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1.
Numerous studies have illustrated that low light is one of the major abiotic stresses limiting cotton ( Gossypium hirsutum L.) fiber length, but studies addressing molecular mechanisms contributing to reduced fiber growth under low light are lacking. To investigate the molecular mechanisms of cotton fiber elongation in response to low light, an experiment of low light caused by shading was conducted with cotton cultivar NuCOTN 33B. The results showed that low light resulted in shorter fiber length. Proteomic analysis of four developmental stages (5, 10, 15 and 20 days post-anthesis) showed that 49 proteins were significantly responsive to low light. 39 differentially expressed proteins that included some known as well as some novel low-light stress-responsive proteins were identified. These differentially expressed proteins were involved in signal transduction, carbohydrate/energy metabolism, cell wall component synthesis, protein metabolism, cytoskeleton, nitrogen metabolism and stress responses. The results also showed that the decrease in fiber length might be because the levels of signal-related protein (phospholipase D), cytoskeletal proteins (two annexins isoforms), cell wall component-related proteins (sucrose synthase, UDP- d-glucuronic acid 4-epimerase and rhamnose synthase), carbohydrate metabolism-proteins (phosphofructokinase, dihydrolipoamide dehydrogenase, vacuolar H +-ATPase catalytic subunit, malate dehydrogenase and isocitrate dehydrogenase), and stress-related proteins (peroxisomal catalase, short chain alcohol dehydrogenase) were decreased under low light. 相似文献
2.
Low temperature stress is one of the major abiotic stresses limiting the formation of cotton ( Gossypium hirsutum L.) fiber qualities, especially fiber length. To investigate the molecular adaptation mechanisms of cotton fiber elongation to low temperature stress, two cotton cultivars, Kemian 1 (low temperature-tolerant) and Sumian 15 (low temperature-sensitive), were planted in the field at two sowing dates (25 April and 10 June). The two sowing dates resulted in different growing conditions and the main environmental difference between them was temperature, particularly the mean daily minimum temperature (MDTmin). When the sowing date was delayed, the MDTmin decreased from 26.9 °C (25 April) to 20.6 °C (10 June). Low temperature stress (MDTmin of 20.6 °C) shortened the fiber length significantly in two cultivars, but the decreased extent was larger in Sumian 15 than that in Kemian 1. Proteomic analysis of three developmental stages (10, 15 and 20 days post-anthesis [DPA]) showed that 37 spots changed significantly ( p < 0.05) in abundance under low temperature stress and they were identified using mass spectrometry. These proteins were involved in malate metabolism, soluble sugar metabolism, cell wall loosening, cellulose synthesis, cytoskeleton, cellular response, and redox homeostasis. The results suggest that the enhancement of osmoticum maintenance, cell wall loosening, cell wall components biosynthesis, and cytoskeleton homeostasis plays important roles in the tolerance of cotton fibers to low temperature stress. Moreover, low levels of PEPCase, expansin, and ethylene signaling proteins may potentially lead to the low temperature sensitivity of Sumian 15 at the proteomic level. 相似文献
3.
NuCOTN 33B, a Bt transgenic variety of upland cotton (Gossypium hirsutum L.) expressing the insecticidal protein Cry1Ac from Bacillus thuringiensis Berliner sp. kurstaki, was evaluated for resistance to Helicoverpa armigera (Hübner) during 1998-2000 in northern China. The results indicated that there was no significant difference in egg densities between NuCOTN 33B and three nontransgenic varieties (DP5415, Zhongmian12, and Shiyuan321) during the season, although the survival of larvae on NuCOTN 33B seemed significantly reduced. High larval densities observed on non-Bt cotton appeared in great contrast to the low larval populations observed on NuCOTN 33B plants during the seasons. In an environment without insecticide sprays, the annual ginned cotton yields in NuCOTN 33B plots, ranging from 1391.17 to 1511.35 kg/ha, were significantly higher than those in non-Bt cotton (340.34-359.58 kg/ha). These high levels of field efficacy for NuCOTN 33B against H. armigera in northern China may pave the way for reduced pesticide applications and an expansion of alternative pest-control strategies. 相似文献
4.
