共查询到20条相似文献,搜索用时 15 毫秒
1.
Isao Karube Tadashi Matsunaga Satoshi Mitsuda Shuichi Suzuki 《Biotechnology and bioengineering》1977,19(10):1535-1547
Two different types of biochemical oxygen demand (BOD) sensors using microbial electrodes were prepared. First, a microbial electrode using the bacteria–collagen membrane and oxygen electrode was used for the determination of BOD. When the electrode was inserted in a sample solution containing glucose and glutamic acid (model waste water), the current of the electrode decreased markedly with time until a steady state was reached. A linear relationship was observed between the steady state current and the concentration of the standard solution containing glucose–glutamic acid or the BOD of the solution. The BOD of industrial waste waters can be estimated within 15 min by using the microbial electrode. No decrease in current output was observed over a ten day period. The reproducibility was determined using the same sample (10% of the standard solution) and was found to be 26.2 ± 2.0 μA (7.5% of the relative standard deviation). Next, a biofuel cell utilizing microbial electrode (immobilized Clostridium butyricum–platinum electrode) was applied to the estimation of the BOD of waste waters. The current of the biofuel cell was decreased markedly with time until a steady state was reached. The steady state current was in all cases attained within 30–40 min at 37°C. A linear relationship was obtained between the steady state current and BOD. The BOD of industrial waste waters can be estimated by using the biofuel cell. Relative error of the BOD estimation was within ±10%. The current output of the biofuel cell was almost constant for 30 days. 相似文献
2.
Isao Karube Satoshi Mitsuda Shuichi Suzuki 《Applied microbiology and biotechnology》1979,7(4):343-350
Summary Whole cells of Pseudomonas fluorescens which utilized mainly glucose were immobilized in collagen membrane. The microbial electrode consisted of a bacteria-collagen membrane and an oxygen electrode was developed for the determination of glucose. When the electrode was inserted in a sample solution containing glucose, the current of the electrode decreased markedly with time until a steady state was reached. The response time of the electrode was 10 min by the steady state method. A linear relationship was observed between the steady state current and the concentration of glucose below 20 mg l
–1. The minimum concentration for determination was 2 mg of glucose per liter. The reproducibility of the current was examined using the same sample solution. The current was reproducible within ±6% of the relative error when a sample solution containing 10 mg {ie343-1} of glucose was employed. The standard deviation was 0.6 mg {ie343-2} in 20 experiments. The reusability of the glucose sensor was examined using the same sample solution (10 mg {ie343-3}). No decrease in current output was observed over a two week period and 150 assays. Glucose in molasses was determined with an average relative error of 10% by the microbial electrode sensor. 相似文献
3.
《Bioorganic & medicinal chemistry letters》1999,9(1):109-112
The ability of Clostridium butyricum cultures to hydrolyze three L-leucine-containing dipeptides (Leu-Leu, Leu-Gly and Gly-Leu) in a synthetic minimal medium is demonstrated by using gas chromatography coupled with mass spectrometry. The 13C nuclear magnetic resonance and a labeled dipeptide L-[1-13C]Leu-Gly were used to confirm this activity. 相似文献
4.
G. P. Gaenko E. V. Moiseeva O. Yu. Savel’ev Yu. G. Molotkovskii E. L. Vodovozova 《Microbiology》2009,78(5):580-584
Antitumor activity of the preparation of the lipid fraction of Clostridium butyricum spore extract was demonstrated in vivo on a transplantable mouse model of breast cancer. At a specific scheme of application,
inhibition of tumor growth and improved survival dynamics compared to the control group were observed. Thin-layer chromatography
(TLC) of the lipid fraction of the spore extract revealed, apart from a saturated hydrocarbon, cholesterol ester, cetyl palmitate,
triacyl glycerol, and palmitic acid, also a phenolic lipid bound in a complex with a peptide component. Acetone extraction
of the lipid pool revealed a phenolic lipid. According to the TLC and 1H-NMR spectrum of the acetone extract of the lipid fraction of C. butyricum spores, the structure of the phenolic lipid was proposed, n -butyl benzoate substituted in the para position. The phenolic lipid is suggested to be responsible for the biological activity of the spore’s lipid fraction. 相似文献
5.
