共查询到20条相似文献,搜索用时 31 毫秒
1.
Clerosterol-[26-14C], a 24β-ethyl-25-methylene sterol [(24S)-24-ethylcholesta-5,25-dien-3β-ol], was incorporated into clionasterol and poriferasterol by cultures of the green algae Trebouxia sp. 213/3 and Trebouxia sp. 219/2. Degradation of the labelled poriferasterol showed that the 14C retained its identity and was not incorporated as a result of metabolism of the clerosterol-[26-14C] and randomisation of label. These results are consistent with the proposed production, and subsequent reduction, of a 24β-ethyl-25-methylene intermediate in 24β-ethyl sterol biosynthesis in algae of the order Chlorococcales. 相似文献
2.
《Biochimica et Biophysica Acta (BBA)/General Subjects》1987,923(2):196-205
[7-14C]2-Ethyl-5-carboxypentyl phthalate was isolated and purified from urine of rats given [7-14C]-di-(2-ethylhexyl) phthalate. This metabolite was shown to serve as a precursor for 2-ethyl-3-carboxypropylphthalate in vivo. 2-Ethyl-5-carboxypentyl phthalate was oxidized to 2-ethyl-3-carboxypropyl phthalate in liver slices from control or, much more rapidly, from clofibrate-pretreated rats. Inhibition by KCN in liver slices from untreated rats, and strong inhibition by acrylate, suggested that formation of 2-ethyl-3-carboxy-propyl phthalate involved mitochondria β-oxidation. The strong enhancement of the product of this compound by clofibrate (a very weak inducer for mitochondrial dehydrogenases), and strong inhibition by chlorpromazine suggested that peroxisomes may also be able to oxidize 2-ethyl-5-carboxypentyl phthalate. We were able to detect β-oxidation of 2-ethyl-5-carboxypentyl phthalate to 2-ethyl-3-carboxypropyl phthalate using purified mitochondria, but strong phthalate monoester hydrolase activity observed during incubation of the former compound with purified peroxisomes made it impossible to determine whether 2-ethyl-3-carboxypropyl phthalate could be produced in the latter organelle or not. 2-Ethyl-5-carboxypentyl phthalate was such an inefficient substrate for β-oxidation compared to palmitic acid that it is unlikely that it contributes significantly to the production of H2O2 in rats chronically exposed to di-(2-ethylhexyl) phthalate. Normal fatty acids are most likely to serve as the dominat substrates for peroxisomal β-oxidase. 相似文献
3.
The structures of two 4α-methylsterols is isolated from Cucumis sativus(Cucurbitaceae) seeds were determined based mainly on their 13CNMR spectra as 24β-ethyl-31-norlanosta-8,25(27)-dien-3β-ol and 24β-ethyl-25(27)- dehydrolophenol, respectively, of which the former is a new sterol from natural sources. These two 4α-methylsterols were identified in the seeds of two other Cucurbitaceae species, Lagenaria leucantha var. Gourda and Citrullus battich. The probable biogenetic significance of the two 4α-methylsterols is discussed. Other 4α-methylsterols identified in the seeds of the three Cucurbitaceae species were obtusifoliol, cycloeucalenol and gramisterol. 相似文献
4.
Jacek W. Morzycki Zenon ?otowski Piotr Wa?ejko Jan Kowalski Andrzej Sobkowiak 《Carbohydrate research》2010,345(8):1051-1055
A new electrochemical glycosylation method is presented. According to the method cholesterol and other 3β-hydroxy-Δ5-steroids can be selectively transformed to glycosides using non-activated sugars. The method is also useful for the synthesis of glycoconjugates with sugar linked to a steroid moiety by an ether bond. 相似文献
5.
白细胞介素24(interleukin 24,IL-24)是近年来新发现的1个IL-10家族细胞因子,具有明显的抗肿瘤活性.为了研究开发高活性、低分子量的IL-24,并探讨其用于肿瘤靶向治疗的可能性,本研究在前期基础上,进一步构建并制备了缺失N端103个氨基酸残基的IL-24(hIL-24Δ103)重组腺病毒,并观察了其对A549细胞生长增殖和凋亡的影响.首先,采用PCR技术扩增IL-24第104位至第206位氨基酸区域的编码序列,制备hIL-24Δ103重组腺病毒.用Ad-hIL-24Δ103重组腺病毒感染肺癌A549细胞. MTT分析结果表明,Ad-hIL-24Δ103感染显著抑制了A549细胞的生长.Hoechst 33258染色和流式细胞仪分析结果表明,Ad-hIL 24Δ103感染导致细胞凋亡.Western 印迹分析结果表明,Ad-hIL-24Δ103感染导致了PKR和eIF-2α蛋白的表达上调与磷酸化激活,提示PKR和eIF-2α参与了hIL-24Δ103导致的细胞生长抑制和细胞凋亡过程的调节.关键词 人白介素24;腺病毒;细胞增殖;细胞凋亡 相似文献
6.
