Role of micromorphological leaf traits and molecular data in taxonomy of three sympatric white oak species and their hybrids (Quercus L.)

Hybridisation and introgression occur with high frequency in the genus Quercus and interspecific hybrid individuals show patterns of morphological traits that might be influenced in different ways. Micromorphological leaf traits appear to be positive and stable in Quercus species, and by combining genetic and micromorphological analyses, it is possible to compare the patterns of variation in micromorphological leaf traits of pure and hybrid individuals. Trichomes and stomatal traits were examined using scanning electron microscopy at 150–2000 × magnification in sympatric oak species collected in a natural deciduous wood. Q. frainetto, Q. petraea and Q. pubescens appear to have a relatively predictable complement of trichome types. Both the pattern and quantitative values of each micromorphological trait examined (stomata and trichomes) have an important role in identifying hybrids and pure species; putative hybrids show a pattern of trichomes that is a combination of the parental types. These results, combined with the fact that micromorphological traits generally exhibit higher consistency, indicate that this source of information can be an excellent clue to hybridisation and introgression and useful in taxonomical, systematic and evolutionary studies on the European white oaks.     

Germination ecology of three endangered river corridor plants in relation to their preferred occurrence

As a contribution to conservation, we investigated germination requirements of three perennial, endangered river corridor plants of Central European lowlands coexisting in subcontinental flood meadows, but preferring particular zones of decreasing flooding frequency and duration along the elevational gradient of the banks. It was hypothesized that the species have specific germination requirements to respond successfully to open patch creation depending on their occurrence along the gradient of spring flooding in the field. This study involved controlled experiments and phenological studies.     

Amount of calyx fibres in Lamiaceae, relation to calyx structure, phylogeny and ecology

Calyces of 306 species representing 181 out of the 236 genera of Lamiaceae have been studied with particular emphasis on the amount of fibres and similar xylem cells. A characteristic calyx tissue called ``mesophyll fibres', reported by earlier authors, is found to belong to the xylem. Most species of the subfamilies Lamioideae and Scutellarioideae differ from most other labiates, and particularly from Nepetoideae, in having much larger amounts of fibres and similar xylem cells in the calyx tube. This result supports cladistic hypotheses based on cpDNA showing that Lamioideae and Scutellarioideae are closely related and remotely related to Nepetoideae. The new data also contribute to the knowledge about the phylogeny within Lamioideae. The amount of fibres and similar cells also seems to be positively correlated with calyx size, calyx width, a ballistic dispersal mechanism in Scutellaria, and the aridity of the habitat. Possible adaptive significances of these correlations are discussed.     

Genetic variability in Plantago species in relation to their ecology

Summary A survey of enzyme variability in several populations of Plantago major in the Netherlands has been made. Nine of 36 loci were found to be polymorphic. The most extensively studies population showed 7 polymorphic loci (19%). The average heterozygosity was 0.005, a low value since P. major is predominantly inbreeding; a first estimate of the outcrossing rate is only 10%. All nine variable loci show simple Mendelian inheritance, seven of them could be placed into four different linkage groups. Marked differences in allele frequencies were found between two subspecies: ssp. major and ssp. pleiosperma. Two enzyme loci possess subspecies-specific alleles, Pgm-1 and Got-1. The most likely explanation of this phenomenon is the existence of fitness differences, caused either by the enzyme loci themselves or by linked loci.Grassland Species Research Group Publication no. 14     

Genetic variability in Plantago species in relation to their ecology

Summary Genetic variation in leaf and inflorescence morphology and in generative development within the species Plantago major has been analysed by means of crosses between members of two different subspecies. The variable characters chosen are supposed to be important for determining the ecological differences between the subspecies and other ecotypes. The analyses of F₂'s indicated that a substantial number of loci controlling the above mentioned characters are situated near the Pgm-1 locus, forming a gene complex. This gene complex can exist in at least three different forms in ssp. pleiosperma, ssp. major lawn type and ssp. major roadside type, respectively. In addition, some important factors for ecotypic differentiation are situated in the neighbourhood of the Got-1 locus and in a linkage group containing three other allozyme loci. These linkages between allozyme loci and fitness-affecting loci can explain the restriction of some enzyme alleles to a particular subspecies.Grassland Species Research Group Publication No. 50     