【背景】丁酸梭菌是专性厌氧的新一代芽孢益生菌,耐热、耐酸、抗逆性强,极具应用价值和开发前景。【目的】优化丁酸梭菌发酵培养基并初步研究其发酵液对黄曲霉菌的抑制作用和降解黄曲霉毒素B1 (aflatoxin B1, AFB1)的能力。【方法】利用响应面法对发酵培养基进行优化,采用牛津杯法对丁酸梭菌发酵液抑制黄曲霉菌生长进行研究,并通过酶联免疫法测定发酵液对AFB1的降解能力。【结果】优化后的发酵培养基为:葡萄糖18.1g/L,大豆蛋白胨29.7g/L,磷酸氢二钾3.8 g/L,氯化钠2.0 g/L,乙酸钠4.0 g/L,结晶硫酸镁1.2 g/L,L-半胱氨酸盐酸盐0.3 g/L。优化后的丁酸梭菌生物量由8.99×108个/mL提高至2.28×109个/mL,是优化前的2.54倍。丁酸梭菌发酵液对致病真菌黄曲霉菌的抑菌效果十分显著,其上清液经浓缩后对AFB1降解72h的降解率达到68.65%,初步分析表明上清液中对AFB1 相似文献
6.
Effects of Acetate and Butyrate During Glycerol Fermentation by Clostridium butyricum 总被引:4,自引:0,他引:4
The effects of acetate and butyrate during glycerol fermentation to 1,3-propanediol at pH 7.0 by Clostridium butyricum CNCM 1211 were studied. At pH 7.0, the calculated quantities of undissociated acetic and butyric acids were insufficient
to inhibit bacterial growth. The initial addition of acetate or butyrate at concentrations of 2.5 to 15 gL−1 had distinct effects on the metabolism and growth of Clostridium butyricum. Acetate increased the biomass and butyrate production, reducing the lag time and 1,3-propanediol production. In contrast,
the addition of butyrate induced an increase in 1,3-propanediol production (yield: 0.75 mol/mol glycerol, versus 0.68 mol/mol
in the butyrate-free culture), and reduced the biomass and butyrate production. It was calculated that reduction of butyrate
production could provide sufficient NADH to increase 1,3-propanediol production. The effects of acetate and butyrate highlight
the metabolic flexibility of Cl. butyricum CNCM 1211 during glycerol fermentation.
Received: 2 January 2001 / Accepted: 6 February 2001 相似文献
7.
During batch fermentation of sucrose to butyric acid byClostridium butyricum the effect of growth factor supplementation was determined: addition of yeast extract (5 g/L) stimulated most. Using biotin
as the sole growth factor, average productivity was definitely lower. Beet molasses as a combined source of carbon and growth
factor were effective only at a high concentration (150 g/L). The optimal butyric acid production (45 g/L, yield 45%) was
achieved with sucrose concentration of 100 g/L in a medium supplemented with yeast extract (5 g/L). It represents an average
productivity of 0.90 gL−1 h−1 and relative butyric acid concentration of 91%. 相似文献
8.
Motohiko Hikuma Hiroshi Suzuki Yakeo Yasuda Isao Karube Shuichi Suzuki 《Applied microbiology and biotechnology》1979,8(4):289-297
Summary A microbial electrode consisting of immobilized living whole cells of yeasts, porous membrane and an oxygen electrode was prepared for continuous estimation of biochemical oxygen demand (BOD). Immobilized Trichosporon cutaneum was employed for the microbial electrode sensor for BOD. When a sample solution containing the equivalent amount of glucose and glutamic acid was injected into the sensor system, the current of the electrode decreased markedly with time until steady state was reached. The response time was within 18 min. A linear relationship was observed between the current decrease and the concentration below 41 mg l
– of glucose and 41 mg l
– glutamic acid (5-day BOD 60 mg l
–). The current decrease was reproducible within ± 6% of the relative error when a sample solution containing 27 mg l
– of glucose and 27 mg l
– of glutamic acid (5-day BOD 40 mg l
–) was employed. The microbial electrode sensor was applied to untreated waste waters from a fermentation factory. Good comparative results were obtained between BOD estimated by the microbial electrode and that determined by the conventional 5-day method (regression coefficient was 1.2). Furthermore, the effect of various compounds on BOD estimation was also examined. The current output of the microbial electrode sensor was almost constant for 17 d and 400 tests. 相似文献
9.
