Influence of egg size differences within egg clutches on larval parameters in nine libellulid species (Odonata)

Abstract.  1. In libellulids, egg size differs between species and populations. There are also size differences within egg clutches of individual females.
2. Past experiments suggest that there are two different types of egg clutches in libellulids. Egg size decreases significantly during oviposition in species that perform non-contact guarding during oviposition. In contrast, in species ovipositing in tandem, egg size is randomly distributed.
3. This study deals with the possible consequences of egg size variation within the different egg clutch types. The study examined whether there is a correlation between egg development time, offspring sex or larval size and egg size.
4. The current experiments were conducted in Namibia and Germany. Five non-contact guarding and four tandem guarding libellulid species were used.
5. In some species larger eggs needed more time to develop, in some species no correlation between egg size and egg development time could be found, whereas in other species larger eggs developed faster.
6. The sex ratio was biased towards females in Leucorrhinia dubia and in Sympetrum striolatum and egg size was not associated with gender.
7. In both egg clutch types larger eggs resulted in larger larvae. In this study, evidence was found that the effects of egg size diminished with progressing larval development under good conditions. However, it is possible that the effects may have a greater influence under harsh circumstances.     

ON THE BIOLOGY OF THE MITE, GLYCYPHAGUS DOMESTICUS DE GEER. (TYROGLYPHIDAE, ACARINA)

1. A list is given of the mites most commonly found in houses. The most important of these is Glycyphagus domesticus , the furniture mite, and this has been mainly studied during this investigation.
2. An account is given of the occurrence of G. domesticus in houses and the conditions under which it is found.
3. The life history is described. It is characterised by the occurrence of the hypopus, a cyst-like stage which is found only amongst the Tyroglyphidae.
5. Humidity and temperature have a marked effect on the life of the mite. Tables are given showing the effect of ( a ) humidity on the adult, ( b ) humidity and temperature on the hypopus and egg.
6. Some experiments on the effect of carbon tetrachloride and methyl salicylate on the hypopus are recorded.     

AN INTRODUCTORY STUDY ON THE ECOLOGY OF HYRAX (DENDROHYRAX BRUCEI AND PROCAVIA JOHNSTONI) IN THE SERENGETI NATIONAL PARK

1. Dendrofoyrax brucei and Procavia johnstoni populations have been studied in kopjes surrounding the senior author's house.
2. The two species have been described and a short historical review of the Hyracoidea is given. Some notes are made concerning "Hyraceum".
3. The kopjes under study are described, being typical of granite outcrops in the Serengeti region.
4. The adaptations of both species of hyrax for the kopje habitat are described. Observations are made on the diurnal activity patterns in relation to climatic conditions.
5. A list of all plants available to the animals is presented, and those eaten by either species are indicated. Comment is made on the lack of overlap in the diets of the two species, and this is suggested as being partly due to lack of pressure on food resources.
6. A discussion on the possible evolution of the kopje-dwelling habit is held, which concludes that reconvergence may have taken place as a result of forest giving way to woodland and plains.     

Sib-mating and sex ratio strategies in scelionid wasps

Abstract. 1. Theoretical models by Hamilton and others predict that the optimal sex ratio (proportion males) for a species should decrease with increasing levels of sib-mating. A test of this hypothesis is made for members of the parasitoid family Scelionidae.
2. Scelionid wasps differ in levels of sib-mating as a consequence of differences in host egg mass size, male—male aggression and female—female aggression.
3. Sex ratios for thirty-one species vary with inferred changes in sib-mating as predicted by the models.
4. Interspecific differences in sex ratio may represent different fvted strategies or different manifestations of a single variable strategy (sex ratio game) in response to parasitoid-produced stimuli.
5. Males and females are assigned non-randomly to egg masses in a manner which ensures mixed broods in the proper proportions for a particular species.     

