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1.
Protein G genes: structure and distribution of IgG-binding and albumin-binding domains 总被引:6,自引:0,他引:6
Protein G (also designated Fc receptor type III) is the IgG-binding protein of group C and G streptococci. Protein G has also been shown to bind human serum albumin but at a site that is structurally separated from the IgG-binding region. From the known gene sequence of protein G, two synthetic oligonucleotides were constructed for use as probes in DNA-hybridization experiments to study the structure and distribution of the albumin- and IgG-binding regions in bacterial strains belonging to different species. Thus, one of the probes corresponded to repeats within the IgG-binding region (I) and the other corresponded to repeats in the albumin-binding encoding region (II). Probe I showed strong hybridization to DNA isolated from 31 human group C and G strains, whereas hybridization to probe II was variable. With the three restriction endonucleases used, three restriction patterns were found in Southern blot experiments. No fundamental difference could be detected in hybridization experiments, either between strains of group C and G streptococci, or between isolates of different clinical origin. No hybridization to DNA from other bacterial species was found. 相似文献
2.
Nitsche-Schmitz DP Johansson HM Sastalla I Reissmann S Frick IM Chhatwal GS 《The Journal of biological chemistry》2007,282(24):17530-17536
Recent epidemiological data on diseases caused by beta-hemolytic streptococci belonging to Lancefield group C and G (GCS, GGS) underline that they are an emerging threat to human health. Among various virulence factors expressed by GCS and GGS isolates from human infections, M and M-like proteins are considered important because of their anti-phagocytic activity. In addition, protein G has been implicated in the accumulation of IgG on the bacterial surface through non-immune binding. The function of this interaction, however, is still unknown. Using isogenic mutants lacking protein G or the M-like protein FOG (group G streptococci), respectively, we could show that FOG contributes substantially to IgG binding. A detailed characterization of the interaction between IgG and FOG revealed its ability to bind the Fc region of human IgG and its binding to the subclasses IgG1, IgG2, and IgG4. FOG was also found to bind IgG of several animal species. Surface plasmon resonance measurements indicate a high affinity to human IgG with a dissociation constant of 2.4 pm. The binding site was localized in a central motif of FOG. It has long been speculated about anti-opsonic functions of streptococcal Fc-binding proteins. The presented data for the first time provide evidence and, furthermore, indicate functional differences between protein G and FOG. By obstructing the interaction between IgG and C1q, protein G prevented recognition by the classical pathway of the complement system. In contrast, IgG that was bound to FOG remained capable of binding C1q, an effect that may have important consequences in the pathogenesis of GGS infections. 相似文献
3.
Comparative characteristics of protein G gene in Streptococci of groups C and G isolated from humans
Volchek NA Gupalova TV Totolian AA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(5):7-11
The treatment of streptococci, groups C and G, with bromocyanogen made it possible to isolate surface G protein, capable of binding human serum albumin (HSA) and polyclonal IgG. In this work the presence of G protein in all staphylococcal strains, groups C and G, is shown. The differences between the strains by the level of expression, molecular weight and functional activity of G protein, extracted from streptococci of groups C and G, permitted the identification of 3 groups of strains, containing the molecules of G protein with different numbers of IgG- and HSA-binding domains: with 3 IgG- and HSA-binding domains, with 2 IgG- and HSA-binding domains and with only 2 IgG-binding domains. Each strain under study expressed only one of the molecule of G protein. The work shows the possibility of the identification of streptococci, groups C and G, by the molecular characteristics of G proteins themselves and their respective coding genes. 相似文献
4.
