、精子载体、电穿孔,逆转录病毒载体感染和基因枪等方法,以及转基因技术在水产动物营养中的应用进行了综述.     

6th Transgenic Technology Meeting Report (http://www.cnb.uam.es/~tt2005)

The 6th Transgenic Technology Meeting was held in Barcelona, September 2005. The meeting in Barcelona was one of the largest in TTM series since its inception in Stockholm in 1999. Sessions held included topics on new transgenics technologies; the generation and breeding of transgenics and knockout mice; functional genomics; analysis of genetically modified mice and mouse genetics and development.     

Transgenic plants as protein factories

Transgenic plants are showing considerable potential for the economic production of proteins, with a few already being marketed. Recent clinical trials of pharmaceuticals produced from transgenic plants are encouraging, with plant glycans showing reassuringly poor immunogenicity. Our increasing understanding of protein targeting and accumulation should further improve the potential of this new technology.     

Mighty mice: Transgenic technology “knocks out” questions of matrix metalloproteinase function

Matrix metalloproteinases (MMP) comprise a family of structurally related proteinases that are believed to play a critical role in many physiological and pathological processes. Transgenic technology offers the possibility of determining whether MMPs contribute directly to these processes. For example, gain of function and loss of function models have confirmed causative roles of MMPs in the development of pulmonary emphysema and unexpectedly uncovered an MMP. dependent mechanism of inflammatory cell recruitment. Limitations of these techniques and powerful applications on the horizon are also presented as we embark on an era where controlled experiments can be performed in complex mammalian models.