Intrinsic, inter-specific competition between egg, egg–larval, and larval parasitoids of plusiine loopers

Abstract.  1. Intrinsic, inter-specific competition between parasitoid wasp species is a key factor in ecological community dynamics and is particularly important for application in biological control. Here three parasitoid wasp species with overlapping host ranges and differing life history strategies were chosen to examine parasitoid–parasitoid interactions: the egg parasitoid Trichogramma pretiosum, the egg–larval, polyembryonic parasitoid wasp Copidosoma floridanum, and the gregarious larval parasitoid Glyptapanteles pallipes , with the plusiine loopers Acanthoplusia agnata and Trichoplusia ni as hosts.
2.  Copidosoma floridanum has been shown to be an intrinsically superior competitor against larval parasitoids because of their production and increased investment in a soldier larval caste during development, but little is known of their interactions with egg parasitoid species. Trichogramma pretiosum completely dominated intrinsic competition with C. floridanum regardless of oviposition order or sex of the C. floridanum egg.
3. Competition between C. floridanum and G. pallipes , however, depended on the host stage at which parasitism occurred, the sex of the C. floridanum egg, and parasitoid development time. Copidosoma floridanum outcompeted G. pallipes overall, despite the fact that G. pallipes injects a polyDNA virus into the host.
4. The sex of the C. floridanum egg was a significant factor in its ability to shift caste ratios to produce more soldiers in response to G. pallipes competition.
5. Only developing female C. floridanum responded to competition with G. pallipes by increasing the ratio of soldier to reproductive larvae, and this happened only when multiparasitism occurred in the host's 1st and 2nd instar.     

Density dependence and the augmentative release of egg parasitoids against graminaceous stalk borers

Abstract. 1. The augmentative release of Trichogranznza species for the biological control of graminaceous stalkborers is reviewed.
2. Previous experiments indicate strong density dependence acting on the early larval stages of the sorghum stalkborer ( Chilo partellus ). These data are described by a simple model for density dependence, whose properties are briefly described.
3. The effects upon the total mortality of different levels of egg parasitism are discussed when combined with density dependent larval losses that vary from under- to over-compensating.     

Yellow belly as honest signal of female quality in Knipowitschia panizzae(Gobiidae)

Sexually dimorphic traits are common in fish species, and examples from both males and females have been described. The function of these traits has been widely investigated in males. On the contrary, female ornaments have been studied mainly in sex role reversed species, such as pipefish, while their role in species with 'conventional' sex roles remain to be investigated.
This study focused on the presence, function, and possible role as indicator of female quality of a sexually dimorphic nuptial trait in the lagoon goby, Knipowitschia panizzae . In this species, that present conventional sex roles, females show a yellow spot on the belly. Aquarium spawning experiments demonstrated that the coloration on the belly is due to dermal pigments, is displayed only when female is ready to spawn and is switched off within few minutes from the end of egg deposition. This sexual trait presents variability in size among females and indicates female fecundity relatively to her own body size. As a consequence, female yellow belly appears to be an honest signal of female quality. Field data on natural nests highlighted that males perform parental cares mostly only on one egg batch at a time and the modality of egg deposition suggested that males are limited in their potential reproductive rates by environmental factors. Male limitation in egg care could constitute the basis for a female biased operational sex ratio, favouring male choosiness and the evolution of female nuptial displays.     

The effects of survival and weather on lifetime egg production in a model damselfly

Abstract. 1. A simulation model is presented which describes, from field-estimated parameters, the effects of daily survival rate and proportion of sunny days on the lifetime egg production of females of the damselfly Coenagrion puella (L).
2. Lifetime egg production increases with daily survival rate and proportion of sunny days.
3. Estimates of mean lifetime egg production per female in bad and good summers in northern England ranged from 333 to 740.
4. The distribution, as well as the proportion, of sunny days influences lifetime egg production. For a given combination of daily survival and proportion of sunny days, lifetime egg production decreases as the distribution of sunny days becomes more clumped.
5. Lifetime egg production is largely determined by chance; females who begin their mature adult life during a period of sunny weather can produce many times more eggs than those whose mature adult life coincides with cloudy days.     