The IgG-Fc binding activity and binding sites on the cell surface of streptococci, strains AR1 (group A) and G148 (group G), and Staphylococcus aureus strain Cowan I were examined by hemagglutination (HA) and immunoelectron microscopic methods. No distinct difference was observed in the HA activity among these three strains. However, the strains differed in the distribution of Fc receptor. Cowan I cells (having protein A) were heavily covered with two layers of ferritin particles, whereas AR1 cells were heavily covered with a single, rough layer of ferritin particles. G148 cells (having protein G) were labeled with a relatively thin, rough ferritin layer. The trypsin susceptibility of the Fc receptors of the AR1 strain was much higher than that of the G148 strain. These results suggest that both streptococcal strains are distinctly different in the arrangement or in the conformation of the Fc receptor from the Cowan I strain. It is also suggested that the Fc receptor molecules of the streptococcal strains differ from each other not only in conformation but also in trypsin susceptibility. 相似文献
5.
The SmaI restriction endonuclease digestion
patterns of chromosomal DNAs from 99 pyogenic streptococci belonging to
Lancefield group A (41 Streptococcus pyogenes), group C (seven S.
dysgalactiae, 11 \QS. equisimilis\W, three S. equi, eight
S. zooepidemicus) and group G (25 human group G
Streptococcus, four S. canis) were analyzed by pulsed-field
gel electrophoresis (PFGE), and the results were compared with those
previously obtained by multilocus enzyme electrophoresis (MLEE) and random
amplified polymorphic DNA analysis (RAPD). PFGE revealed 93 distinct types
among the 99 strains, and no patterns were common to strains of different
species. The discriminatory power of PFGE was greater than that of MLEE and
RAPD for groups A and G streptococci. The polymorphism among group C
streptococci was similar with the three techniques. PFGE is, therefore, the
most efficacious method for epidemiological typing of pyogenic streptococci.
Received: 31 July 1996 / Accepted: 17 August 1996 相似文献
6.
A monoclonal antibody giving a dominant reaction with the group-specific polysaccharide of streptococcus group B in an ELISA
test has been developed. The purified polysaccharide exhibited a high positivity with reference anti B streptococcal antiserum
in the ELISA test. Cross-tests of antibodies with other groups of streptococci provided a minimum cross-reaction only in the
case of G streptococci. Monoclonal antibodies were prepared usingStreptococcus agalactiœ S 589 MT strain isolated from a case of bovine mastitis which does not express Ia, Ib, II, III, IV and V type antigens, nor
C, R and X protein antigens. 相似文献
7.
Claude Sauvage Jean-François Rumigny Michel Maitre 《Molecular and cellular biochemistry》1991,107(1):65-77
Summary Three G proteins from human brain membranes were purified to near homogeneity by conventional techniques including preparative
electrophoresis. These G proteins were characterized by their ability to bind GTP, GDP and GTP analogs. Two of these proteins
have molecular weights of 50,000 (G50) and 36,000 (G36), as determined on SDS-gels. G36 was ADP-ribosylated by pertussis toxin. Thus, G50 could represent a Gsα subunit, whereas G36 could be Giα or Goα. G50 was phosphorylated by cAMP dependent protein kinase and protein kinase C. G36 was phosphorylated by a protein kinase independent of calcium and phospholipid, a proteolytic product of protein kinase C,
analogous to protein kinase M. Phosphorylation of G36 by this protein kinase induced a dramatic decrease in its GTPase activity. The third G protein, of molecular weight 22,000
probably belongs to the group of monomeric G proteins possessing functional similarities withras gene products. The regulation of G proteins involving calcium-dependent and independent pathways is delineated. 相似文献
8.
