共查询到20条相似文献,搜索用时 0 毫秒
1.
The sensitivity is the most important parameter in the sensing field. Effort was made to study the effect of gold coating on the sensitivity of rhombic silver nanostructure array through numerical simulation using the discrete dipole approximation method. This study shows that thickness of the gold coating can be varied to tune the sensitivity of the rhombic silver nanostructure array. The Au–Ag nanostructure array is found to possess the maximum refractive index sensitivity of 714 nm/RIU when thickness of gold is 20 nm, thickness of silver is 25 nm, and refractive index of the medium is around 1.35. The condition for achieving the maximum refractive index sensitivity can be used for detecting many species of biomolecules and drugs in the future. 相似文献
2.
Fernando Allan de Farias Rocha Bruno Duarte Gomes Luiz Carlos de Lima Silveira Sonia Limara Martins Renata Genaro Aguiar John Manuel de Souza Dora Fix Ventura 《PloS one》2016,11(1)
A new method is presented to determine the retinal spectral sensitivity function S(λ) using the electroretinogram (ERG). S(λ)s were assessed in three different species of myomorph rodents, Gerbils (Meriones unguiculatus), Wistar rats (Ratus norvegicus), and mice (Mus musculus). The method, called AC Constant Method, is based on a computerized automatic feedback system that adjusts light intensity to maintain a constant-response amplitude to a flickering stimulus throughout the spectrum, as it is scanned from 300 to 700 nm, and back. The results are presented as the reciprocal of the intensity at each wavelength required to maintain a constant peak to peak response amplitude. The resulting S(λ) had two peaks in all three rodent species, corresponding to ultraviolet and M cones, respectively: 359 nm and 511 nm for mice, 362 nm and 493 nm for gerbils, and 362 nm and 502 nm for rats. Results for mouse and gerbil were similar to literature reports of S(λ) functions obtained with other methods, confirming that the ERG associated to the AC Constant-Response Method was effective to obtain reliable S(λ) functions. In addition, due to its fast data collection time, the AC Constant Response Method has the advantage of keeping the eye in a constant light adapted state. 相似文献
3.
This paper deals with the effect of different size gold nanoparticles on the fluidity of lipid membrane at different regions of the bilayer. To investigate this, we have considered significantly large bilayer leaflets and incorporated only one nanoparticle each time, which was subjected to all atomistic molecular dynamics simulations. We have observed that, lipid molecules located near to the gold nanoparticle interact directly with it, which results in deformation of lipid structure and slower dynamics of lipid molecules. However, lipid molecules far away from the interaction site of the nanoparticle get perturbed, which gives rise to increase in local ordering of the lipid domains and decrease in fluidity. The bilayer thickness and area per head group in this region also get altered. Similar trend, but with different magnitude is also observed when different size nanoparticle interact with the bilayer. 相似文献
4.
Dong-Hua Yang Shinichiro Tsuyama Jun Ohmori Fusayoshi Murata 《The Histochemical journal》1998,30(9):687-692
Using bone marrow embedded in hydrophilic resin Lowicryl K4M and cationic colloidal gold pH 1.0 labelling, we studied sites of sulphation and sulphated glycosaminoglycans ultrastructurally in various maturational stages of both eosinophil granulocytes and eosinophil granules of guinea pig. Eosinophil granules reacted positively to cationic gold, the pattern of labelling varying according to the degree of cell maturation. The formation of eosinophil granules takes place throughout the myelocyte stage. Early eosinophil myelocytes contain a large Golgi apparatus with active granulogenesis, while late ones contain a small and less active Golgi apparatus. All the immature granules were labelled positively. However, mature granules with a central crystal bar lost their affinity towards colloidal gold. Interestingly, strong colloidal gold labelling was also observed in the trans to transmost Golgi apparatus, especially in immature eosinophil granulocytes. This indicates that sulphation of glycosaminoglycans occurs in the trans to transmost Golgi apparatus of eosinophil granulocytes. Prior absorption with poly-L-lysine prevented colloidal gold labelling of tissue sections. Methylation of sections at 37°C did not alter the gold labelling, whereas the labelling disappeared after methylation at 60°C. Prior treatment with chondroitinase ABC or heparinase I abolished the majority of colloidal gold labelling in immature eosinophil granules. Taking these results together, we conclude that immature eosinophil granules contain sulphated glycosaminoglycans including chondroitin sulphate or heparan sulphate or both. 相似文献
5.