Genetic variability in Plantago species in relation to their ecology

Summary Twelve Plantago major plants, good representatives of their populations, appeared to be genetically different for several characters which are important for adaptation to the respective habitat conditions. These characters are: juvenile growth, leaf morphology, production of secondary rosettes, flowering time, seed production, seed size and adult leaf production. The adaptive value of some of these characters was investigated by transplantation experiments in the field and by intraspecific competition experiments. The roadside type of ssp. major was adapted to trampling by being erect and elastic. The lawn type of ssp. major was adapted to a short, frequently cut, vegetation by being prostrate and by producing leaves with short petioles throughout the growth season. In the natural situations in which ssp. pleiosperma occurs, growth rate and first-year seed production of this subspecies were considerably higher than that of ssp. major. In a number of experiments, F₁s and F₂s were included, derived from crosses between the original plants. The F₁s were generally rather well adapted to both parental habitats, whereas the F₂s appeared to be less fit. The various alternatives in spending resources relevant for fitness optimization in different habitats are discussed.Grassland Species Research Group Publication No. 93     

Genetic variability in Plantago species in relation to their ecology

Summary Populations of the three Plantago species P. major, P. lanceolata and P. coronopus were scored for allozyme variability. They showed normal variability levels compared with other, similar plant species. Differentiation among populations appeared to be rather low in comparison with other species, probably due to a considerable amount of long distance seed transport. In order to be able to make an analysis of small-scale gene flow, all (sub)populations were critically checked for the existence of population structure equilibrium. The allozyme variation was tested for neutrality by testing homogeneity of F values among loci: between populations (Lewontin-Krakauer test) and within populations. No systematic deviations from the prediction of the neutral theory could be established. From the population structure analyses, little gene flow in the species P. major (with high selfing levels) and P. coronopus could be concluded, whereas P. lanceolata showed relatively high levels of gene flow. The degree of homozygosity in the latter species was too high to explain with the available data. In P. coronopus, on the other hand, an unusually high number of heterozygotes were observed.Grassland Species Research Group Publication No. 73     

Diverging patterns of host use by phytophagous insects in relation to leaf pubescence in Arbutus xalapensis (Ericaceae)

Summary Foliar pubescence in the mexican madrone (Arbutus xalapensis H.B.K.) is an extremely variable character. Leaf specimens of pilose and glabrous madrones showed a similar composition of major secondary compounds. On the other hand, sympatric pilose and glabrous individuals were found to support significantly different insect guilds. Insect preference was strongly associated to mouthpart anatomy. Chewing and gall-forming insects were significantly more abundant on glabrous trees while sucking insects were more common on pilose trees. Additionally, parasitation of sucking insects was lower on pilose trees, possibly because the plant trichomes provide protection against parasitoids.     

The ecology of sewage treatment gradients in relation to their use by waterbirds

The distribution and abundance of waterbirds along sewage treatment gradients at the Western Treatment Plant (Victoria, Australia) were studied in late summer/early autumn 2000. In general, the highest densities and diversity of waterbirds, and of zooplankton, were found in the ponds towards the end of a treatment series. Filter-feeding waterfowl (Anatidae) probably used these ponds because of the availability of zooplankton as a food-source. Dissolved oxygen concentration generally increased along the treatment gradient and un-ionised sulphide concentration decreased, and it is possible that either one, or both, of these played a key role in determining the distribution of zooplankton.     