Cotton ( Gossypium hirsutum L.) is a major crop and the main source of natural fiber worldwide. Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality, improved stress resistance of this crop plant is urgently needed. In this study, we used Gateway technology to construct a normalized full‐length cDNA overexpressing (FOX) library from upland cotton cultivar ZM12 under various stress conditions. The library was transformed into Arabidopsis to produce a cotton‐FOX‐Arabidopsis library. Screening of this library yielded 6,830 transgenic Arabidopsis lines, of which 757 were selected for sequencing to ultimately obtain 659 cotton ESTs. GO and KEGG analyses mapped most of the cotton ESTs to plant biological process, cellular component, and molecular function categories. Next, 156 potential stress‐responsive cotton genes were identified from the cotton‐FOX‐Arabidopsis library under drought, salt, ABA, and other stress conditions. Four stress‐related genes identified from the library, designated as GhCAS, GhAPX, GhSDH, and GhPOD, were cloned from cotton complementary DNA, and their expression patterns under stress were analyzed. Phenotypic experiments indicated that overexpression of these cotton genes in Arabidopsis affected the response to abiotic stress. The method developed in this study lays a foundation for high‐throughput cloning and rapid identification of cotton functional genes. 相似文献
5.
Although the fibers of cotton ( Gossypium hirsutum L.) are single cells with a secondary wall composed primarily of cellulose, the cell-wall polymers of the fibers are technically difficult to characterize with respect to molecular weights. This limitation hinders understanding how the fiber wall composition changes during development, particularly with respect to genotypic variations, and how the molecular composition is related to physical properties. We analyzed cell-wall polymers from cotton fibers (cultivar, Texas Marker-1) at several developmental stages (8–60 days post-anthesis; DPA) by gel-permeation chromatography of components soluble in dimethyl acetamide and lithium chloride. This procedure solubilizes fiber cell-wall components directly without prior extraction or derivatization, processes that could lead to degradation of high-molecular-weight components. Cellwall polymers from fibers at primary cell-wall stages had lower molecular weights than the cellulose from fibers at the secondary wall stages; however, the high-molecularweight cellulose characteristic of mature cotton was detected as early as 8 DPA. High-molecular-weight material decreased during the period of 10–18 DPA with concomitant increase in lower-molecular-weight wall components, possibly indicating hydrolysis during the later stages of elongation.Abbreviations DMAC
dimethyl acetamide
- DP
degree of polymerization
- DPA
days post anthesis
- GPC
gel-permeation chromatography
- MW
molecular weight
- MWD
molecular-weight distribution
- TM-1
Texas Marker 1 相似文献
6.
Transgenic Bt cotton, engineered to continuously produce activated delta-endotoxins of the soil bacteria Bacillus thuringiensis, holds great promise in controlling Helicoverpa armigera and other lepidopteran pests. However, it also may impact the invertebrate community, which needs to be clarified. The effects of Bt cotton on two nontarget insects, Aphis gossypii and Orius sauteri, were assessed under semifield and laboratory conditions. Mean total duration of nymphal stages of A. gossypii was shorter (5.9 versus 6.3 d), and rm was higher (0.418 versus 0.394) on conventional Simian 3 (the most frequently planted non-Bt cotton in northern China) than on Bt transgenic NuCOTN 33B (the first Bt cotton commercially planted in China). Mean duration of fourth-instar O. sauteri was significantly longer on transgenic GK-12 (3.7 d) than on NuCOTN 33B (3.2 d), but no different from Simian 3. Mean total mortality was significantly lower on Simian 3 (3.7%) than on GK-12 (14.8%). During the fourth instar, the predator consumed a significantly higher number of prey on Simian 3 (202.3 prey) than on NuCOTN 33B (159.0), whereas the mean total number of A. gossypii prey consumed during the nymphal stage was significantly higher on Simian 3 (336.8 prey) and GK-12 (330.3 prey) than on NuCOTN 33B (275.7). No detrimental effects were detected on development (nymphs, adults, and progeny eggs), fecundity, longevity, and egg viability of O. sauteri on Bt cotton aphids compared with non-Bt cotton aphids. These results suggest that Bt cotton cultivars GK-12 and NuCOTN 33B have no direct effect on nontargets A. gossypii and O. sauteri. Germplasm divergence may account for the negative effects observed on A. gossypii and O. sauteri when reared on NuCOTN 33B or NuCOTN 33B-fed aphids. The biological meanings of the small difference observed between GK-12 and Simian 3 on survival of O. sauteri will require close monitoring over longer time periods. 相似文献
7.
