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Morphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts. 下载免费PDF全文
Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary forms. With time-lapse photomicrography, reversion sequences of single protoplasts were demonstrated. 相似文献
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Effect of glutaraldehyde on protoplasts of Bacillus megaterium 总被引:5,自引:0,他引:5
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In the present study the possibility of obtaining interspecific bacterial hybrids by polyethylene glycol-assisted fusion of protoplasts from Bacillus thuringiensis and Bac. megaterium has been examined. Electron microscopic and genetic data allow to confirm with great probability that cytological fusion takes place. However, genetic analysis revealed that neither of methods applied for protoplast fusion gave stable recombinants. Apparently, it is due to the lack of recombination or the death of recombinants that follows the functioning of the cell correction system. The mechanism of protoplast fusion and its most important steps are also studied in the present work. 相似文献
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Polyethylene glycol-induced fusion of heat-inactivated and living protoplasts of Bacillus megaterium. 总被引:4,自引:4,他引:0 下载免费PDF全文
Protoplasts of Bacillus megaterium, incubated at 50 degrees C for 120 min, lost the ability to revert to bacillary form. Such heat-inactivated protoplasts, however, produced recombinants when fused by polyethylene glycol treatment with normal protoplasts. Although this differential inactivation effect is not yet fully reproducible, reciprocal inactivations of the parental protoplasts in genetic crosses have clearly shown that for protoplast fusion (i) either of the parents may serve as the viable recipient for markers coming from the heated parental protoplasts, and (ii) either of the parents may be rendered nonviable and yet, when fused with a viable partner, contribute to formation of a recombinant. Heat inactivation seems to provide a way to counterselect when few markers are available and one of the parents is prototrophic. 相似文献
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Lajos Alfldi 《BioEssays : news and reviews in molecular, cellular and developmental biology》1996,18(1):77-81
This essay describes the author's studies with bacteria in post-war Hungary; the difficulties encountered, with funding, collaboration and publication; and how the Szeged Institute consolidated itseif as the one outstanding scientific Institute in Eastern Europe, with the author at the helm. 相似文献
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Electron microscopic observations on growing and dividing protoplasts of Bacillus megaterium 总被引:1,自引:0,他引:1
I Kusaka 《Journal of general microbiology》1970,63(2):199-202
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Studies on the morphology and nucleic acid content of protoplasts of Bacillus megaterium 总被引:2,自引:2,他引:0 下载免费PDF全文
FITZ-JAMES PC 《Journal of bacteriology》1958,75(4):369-382
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The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme 总被引:68,自引:38,他引:30 下载免费PDF全文
WEIBULL C 《Journal of bacteriology》1953,66(6):688-695
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