Habitat usage of Daubenton's bat (Myotis daubentonii), common pipistrelle (Pipistrellus pipistrellus), and soprano pipistrelle (Pipistrellus pygmaeus) in a North Wales upland river catchment

Distributions of Daubenton's bat (Myotis daubentonii), common pipistrelle, (Pipistrellus pipistrellus), and soprano pipistrelle (Pipistrellus pygmaeus) were investigated along and altitudinal gradient of the Lledr River, Conwy, North Wales, and presence assessed in relation to the water surface condition, presence/absence of bank‐side trees, and elevation. Ultrasound recordings of bats made on timed transects in summer 1999 were used to quantify habitat usage. All species significantly preferred smooth water sections of the river with trees on either one or both banks; P. pygmaeus also preferred smooth water with no trees. Bats avoided rough and cluttered water areas, as rapids may generate high‐frequency echolocation‐interfering noise and cluttered areas present obstacles to flight. In lower river regions, detections of bats reflected the proportion of suitable habitat available. At higher elevations, sufficient habitat was available; however, bats were likely restricted due to other factors such as a less predictable food source. This study emphasizes the importance of riparian habitat, bank‐side trees, and smooth water as foraging habitat for bats in marginal upland areas until a certain elevation, beyond which bats in these areas likely cease to forage. These small‐scale altitudinal differences in habitat selection should be factored in when designing future bat distribution studies and taken into consideration by conservation planners when reviewing habitat requirements of these species in Welsh river valleys, and elsewhere within the United Kingdom.     

Scale-Dependent Effects of Landscape Structure and Composition on Diurnal Roost Selection by Forest Bats

Abstract: Forest management affects the quality and availability of roost sites for forest-dwelling bats, but information on roost selection beyond the scale of individual forest stands is limited. We evaluated effects of topography (elevation, slope, and proximity of roads and streams), forest habitat class, and landscape patch configuration on selection of summer diurnal roosts by 6 species of forest-dwelling bats in a diverse forested landscape of the Ouachita Mountains, Arkansas, USA. Our objectives were to identify landscape attributes that potentially affect roost placement, determine whether commonalities exist among species in their response to landscape attributes, and evaluate the effects of scale. We modeled roost selection at 2 spatial scales (250- and 1,000-m radius around each roost). For each species, parameters included in models differed between the 2 scales, and there were no shared parameters for 2 species. Average coefficients of determination (R²) for small-scale models were generally higher than for large-scale models. Abundance of certain forest habitat classes were included more often than patch configuration or topography in differentiating roost from random locations, regardless of scale, and most species were more likely to roost in areas containing abundant thinned forest. Among topographic metrics, big brown bats (Eptesicus fuscus) were more likely to roost at higher elevations; roosts of big brown bats, northern long-eared bats (Myotis septentrionalis), and Seminole bats (Lasiurus seminolus) were influenced by slope; and big brown bats, evening bats (Nycticeius humeralis), and Seminole bats were more likely to roost closer to water than random. Northern long-eared bats and red bats (Lasiurus borealis) were more likely to roost closer to roads, whereas eastern pipistrelles (Perimyotis subflavus) were more likely to roost further from roads than random. Common parameters in most models included 1) positive associations with group selection (5 of 6 species) and thinned mature forest (4 species) at the small scale; 2) negative associations with unmanaged mixed pine-hardwood forest 50–99 years old at the large scale (4 species); 3) negative association with stands of immature pine 15–29 years old at the small scale (3 species); and 4) a positive association with largest patch index at the large scale (3 species). Our results suggest that, in a completely forested landscape, a variety of stand types, seral stages, and management conditions, varying in size and topographic location throughout the landscape, would likely provide the landscape components for roosting required to maintain a diverse community of forest bats in the Ouachita Mountains.     

Feeding Territoriality in Female Northern Bats,Eptesicus nilssoni

A nursing colony of 26 Northern bats was studied in South-Central Sweden. The foraging behaviour is described with emphasis on social interactions on feeding grounds. The bats foraged in small feeding territories used night after night. The reproducing females defended feeding territories against other colony members as well as non-members by means of aggressive chases and vocalizations. A linear dominance order occurred among the females that regularly visited a feeding site. Intrusions into already occupied feeding sites resulted in territorial conflicts (47 %), passive departure by one or both opponents (43%) or mutual acceptance (11%). Conflicts occurred throughout the summer, but became less frequent in July when insect abundance increased.     