In our previous study, we used a comparative proteomic approach based on 2DE to profile dynamic proteomes of cotton fibers and found 235 protein spots differentially expressed during the elongation process ranging from 5 to 25 days post‐anthesis. Of them, only 106 differentially expressed proteins (DEPs) were identified by MS due to database limitations at the time. In the present work, we successfully identified the remaining 129 DEPs from the same experimental system using high‐resolution MS with an updated database. Bioinformatic analysis revealed that proteins involved in carbohydrate and protein metabolism, transport, and redox homeostasis are the most abundant, and glycolysis was found to be the most significantly regulated process during fiber elongation. Our high‐confidence reference dataset, composed of 235 DEPs, provides a valuable resource for future studies on the molecular mechanism of cotton fiber elongation. 相似文献
8.
Cotton is one of the most important crops for its natural textile fibers in the world. However, it often suffered from drought stress during its growth and development, resulting in a drastic reduction in cotton productivity. Therefore, study on molecular mechanism of cotton drought-tolerance is very important for increasing cotton production. To investigate molecular mechanism of cotton drought-resistance, we employed RNA-Seq technology to identify differentially expressed genes in the leaves of two different cultivars (drought-resistant cultivar J-13 and drought-sensitive cultivar Lu-6) of cotton. The results indicated that there are about 13.38% to 18.75% of all the unigenes differentially expressed in drought-resistant sample and drought-sensitive control, and the number of differentially expressed genes was increased along with prolonged drought treatment. DEG (differentially expression gene) analysis showed that the normal biophysical profiles of cotton (cultivar J-13) were affected by drought stress, and some cellular metabolic processes (including photosynthesis) were inhibited in cotton under drought conditions. Furthermore, the experimental data revealed that there were significant differences in expression levels of the genes related to abscisic acid signaling, ethylene signaling and jasmonic acid signaling pathways between drought-resistant cultivar J-13 and drought-sensitive cultivar Lu-6, implying that these signaling pathways may participate in cotton response and tolerance to drought stress. 相似文献
9.
To understand the complex drought response mechanism in crop plants, a systematic root proteomics approach was adopted to
identify and analyze the expression patterns of differentially expressed major root proteins of Vigna radiata during short-term (3 days) and consecutive long-term water-deficit (6 days) as well as during recovery (6 days after re-watering).
Photosynthetic gas exchange parameters of the plant were measured simultaneously during the stress treatment and recovery
period. A total of 26 major protein spots were successfully identified by mass spectrometry, which were grouped according
to their expression pattern during short-term stress as significantly up-regulated (9), down-regulated (10), highly down-regulated,
beyond detection level of the software (2) and unchanged (5). The subsequent changes in the expression patterns of these proteins
during long-term stress treatment and recovery period was analyzed to focus on the dynamic regulation of these functionally
important proteins during progressive drought and recovery period. Cytoskeleton-related proteins were down-regulated initially
(3d) but regained their expression levels during subsequent water-deficit (6d) while glycoprotein like lectins, which were
primarily known to be involved in legume–rhizobia symbiosis, maintained their enhanced expression levels during both short
and long-term drought treatment indicating their possible role in drought stress response of legumes. Oxidative stress-related
proteins including Cu/Zn superoxide dismutase, oxidoreductase and aldehyde reductase were also up-regulated. The analyses
of the dynamic regulation of these root proteins during short- and long-term water-deficit as well as recovery period may
prove crucial for further understanding of drought response mechanisms in food legumes. 相似文献
12.