10.
Isao Karube Tadashi Okada Shuichi Suzuki Hiroshi Suzuki Motohiko Hikuma Takeo Yasuda 《Applied microbiology and biotechnology》1982,15(2):127-132
Summary A microbial sensor was prepared to determine sodium nitrite. This microbial sensor consisted of immobilized Nitrobacter sp. and an oxygen electrode. When a sample solution containing sodium nitrite was tested, nitrite was changed to NO2 gas in the buffer (pH 2.0) and the current of the electrode decreased with time until a steady state was reached. The steady state current was attained within 10 min and the maximum decrease in current was obtained at 30°C and pH 2.0. A linear relationship was observed between the current decrease and the sodium nitrite concentration below 0.59 mM, the minimum sodium nitrite concentration that could be determined was 0.01 mM. The current decrease was reproducible (5% relative error). The current output of the sensor was almost constant for more than 21 days and 400 assays. 相似文献
11.
Dr. E Petitdemange C Dürr S Abbad Andaloussi G Raval 《Journal of industrial microbiology & biotechnology》1995,15(6):498-502
Industrial glycerol obtained through the transesterification process using rapeseed oil did not support growth of several strains ofClostridium butyricum obtained from bacterial culture collections. Ten new strains ofC. butyricum were obtained from mud samples from a river, a stagnant pond, and a dry canal. These new isolates fermented the commercial glycerol and produced 1,3-propanediol as a major fermentation product with concomitant production of acetic and butyric acids. Four of the ten isolates were able to grow on industrial glycerol obtained from rapeseed oil. One strain,C. butyricum E5, was very resistant to high levels of glycerol and 1,3-propanediol. Using fed-batch fermentation, 109 g L–1 of industrial glycerol were converted into 58 g of 1,3-propanediol, 2.2 g of acetate and 6.1 g of butyrate per liter. 相似文献
12.
Chatzifragkou A Papanikolaou S Dietz D Doulgeraki AI Nychas GJ Zeng AP 《Applied microbiology and biotechnology》2011,91(1):101-112
The aim of the present study was to investigate the production of 1,3-propanediol (PDO) under non-sterile fermentation conditions
by employing the strain Clostridium butyricum VPI 1718. A series of batch cultures were performed by utilizing biodiesel-derived crude glycerol feedstocks of different
origins as the sole carbon source, in various initial concentrations. The strain presented similarities in terms of PDO production
when cultivated on crude glycerol of various origins, with final concentrations ranging between 11.1 and 11.5 g/L. Moreover,
PDO fermentation was successfully concluded regardless of the initial crude glycerol concentration imposed (from 20 to 80 g/L),
accompanied by sufficient PDO production yields (0.52–0.55 g per gram of glycerol consumed). During fed-batch operation under
non-sterile culture conditions, 67.9 g/L of PDO were finally produced, with a yield of 0.55 g/g. Additionally, the sustainability
of the bioprocess during a continuous operation was tested; indeed, the system was able to run at steady state for 16 days,
during which PDO effluent level was 13.9 g/L. Furthermore, possible existence of a microbial community inside the chemostat
was evaluated by operating a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis, and DGGE
results revealed the presence of only one band corresponding to that of C. butyricum VPI 1718. Finally, non-sterile continuous cultures were carried out at different dilution rates (D), with inlet glycerol concentration at 80 g/L. Maximum PDO production was achieved at low D values (0.02 h−1) corresponding to 30.1 g/L, while the elaboration of kinetic data from continuous cultures revealed the stability of the
bioprocess proposed, with global PDO production yield corresponding to 0.52 g/g. 相似文献
13.