Differential mobility in two small phyllostomid bats,<Emphasis Type="Italic">Artibeus watsoni</Emphasis> and<Emphasis Type="Italic">Micronycteris microtis</Emphasis>, in a fragmented neotropical landscape

To assess the influence of habitat fragmentation on small bats, we determined home range size and mobility of the frugivorousArtibeus watsoni Thomas, 1901 and the gleaning insectivorousMicronycteris microtis Miller, 1898 by radiotracking on different-sized islands (2.7–17 ha) in Lake Gatún, Panamá. The two species differed in their response to fragmentation. Home range size was highly variable in the five trackedA. watsoni, ranging from 1.8 to 17.9 ha with a mean of about 9 ha. Some individuals flew regularly between islands and/or the mainland, thereby traversing up to 180 m of open water. In comparison, home ranges of threeM. microtis were with about 3.8 ha only half as large. All ofM. microtis exhibited sedentary foraging behaviour and did not cross open water, suggesting that they might persist at least on some of the islands as resident populations. Our findings are consistent with radiotracking data from a previous study and indicate that small habitat patches are still used by small bats, provided the degree of isolation is low and that sufficient resources and larger habitat patches exist in close vicinity, potentially acting as additional feeding grounds and source populations.     

Impact of landscape fragmentation on a specialised woodland bat,Rhinolophus hipposideros

For habitat specialists, fragmentation has major consequences as it means less suitable habitat for the species to live in. In a fragmented landscape, we would expect larger, but spatially more clustered, foraging ranges. We studied the impact of landscape fragmentation on the foraging range and habitat exploitation of a specialised forest bat by radiotracking 16 female lesser horseshoe bats Rhinolophus hipposideros in a landscape with connected woodland structures and in a highly fragmented landscape in Carinthia, Austria.Contrary to our expectations, spatial foraging behaviour was not influenced by fragmentation. No differences in the behaviour of the bats between the sites were evident for the foraging ranges (minimum convex polygon, MCP), the core foraging areas (50% kernel), nor the mean or the maximum distances from the roost. However, in the highly fragmented landscape, the foraging activity of individuals was spatially more clustered and the overall MCP of all bats of a colony was greater compared to the less fragmented landscape.Woodland was the most important foraging habitat for the lesser horseshoe bats at both study sites. Habitat selection at the individual MCPs was evident only at the site with low fragmentation. However, in the core foraging areas, woodland was significantly selected over all other habitat types at both study sites.We conclude that (1) conservation measures for colonies of lesser horseshoe bats should be undertaken within 2.5 km of the nursery roost, (2) woodland is the key foraging habitat particularly in the vicinity of the roost, and (3) any loss of woodland near the colonial roosts are likely to negatively influence the colony, since these bats do not seem to be able to adapt their spatial foraging behaviour in a degraded landscape. The inflexible spatial behaviour of this specialised bat highlights the need to compensate for any habitat loss within the foraging range of a bat colony.     

Evening Bat Summer Roost-Site Selection on a Managed Pine Landscape

ABSTRACT Creation and maintenance of forested corridors to increase landscape heterogeneity has been practiced for decades but is a new concept in intensively managed southern pine (Pinus spp.) forests. Additionally, more information is needed on bat ecology within such forest systems. Therefore, we examined summer roost-site selection by evening bats (Nycticeius humeralis) in an intensively managed landscape with forested corridors in southeastern South Carolina, USA, 2003–2006. We radiotracked 53 (26 M, 27 F) adult evening bats to 75 (31 M, 44 F) diurnal roosts. We modeled landscape-level roost-site selection with logistic regression and evaluated models using Akaike's Information Criterion for small samples. Model selection results indicated that mature (≥40 yr) mixed pine-hardwood stands were important roost sites for male and lactating female evening bats. Upland forested corridors, comprised of mature pine or mixed pine-hardwoods, were important roosting habitats for males and, to a lesser extent, lactating females. Male roosts were farther from open stands and lactating female roosts were farther from mid-rotation stands than randomly selected structures. Our results suggest roost structures (i.e., large trees and snags) in mature forests are important habitat components for evening bats. We recommend maintaining older (>40 yr old) stand conditions in the form of forest stands or corridors across managed landscapes to provide roosting habitat. Furthermore, our results suggest that an understanding of sex-specific roost-site selection is critical for developing comprehensive guidelines for creating and maintaining habitat features beneficial to forest bats.     

