共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
Elena M. Pugacheva Samuel Rivero-Hinojosa Celso A. Espinoza Claudia Fabiola Méndez-Catalá Sungyun Kang Teruhiko Suzuki Natsuki Kosaka-Suzuki Susan Robinson Vijayaraj Nagarajan Zhen Ye Abdelhalim Boukaba John E. J. Rasko Alexander V. Strunnikov Dmitri Loukinov Bing Ren Victor V. Lobanenkov 《Genome biology》2015,16(1)
3.
4.
5.
6.
7.
Kosaka-Suzuki N Suzuki T Pugacheva EM Vostrov AA Morse HC Loukinov D Lobanenkov V 《The Journal of biological chemistry》2011,286(31):27378-27388
8.
9.
10.
11.
The transcriptional regulator network of human inflammatory macrophages is defined by open chromatin
Susanne V Schmidt Wolfgang Krebs Thomas Ulas Jia Xue Kevin Ba?ler Patrick Günther Anna-Lena Hardt Hartmut Schultze Jil Sander Kathrin Klee Heidi Theis Michael Kraut Marc Beyer Joachim L Schultze 《Cell research》2016,26(2):151-170
12.
BAT3 and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression 总被引:1,自引:0,他引:1
Nguyen P Bar-Sela G Sun L Bisht KS Cui H Kohn E Feinberg AP Gius D 《Molecular and cellular biology》2008,28(21):6720-6729
Chromatin status is characterized in part by covalent posttranslational modifications of histones that regulate chromatin dynamics and direct gene expression. BORIS (brother of the regulator of imprinted sites) is an insulator DNA-binding protein that is thought to play a role in chromatin organization and gene expression. BORIS is a cancer-germ line gene; these are genes normally present in male germ cells (testis) that are also expressed in cancer cell lines as well as primary tumors. This work identifies SET1A, an H3K4 methyltransferase, and BAT3, a cochaperone recruiter, as binding partners for BORIS, and these proteins bind to the upstream promoter regions of two well-characterized procarcinogenic genes, Myc and BRCA1. RNA interference (RNAi) knockdown of BAT3, as well as SET1A, decreased Myc and BRCA1 gene expression but did not affect the binding properties of BORIS, but RNAi knockdown of BORIS prevented the assembly of BAT3 and SET1A at the Myc and BRCA1 promoters. Finally, chromatin analysis suggested that BORIS and BAT3 exert their effects on gene expression by recruiting proteins such as SET1A that are linked to changes in H3K4 dimethylation. Thus, we propose that BORIS acts as a platform upon which BAT3 and SET1A assemble and exert effects upon chromatin structure and gene expression. 相似文献
13.
Renaud S Pugacheva EM Delgado MD Braunschweig R Abdullaev Z Loukinov D Benhattar J Lobanenkov V 《Nucleic acids research》2007,35(21):7372-7388
14.
15.
16.
17.
18.
19.
20.
BORIS/CTCFL is a member of cancer testis antigen family normally expressed in germ cells. In tumors, it is aberrantly expressed although its functions are not completely well-defined. To better understand the functions of BORIS in cancer, we selected the embryonic cancer cells as a model. Using a molecular beacon, which specifically targets BORIS mRNA, we demonstrated that BORIS positive cells are a small subpopulation of tumor cells (3–5% of total). The BORIS-positive cells isolated using BORIS-molecular beacon, expressed higher telomerase hTERT, stem cell (NANOG, OCT4, SOX2) and cancer stem cell marker genes (CD44 and ALDH1) compared to the BORIS-negative tumor cells. In order to define the functional role of BORIS, stable BORIS-depleted embryonic cancer cells were generated. BORIS silencing strongly down-regulated the expression of hTERT, stem cell and cancer stem cell marker genes. Moreover, the BORIS knockdown increased cellular senescence in embryonic cancer cells, revealing a putative role of BORIS in the senescence biological program. Our data indicate an association of BORIS expressing cells subpopulation with the expression of stemness genes, highlighting the critical role played by BORIS in embryonic neoplastic disease. 相似文献