Protein G was solubilized from 31 human group C and G streptococcal strains with the muralytic enzyme mutanolysin. As judged by the mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the binding patterns of the solubilized protein G molecules in Western blot experiments, the strains could be divided into three groups, represented by the group G streptococcal strains G148 and G43 and the group C streptococcal strain C40. The 65-kDa G148 protein G and the 58-kDa C40 protein G showed affinity for both immunoglobulin G (IgG) and human serum albumin (HSA), whereas the 40-kDa G43 protein G bound only IgG. Despite the different molecular patterns, the three protein G species had identical NH2-terminal amino acid sequences. Apart from the 65-kDa peptide, digestion of G148 streptococci with mutanolysin also produced a 52-kDa IgG- and HSA-binding peptide and a 14-kDa HSA-binding peptide. It was demonstrated that these peptides resulted from cleavage of 65-kDa protein G by proteolytic components in the mutanolysin preparation. The protein G genes of the C40 and G43 strains were cloned and sequenced, and their structure was compared to the previously published sequence of the G148 protein G gene. As compared to G148, both the C40 and G43 genes lacked a 210-base pair fragment in the IgG-binding region, accounting for the 10-fold lower affinity of these proteins for IgG. The G43 gene also lacked a 450-base pair fragment in the 5'-end of the gene, explaining why the G43 protein G did not bind HSA. The differences in protein G structure did not correlate with the clinical origin of the strains used in this study. The IgG-binding region of protein G was further mapped. Thus, a peptide corresponding to a single IgG-binding unit was obtained by the cloning and expression of a 303-base pair polymerase chain reaction-generated DNA fragment. The affinity of this 11.5-kDa peptide for human IgG was 8.0 x 10(7) M-1, as determined by Scatchard plots. Finally, a 55-amino acid-long synthetic peptide, corresponding to one of the three repeated domains in the COOH-terminal half of strain G148 protein G, effectively blocked binding of protein G to IgG. 相似文献
9.
Bavi RS Kamble AD Kumbhar NM Kumbhar BV Sonawane KD 《Cell biochemistry and biophysics》2011,61(3):507-521
Conformational preferences of the modified nucleosides N2-methylguanosine (m2G) and N2, N2-dimethylguanosine (m22G) have been studied theoretically by using quantum chemical perturbative configuration interaction with localized orbitals
(PCILO) method. Automated complete geometry optimization using semiempirical quantum chemical RM1, along with ab initio molecular
orbital Hartree–Fock (HF-SCF), and density functional theory (DFT) calculations has also been made to compare the salient
features. Single-point energy calculation studies have been made on various models of m2G26:C/A/U44 and m22G26:C/A/U44. The glycosyl torsion angle prefers “syn” (χ = 286°) conformation for m2G and m22G molecules. These conformations are stabilized by N(3)–HC2′ and N(3)–HC3′ by replacing weak interaction between O5′–HC(8).
The N2-methyl substituent of (m2G26) prefers “proximal” or s-trans conformation. It may also prefer “distal” or s-cis conformation that allows base pairing with A/U44 instead of C at the hinge region. Thus, N2-methyl group of m2G may have energetically two stable s-trans m2G:C/A/U or s-cis m2G:A/U rotamers. This could be because of free rotations around C–N bond. Similarly, N2, N2-dimethyl substituent of (m22G) prefers “distal” conformation that may allow base pairing with A/U instead of C at 44th position. Such orientations of
m2G and m22G could play an important role in base-stacking interactions at the hinge region of tRNA during protein biosynthesis process. 相似文献
10.
In an attempt to assess the taxonomic relationships among human (Corynebacterium haemolyticum), animal (Corynebacterium pyogenes bovis) haemolytio corynebacteria, typical corynebacteria (Corynebacterium diphteriae mitis, C. ovis, C. ulcerans) and group A and G streptococci, a number of biochemical parameters were established: the DNA content of G + C, the presence
of the cytochrome system, composition of fatty acids in free lipids and production of carboxylic acids as end products of
fermentation. It was found that according to the above criteria, streptococci differed significantly from the corynebacteria
studied. In addition, it was possible to differentiate a subgroup of typically aerobic haemolytic corynebacteria (different
from both human and animal corynebacteria), possessing a complete cytochrome system, producing propionic acid and having a
different composition of fatty acids. 相似文献
11.
