抗癌新药K－AM的合成

本文以鼠李糖和对氨基苯甲酸为原料合成了抗癌新药Ｋ－ＡＭ,并作了红外鉴定。     

糖皮质激素快速抑制高钾离子诱导嗜铬细胞瘤细胞内游离钙浓度升高 …

本研究应用钙离子特异光指示剂Ｆｕｒａ－２／ＡＭ,使用Ｍｉｒａｃａｌ影像系统检测了糖皮质激素对高钾离子升高嗜铬细胞瘤细胞（ＰＣ１２细胞）内游离钙浓度（［Ｃａ＾＋］ｉ）作用的影响。结果表明：（１）皮质酮抑制高钾离子诱导ＰＣ１２细胞［Ｃａ＾２＋］ｉ升高与其预处理细胞时间的长短有关,预处理３ｍｉｎ时,皮质酮开始产生抑制作用;预处理５ｍｉｎ时,其呈现的抑制作用最;预处理２５ｍｉｎ时,抑制作用基本消失。（２）     

Hydrogen peroxide－induced changes in intracellular pH of guard cells precede stomatal closure

INTRODUCTIONEven under optimal conditions, many metabolicprocesses, including chloroplastic, mitochondrial,and plasma membrane-linked electron transportsystems, produce reactive oxygen species (ROS)such as the superoxide radical (OZ--), hydrogenperoxide (HZOZ), and the hydroxyl free radical(OH--)[1, 2]. Furthermore, the imposition of bioticand abiotic stress conditions can give rise to ex-cess concentrations of ROS, resulting in oxidativedamage at the cellular level. Interestingly, R…     

人促血小板生成素受体c—Mpl编码区全长cDNA的克隆及其稳定？…

以人ＨＥＬ细胞总ＲＮＡ为模板,采用ＲＴ－ＰＣＲ方法扩增了人促血小板生成素受体ｃ－Ｍｐｌ编码区全长１．９ｋｂ ｃＤＮＡ,测序结果表明与已报道的序列一致。然后构建了ｃ＝ｍｐｌ的ｐｃＤＮＡ３表达载体ｐｃＭＰＬ,转染不表达ｃｍｐｌ的Ｋ５６２细胞后,经Ｇ４１８抗性筛选,Ｎｏｒｔｈｅｒｎ ｂｌｏｔ和Ｓｏｕｔｈｅｒｎ ｌｂｏｔ检测证实获得稳定表达ｃ－ｍｐｌ的细胞株。为进一步研究ｃ－Ｍｐｌ的生物学功能提供有用的实     

磷脂酰肌醇—4—激酶的免疫分析

在化猪肝微粒体磷脂酰肌醇－４－激酶（ＰＩ－４－Ｋ）的基础上,制备该酶的免疫血清,提纯ＩｇＧ,并建立了ＰＩ４－Ｋ的ＥＬＩＳＡ,进行不同组织中ＰＩ４－Ｋ的免疫沉淀分析和免疫定量。结果证明：猪肝微粒体ＰＩ４－Ｋ的抗血清或抗体ＩｇＧ可沉淀猪肾和猪肺的微粒膜,猪肝细胞膜和人中性粒细胞细胞膜ＴｒｉｔｏｎＸ－１００增溶液（ＴＸＳＭ）中的ＰＩ４－Ｋ表明不同组织,不同亚细胞和不同种属的ＰＩ４－Ｋ有相似的抗原性。猪肝     

百合花粉细胞中钙离子的荧光测定法

以川百合花粉为材料,利用孵育法在花粉原生质体中,特别是利用低温装载法在完整花粉粒中,成功地载入酯化形成的钙离子荧光探针fluo-3AM,利用激光共聚焦显微技术对花粉细胞质中游离钙离子的分布特点进行了研究的结果表明,花粉萌发初期细胞质内游离钙离子呈极性分布,萌发沟附近的钙离子浓度明显高于其他部位,萌发孔附近最高,花粉细胞核中较低,文中对低温装载法的可行性进行了讨论。     

低温处理对冬、春小麦细胞Ca^2＋时空变化的影响

用Fluo-3/AM染色,通过激光扫描共聚焦显微镜（LSCM）方法,对静息态及连续降温条件下不同抗寒性小麦（Triticum aestivum L.）叶肉细胞原生质体[Ca^2 ]cyt（the free Ca^2 concentration in the cytoplasm）的时空变化进行了比较。结果表明,静息态下小麦原生质体整体荧光强度基本不变,暗示[Ca^2 ]cyt能维持在一稳定水平;同时,不同品种小麦间也显示了[Ca^2 ]cyt水平荧光强度的不同。温度由15℃连续降至约2℃时,抗寒冬小麦[Ca^2 ]cyt出现升高后的回复,2℃之后逐渐升高;冷敏感春小麦则无此回复过程,而是一直升高到最大值。推测这一不同的动态变化最终决定了植物在低温下产生冷适应的不同能力。这进一步为“Ca^2 是低温下生理信号的传导”这一假说提供了新的证据。     

钙结合蛋白D-9K基因原核表达载体的构建、表达产物纯化及其多克隆抗体的制备

本研究构建了pBV220/CabpD-9k-βhCG表达质粒,在大肠杆菌TG-1中进行表达,得到了CabpD-9k-βhCG融合蛋白。SDS-PAGE分析显示融合蛋白的分子量约为34.5KD。Western blot结果表明,融合蛋白与兔抗人hCGIgG特异结合。用纯化的融合蛋白免疫C57BL小鼠,制备了多克隆抗体,这为进一步研究钙结合蛋白D-9K在着床中的功能和子宫内膜Ca2+信号转导奠定了基础。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.