为研究棉花纤维起始和伸长的分子机理,在棉花纤维EST序列分析的基础上,从棉花纤维中扩增并克隆了2个棉花Rac蛋白的cDNA基因,分别命名为GhRacA和GhRacB。GhRacA cDNA长959bp,推测的编码蛋白包含211个氨基酸。GhRacB cDNA长920bp,编码195个氨基酸的蛋白。GhRacA和GhRacB蛋白均含有GTP/GDP结合和激活区域、Effector区和碱性氨基酸区。GhRacB的C末端有保守的异戊烯基化位点CSIL,而GhRacA没有明显的异戊烯基化位点。序列比较分析表明,GhRacA和GhRacB是2个新的棉花Rac蛋白。RT-PCR分析表明,GhRacA和GhRacB在根、下胚轴、茎、叶和纤维中都有表达,但均在棉花纤维起始和伸长时期有优势表达,推测2个基因在棉花纤维的早期发育中可能有重要的功能。 相似文献
13.
To understand the effects of drought on fennel seed production and determine the underlying molecular processes, various fennel genotypes were exposed to drought stress. The yield and quality, including aromatic oil content, of fennel seeds were reduced by drought during seed development. To explore drought-induced biological processes in fennel, a label-free/gel-free proteomic analysis was performed. In Gaziantep and Tatmaj cultivars, which are sensitive and tolerant fennel genotypes, respectively, 106 and 92 drought-responsive proteins were identified. Comparison of protein-functional profiles indicated that proteins classified in stress, cell, and protein synthesis/degradation categories consisted important responsive mechanisms against drought stress. Pathway analysis visualized that the tricarboxylic acid cycle is important for both cultivars. In Tatmaj, moderate activation of proteins related to oxidative pentose phosphate pathway was detected along with an increase in photosynthesis-related proteins. Furthermore, cluster analysis of drought-responsive proteins using protein abundance at milky, dough, and mature stages identified protein homeostasis as a mechanism of drought tolerance in fennel. These results suggest that coordinated energy consumption and supply might be a drought-tolerance mechanism in fennel plants. 相似文献
14.
Nitrogen (N) is an essential macronutrient and an important factor limiting agricultural productivity. N deficient or excess conditions often occur during the cotton growth season and incorrect N application may affect cotton fiber yield and quality. Here, the influence of N stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spectrometry. The results indicated that N application rate affects nitrogen accumulation in fiber cells and fiber length. The proteins differentially expressed during N stress were mainly related to plant carbohydrate metabolism, cell wall component synthesis and transportation, protein/amino acid metabolism, antioxidation and hormone metabolism. The most abundant proteins were C metabolism-related. Ten days post anthesis is a critical time for fiber cells to perceive environmental stress and most proteins were suppressed in both N deficient and N excess conditions at this sampling stage. However, several N metabolism proteins were increased to enhance N stress tolerance. Excess N may suppress carbohydrate/energy metabolism in early fiber development much like N deficiency. These results have identified some interesting proteins that can be further analyzed to elucidate the molecular mechanisms of N tolerance. 相似文献
15.