Protein G, the immunoglobin G-binding surface protein of group C and G streptococci, also binds serum albumin. The albumin-binding site on protein G is distinct from the immunoglobulin G-binding site. By mild acid hydrolysis of the papain-liberated protein G fragment (35 kDa), a 28-kDa fragment was produced which retained full immunoglobulin G-binding activity (determined by Scatchard plotting) but had lost all albumin-binding capacity. A protein G (65 kDa), isolated after cloning and expression of the protein G gene in Escherichia coli, had comparable affinity to immunoglobulin G (5-10 X 10(10)M-1), but much higher affinity to albumin than the 35- and 28-kDa protein G fragments (31, 2.6, and 0 X 10(9)M-1, respectively). The amino-terminal amino acid sequences of the 65-, 35-, and 28-kDa fragments allowed us to exactly locate the three fragments in an overall sequence map of protein G, based on the partial gene sequences published by Guss et al. (Guss, B., Eliasson, M., Olsson, A., Uhlen, M., Frej, A.-K., J?rnvall, H., Flock, J.-I., and Lindberg, M. (1986) EMBO J. 5, 1567-1575) and Fahnestock et al. (Fahnestock, S. R., Alexander, P., Nagle, J., and Filpula, D. (1986) J. Bacteriol. 167, 870-880). In this map could then be deduced the location of three homologous albumin-binding regions and three homologous immunoglobulin G-binding regions. 相似文献
12.
Bacteriophages from streptococci of groups A, E, and G lysed streptococci distributed among serogroups A, C, G, H, and L; propagated in some, but not all, of these; and transduced streptomycin resistance within group A or (by A phages only) from group A to group G streptococci. 相似文献
13.
Polymorphic markers of some genes associated with multiple sclerosis in the population of Kazakhstan
N. A. Aitkhozhina V. G. Nigmatova A. K. Khanseitova M. A. Mendesh E. E. Ashirbekov T. S. Balmukhanov 《Russian Journal of Genetics》2011,47(6):749-753
Associations of DR2 specificity of the DRB1 gene and single-nucleotide polymorphisms of the tumor necrosis factor gene TNFα (−308 G/A), interleukin genes IL-1β (−511 C/T), IL-2 (−475 A/T and −631 G/A), IL-6 (−634 C/G), paraoxanase gene PON1 (M55L, Q192R), and the mitochondrial protein transport gene UCP2 (−866 G/A) with the development of multiple sclerosis (MS) were studied in two main ethnic groups of Kazakhstan (Kazakhs
and Russians). An association of DR2 specificity of the DRB1 gene with MS was found in the combined group of Kazakhs, Russians, and offsprings from mixed marriages. No correlation between
DR2 specificity and MS was found in the separately examined groups of Kazakhs and Russians. Statistically significant (p < 0.05) differences between the MS patients and healthy individuals were observed in the distribution of the genotypes at
site −634 G/C of the IL-6 gene in the Kazakh group, in the allelic frequencies at site −308 A/G in the promoter region of the TNFα gene in the Russian group, and in the frequencies of alleles at the polymorphic Q192R locus of the PON1 gene in the Kazakh group.. No significant differences were revealed in the distribution of the genotypes and in the frequencies
of alleles at the polymorphic sites of the genes IL-1β (−511 C/T), IL-2 (−475 A/T and −631 G/A), PON1 (M55L), and UCP2 (−866 G/A). 相似文献
14.
Fibrinogen binding inhibits the fixation of the third component of human complement on surface of groups A, B, C, and G streptococci 总被引:9,自引:0,他引:9
Effects of fibrinogen binding to M protein-positive and -negative streptococci on fixation of the third component of human complement (C3) were determined. In all test cultures of serological groups A, B, C, and G fixation of C3 was observed in normal human serum as revealed by quantitative fluorescent immunoassay. Fibrinogen binding inhibited the fixation of C3 on streptococci. The degree of inhibition was proportional to the extent of fibrinogen binding. Thus, inhibition of C3 fixation was most pronounced in strongly fibrinogen-positive streptococci of groups A, C, and G and not demonstrable in fibrinogen-negative cultures of groups C and G. Trypsinization of the streptococci destroyed their capacity to bind fibrinogen and consequently the inhibitory effects on C3 fixation. The carboxymethylated alpha and beta chains of fibrinogen moderately inhibited C3 fixation whereas gamma chain had no influence. These studies may indicate that fibrinogen binding structures other than M protein could also be involved in streptococcal pathogenicity. 相似文献
15.