John W. Huffman John Liddle Sammy G. Duncan Jr. Shu Yu Billy R. Martin Jenny L. Wiley 《Bioorganic & medicinal chemistry》1998,6(12):2383-2396
The synthesis of the 3-heptyl, and the eleven isomeric 3-methylheptyl-Δ8-tetrahydrocannabinols (3–7, R and S methyl epimers, and 8) has been carried out. The synthetic approach entailed the synthesis of substituted resorcinols, which were subjected to acid catalyzed condensation with trans-para-menthadienol to provide the Δ8-THC analogue. The 1′-, 2′- and 3′-methylheptyl analogues (3–5) are considerably more potent than Δ8-THC. The 4′-, 5′- and 6′-methylheptyl isomers (6–8) are approximately equal in potency to Δ8-THC. 相似文献
7.
Malcolm J. Thompson Samson R. Dutky Yolanda Lehner Lonnie N. Standifier Elton W. Herbert 《Phytochemistry》1978,17(6):1053-1056
The esterified and unesterified sterol fractions of bee-gathered mixed pollens were examined, and total sterol composition was determined. Two new sterols of pollens, 14α-methyl-9β,19-cyclo-5α-cholest-24-en-3β-ol (24-dehydropollinastanol) and 14α-methyl-5α-ergost-24(28)-en-3β-ol (24-methylenepollinastanol) were isolated and identified. Both sterols were found primarily in the esterified sterol fraction, and 24-methylenepollinastanol accounted for 43% of the sterols of this fraction. 24-Dehydropollinastanol and four other sterols which also contain a 9β,19-cyclopropane ring were found only in the esterified sterol fraction. 24-Methylenecholesterol was the major sterol of the unesterified sterol fraction. 相似文献
8.
9.
10.
Gioiello A Macchiarulo A Carotti A Filipponi P Costantino G Rizzo G Adorini L Pellicciari R 《Bioorganic & medicinal chemistry》2011,19(8):2650-2658
Within our efforts in the discovery of novel potent and selective ligands for the FXR receptor, 23-N-(carbocinnamyloxy)-3α,7α-dihydroxy-6α-ethyl-24-nor-5β-cholan-23-amine was synthesized and evaluated for its ability to activate and modulate the biological response of the receptor. Alphascreen and RT-PCR revealed that the 6α-ethyl-24-norcholanyl-23-amine derivate behaves as full FXR agonist endowed with high binding affinity and efficacy, representing a promising lead candidate for further optimization. In addition, docking studies provide new insights into the molecular basis governing the partial and full agonist activity at FXR. 相似文献
11.
Sungzong Kang Michael V.W. Bergamini Michael Hasen 《Journal of theoretical biology》1976,63(1):117-124
The conformations of 2S,3R-2-(N-formyl)amino-l,3-dihydroxy-Δ31-pentene, a model compound of sphingomyelin, have been studied both by the classical potential function and by the INDO molecular orbital method. The results suggest that the preferred conformation of sphingomyelin in the membrane is such that the olefinic double bond of the γ-hydrocarbon chain and the planar amide group of the β-chain are parallel and stack in an antiparallel manner with dihedral angles β1′(C3-C2-N21-C21) = ?100 ° and γ1(C2-C3-C31-C32) = ?100 °. 相似文献
12.
Maarten Niemantsverdriet Peter Nagle Roland K. Chiu Johannes A. Langendijk Harm H. Kampinga Robert P. Coppes 《PloS one》2012,7(12)
The p53 homolog p73 is frequently overexpressed in cancers. Especially the transactivation domain truncated isoform ΔNp73 has oncogenic properties and its upregulation is associated with poor patient survival. It has been shown that ΔNp73 has an inhibitory effect on the transactivation capacity of p53 and other p73 isoforms. Here, we confirm this finding but surprisingly find that ΔNp73 may also stimulate the expression of TGF-β signaling targets. Promoter-reporter analysis indicated that the presence of Smad Binding Elements (SBE) in the promoter is sufficient for stimulation of gene expression by ΔNp73. TGF-β signaling was less efficient in ΔNp73 downregulated cells, whereas tetracycline induced ΔNp73 increased expression of endogenous TGF-β regulated genes PAI-1 and Col1a1. Pull-down assays with SBE DNA suggest that ΔNp73 enhances smad3/4 binding to SBEs, thereby stimulating TGF-β signaling. Chromatin immunoprecipitation assays confirmed a direct interaction between ΔNp73 and SBE. Given the role of TGF-β signaling in carcinogenesis, tumor invasion and metastasis via targets like PAI-1 and Col1a1, our data suggest a model on how this effect of ΔNp73 could be a contributing factor in cancer progression. 相似文献
13.