Drought is one of the most important constraints on the growth and productivity of many crops, including soybeans. However, as a primary sensing organ, the plant root response to drought has not been well documented at the proteomic level. In the present study, we carried out a proteome analysis in combination with physiological analyses of soybean roots subjected to severe but recoverable drought stress at the seedling stage. Drought stress resulted in the increased accumulation of reactive oxygen species and subsequent lipid peroxidation. The proline content increased in drought-stressed plants and then decreased during the period of recovery. The high-resolution proteome map demonstrated significant variations in about 45 protein spots detected on Comassie briliant blue-stained 2-DE gels. Of these, 28 proteins were identified by mass spectrometry; the levels of 5 protein spots were increased, 21 were decreased and 2 spots were newly detected under drought condition. When the stress was terminated by watering the plants for 4 days, in most cases, the protein levels tended towards the control level. The proteins identified in this study are involved in a variety of cellular functions, including carbohydrate and nitrogen metabolism, cell wall modification, signal transduction, cell defense and programmed cell death, and they contribute to the molecular mechanism of drought tolerance in soybean plants. Analysis of protein expression patterns revealed that proteins associated with osmotic adjustment, defense signaling and programmed cell death play important roles for soybean plant drought adaptation. The identification of these proteins provides new insight that may lead to a better understanding of the molecular basis of the drought stress responses. 相似文献
16.
Cotton (Gossypium herbaceum L.) fibers elongated almost linearly up to about 20 days post anthesis. The molecular mass of xyloglucans in fiber cell walls decreased gradually during the elongation stage. When enzymatically active (native) cell wall preparations of fibers were autolyzed, the molecular mass of xyloglucans decreased. The decrease was most prominent in wall preparations obtained from the rapidly elongating fibers. The xyloglucan-degrading activity was recovered from the fiber cell walls with 3 mol/L NaCl, and the activity was high at the stages in which fibers elongated vigorously. These results suggest the possible involvement of xyloglucan metabolism in the regulation of cotton fiber elongation. 相似文献
17.
在池栽条件下,以美棉33B为材料,研究花铃期干旱、渍水7 d和渍水14 d处理对棉铃碳水化合物含量的影响及其与生物量累积的关系.结果表明: 干旱处理对下部果枝棉铃铃壳碳水化合物含量的影响较小,降低了中部铃壳碳水化合物含量;干旱处理上部铃壳和渍水处理铃壳可溶性糖、淀粉含量增加,蔗糖含量先降后增,且随渍水持续期延长变幅增大,蔗糖外运受阻加重.与铃壳相比,花铃期干旱和渍水处理对棉籽碳水化合物含量的影响较小.干旱和渍水7 d处理中部果枝棉铃生物量快速增长起始期提前但历时短,下部和上部棉铃生物量累积最大增长速率降低;渍水14 d处理不同果枝部位棉铃生物量累积最大增长速率均降低.相关分析表明,棉铃生物量及其最大增长速率与铃壳可溶性糖和蔗糖含量关系密切.因此,花铃期干旱和渍水影响棉铃蔗糖外运,改变棉铃生物量累积特性,是棉铃生物量降低的原因.
相似文献
20.
Drought is the primary limitation to plant growth and yield in agricultural systems. Cucumber ( Cucumis sativus) is one of the most important vegetables worldwide and has little tolerance for water deficit. To understand the drought stress response strategy of this plant, the responses of cucumber to short‐term drought and rewatering were determined in this study by morphological structure and proteomic analyses. The leaf relative water content was significantly decreased under drought, and the cell structure was altered, while rewatering obviously alleviated the symptoms of water shortage and cell damage. A total of 320 and 246 proteins exhibiting significant abundance changes in response to drought and recovery, respectively, were identified. Our proteome analysis showed that 63 co‐regulated proteins were shared between drought and rewatering, whereas most of the responsive proteins were unique. The proteome is adjusted through a sequence of regulatory processes including the biosynthesis of secondary metabolites and the glutathione metabolism pathway, which showed a high correlation between protein abundance profile and corresponding enzyme activity. Drought and recovery regulated different types of proteins, allowing plants to adapt to environmental stress or restore growth, respectively, which suggests that short‐term drought and recovery are almost fully uncoupled processes. As an important component of the antioxidant system in plants, glutathione metabolism may be one of the main strategies for regulating antioxidant capacity during drought recovery. Our results provide useful information for further analyses of drought adaptability in cucumber plants. 相似文献
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