Daniel Wangpraseurt Bojan Tamburic Milán Szabó David Suggett Peter J. Ralph Michael Kühl 《PloS one》2014,9(11)
The spectral light field of Symbiodinium within the tissue of the coral animal host can deviate strongly from the ambient light field on a coral reef and that of artificial light sources used in lab studies on coral photobiology. Here, we used a novel approach involving light microsensor measurements and a programmable light engine to reconstruct the spectral light field that Symbiodinium is exposed to inside the coral host and the light field of a conventional halogen lamp in a comparative study of Symbiodinium photobiology. We found that extracellular gross photosynthetic O2 evolution was unchanged under different spectral illumination, while the more red-weighted halogen lamp spectrum decreased PSII electron transport rates and there was a trend towards increased light-enhanced dark respiration rates under excess irradiance. The approach provided here allows for reconstructing and comparing intra-tissue coral light fields and other complex spectral compositions of incident irradiance. This novel combination of sensor technologies provides a framework to studying the influence of macro- and microscale optics on Symbiodinium photobiology with unprecedented spectral resolution. 相似文献
6.
Edward B. Seldin Richard H. White Paul K. Brown 《The Journal of general physiology》1972,59(4):415-420
The spectral sensitivity of lateral ocelli in both wild-type and white-eyed larvae of the yellow fever mosquito Aedes aegypti L. (reared in darkness) was measured by means of the electroretinogram. The spectral sensitivity is maximal at about 520 nm, with a small secondary peak near 370 nm. When allowance is made for some screening and filtering by the eye tissues, the spectral sensitivity is in reasonable agreement with the absorption spectrum of ocellar rhodopsin (λmax = 515 nm). 相似文献
7.
A microcalorimetric technique based on the bacterial heat output was explored to evaluate the effect of copper–indomethacin
complex on Staphylococcus aureus and Escherichia coli. The extent and duration of the inhibitory effect on the metabolism as judged from the rate constant (k) in log phase, half inhibitory ratio (IC50). The rate constant of bacteria in the presence of the drugs decreased with increasing concentrations of the drugs. The copper
complex exhibited higher antibacterial activity than the parent drug whose IC50 value was 1.5 and 2.3 times lower than that of indomethacin to S. aureus and E. coli, respectively. It was indicated that when the copper ion is coupled with indomethacin, the drug is more potent as a bacteriostatic. 相似文献
8.
Cryo-cooling is routinely performed before x-ray diffraction image collection to reduce the damage to crystals due to ionizing radiation. It has been suggested that although backbone structures are usually very similar between room temperature and cryo-temperature, cryo-cooling may hamper biologically relevant dynamics. In this study, the crystal of Escherichia coli dihydrofolate reductase is studied with replica-exchange molecular dynamics simulation, and the results are compared with the crystal structure determined at cryo-temperature and room temperature with the time-averaged ensemble method. Although temperature dependence of unit cell compaction and root mean-square fluctuation of Cα is found in accord with experiment, it is found that the protein structure at low temperature can be more heterogeneous than the ensemble of structures reported by using the time-averaged ensemble method, encouraging further development of the time-averaged ensemble method and indicating that data should be examined carefully to avoid overinterpretation of one average structure. 相似文献
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10.
Spectral Sensitivity of the Barnacle, Balanus amphitrite 总被引:1,自引:6,他引:1
The extracellular ocellar potential was used to evaluate the spectral sensitivity of the ocellus of the barnacle, Balanus amphitrite. Maximum relative sensitivity was at 530–540 nm. Studies with chromatic adapting lights suggest that the receptors contain a single photopigment. The spectra were relatively broader in the dark as compared to the light-adapted state. This effect was shown to be due to an increase in the slope of the amplitude-intensity function, caused by light adaptation. Studies of tapetal fluorescence and corneal transmission indicate little effect of the ocellar media on the determination of sensitivity. 相似文献
11.
Elasticity and Structure of Eukaryote Chromosomes Studied by Micromanipulation and Micropipette Aspiration 总被引:7,自引:2,他引:7
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Bahram Houchmandzadeh John F. Marko Didier Chatenay Albert Libchaber 《The Journal of cell biology》1997,139(1):1-12
The structure of mitotic chromosomes in cultured newt lung cells was investigated by a quantitative study of their deformability, using micropipettes. Metaphase chromosomes are highly extensible objects that return to their native shape after being stretched up to 10 times their normal length. Larger deformations of 10 to 100 times irreversibly and progressively transform the chromosomes into a “thin filament,” parts of which display a helical organization. Chromosomes break for elongations of the order of 100 times, at which time the applied force is around 100 nanonewtons. We have also observed that as mitosis proceeds from nuclear envelope breakdown to metaphase, the native chromosomes progressively become more flexible. (The elastic Young modulus drops from 5,000 ± 1,000 to 1,000 ± 200 Pa.) These observations and measurements are in agreement with a helix-hierarchy model of chromosome structure. Knowing the Young modulus allows us to estimate that the force exerted by the spindle on a newt chromosome at anaphase is roughly one nanonewton. 相似文献
12.