14.
HP Van Kuringen J Lenoir E Adriaens J Bender BG De Geest R Hoogenboom 《Macromolecular bioscience》2012,12(8):1114-1123
The hydrolysis of PEtOx is studied to evaluate the potential toxicity of partially hydrolyzed polymers that might interfere with its increasing popularity for biomedical applications. The hydrolysis of PEtOx is studied in the presence of digestive enzymes (gastric and intestinal) and at 5.8?M hydrochloric acid as a function of temperature (57, 73, 90, and 100?°C). It is found that PEtOx undergoes negligible hydrolysis at 37?°C and that thermal and solution properties are not altered when up to 10% of the polymer backbone is hydrolyzed. Mucosal irritation and cytotoxicity is also absent up to 10% hydrolysis levels. In conclusion, PEtOx will not decompose at physiological conditions, and partial hydrolysis will not limit its biomedical applications. 相似文献
15.
Δ′-Dehydrogenation of steroids byArthrobacter simplex immobilized in calcium polygalacturonate beads
Summary
Arthrobacter simplex ATCC 6946 (viable cells) was immobilized in a calcium polygalacturonate gel. The trapped cells were used for repeated batchwise bioconversion of steroids. Reichstein's compound S and hydrocortisone were dehydrogenated introducing a double bond between C1 and C2 of ring A. The products 1-dehydro S and prednisolone, respectively, were identified by high pressure liquid chromatography. Steroid dehydrogenase activity increased in the system when an artificial electron acceptor, such as menadione (vitamin K3) was present in the reaction mixture. An airlift-type reactor was used to bioconvert up to 90% of substrate in 15 min, under optimal conditions. The gel entrapped cell preparations were used for repeated batch bioconversion during 30 days; 69 batch bioconversions for Reichstein's compound S were performed during 15 days of operation of the reactor. The operational stability of the process and the feasibility of repeated batch bioconversions was shown to be comparable to similar processes. 相似文献
16.
17.
Alain Medawar Thierry Virolle Philippe Rostagno Stéphanie de la Forest-Divonne Karen Gambaro Matthieu Rouleau Daniel Aberdam 《PloS one》2008,3(10)
In vivo studies have demonstrated that p63 plays complex and pivotal roles in pluristratified squamous epithelial development, but its precise function and the nature of the isoform involved remain controversial. Here, we investigate the role of p63 in epithelial differentiation, using an in vitro ES cell model that mimics the early embryonic steps of epidermal development. We show that the ΔNp63 isoform is activated soon after treatment with BMP-4, a morphogen required to commit differentiating ES cells from a neuroectodermal to an ectodermal cell fate. ΔNp63 gene expression remains high during epithelial development. P63 loss of function drastically prevents ectodermal cells to commit to the K5/K14-positive stratified epithelial pathway while gain of function experiments show that ΔNp63 allows this commitment. Interestingly, other epithelial cell fates are not affected, allowing the production of K5/K18-positive epithelial cells. Therefore, our results demonstrate that ΔNp63 may be dispensable for some epithelial differentiation, but is necessary for the commitment of ES cells into K5/K14-positive squamous stratified epithelial cells. 相似文献
18.
Summary The in vitro transformation of pregnenolone into progesterone in Digitalis lanata tissues was shown to be catalyzed by a 3-hydroxysteroid dehydrogenase/ketosteroid isomerase (3-HSD). Product formation was monitored by HPLC. The enzyme could be partially characterized and 3-HSD activities were measured in various Digitalis lanata tissues and in cell cultures of other plant species. Since no correlation was observed between biosynthetic competence of the tissue and 3-HSD activity, it was concluded that this enzyme does not play a major role in regulating cardenolide biosynthesis.Abbreviations BA
benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- DMSO
dimethyl sulfoxide
- DMF
N,N-dimethylformamide
- IAA
indole-3-acetic acid 相似文献
19.
20.
Kristian Otkjaer Helmut Holtmann Tue Wenzel Kragstrup S?ren Riis Paludan Claus Johansen Matthias Gaestel Knud Kragballe Lars Iversen 《PloS one》2010,5(1)