Reconsidering the importance of harvested forests for the conservation of tree-dwelling bats

Intensively managed forests are often seen as of low priority to preserve forest bats. The main conservation strategy recommended, i.e. saving unmanaged “habitat islands” from logging to preserve some suitable habitat, detracts conservationists’ attention from ameliorating conditions for bats in harvested sites. We studied the threatened bat Barbastella barbastellus, mostly roosting in snags, in two beech forests: an unmanaged forest—the main maternity site—and a nearby, periodically logged area. We compared roost availability, roost use, capture rates, food availability and movement between these areas. The managed forest had a greater canopy closure, fewer dead trees, a smaller tree diameter and trees bearing fewer cavities than the unmanaged one. These differences helped explain the larger number of bats recorded in the unmanaged forest, where the sex ratio was skewed towards females. Prey availability was similar in both areas. We radiotracked bats to 49 day roosts. Five individuals caught in the managed area roosted in the unmanaged one at 6.7–8.2 km from the capture site. Few bats roosted in the managed forest, but those doing so proved flexible, using live trees and even rock crevices. Therefore, bats utilise areas in the matrix surrounding optimal roosting sites and sometimes roost there, highlighting the conservation potential of harvested forests. Besides leaving unmanaged patches, at least small numbers of dead trees should be retained in logged areas to favour population expansion and landscape connectivity. Our findings also question the validity of adopting presence records as indicators of forest quality on a site scale.     

Frugivorous Bats Maintain Functional Habitat Connectivity in Agricultural Landscapes but Rely Strongly on Natural Forest Fragments

Anthropogenic changes in land use threaten biodiversity and ecosystem functioning by the conversion of natural habitat into agricultural mosaic landscapes, often with drastic consequences for the associated fauna. The first step in the development of efficient conservation plans is to understand movement of animals through complex habitat mosaics. Therefore, we studied ranging behavior and habitat use in Dermanura watsoni (Phyllostomidae), a frugivorous bat species that is a valuable seed disperser in degraded ecosystems. Radio-tracking of sixteen bats showed that the animals strongly rely on natural forest. Day roosts were exclusively located within mature forest fragments. Selection ratios showed that the bats foraged selectively within the available habitat and positively selected natural forest. However, larger daily ranges were associated with higher use of degraded habitats. Home range geometry and composition of focal foraging areas indicated that wider ranging bats performed directional foraging bouts from natural to degraded forest sites traversing the matrix over distances of up to three hundred meters. This behavior demonstrates the potential of frugivorous bats to functionally connect fragmented areas by providing ecosystem services between natural and degraded sites, and highlights the need for conservation of natural habitat patches within agricultural landscapes that meet the roosting requirements of bats.     

Abundance responses of frugivorous bats (Stenodermatinae) to coffee cultivation and selective logging practices in mountainous central Veracruz,Mexico

Shifting and permanent cultivation, selective logging, cattle production and coffee plantations are among the most important factors in montane cloud forest conversion and disturbance. Although shaded-coffee plantations can contribute to the preservation of local species richness, abundance of organisms could be determined by habitat resource availability in agricultural landscapes. We compared abundance of Sturnira and Artibeus bats (Phyllostomidae, Stenodermatinae), in shade coffee plantations and disturbed cloud forest fragments, which represent habitats with different chiropterochorous plant density. We also investigated the relationship between bat species abundance and food plant richness, abundance and diversity. We captured 956 bats, 76% in cloud forest fragments and 24% in shaded coffee plantations. Abundance of Sturnira spp. (small bats) was greater in cloud forest than in coffee plantations, but Artibeus spp. (large bats) abundance was similar in both habitats. Chiropterochorous plant abundance was positively related with bat abundance for Sturnira spp., while chiropterochorous plant richness and diversity were negatively related for Artibeus spp. This suggests that frugivorous bats with different morphological and ecological characteristics respond differentially to anthropogenic activities. For landscape management purposes, the maintenance and augmentation of diverse food resources, for frugivorous bats with different foraging requirements in coffee plantations, will benefit the resilience of bats to modification of their natural habitat.     

