共查询到20条相似文献,搜索用时 46 毫秒
1.
From silencing to gene expression: real-time analysis in single cells 总被引:28,自引:0,他引:28
Janicki SM Tsukamoto T Salghetti SE Tansey WP Sachidanandam R Prasanth KV Ried T Shav-Tal Y Bertrand E Singer RH Spector DL 《Cell》2004,116(5):683-698
2.
3.
4.
5.
6.
7.
Long-range nucleosome ordering is associated with gene silencing in Drosophila melanogaster pericentric heterochromatin
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We have used line HS-2 of Drosophila melanogaster, carrying a silenced transgene in the pericentric heterochromatin, to investigate in detail the chromatin structure imposed by this environment. Digestion of the chromatin with micrococcal nuclease (MNase) shows a nucleosome array with extensive long-range order, indicating regular spacing, and with well-defined MNase cleavage fragments, indicating a smaller MNase target in the linker region. The repeating unit is ca. 10 bp larger than that observed for bulk Drosophila chromatin. The silenced transgene shows both a loss of DNase I-hypersensitive sites and decreased sensitivity to DNase I digestion within an array of nucleosomes lacking such sites; within such an array, sensitivity to digestion by MNase is unchanged. The ordered nucleosome array extends across the regulatory region of the transgene, a shift that could explain the loss of transgene expression in heterochromatin. Highly regular nucleosome arrays are observed over several endogenous heterochromatic sequences, indicating that this is a general feature of heterochromatin. However, genes normally active within heterochromatin (rolled and light) do not show this pattern, suggesting that the altered chromatin structure observed is associated with regions that are silent, rather than being a property of the domain as a whole. The results indicate that long-range nucleosomal ordering is linked with the heterochromatic packaging that imposes gene silencing. 相似文献
8.
9.
10.
11.
12.
13.
14.
15.
16.
The gene encoding the mouse contactin‐1 axonal glycoprotein is regulated by the collier/Olf1/EBF family early B‐Cell factor 2 transcription factor
下载免费PDF全文
![点击此处可从《Developmental neurobiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Antonella Bizzoca Sabrina Picocci Patrizia Corsi Stefania Arbia Laura Croci G. Giacomo Consalez Gianfranco Gennarini 《Developmental neurobiology》2015,75(12):1420-1440
17.
18.
19.