Mating systems and sexual division of foraging effort affect puddling behaviour by butterflies

Abstract. 1. Foraging effort can vary among age classes and between the sexes. In many Lepidoptera, young males feed from mud, dung or carrion in a behaviour known as 'puddling', whereas females rarely puddle. In at least one species, males transfer sodium gained from puddling to females at mating for use in egg production.
2. Here we examine sex- and age-specific puddling patterns in seven montane butterfly species. We also test the hypothesis that among species in which young males predominate at puddles, differences in age- and sex-specific puddling patterns for a given species are related to mean female lifetime mating numbers.
3. For five species, young males fed proportionately more at puddles than other sex and age classes. Two species showed anomalous feeding patterns. In one, young females predominated at puddles; in the other, butterflies were rarely found at flowers.
4. As predicted, among the five species in which young males feed proportionately more at puddles, mean number of lifetime matings by females was negatively correlated with frequency of mud puddling by older females. A second prediction that mean number of lifetime matings by females is positively correlated with frequency of mud puddling by older males was not supported.
5. The results provide support for interspecific variation in division of responsibility between the sexes for resource acquisition for female reproduction, indicating close coordination between the sexes of foraging and life-history tactics.     

The consumption of eggs of the perch, Perca fluviatilis L., by macroinvertebrates in the field

SUMMARY. 1. Perch egg masses, in a late stage of development, were collected from a small stillwater and the composition of the egg masses and the number of invertebrates which colonized them was recorded.
2. All of the invertebrates that colonized the egg masses were tested serologically for the presence of perch eggs and the ability of some invertebrates to eat live and dead perch eggs in the laboratory was determined.
3. Of the nineteen taxa that colonized the egg masses, only two species of flatworm and three species of caddisfly larva ate perch eggs in the field and, of these, only one genus of caddisfly larva ate live eggs in the laboratory.
4. It is concluded that the invertebrates present were not important predators of perch eggs.     

FURTHER NOTES ON PAIRING AND SUBMISSIVE BEHAVIOUR OF THE RED-LEGGED PARTRIDGE ALECTORIS RUFA

1. The submissive display is described. It is sometimes given when the bird appears to be simultaneously frightened and attracted by a fellow member of the species.
2. The pairing behaviour of some individual captive birds is described. Some degree of mutual fear and hostility seems to be a necessary correlate of sexual attraction in this species. Birds that know and are at ease with one another do not pair.
3. A young male showed (in autumn) behaviour similar to that of a female ready to pair when introduced to an old and aggressive male. A possible biological function of such behaviour is suggested.     

Sex allocation and larval competition in a superparasitizing solitary egg parasitoid: competing strategies for an optimal sex ratio

1. Parasitic Hymenoptera reproduce by arrhenotokous parthenogenesis, and females of these species are able to control their progeny sex ratios. In structured populations of parasitic Hymenoptera, primary sex ratios are often highly biased toward females. However, sex ratio can be adjusted to the quality of encountered patches or hosts or be modified by differential developmental mortality.
2. In this paper, the effects were evaluated of the quality of encountered hosts and developmental mortality on the sex ratio in Anaphes victus , a solitary egg parasitoid whose first instar larvae present a sexual dimorphism and where superparasitism is regulated by larval fights between first instar larvae.
3. The results showed that a female-biased sex ratio is allocated to unparasitized hosts. In the presence of parasitized hosts, the second (superparasitizing) female produced a significantly higher sex ratio than the first female but the tertiary sex ratio (sex ratio at emergence) was not significantly different from the sex ratio produced with unparasitized hosts. The increase in the primary sex ratio produced by the second female was mostly compensated by the higher mortality of male larvae.     

The pay-off from superparasitism in the egg parasitoid Trissolcus basalis, in relation to patch defence

1. In species such as quasi-gregarious egg parasitoids, which exploit defendable patches, the fitness pay-off from superparasitism is an important factor in considering the evolution of patch defence and optimal patch defence strategies.
2. When oviposition in a previously parasitized host has a non-zero fitness pay-off, competing parasitoids are not only able to diminish the future value of a patch to other females by depleting unparasitized hosts, but also devalue the previous investment of other females in the patch by superparasitizing.
3. In Trissolcus basalis , an egg parasitoid of pentatomid bugs, the fitness pay-off from superparasitism is higher than 0.5 when the time interval between ovipositions is less than 3 h, suggesting that defending a previous investment will be an important component of patch defence behaviour.
4. The data suggest a mechanism for the high early pay-off, which involves the superparasitizing female adjusting the sex ratio of its offspring in favour of males. Males develop more quickly than females and thus may have a competitive advantage.     

