共查询到20条相似文献,搜索用时 31 毫秒
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Valérie Pierard Allan Guiguen Laurence Colin Ga?lle Wijmeersch Caroline Vanhulle Beno?t Van Driessche Ann Dekoninck Jana Blazkova Christelle Cardona Makram Merimi Valérie Vierendeel Claire Calomme Thi Liên-Anh Nguyên Michèle Nuttinck Jean-Claude Twizere Richard Kettmann Daniel Portetelle Arsène Burny Ivan Hirsch Olivier Rohr Carine Van Lint 《The Journal of biological chemistry》2010,285(25):19434-19449
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Alexandra Sch?rg Sara Santambrogio James L. Platt Johannes Sch?del Maja T. Lindenmeyer Clemens D. Cohen Katrin Schr?dter David R. Mole Roland H. Wenger David Hoogewijs 《Nucleic acids research》2015,43(12):5810-5823
A crucial step in the cellular adaptation to oxygen deficiency is the binding of hypoxia-inducible factors (HIFs) to hypoxia response elements (HREs) of oxygen-regulated genes. Genome-wide HIF-1α/2α/β DNA-binding studies revealed that the majority of HREs reside distant to the promoter regions, but the function of these distal HREs has only been marginally studied in the genomic context. We used chromatin immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture (3C) to localize and functionally characterize a 82 kb upstream HRE that solely drives oxygen-regulated expression of the newly identified HIF target gene PAG1. PAG1, a transmembrane adaptor protein involved in Src signalling, was hypoxically induced in various cell lines and mouse tissues. ChIP and reporter gene assays demonstrated that the −82 kb HRE regulates PAG1, but not an equally distant gene further upstream, by direct interaction with HIF. Ablation of the consensus HRE motif abolished the hypoxic induction of PAG1 but not general oxygen signalling. 3C assays revealed that the −82 kb HRE physically associates with the PAG1 promoter region, independent of HIF-DNA interaction. These results demonstrate a constitutive interaction between the −82 kb HRE and the PAG1 promoter, suggesting a physiologically important rapid response to hypoxia. 相似文献
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Alice?E. Davidson Petra Liskova Cerys?J. Evans Lubica Dudakova Lenka Nosková Nikolas Pontikos Hana Hartmannová Kate?ina Hodaňová Viktor Stránecky Zbyněk Kozmík Hannah?J. Levis Nwamaka Idigo Noriaki Sasai Geoffrey?J. Maher James Bellingham Neyme Veli Neil?D. Ebenezer Michael?E. Cheetham Julie?T. Daniels Caroline?M.H. Thaung Katerina Jirsova Vincent Plagnol Martin Filipec Stanislav Kmoch Stephen?J. Tuft Alison?J. Hardcastle 《American journal of human genetics》2016,98(1):75-89