The optics of tsetse fly eyes in relation to their behaviour and ecology

Abstract The visual acuity of two species of tsetse flies, Glossina morsitans morsitans Westw. and Glossina pallidipes Aust., was investigated. Male G. morsitans eyes have an acute zone in the forward region, with large hexagonal lenses (mean minimum diameter, D=33, SE±0.7 μm), relatively small interommatidial angle (Δ(φ=1.08^o) and angular receptive field of individual ommatidia (Δp) of not less than 1.14^o. A narrow band of square lenses, with intermediate diameter and Δφ, merges with smaller hexagonal lenses in the periphery (24±0.7 μm), with relatively large interommatidial angle (Δφ=3.7^o) and small angular receptive field (Δp = c. 1.6^o). G.pallidipes eyes are similar, except that the lenses in the acute zone are larger than those of G.morsitans , in proportion to their larger body size. Female eyes are not significantly different from male eyes, except that they have a narrower region of binocular overlap (maximum for males = 24^o, for females = 18^o). The eye parameter (p=DΔφ) in the acute zone of male G.morsitans = 0.62, and in the peripheral zone = 1.56. These relatively high values are consistent with fast flight, visual detection of drift due to low wind speeds, mating chases and discrimination of cryptic host animals at high light intensities. The extended region of binocular overlap in males may serve as an early warning system of the approach of potential females. From our estimates, tsetse flies ought to be able to detect small objects against the sky c. 30 min before sunrise and after sunset, and to use their peripheral vision perhaps 15 min earlier and later than this.     

The magnesium content of some miliolacean foraminifera in relation to their ecology and classification

The mol.% magnesium carbonate is determined in eleven species belonging to nine genera of three miliolacean families. Samples of these species are from the North Queensland continental shelf adjacent to Townsville. Multiple analyses are done only on two species and these show a range from approximately 9 to 20 mol.% magnesium carbonate. All other species fell within this range. It is suggested that the MgCO₃ content in miliolacen foraminifera is not a reliable tool, for determining palaeotemperature, or palaeodepth, nor is it an aid to classification.     

Possibility of using hematopoietic stem cells in relation to their generation age

A hypothesis of the use of hemopoietic stem cells with a view of satisfying the demand for mature cells depending on their generation age predicts that CFUs that survived after repeated treatment with hydroxyurea should have a greater capacity for self-renewal. It has been demonstrated that after repeated administrations of hydroxyurea according to the scheme devised by the authors of the hypothesis (3-4 times) and as a result of a more prolonged treatment (6 times, every other 12 or 15 h), the capacity of the survived hemopoietic stem cells for self maintenance was not only lower than normal but commonly significantly decreased. The generation-age hypothesis of the use of hemopoietic stem cells thus remains badly needing experimental support.     

Slaves of the environment: the movement of herbivorous insects in relation to their ecology and genotype

The majority of insect species do not show an innate behavioural migration, but rather populations expand into favourable new habitats or contract away from unfavourable ones by random changes of spatial scale. Over the past 50 years, the scientific fascination with dramatic long-distance and directed mass migratory events has overshadowed the more universal mode of population movement, involving much smaller stochastic displacement during the lifetime of the insects concerned. This may be limiting our understanding of insect population dynamics. In the following synthesis, we provide an overview of how herbivorous insect movement is governed by both abiotic and biotic factors, making these animals essentially ''slaves of their environment''. No displaced insect or insect population can leave a resource patch, migrate and flourish, leaving descendants, unless suitable habitat and/or resources are reached during movement. This must have constrained insects over geological time, bringing about species-specific adaptation in behaviour and movements in relation to their environment at a micro- and macrogeographical scale. With insects that undergo long-range spatial displacements, e.g. aphids and locusts, there is presumably a selection against movement unless overruled by factors, such as density-dependent triggering, which cause certain genotypes within the population to migrate. However, for most insect species, spatial changes of scale and range expansion are much slower and may occur over a much longer time-scale, and are not innate (nor directed). Ecologists may say that all animals and plants are figuratively speaking ''slaves of their environments'', in the sense that their distribution is defined by their ecology and genotype. But in the case of insects, a vast number must perish daily, either out at sea or over other hostile habitats, having failed to find suitable resources and/or a habitat on which to feed and reproduce. Since many are blown by the vagaries of the wind, their chances of success are serendipitous in the extreme, especially over large distances. Hence, the strategies adopted by mass migratory species (innate pre-programmed flight behaviour, large population sizes and/or fast reproduction), which improve the chances that some of these individuals will succeed. We also emphasize the dearth of knowledge in the various interactions of insect movement and their environment, and describe how molecular markers (protein and DNA) may be used to examine the details of spatial scale over which movement occurs in relation to insect ecology and genotype.     