Paternity analysis in the golden egg bug using AFLPs: do the males preferentially accept their true genetic offspring?

Abstract.  1. The evolution of parental care and intraspecific parasitism involve conflicts of interest between mothers and other potential care givers who contribute to enhance offspring survival. In the golden egg bug, Phyllomorpha laciniata Villers (Heteroptera: Coreidae), females lay eggs on conspecifics and on plants. The adaptive significance of egg carrying in this species has been the subject of some controversy, which can only be resolved by determining the genetic relationship between the eggs and the adult who carries them. This study examined whether male acceptance of true genetic offspring occurs with a higher frequency than that expected from random oviposition on conspecifics.
2. Paternity analysis, using Amplified Fragment Length Polymorphism (AFLP) markers, was conducted on eggs carried by males housed with field-mated females.
3. Out of the total number of eggs sired by males in the experimental groups, the proportion of eggs carried by males that were their true genetic offspring was 30.8%.
4. Monte Carlo methods show that the probability of a male accepting an egg that is his true genetic offspring is higher than expected if females dumped eggs on males at random.
5. These results suggest that paternal care plays an important role in the maintenance of male egg carrying in this species. In addition, the methodology developed may become useful in determining true genetic parents in other species in which neither the father nor the mother can be determined by observational methods.     

Sex-specific DNA in reptiles with temperature sex determination

Banded krait minor ("Bkm") satellite DNA, originating in the W-chromosome of the snake Bungarus fasciatus, has been found in the genome of diverse eukaryotic species including fruit fly, quail, and horse. Concentrations of Bkm have been found in the presumptive W-chromosome of snakes with isomorphic sex chromosomes and in the male-determining region of the Y-chromosome in mouse and man. We therefore asked whether Bkm-related DNA might be present in quantitative excess in DNA from males or females in two related species of sea turtle, Chelonia mydas, in which sex is determined by the temperature of the incubating egg, and Lepidochelys kempi, in which the critical sex-determining temperature has recently been described. Filter hybridization with the Bkm 2(8) probe revealed male-specific fragments in both species; female-specific fragments were also revealed in C. mydas. Sex-specific DNA sequences in temperature-sex-determined species such as Kemp's ridley and the green turtle were unexpected, but could be explained if there were an underlying genetic mode of sex determination in these animals, or alternatively, if temperature-influenced sex determination involved structural modifications in DNA adjacent to, or directly concerned with, the sex-determining genes. If these results are confirmed across a broader sample of sea turtles, the techniques described in this paper might be used routinely to identify gener in the young of these endangered animals, in which male and female are grossly indistinguishable.     

Frankliniella lantanae sp. n. (Thysanoptera: Thripidae), a polymorphic alien thrips damaging Lantana leaves in Australia

Abstract  A new species of Frankliniella Karny, probably introduced to Australia from the Caribbean area, is described here as causing feeding damage to the young leaves of Lantana in south-eastern Queensland and northern New South Wales. The new species is unusual within this large, primarily Neotropical genus, in that it feeds only on leaves, not in flowers, and that adult females are either apterous or fully winged. Only 10 of the 160 described species of Frankliniella are known to exhibit wing reduction, and notes are given on these together with notes on the seven species of the genus that are not endemic to the New World. Life history studies, including sex and morph ratios, indicate that egg to adult can require as little as 12 d, but despite breeding well in greenhouses, the species is not known to develop large populations in the field in Australia.     