This study aimed to detect differences in the richness of total supragingival plaque microbiota as well as the species composition
of oral streptococci involved in the different stages of dental caries. Forty-five plaque samples were collected from caries-moderate
(CM, 4 ≤ dmfs ≤ 6), caries-susceptible (CS, dmfs ≥ 10), and age-matched caries-free children separately. Total DNA was isolated
directly from each sample, and polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) analyses using
universal and primers specific for oral streptococci were carried out. Using 16S rDNA PCR-DGGE, 34 different species of bacteria
were identified in a culture-independent manner and classified into 11 genera according to phylogenetic analysis. Among them,
Mitis group streptococci and Campylobacter, which were present in health status, no longer appeared in caries-susceptible samples. In addition, Capnocytophaga, Burkholderia, and Prevotella were found significantly less frequently in the CS group samples (P < 0.05), while there were no significant differences among the prevalence of Neisseria, Leptotrichia, Haemophilus, Mutans group streptococci, Corynebacterium, and Actinomyces in the three groups. Further DGGE analysis of rnpB gene amplicons obtained with oral streptococci species-specific primers showed that a total of 23 species of oral streptococci
were identified. Streptococcus sanguinis, Streptococcus mitis, and Streptococcus oralis showed a significantly higher prevalence in healthy children (P < 0.05), while that of Streptococcus mutans and Streptococcus sobrinus did not vary among the three groups. Overall, these results suggest that supragingival plaque microbiota as a whole undergoes
a more complicated shift in the caries process. 相似文献
16.
PCR generated fragments of the protein G gene from three GCS and GGS strains belonging to different G types had been cloned.
The resulting PCR products were cloned intoE. coli using expression vector pQE31. The clones, producing IgG-binding peptides were selected. Recombinant plasmids carried different
inserts and encoded proteins of different size and with different binding properties.
Presented at theInternational Conference on Recent Problems in Microbiology and Immunology, Košice (Slovakia), 13–15 October 1999. 相似文献
17.
L. Z. Akhmadishina G. F. Korytina T. V. Victorova 《Russian Journal of Genetics》2011,47(10):1248-1255
The contribution of the polymorphic markers of cytochrome P450 genes to respiratory diseases caused by smoking and occupational
factors has been assessed. For this purpose, PCR-RFLP analysis of the CYP1B1 (rs1056836, 4326C > G), CYP2F1 (rs11399890, c.14_15insC), CYP2J2 (rs890293, -76G > T), and CYP2S1 (rs34971233, 13106C > T and rs338583, 13255A > G) gene polymorphisms has been performed. The analysis has shown that CYP1B1 (rs1056836, 4326C > G) and CYP2F1 (rs11399890, c.14_15insC) polymorphisms may contribute to the development of occupational chronic bronchitis. The proportion
of CYP1B1*1*3 heterozygotes in the group of patients with occupational chronic bronchitis is considerably greater than in the group of
healthy workers (69.16% versus 53.29%; χ2 = 5.94, p = 0.02, p
cur = 0.04, OR = 1.97, the 95% CI is 1.13–3.42). Patients with occupational chronic bronchitis and healthy workers significantly
differed from each other in the CYP2F1 genotypes frequency distribution (rs11399890, c.14_15insC) (χ2 = 6.18, d.f. = 2, p = 0.05). CYP2F1 wild type/ins heterozygous genotype frequency is higher in healthy workers (36.08%) than in patients (22.22%) (χ2 = 5.48, p = 0.02, p
cur = 0.04, OR = 0.51, the 95% CI is 0.28–0.90). No association has been found between the CYP2J2 (rs890293, −76G > T) or CYP2S1 (rs34971233, 13106C > T, and rs338583, 13255A > G) gene polymorphisms and respiratory diseases. 相似文献
18.