Biomass allocation in three macrophyte species in relation to the disturbance level of their habitat

1. The hypothesis was tested that perennial plants surviving in habitats frequently disturbed by floods should demonstrate the ability to escape the frequently scoured surficial zone by using refugia located deeper in the substrate. As a consequence, they should allocate more biomass to their underground parts than when growing in rarely disturbed habitats.
2. The allocation of biomass of three aquatic macrophytes ( Berula erecta , Groenlandia densa , Myriophyllum spicatum ) to their different organs (underground and above-ground parts) was measured in former river channels organized along a gradient of scouring flood disturbances.
3. For each species, biomass allocation to underground parts varied between sites: from 10.2 to 37.5% in B. erecta , from 14.9 to 31.1% in G. densa and from 8.4 to 23.2% in M. spicatum . It was correlated to the flood disturbance level of the site for B. erecta and M. spicatum but not for G. densa. No relationship was found between biomass allocation to underground parts and sediment richness.
4. The plasticity in biomass allocation of these three macrophyte species appears to be an adaptation to the variability in environmental conditions and implies difficulties in the classification of plant primary strategies, when this type of plasticity is ignored.     

Comparative seedling ecology of eight North American spruce (Picea) species in relation to their geographic ranges

BACKGROUND AND AIMS: Allowing for dispersal limitation, a species' geographic distribution should reflect its environmental requirements. Comparisons among closely related species should reveal adaptive differentiation in species characteristics that are consistent with their differences in geographic distribution. This expectation was tested by comparing characteristics of seedlings of spruce species in relation to environmental factors representative of their current natural ranges. METHODS: Seedlings were grown from a total of 34 populations representing eight North American spruce (Picea) species in a controlled environment chamber for 140 d. Traits related to the potential of seedling establishment, including tolerance to stress events (high temperature, desiccation) were evaluated. Correlations were sought between these characteristics and modal values of latitude, aridity and continentality in the geographic range of each species. KEY RESULTS: Many seedling traits changed significantly in response to stress events, but only the response of chlorophyll concentration differed significantly among species. Components of seedling growth were good correlates of species distribution. Seedling relative growth rate (RGR) and specific leaf area (SLA) were positively correlated with latitude, and leaf weight ratio (LWR) negatively correlated with aridity. Seed mass was negatively correlated with latitude. CONCLUSIONS: Relationships found between seedling traits and geographical variation in environmental conditions suggest that factors such as temperature regime, water availability and perhaps litter depth affect species range in North American spruces. Seedling characteristics appear to be elements in a reasonably distinct environmental niche for each spruce species at the continental scale.     

The arrangement of neurosecretory and catecholamine fibres in relation to the pituitary intermedia cells of the skate,Raja radiata

Summary The innervation pattern of the intermediate lobe of the skate (Raja radiata) was studied with histological and fluorescence histochemical methods. Neurosecretory fibres, stained with i.a. pseudo-iso-cyanine, were found running in bundles in the central parts of the cell cords. They terminated partly around the perinuclear parts of the intermedia cells, partly around the apices of the cells close to the vascular walls.A catecholamine innervation of the intermedia was also established. Catecholaminecontaining fibres with the appearance of nerve terminals were found around the intermedia cell apices close to the vessels. In some specimens, catecholamine fibres also seemed to terminate at the perinuclear parts of the cells.Thus it is possible, judging solely from structural relations, that both the cell body (the synthesis pole) and the cell apex (the release pole) receive a dual innervation. Recent experimental evidence indicates that the release of MSH from the pars intermedia is controlled by catecholamine fibres, but as yet there is only structural evidence for a special control of hormone synthesis.This study was supported by grants from the Swedish Natural Science Research Council (No. 99-35 and 2126-2) and was carried out within a research organization sponsored by the Swedish Medical Research Council (Projects No. B70-14X-712-05 and B70-14X-56-06).