González-Pajuelo M Andrade JC Vasconcelos I 《Journal of industrial microbiology & biotechnology》2004,31(9):442-446
Growth inhibition of Clostridium butyricum VPI 3266 by raw glycerol, obtained from the biodiesel production process, was evaluated. C. butyricum presents the same tolerance to raw and to commercial glycerol, when both are of similar grade, i.e. above 87% (w/v). A 39% increase of growth inhibition was observed in the presence of 100 g l–1 of a lower grade raw glycerol (65% w/v). Furthermore, 1,3-propanediol production from two raw glycerol types (65% w/v and 92% w/v), without any prior purification, was observed in batch and continuous cultures, on a synthetic medium. No significant differences were found in C. butyricum fermentation patterns on raw and commercial glycerol as the sole carbon source. In every case, 1,3-propanediol yield was around 0.60 mol/mol glycerol consumed. 相似文献
14.
Xin-liang Wang Wen-jie Chen Rui Jin Xuan Xu Jing Wei Hong Huang Yan-hua Tang Chang-wei Zou Ting-tao Chen 《Microbial biotechnology》2023,16(4):799-812
Hypertension is a significant risk factor of cardiovascular diseases (CVDs) with high prevalence worldwide, the current treatment has multiple adverse effects and requires continuous administration. The glucagon-like peptide-1 receptor (GLP-1R) agonists have shown great potential in treating diabetes mellitus, neurodegenerative diseases, obesity and hypertension. Butyric acid is a potential target in treating hypertension. Yet, the application of GLP-1 analogue and butyric acid in reducing blood pressure and reversing ventricular hypertrophy remains untapped. In this study, we combined the therapeutic capability of GLP-1 and butyric acid by transforming Clostridium butyricum (CB) with recombinant plasmid pMTL007 encoded with hGLP gene to construct the engineered probiotics Clostridium butyricum-pMTL007-GLP-1 (CB-GLP-1). We used spontaneous hypertensive rat (SHR) models to evaluate the positive effect of this strain in treating hypertension. The results revealed that the intragastric administration of CB-GLP-1 had markedly reduced blood pressure and improved cardiac marker ACE2, AT2R, AT1R, ANP, BNP, β-MHC, α-SMA and activating AMPK/mTOR/p70S6K/4EBP1 signalling pathway. The high-throughput sequencing further demonstrated that CB-GLP-1 treatments significantly improved the dysbiosis in the SHR rats via downregulating the relative abundance of Porphyromonadaceae at the family level and upregulating Lactobacillus at the genus level. Hence, we concluded that the CB-GLP-1 greatly improves blood pressure and cardiomegaly by restoring the gut microbiome and reducing ventricular hypertrophy in rat models. This is the first time using engineered CB in treating hypertension, which provides a new idea for the clinical treatment of hypertension. 相似文献
15.
Hiromichi Kayano Tadashi Matsunga Isao Karube Shuichi Suzuki 《Biotechnology and bioengineering》1981,23(10):2283-2291
Immobilized chloroplasts and Clostridium butyricum were employed for a photochemical energy conversion system. Spinach chloroplasts were immobilized in 2% agar gel. The optimum temperature of immobilized chloroplasts was 30°C. The maximum activity was obtained in the phosphate buffer solution (pH 8.0) containing 8μM of ferredoxin under an N2 bubbling condition. Hydrogen was evolved under illumination by immobilized chloroplasts and C. butyricum. Hydrogen produced by this system was applied to a hydrogen-oxygen fuel cell. Photoinduced current was obtained from this photochemical energy conversion system. A photocurrent of 0.4?1.5 mA was continuously obtained for 4 h. The conversion ratio from hydrogen to current was 80?100%. 相似文献
16.