The effect of bark beetle infestation and salvage logging on bat activity in a national park

Salvage logging—the removal of dead trees in disturbed forest stands—has been controversially discussed. We investigated the impact of bark beetle attacks and subsequent salvage logging on insectivorous bats in a temperate mountain forest. We quantified bat activity (25,373?min counts; 32 plots) using batcorders during 221 all-night surveys in stands killed by bark beetles, with dead trees removed or not, and in vital, single- or multi-layered mature forest stands. We analysed the differences in activity of all bats in general and of bats of foraging guilds (open habitat, forest edge, closed habitat) in these habitats using a generalized linear Poisson mixed model, with plot and observation as random factors, and temperature and habitat as fixed factors. Only open-habitat foragers were slightly more active in salvage-logged stands than in bark-beetle-affected stands; they generally benefited from an open forest canopy, whereas closed-habitat foragers did not. Our results indicated that: (1) bats are less affected by salvage logging after a disturbance of a magnitude typical for European forests, probably because enough roosts are present in surrounding areas, (2) habitats for open foragers are improved by bark beetle infestation and (3) bats are poor bioindicators of negative impacts of salvage logging after natural disturbance in forests with a composition typical for Central Europe.     

EFFECT OF HABITAT AND FORAGING HEIGHT ON BAT ACTIVITY IN THE COASTAL PLAIN OF SOUTH CAROLINA

Abstract: We compared bat activity levels in the Coastal Plain of South Carolina among 5 habitat types: forested riparian areas, clearcuts, young pine plantations, mature pine plantations, and pine savannas. We used time-expansion radio-microphones and integrated detectors to simultaneously monitor bat activity at 3 heights (30, 10, 2 m) in each habitat type. Variation in vegetative clutter among sampling heights and among habitat types allowed us to examine the differential effect of forest vegetation on the spatial activity patterns of clutter-adapted and open-adapted bat species. Moreover, monitoring activity at 30, 10, and 2 m permitted us to also compare bat activity above and below the forest canopy. We detected calls of 5 species or species groups: eastern red/Seminole bats (Lasiurus borealis/L. seminolus), eastern pipistrelles (Pipistrellus subflavus), evening bats (Nycticeius humeralis), big brown bats (Eptesicus fuscus), and hoary bats (Lasiurus cinerius). At 2 and 10 m, bat activity was concentrated in riparian areas, whereas we detected relatively low levels of bat activity in upland habitats at those heights. Activity was more evenly distributed across the landscape at 30 m. Bat activity levels above the forest canopy were almost 3 times greater than within or below the canopy. We detected significantly greater activity levels of 2 open-adapted species (hoary and big brown bats) above rather than within or below the forest canopy. However, activity levels of 2 clutter-adapted species (eastern red/Seminole bats and eastern pipistrelles) did not differ above, within, or below the forest canopy. Despite classification as a clutter-adapted species, evening bat activity was greater above rather than within or below the forest canopy. We believe our results highlight the importance of riparian areas as foraging habitat for bats in pine-dominated landscapes in the southeastern United States. Although acoustical surveys conducted below forest canopies can provide useful information about species composition and relative activity levels of bats that forage in cluttered environments, our results showing activity above canopy suggest that such data may not accurately reflect relative activity of bats adapted to forage in more open conditions, and therefore may provide an inaccurate picture of bat community assemblage and foraging habitat use.     

Use of native woodlands and traditional olive groves by foraging bats on a Mediterranean island: consequences for conservation

We recorded bat activity on Zakynthos island (Greece) to test the hypotheses that (1) olive ( Olea europea ) groves and native woodlands provide comparable foraging habitat for insectivorous bats, (2) lower foraging activity occurs in olive groves treated with insecticide chemicals. We acoustically sampled bat activity (passes per minute) in four wooded habitats (organic and non-organic olive groves, oak woodland ( Quercus ilex and Quercus coccifera ) and pine ( Pinus halepensis ) woodland from June to August 2005. Habitat type did not affect overall bat activity. A single application of insecticide chemicals annually did not affect activity over traditional olive groves. Habitat use on the island differed in several ways from that reported in studies at mainland sites. Most strikingly, pine woodland supported higher bat activity than expected relative to other habitat types, and we recorded unexpectedly high levels of M. capaccinii activity in woodland habitats. We suggest that traditional olive groves buffer some bat species from the effects of deforestation. Conservation plans for Mediterranean bats should consider the biodiversity value of these groves along with the need to conserve small woodland patches. Finally, understanding island-specific patterns of habitat use is essential to bat conservation on small off-shore islands.     

