共查询到20条相似文献,搜索用时 0 毫秒
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Urm1 (ubiquitin-related modifier), involved in diverse biological processes in yeast, is proved to be a “molecular fossil”
in ubiquitin superfamily. Here we report the resonance assignment of Urm1 from Trypanosoma brucei. 相似文献
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SUMO (small ubiquitin-like modifier) plays important roles in diverse processes by posttranslationally modifying many proteins.
Here we report the resonance assignment of the truncated SUMO from Trypanosoma brucei. 相似文献
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Trijntje J. Pool Nur Alia Oktaviani Hironari Kamikubo Mikio Kataoka Frans A. A. Mulder 《Biomolecular NMR assignments》2013,7(1):97-100
Photoactive yellow protein (PYP) is involved in the negative phototactic response towards blue light of the bacterium Halorhodospira halophila. Here, we report nearly complete backbone and side chain 1H, 13C and 15N resonance assignments at pH 5.8 and 20 °C of PYP in its electronic ground state. 相似文献
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Marc Quinternet Laure Selme Chrystel Beaufils Pascale Tsan Christophe Jacob Sandrine Boschi-Muller Marie-Christine Averlant-Petit Guy Branlant Manh-Thong Cung 《Biomolecular NMR assignments》2008,2(1):85-87
We report the nearly complete 1H, 13C, and 15N resonance assignments of the C103S mutant of the N-terminal domain of DsbD from Neisseria meningitides. Secondary structure determination using CSI method leads to the prediction of nine β-sheet parts. 相似文献
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Veronica Esposito Valeria Musi Daniele Veggi Annalisa Pastore Mariagrazia Pizza 《Biomolecular NMR assignments》2010,4(1):107-109
GNA2132 (Genome-derived Neisseria Antigen 2132) is a surface-exposed lipoprotein discovered by reverse vaccinology and expressed by genetically diverse Neisseria meningitidis strains (Pizza et al. 2000). The protein induces bactericidal antibodies against most strains of Meningococccus and has been included in a multivalent recombinant vaccine against N. meningitidis serogroup B. Structure determination of GNA2132 is important for understanding the antigenic properties of the protein in view of increased efficiency vaccine development. We report practically complete 1H, 13C and 15N assignment of the detectable spectrum of a highly conserved C-terminal region of GNA2132 (residues 245–427) in micellar solution, a medium used to improve the spectral quality. The first 32 residues of our construct up to residue 277 were not visible in the spectrum, presumably because of line broadening due to solvent and/or conformational exchange. Secondary structure predictions based on chemical shift information indicate the presence of an all β-protein with eight β strands. 相似文献
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Neumoin A Leonchiks A Petit P Vuillard L Pizza M Soriani M Boelens R Bonvin AM 《Biomolecular NMR assignments》2011,5(2):135-138
GNA1946 (Genome-derived Neisseria Antigen 1946) is a highly conserved exposed outer membrane lipoprotein from Neisseria meningitidis bacteria of 287 amino acid length (31 kDa). Although the structure of NMB1946 has been solved recently by X-Ray crystallography,
understanding the behaviour of GNA1946 in aqueuos solution is highly relevant for the discovery of the antigenic determinants
of the protein that will possibly lead to a more efficient vaccine development against virulent serogroup B strain of N.meningitidis. Here we report almost complete 1H, 13C and 15N resonance assignments of GNA1946 (residues 10–287) in aqueous buffer solution. 相似文献
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One of the small proteins from Helicobacter pylori, HP1242, was investigated by the solution nuclear magnetic resonance (NMR) spectroscopy. HP1242 is known as a 76-residue conserved hypothetical protein and its function cannot be identified based on sequence homology. Here, the results of the backbone (1)H, (15)N, and (13)C resonance assignments of the HP1242 are reported using double- and triple-resonance techniques. About 95 % of all of the (1)HN, (15)N, (13)CO, (13)Calpha, and (13)Cbeta resonances that cover 75 non-Proline residues of the 76 residues are clarified through sequential- and specific- assignments. In addition, three helical regions were clearly identified on the basis of the resonance assignments. 相似文献
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Tony Chen Daoning Zhang Yulia Matiuhin Michael Glickman David Fushman 《Biomolecular NMR assignments》2008,2(2):147-149
The ubiquitin-like domain (UBL) of yeast protein Dsk2p is widely believed to recognize and bind to ubiquitin receptors on
the proteasome and, as part of Dsk2p, to bridge polyubiquitinated substrates and proteasomal degradation machinery. Here we
report NMR resonance assignment for 1H, 15N, and 13C nuclei in the backbone and side chains of the UBL domain of Dsk2p. This assignment will aid in NMR studies focused on understanding
of Dsk2’s interactions with proteasomal receptors and its role as a polyubiquitin shuttle in the ubiquitin-dependent proteasomal
degradation as well as other cellular pathways. 相似文献