Aims: To characterize the antimicrobial and adhesion ability of candidate probiotic Clostridium butyricum CB2 for farmed fish in vitro. Methods and Results: The potential probiotic Cl. butyricum CB2 had been evaluated for its adhesion capacity and antagonistic effect against two fish pathogens Aeromonas hydrophila and Vibrio anguillarum by the intestinal cell model. In addition, the aggregation ability and antimicrobial property on agar plate were assayed. The results indicated that the candidate probiotic Cl. butyricum CB2 have strong adhesion property and a higher antagonistic activity to Aer. hydrophila and V. anguillarum both on agar plate and cell model. Clostridium butyricum showed a higher aggregation which might be the reasons for bacteria adhesion and antimicrobial activity. Conclusions: The strain Cl. butyricum CB2 could be used as potential probiotic to inhibit pathogens growth and prevent their colonization in fish intestinal tract. Significance and Impact of the Study: This study revealed the antimicrobial and adhesion characteristic of Cl. butyricum CB2 which was selected as the potential probiotic to farmed fish. 相似文献
17.
Various pectinolytic strains ofClostridium, Erwinia, andPseudomonas species produced methanol as a major end product during growth on pectin but not on glucose or polygalacturonic acid. Pectin
metabolism ofClostridium butyricum strain 4P1 correlated with a final product concentration of 16 mM at the end of growth, and a 1:1 stoichiometry for methanol
production and percent initial substrate methoxylation. Growth on pectin was associated with high activity of pectin methylesterase
and the absence of methanol consumption. The ecological significance of pectin metabolism and the establishment of microbial
methylotrophic metabolism in nature is discussed. 相似文献
18.
G. R. Zoutberg R. Willemsberg G. Smit M. J. Teixeira de Mattos O. M. Neijssel 《Applied microbiology and biotechnology》1989,32(1):22-26
Summary When an aggregate-forming variant of Clostridium butyricum was grown in continuous culture under glucose-limited conditions (pH 5.2, dilution rate = 0.1 h–1, 30° C), glucose was mainly fermented to isopropanol and butanol. Production of solvents was pH dependent and initiated above a threshold concentration of acetic and butyric acid, which probably means that it was triggered by the undissociated concentration of these acids. This was confirmed by studies with a second reactor. Solvent productivity by this variant was found to be stable and the highest ever reported for Clostridium sp. in argued that, due to the aggregated growth mode, this variant is able to reach the threshold concentration of acids at which solventogenesis is initiated at high dilution rates.Offprint requests to: G. R. Zoutberg 相似文献
19.
Nobuhiro Fujii Kouichi Kimura Noriko Yokosawa Keiji Oguma Teruo Yashiki Kouichi Takeshi Touru Ohyama Emiko Isogai Hiroshi Isogai 《Microbiology and immunology》1993,37(5):395-398
The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb HindIII fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C.butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component. 相似文献
20.
M. Heyndrickx P. De Vos A. Speybrouck J. De Ley 《Applied microbiology and biotechnology》1989,31(4):323-328
Summary The continuous fermentation of mannitol (pH 6, dilution rate (D)=0.087 h-1) by Clostridium butyricum LMG 1213t1 was investigated under several conditions. Mannitol was readily fermented when glucose or acetate were added in the in-flow medium as co-substrate. Butyrate, CO2 and H2 were the major fermentation products. In mannitol-glucose mixtures (ratios 4 or 8) the amount of mannitol fermented depended upon the amount of glucose in the in-flow medium. In mannitol-acetate mixtures, 1 mol of acetate was needed for the fermentation of approximately 5.5 mol mannitol. We detected d-mannitol-1-phosphate dehydrogenase activity, responsible for the generation of supplementary reduced nicotine adenine dinucleotide (NADH) as a source for extra H2 gas. Fermentation of mannitol-acetate in the presence of [14C]-labelled acetate revealed butyrate as the only labelled fermentation end-product. 相似文献