Ensemble composition and activity levels of insectivorous bats in response to management intensification in coffee agroforestry systems

Shade coffee plantations have received attention for their role in biodiversity conservation. Bats are among the most diverse mammalian taxa in these systems; however, previous studies of bats in coffee plantations have focused on the largely herbivorous leaf-nosed bats (Phyllostomidae). In contrast, we have virtually no information on how ensembles of aerial insectivorous bats--nearly half the Neotropical bat species--change in response to habitat modification. To evaluate the effects of agroecosystem management on insectivorous bats, we studied their diversity and activity in southern Chiapas, Mexico, a landscape dominated by coffee agroforestry. We used acoustic monitoring and live captures to characterize the insectivorous bat ensemble in forest fragments and coffee plantations differing in the structural and taxonomic complexity of shade trees. We captured bats of 12 non-phyllostomid species; acoustic monitoring revealed the presence of at least 12 more species of aerial insectivores. Richness of forest bats was the same across all land-use types; in contrast, species richness of open-space bats increased in low shade, intensively managed coffee plantations. Conversely, only forest bats demonstrated significant differences in ensemble structure (as measured by similarity indices) across land-use types. Both overall activity and feeding activity of forest bats declined significantly with increasing management intensity, while the overall activity, but not feeding activity, of open-space bats increased. We conclude that diverse shade coffee plantations in our study area serve as valuable foraging and commuting habitat for aerial insectivorous bats, and several species also commute through or forage in low shade coffee monocultures.     

Poor effectiveness of Natura 2000 beech forests in protecting forest-dwelling bats

With the establishment of the Natura 2000 (N2000) network, the European Union intends to develop strategies to conserve Europe's threatened habitats and species, including bats. Forest-dwelling bats are highly reliant on forest structures, such as snags and hollow trees, which the bats need as roosts. The decrease in such forest microhabitats significantly affects the habitat use and, therefore, the activity in forests. To determine whether N2000 beech forests under active timber production offer better habitats for bats compared to commercially used non-N2000 forests, we measured the bat activity and assessed the potential roosts in trees and snags in eleven pairs of stands. All survey stands represented mesotrophic beech forests (Fagus sylvatica L.) of the N2000 habitat type 9130 (Asperulo-Fagetum) in three European Biogeographic Regions. The activity of all bat species, the activity of priority N2000 species, the species number, the number of trees with roosts and the snag volume did not differ significantly between the N2000 and non-N2000 stands. We conclude that the current management of the N2000 beech forests is almost identical to that of non-N2000 commercial forests, and thus, the N2000 status has not led to an increase of bat-relevant habitat variables yet. Consequently, additional efforts beyond the administrative assignment of N2000 areas are required to build and ensure an ecologically effective and sustainable network of beech forests in Europe, including increasing important forest requirements for bats, such as roosts and snags.     

Habitat aridity and urine concentrating ability of nearctic, insectivorous bats

Urine concentrating ability of nearctic, insectivorous bats determined from renal anatomy was significantly correlated to total annual precipitation and potential evapotranspiration to precipitation ratio of the habitat where the animal was collected. Habitat aridity measured by water input accounted for as much variation in urine concentrating ability as habitat aridity measured by both water and energy input. Habitat aridity, regardless of how it was measured, explained only 25% of the among-species variation in urine concentrating ability. Factors other than habitat aridity significantly affect the urine concentrating ability of insectivorous bats.     

Habitat fragmentation and the prevalence of parasites (Diptera,Streblidae) on three Phyllostomid bat species

Ectoparasitism in bats seems to be influenced strongly by the type of roost preferred by the hosts, and group size; however, the effect of habitat loss and fragmentation on the prevalence of ectoparasites in bats has scarcely been studied. In northeastern Yucatan, Mexico, we estimated the prevalence of infestation by Streblidae flies in three phyllostomid bat species with different roost preferences (caves, trees, or both) in two types of landscape matrices (tropical semi‐deciduous forest and man‐made pastures) that differed in area of forest cover and the number of forest fragments. Habitat fragmentation and the presence of a contrasting matrix may limit the availability of roosts (trees) and the movement of bats across the landscape. Accordingly, we hypothesized higher prevalence of Streblidae infestation in the pasture matrix and in the group of bats that roost in trees. Bat abundance was higher in the pasture matrix; however, the prevalence of infestation was significantly higher in the continuous forest matrix and in bats that roosted in caves. The prevalence of some species of Streblidae was affected by habitat fragmentation in species that roost in caves, such as Desmodus rotundus, as well as those using foliage and caves, such as Artibeus jamaicensis. Our results provide evidence that some species of Streblidae may respond differently to habitat fragmentation than their hosts, generating changes to bat‐ectoparasite interactions in fragmented areas. Environmental variations involving roosts, not evaluated in this study, may influence our results, since these factors affect ectoparasite abundance and reproduction.     