Specific binding sites for monomeric and aggregated beta 2-microglobulin on surface of groups A, B, C, and G streptococci 总被引:2,自引:0,他引:2
Human beta 2-microglobulin (beta 2-m) was isolated from urine samples of patients with tubular dysfunctions and aggregated with glutaraldehyde. Four aggregates with molecular weights of 800,000, 480,000, 260,000, and 60,000 were separated by filtration on Sephacryl S-300. The aggregates and monomeric beta 2-m (11,800 MW) were subsequently labeled with 125I and tested for binding to streptococci. Group A streptococci bound only aggregated beta 2-m with a mean binding of 44.5%. Most of the group G streptococci, on the other hand, bound only monomeric beta 2-m with a mean binding of 58%. Among group B streptococci the serotypes with protein antigens interacted mainly with monomeric beta 2-m and those without protein antigens preferentially with aggregated beta 2-m. Nontypable group B streptococcal serotypes did not bind monomeric or aggregated beta 2-m. Of the streptococci belonging to group C, S. equisimilis reacted with monomeric beta 2-m and S. dysgalactiae with aggregated beta 2-m. S. equi did not interact with monomeric beta 2-m or aggregated beta 2-m. Bindings of monomeric beta 2-m and aggregated beta 2-m were saturable and could be inhibited by the respective unlabeled forms of beta 2-m. Fibrinogen, fibronectin, alpha 2-macroglobulin, haptoglobin, or immunoglobulin G did not inhibit the binding of either form of beta 2-m. The binding sites for monomeric beta 2-m were more susceptible to trypsin than those for aggregated beta 2-m. Treatment of streptococci with pronase destroyed their binding activities for monomeric and aggregated beta 2-m. Both monomeric beta 2-m and aggregated beta 2-m binding sites were sensitive to heat. The Scatchard plots of monomeric beta 2-m and aggregated beta 2-m were linear with Kd of 1.29 X 10(-9) M and 1.9 X 10(-9) M respectively. The number of binding sites per bacterium were estimated to be 81,000 for monomeric beta 2-m and 1,210 for aggregated beta 2-m. 相似文献
19.
Lysis and Lysogenization of Groups A, C, and G Streptococci by a Transducing Bacteriophage Induced from a Group G Streptococcus 总被引:5,自引:2,他引:3
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A temperate bacteriophage, designated GT-234, was isolated from a group G Streptococcus after ultraviolet irradiation. After several single-plaque passages in a group G indicator strain, this phage formed plaques in 3 of 14 group A strains, in 3 of 15 group C strains, and in 4 of 13 group G strains-but not in some representatives of several other serogroups. After propagation in each of the sensitive strains, the progeny from each was shown to be the same phage by (i) adsorption and plaque formation in each of the other groups, (ii) lysogenization in each of the other groups, (iii) high titers on infection of each serogroup, regardless of the group of propagating strain, and (iv) neutralization of infection in each of the other groups by antiserum against the phage propagated in group G. Phage GT-234 is serologically related to virulent group A phage A25, from which it is morphologically indistinguishable. Like A25, it is a transducing phage. Other studies showed that A25, as well as a group A temperate transducing phage (AT-298), could also infect strains of group C and G. These results indicate a need for reassessment of group specificity and phage receptors among streptococci of groups A, C, and G and raise possibilities for intergroup transduction. 相似文献
20.
B. F. Pan X. Dai Z. Y. Li O. P. Ward 《World journal of microbiology & biotechnology》1997,13(1):17-20
The conditions for the reduction of dibenzoyl by silicone-immobilizedGeotrichum sp. G38 were examined, and optimal concentrations of stannous octanoate, ethyl silicate, water and entrapped biomass for
the reaction were established. The optimum pH and temperature of the reaction medium were 7.0, and 35‡C (free cells) or 40‡C
(immobilized cells), respectively. The immobilized cells showed higher activity than free cells under optimum conditions.
The extent of conversion remained greater than 90% even after immobilized cells had been recycled 28 times. When silicone-immobilizedGeotrichum sp. G38 was used in the reduction of ethyl benzoylacetate, the (R)-enantiomer was obtained in an 81% enantiomeric excess
compared with 49% enantiomeric excess using free cells. 相似文献