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通过诱变得到十一株木素过氧化物酶酶活降低的黄孢原毛平革菌(Phanerochaetechrysosporium)突变株,用灰色理论分析了其木素过氧化物酶类的产生与木素降解能力间的相关性,并从中筛选到一株木素过氧化物酶缺陷、锰过氧化物酶酶活明显降低的突变株,其木素降解能力为原始菌株的80%左右。该菌粗酶液作用于纤维素酶酶解杉木木素和天然褐腐木素,可产生小分子的木素降解产物,此反应不需H2O2参与。红外光谱分析表明粗酶液对木素的作用主要为氧化作用,因此推测此突变株粗酶液中含有不同于木素过氧化物酶和锰过氧化物酶的与木素氧化降解有关的酶类 相似文献
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黄孢原毛平革菌木素过氧化物酶类在天然木素降解中作用的研究 总被引:12,自引:0,他引:12
通过诱变得到十一株木素过氧化物酶酶知降低的黄孢原毛平革菌突变株,用磷以理论分析了其木素过氧化物酶类的产生与木素降妥能力间的相关性,并从中筛选到一株木素过氧化物酶缺陷,锰过氧化物酶酶活明显降低的突变株,其木素降解能力为原始菌株的80%左右。 相似文献
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木质素过氧化物酶基因5′端上游调控序列的分析 总被引:5,自引:0,他引:5
黄孢原毛平革菌(Phanerochaete chrysosporium)能产生降解木质的胞外木质过氧化物酶(LIP)和锰过氧化物酶(MnP)同工酶。为研究LIP基因的转录调控机理,对LIP基因(GLG3和GLG6)的5′端上游序列进行亚克隆,获得6个亚克隆DNA片段,然后应用凝胶迁移率变动分析技术筛选能与菌体蛋白质专一性结合的DNA片段。结果表明:LIP基因GLG6的5′端上游有一个约670bp的 相似文献
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黄孢原毛平革菌生产锰过氧化物酶的发酵条件研究 总被引:2,自引:0,他引:2
目的 :研究黄孢原毛平革菌产锰过氧化物酶的发酵条件。方法 :对培养条件进行优化 ,采用正交设计法对培养基组分进行优化。结果与结论 :优化培养条件为 :接种量 1 6× 10 6 个孢子 L ,pH 4 4~ 4 8,温度 36℃~ 4 0℃ ,转数 12 0r/min。优化培养基参数为 :葡萄糖 5g L ,酒石酸铵 1 3mmol L ,吐温 - 80 1 2g L ,Mn2 + 0 9mmol L。 相似文献
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Degradation of chlorophenols by P. chrysosporium in static cultures has been studied. The influences of mycelium acclimation, co-substrate concentration and nitrogen source on phenol degradation were analyzed. With non-acclimated mycelium the maximal concentrations degraded were 150 ppm of o-chorophenol and 100 ppm of the isomers m- and p-chlorophenol. The substituted ortho-position on the aromatic ring was the preferred attack position. Meta- and para-positions were less reactive and resulted in a slower degradation rate than the ortho position. Nevertheless, with acclimated mycelium, an increase in the ability to degrade chlorophenol and a higher reactivity in meta- and para-positions were observed (degraded chlorophenol increased by up to 70% for the o-isomer and 50% for the m- and p-isomers with respect to non-acclimated mycelium). A decrease in glucose concentration caused a decrease in chlorophenol degradation rate. Twelve days were needed for complete degradation of o-chlorophenol with 10 g/l of glucose and 22 days when glucose concentration was decreased to 2.5 g/l. The reduction of ammonium tartrate caused a greater lag time, but not a decrease in chlorophenol degradation rate. Replacement of ammonium tartrate by ammonium chloride caused a decrease in chlorophenol degradation rate. 相似文献
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Lidy E. Fratila-Apachitei Jennifer A. Hirst Maarten A. Siebel Huub J. Gijzen 《Biotechnology letters》1999,21(2):147-154
When incubated in synthetic (N-limited) medium and on ashwood chips, Phanerochaete chrysosporium BKM-F-1767 degraded 14 and 10 mg/l diuron, respectively. The wood chips were used as support and sole nutrient source for the fungus. A higher degradation efficiency was found in ashwood culture as compared to the liquid culture, probably as a result of the synergetic effect of attached fungal growth, presence of limiting-substrate conditions and the microenvironment provided by ashwood, all favorable for production of high extracellular enzyme titres. Diuron degradation occured during the idiophasic growth, in the presence of manganese peroxidase, detected as dominant enzyme in both cultures. 相似文献
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本文主要研究利用超声波提取叶绿素。并且对超声提取法和单纯浸泡法作了比较,同时,分析了叶绿素提取效率对时间的依赖性。实验结果显示,用超声来提取绿色植物中的叶绿素,可以增大提取率,缩短提取时间。 相似文献
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Abstract Four major hemoproteins were purified by isoelectric focusing from an extracellular crude enzyme preparation, produced by the white rot fungus Phanerochaete chrysosporium under carbon-limited conditions. Both the crude enzyme and the purified proteins oxidised milled wood lignin, HCl-dioxane-extracted straw lignin and alkali straw lignin in the presence of hydrogen peroxide. The oxidation resulted mainly in further polymerisation of the lignins and was enhanced by addition of veratryl alcohol to the reaction mixture. Alkali straw lignin was also polymerised by horseradish peroxidase, although veratryl alcohol had no influence on this reaction. 相似文献
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Bonnarme P Delattre M Drouet H Corrieu G Asther M 《Biotechnology and bioengineering》1993,41(4):440-450
Phanerochaete chrysosporium and cultivated both mechanically agitated and pneumatic bioreactors. In the pneumatic devices, the yields of lignin and manganese peroxidases as well as extracellular protein, were considerably increased as compared with mechanically agitated bioreactors. Lignin peroxidase and manganese peroxidase activities as high as 4500 U . L(-1) and 1812 U . L(-1) respectively, were produced in an airlift bioreactor. By using enzyme markers, the secretion pathway and the respiration were shown to be dramatically activated in pneumatic bioreactors. The general metabolism of the fungus, when cultivated in the conventional fermentors, is oriented toward the synthesis of biomass at the expense of the synthesis of peroxidases. The use of pneumatic devices for the production of extracellular peroxidases by P. chrysosporium, avoids shear effects due to turbine agitator in the conventional fermentors, and provides a good example for the production of shear-sensitive metabolites. (c) 1993 John Wiley & Sons, Inc. 相似文献