共查询到20条相似文献,搜索用时 31 毫秒
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HOUCK JC 《Journal of applied physiology》1958,13(2):273-277
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ANFINSEN CB 《Federation proceedings》1957,16(3):783-791
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In addition to RNase H(70) two other proteins of Saccharomyces cerevisiae exhibit ribonuclease H activity 总被引:2,自引:0,他引:2
Two ribonucleases H (RNases H) were purified to apparent homogeneity from the yeast Saccharomyces cerevisiae. The enzymes were separated from the previously described yeast ribonuclease H (RNase H(70), Karwan, R., Blutsch, H., and Wintersberger, U. (1983) Biochemistry 22, 5500-5507) by chromatography on Mono Q and blue-Sepharose columns and from each other on a Mono S column. The two proteins, RNase H(55) of molecular weight around 55,000 and RNase H(42) of molecular weight around 42,000, exhibit distinct enzymatic properties: RNase H(55) acts as a 5'-exonuclease of low specific activity and produces predominantly monoribonucleotides from the synthetic hybrid poly(rA)-poly(dT). RNase H(42) efficiently releases oligoribonucleotides from the same substrate. Polyclonal antibodies against these proteins do not cross-react with RNase H(70), and thus, these two RNases H probably do not represent proteolytic breakdown products of RNase H(70). Peptide maps obtained by total digestion of RNase H(55) and RNase H(42) with trypsin reveal several common peptides and, therefore, suggest that the two enzymes are related to each other. We tentatively conclude that RNase H(55) is proteolytically processed to RNase H(42) in vivo. 相似文献
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Rodríguez M Moussaoui M Benito A Cuchillo CM Nogués MV Vilanova M 《Archives of biochemistry and biophysics》2008,471(2):191-197
Analyzing the pattern of oligonucleotide formation induced by HP-RNase cleavage shows that the enzyme does not act randomly and follows a more endonucleolytic pattern when compared to RNase A. The enzyme prefers the binding and cleavage of longer substrate molecules, especially when the phosphodiester bond that is broken is 8-11 nucleotides away from at least one of the ends of the substrate molecule. This more endonucleolytic pattern is more appropriate for an enzyme with a regulatory role. Deleting two positive charges on the N-terminus (Arg4 and Lys6) modifies this pattern of external/internal phosphodiester bond cleavage preference, and produces a more exonucleolytic enzyme. These residues may reinforce the strength of a non-catalytic secondary phosphate binding (p2) or, alternatively, constitute a new non-catalytic phosphate binding subsite (p3). 相似文献
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The activities of RNA polymerase I and Mg2+ - dependent ribonuclease H (RNase H) become doubled in the liver nuclei of rats that are fed for several days a diet that lacks amino acids or protein. Both Mn2+- and Mg2+- dependent RNases H activities increased two- to three-fold in the liver nuclei of rats in which hepatic DNA replication has been induced by a dietal manipulation. 相似文献
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Nuclear location of ribonuclease H and increased level of magnesium-dependent ribonuclease H in rat liver on thioacetamide treatment 总被引:1,自引:0,他引:1
Rat liver nuclei were isolated in aqueous solutions of low ionic strength or anhydrous glycerol. The presence of ribonuclease H (RNase H) [EC 3.1.4.34] activity in the cytoplasm is due to extraction of the nuclear enzyme by buffer and inorganic salts. Two forms of RNase H were separated from rat liver nuclei by affinity chromatography using a DNA-cellulose column. When the RNase H in the wash solution of nuclei with 0.3 M sucrose and in nuclear solution extracted with 0.15 M NaCl were fractionated on a single-stranded DNA-cellulose column, two peaks corresponding to Mn2+- and Mg2+-dependent RNases H were eluted at 0.1 M and 0.2 M NaCl, respectively, and a peak having both RNase H activities was recovered in the wash-through fraction from the column. Among the enzymes from these two fractions in the nuclei, the activity of the Mg2+-dependent RNase H which binds to DNA-cellulose increased several-fold within 24 h of a single injection of thioacetamide. The activities of Mg2+-dependent RNase H extracted with higher-salt solution from the nuclei and recovered in the flow-through fraction from the DNA-cellulose column and the Mn2+-dependent RNase H activities were relatively unaffected by an injection of thioacetamide. 相似文献
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Magdalena Rucka 《Biotechnology Techniques》1987,1(3):189-194
Summary RNase was immobilized by entrapment in polymer matrix during the formation of the ultrafiltration membrane. The resulting membrane can hydrolyze RNA with simultaneous separation of reaction products (nucleotides) and RNA. The RNase activity has not decreased after one month of operation. 相似文献
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Estrogen-induced ribonuclease activity in Xenopus liver 总被引:6,自引:0,他引:6
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