Use of foraging habitats by bats in a Mediterranean area determined by acoustic surveys: conservation implications

We determined habitat use by foraging bats by broad-band acoustic surveys in 10 habitat types from a Mediterranean area (southern Italy). We applied discriminant functions to identify time-expanded echolocation calls from free-flying bats.
Moon phase and cloud cover had no effect on bat activity. Only Hypsugo savii was influenced by temperature, and activity of Myotis daubentonii and Myotis capaccinii was reduced at higher wind speeds. Both total numbers of bat passes and feeding buzzes were highest over rivers and lakes. Pipistrellus kuhlii and H. savii were most frequently recorded. Pipistrellus kuhlii , Pipistrellus pipistrellus and Tadarida teniotis proved generalists in using foraging habitats.
Water sites and conifer plantations were respectively the most and the least used habitats by H. savii . Rivers were especially important to Myotis bats, Miniopterus schreibersii and Pipistrellus pygmaeus . Unlike P. kuhlii , P. pipistrellus was frequent in beech woodlands; P. pygmaeus made a considerable use of chestnut woodlands and Myotis spp. were moderately active in both these woodland types.
A large number of endangered or vulnerable species featured in riparian habitats, broadleaved woodlands and olive groves. Riparian and woodland habitats constitute an important target for conservation. Typical land use forms such as woodlands used for chestnut production and traditionally managed olive groves should be encouraged in conservation plans. The negative impact of urbanisation on bats might be counteracted by fostering trees, gardens and small cultivated patches. Farmland practices should encourage landscape complexity and limit the use of pesticides.     

Bat Response to Differing Fire Severity in Mixed-Conifer Forest California,USA

Wildlife response to natural disturbances such as fire is of conservation concern to managers, policy makers, and scientists, yet information is scant beyond a few well-studied groups (e.g., birds, small mammals). We examined the effects of wildfire severity on bats, a taxon of high conservation concern, at both the stand (<1 ha) and landscape scale in response to the 2002 McNally fire in the Sierra Nevada region of California, USA. One year after fire, we conducted surveys of echolocation activity at 14 survey locations, stratified in riparian and upland habitat, in mixed-conifer forest habitats spanning three levels of burn severity: unburned, moderate, and high. Bat activity in burned areas was either equivalent or higher than in unburned stands for all six phonic groups measured, with four groups having significantly greater activity in at least one burn severity level. Evidence of differentiation between fire severities was observed with some Myotis species having higher levels of activity in stands of high-severity burn. Larger-bodied bats, typically adapted to more open habitat, showed no response to fire. We found differential use of riparian and upland habitats among the phonic groups, yet no interaction of habitat type by fire severity was found. Extent of high-severity fire damage in the landscape had no effect on activity of bats in unburned sites suggesting no landscape effect of fire on foraging site selection and emphasizing stand-scale conditions driving bat activity. Results from this fire in mixed-conifer forests of California suggest that bats are resilient to landscape-scale fire and that some species are preferentially selecting burned areas for foraging, perhaps facilitated by reduced clutter and increased post-fire availability of prey and roosts.     

Larger trees may support larger Indiana bat maternity colonies in a dynamic landscape

Indiana bats (Myotis sodalis), federally listed as endangered, are of management concern in eastern North America. While researchers quantified the habitat affinities of the species throughout the range, few studies have occurred in regions where populations are at high risk for wind energy development and changing climes. Central Illinois, USA, is a dynamic landscape where forest area has been increasing in recent decades (on public and private land) because of changing farming practices and increased habitat protections. The increasing availability of large diameter trees, increasing forest biomass, and changing forest compositions have the potential to influence Indiana bat roost habitat preferences. We assessed Indiana bat maternity roost selection at the tree and forest plot scale to characterize patterns of use in this region from 2017–2018. We predicted that large trees on the landscape would support large colonies of Indiana bats. We located bats in multiple species of trees including elm (Ulmus spp.), cottonwood (Populus deltoides), and shagbark hickory (Carya ovata). We documented larger maternity colonies sharing roosts than in previous studies from the 1980s in the same region. We suggest managers and regulatory agencies monitor Indiana bats in dynamic landscapes such as those with changing forest composition and biomass.