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HtrA is a major virulence factor of Streptococcus pneumoniae (pneumococcus). HtrA is involved in the ability of the pneumococcus to grow at high temperature, to resist oxidative stress and control the bacteriocin activity. Here we report the resonance assignment of the PDZ domain of HtrA from Streptococcus pneumoniae TIGR4. 相似文献
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The 18.8 kDa chitin-binding protein CBP21 from Serratia marcescens has been isotopically labeled and recombinantly expressed. In this paper, we report the 1H, 13C, 15N resonance assignment of CBP21. 相似文献
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RegB is involved in the control of the phage T4 life cycle. It inactivates the phage early mRNAs when their translation is
no more required. We determined its structure and identified residues involved in substrate binding. For this, all backbone
and 90% of side-chain resonance frequencies were assigned. 相似文献
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Fabio Baroni Luigia Pazzagli Simone Luti Aniello Scala Federica Martellini Lorella Franzoni Thelma A. Pertinhez Alberto Spisni 《Biomolecular NMR assignments》2014,8(2):405-408
Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered. 相似文献
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Tsuruta T Goda N Umetsu Y Iwaya N Kuwahara Y Hiroaki H 《Biomolecular NMR assignments》2012,6(1):23-25
Stomatin, a 288-residue protein, is a component of the membrane skeleton of red blood cells (RBCs), which helps to physically
support the membrane and maintains its function. In RBCs, stomatin binds to the glucose transporter GLUT-1 and may regulate
its function. Stomatin has a stomatin/prohibitin/flotillin/HflK (SPFH) domain at the center of its polypeptide chain. There
are 12 SPFH domain-containing proteins, most of which are localized at the cellular or subcellular membranes. Although the
molecular function of the SPFH domain has not yet been established, the domain may be involved in protein oligomerization.
The SPFH domain of the archaeal stomatin homolog has been shown to form unique oligomers. Here we report the 15N, 13C, and 1H chemical shift assignments of the SPFH domain of human stomatin [hSTOM(SPFH)]. These may help in determining the structure
of hSTOM(SPFH) in solution as well as in clarifying its involvement in protein oligomerization. 相似文献
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Melanie Schwarten Matthias Stoldt Jeannine Mohrlüder Dieter Willbold 《Biomolecular NMR assignments》2009,3(1):137-139
The autophagy-related protein Atg8 is important for the formation of autophagosomes as it mediates membrane fusion. To elucidate
the solution structure of Atg8 backbone and side chain chemical shifts of Atg8 were assigned as far as possible. 相似文献
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Uybach Vo Kevin J. Embrey Alexander L. Breeze Alexander P. Golovanov 《Biomolecular NMR assignments》2013,7(2):215-219
K-Ras, a member of the Ras family of small GTPases, is involved in cell growth, proliferation, differentiation and apoptosis and is frequently mutated in cancer. The activity of Ras is mediated by the inter-conversion between GTP- and GDP- bound states. This conversion is regulated by binding of effector proteins such as guanine nucleotide exchange factors and GTPase activating proteins. Previously, NMR signals from these effector-binding regions of Ras often remained unassigned and largely unobservable due to conformational exchange and polysterism inherent to this protein. In this paper, we report the complete backbone and Cβ, as well as partial Hα, Hβ and Cγ, NMR assignment for human K-Ras (residues 1–166) in the GDP-bound form at a physiological pH of 7.4. These data thereby make possible detailed monitoring of the functional cycle of Ras and its interactions with nucleotides and effector proteins through the observation of fingerprint signals from all the functionally important regions of the protein. 相似文献
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Umetsu Y Goda N Taniguchi R Satomura K Ikegami T Furuse M Hiroaki H 《Biomolecular NMR assignments》2011,5(2):207-210
Zonula occludens-1 (ZO-1) is a scaffolding molecule critical to the formation of intercellular adhesion structures, such as
tight junctions (TJs) and adherens junctions (AJs). ZO-1 contains three PDZ domains followed by a GUK domain and a ZU5 domain.
The first PDZ of ZO-1 (ZO-1(PDZ1)) serves as a protein–protein interaction module and interacts with the C-termini of almost
all claudins to initiate the formation of a belt-like structure on the lateral membranes, thereby promoting TJ formation.
It has been recently reported that approximately 15% of all PDZ domains bind phosphoinositides, and ZO-1(PDZ1) is the one
of these. Here we report the 15N, 13C, and 1H chemical shift assignments of the first PDZ domain of mouse ZO-1. The resonance assignments obtained in this work may contribute
in clarifying the interplay between the two binary interactions, ZO-1(PDZ1)–claudins and ZO-1(PDZ1)–phospholipids, and suggesting
a novel regulation mechanism underlying the formation and maintenance of cell–cell adhesion machinery downstream of the phospholipid
signaling pathways. 相似文献