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Aberrant regulation of survivin by the RB/E2F family of proteins 总被引:12,自引:0,他引:12
Jiang Y Saavedra HI Holloway MP Leone G Altura RA 《The Journal of biological chemistry》2004,279(39):40511-40520
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Jessica L. Woolnough Blake L. Atwood Keith E. Giles 《Molecular and cellular biology》2015,35(13):2278-2294
To further our understanding of the RNAi machinery within the human nucleus, we analyzed the chromatin and RNA binding of Argonaute 2 (AGO2) within human cancer cell lines. Our data indicated that AGO2 binds directly to nascent tRNA and 5S rRNA, and to the genomic loci from which these RNAs are transcribed, in a small RNA- and DICER-independent manner. AGO2 chromatin binding was not observed at non-TFIIIC-dependent RNA polymerase III (Pol III) genes or at extra-TFIIIC (ETC) sites, indicating that the interaction is specific for TFIIIC-dependent Pol III genes. A genome-wide analysis indicated that loss of AGO2 caused a global increase in mRNA expression level among genes that flank AGO2-bound tRNA genes. This effect was shown to be distinct from that of the disruption of DICER, DROSHA, or CTCF. We propose that AGO2 binding to tRNA genes has a novel and important regulatory role in human cells. 相似文献