BREEDING OF THE FULMAR FULMARUS GLACIALIS

.1. A study of individually marked Fulmars during three seasons has shown that both sexes can breed in successive years. The evidence for the theory of intermittent breeding is examined and definitions are given of the various categories of birds which compose the population during the breeding season. It is suggested that the supposed non-breeding adults which are seen, often in moult, in July are failed-breeders which have lost their egg or nestling.
2. No marked individual changed its nest-site between years, although the number and identity of sites occupied each July varied considerably.
3. No changes of mate occurred among the six marked pairs which nested in two consecutive years.
4. There is some evidence that both parents remain near the colony during the week before and after hatching.
5. Analysis of the 90 known sites of a colony subject to avian but not wild mammalian predation has revealed a wide range of habitat selection and a strong tendency toward close concentration of nests.
6. There is some evidence that the breeding season, as measured by date of hatching, varies from year to year.     

Grape variety affects female but also male reproductive success in wild European grapevine moths

Abstract.  1. For insect herbivores the quality of the larval host plant is a key determinant of their fitness. Only little attention, however, has been given to the effects of plants on mating success of males and its consequence for the reproductive output of their mates. In addition, almost all the studies that have investigated the influence of host plants on herbivore fitness components have been done in the laboratory, and less is known of these effects in natural conditions.
2. Using the phytophagous European grapevine moth ( Lobesia botrana Den. & Schiff., Lepidoptera: Tortricidae), we tested the influence of grape cultivars as larval food on the probability of acquiring a mate for both sexes, and on the reproductive output of females and males.
3. Results from this study stress the importance of larval host plants on the reproductive success of both sexes. Larval diet differentially affected mating success and reproductive output of male and female moths. Fecundity, egg size, and egg hatchability were significantly different when larvae were fed on particular grape cultivars.
4. A given cultivar that is of poor quality for females is generally also of poor quality for males. A cultivar, however, could be suitable for females but not for males and vice-versa. Apparently, the nutrients required for adult reproduction are not necessarily the same for males and females.
5. The important conclusion from this study is that evaluating the differential effect of host-plant species on traits associated with reproductive success of herbivores requires that the effects on both sexes be taken into account.     

Ecological correlates and heritability of reproductive variation in two co-occurring grasshopper species

ABSTRACT. 1. Relationships are examined between mean egg size and possible selection pressures acting on two grasshopper species, Chorthippus brunneus (Thunb.) and Myrmeleotettix maculatus (Thunb.), co-occurring at three sites in a sand dunes area.
2. Heavy eggs were laid where the mean crowding of hatchlings was high in relation to the amount of grasses with thin-edged leaves. To a lesser extent, large eggs were also found in more desiccating conditions. Mean egg dry weights did not correlate with measures of crowding or measures of crowding in relation to total grass abundance.
3. Significant differences in egg size and clutch sizes were observed between the stocks of second generation, laboratory-reared M.maculatus from three study sites. The significant difference in egg size (site 2 stock > site 1 stock) ran counter to the difference observed in the field but the difference in clutch size (site 3< site 2) was consistent with that observed between field populations.
4. We argue that egg size is primarily determined by selection pressures on the size of hatchlings but that predictably poor feeding conditions for juveniles and adults at site 2 results in the production of smaller adults which, in M.maculatus , then lay smaller eggs. We further argue that M. maculatus from site 2 have been selected to lay larger eggs for their body size and body condition in order to compensate partially for this environmental constraint.     

Reproductive variation and adult size in two co-occurring grasshopper species

ABSTRACT. 1. Egg sizes and clutch sizes of the grasshoppers Chorthippus brunneus (Thunb.) and Myrmeleotettix maculatus (Thunb.) were compared among three years and among three sites less than 1.3 km apart. Relationships between these reproductive traits and date of egg laying, body size and body condition were sought.
2. M.maculatus , the smaller species, laid fewer but larger eggs; and only the eggs of this species showed significant differences between sites and years.
3. A negative correlation between egg size and number per clutch was evident between species and years, but generally not among sites and among individuals of a population.
4. However, a hidden negative correlation between egg size and number was uncovered within populations when the relationship was examined for females of a given mature weight.
5. Variation in the number of eggs per clutch was explained statistically by a positive relationship between female body weight and egg number. Also, both interpopulation and intrapopulation comparisons revealed that for M.maculatus , but not for C.brunneus , females with long hind femurs laid large eggs.