Nina Recek Xiaoqian Cheng Michael Keidar Uros Cvelbar Alenka Vesel Miran Mozetic Jonathan Sherman 《PloS one》2015,10(3)
The atomic force microscope (AFM) is broadly used to study the morphology of cells. The morphological characteristics and differences of the cell membrane between normal human astrocytes and glial tumor cells are not well explored. Following treatment with cold atmospheric plasma, evaluation of the selective effect of plasma on cell viability of tumor cells is poorly understood and requires further evaluation. Using AFM we imaged morphology of glial cells before and after cold atmospheric plasma treatment. To look more closely at the effect of plasma on cell membrane, high resolution imaging was used. We report the differences between normal human astrocytes and human glioblastoma cells by considering the membrane surface details. Our data, obtained for the first time on these cells using atomic force microscopy, argue for an architectural feature on the cell membrane, i.e. brush layers, different in normal human astrocytes as compared to glioblastoma cells. The brush layer disappears from the cell membrane surface of normal E6/E7 cells and is maintained in the glioblastoma U87 cells after plasma treatment. 相似文献
13.
Plasmonics - A sensitive spectral sensing method for detection of alpha-fetoprotein (AFP) was proposed based on the intense aggregation of colloidal gold nanostars. It has been found that the... 相似文献
14.
Cytoplasmic Solvent Structure of Single Barnacle Muscle Cells Studied by Electron Spin Resonance 总被引:3,自引:0,他引:3
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A free radical probe was introduced into single barnacle muscle cells, and its freedom of motion inferred from the spin resonance spectra. The probe reported an average local viscosity of 5-10 cp compared with 1 cp for pure water. From a comparison of the temperature dependence of the probe's tumbling rate in model aqueous systems and in the muscle we concluded that in the muscle the probe was undergoing fast exchange between sites of different mobility. Thus 10 cp must be taken as an upper limit for the viscosity of most cell water. 相似文献
15.
We report the observation of focusing and deflection phenomena by employing a novel technique to perform phase front profile design in nanoslit-based planar plasmonic lenses and beam deflectors. Introducing perpendicular cuts and bumps to the perforated nanoslits on a thin metallic film is utilized to change the effective depth of the nanoslits which provide the possibility of manipulating the phase front profile based on the propagation property of the surface plasmon polaritons in the metal–insulator–metal waveguides. Using the dispersive finite-difference time-domain numerical method, simulations are conducted to explore the beam focusing and deflection phenomena, and the performance parameters of the lens and beam deflector include the focal length, full-width half-maximum, depth of focus, the efficiency of focusing, and the deflection angle. The whole structure is formed on a planar thin film which is convenient for miniaturization and high density integration besides that it can be fabricated by well-known techniques such as focused ion beam milling. 相似文献
16.
A method was developed to investigate the mechanical structure of the cytoplasm based on the movement of an intracellular gold particle subjected to centrifugal acceleration (the gold particle method). The movement of the particle in the cell was observed and recorded with a new centrifuge microscope of stroboscopic type (13). In eggs and oocytes of the echinoderms, Clypeaster japonicus, Asterias amurensis , and Asterina pectinifera , the particle moved in the cytoplasm by an applied centrifugal acceleration in the centrifugal direction, but the course was not exactly straight and the velocity fluctuated during the movement, suggesting the existence of a network structure in the cytoplasm. In fertilized eggs, the movement of the particle by the centrifugal acceleration was impeded by the structures of the sperm aster and the cleavage diaster. The apparent viscosity of the cytoplasm in fertilized eggs changed in parallel to the development of the sperm aster and the mitotic diaster in the cell. These results indicate that the asters are really rigid structures in the cell as previously shown by the magnetic particle method (8). 相似文献
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18.
Špačková Barbora Lebrušková Petra Šípová Hana Kwiecien Pavel Richter Ivan Homola Jiří 《Plasmonics (Norwell, Mass.)》2014,9(4):729-735
We investigate the optical response to refractive index changes of a Fano resonance occurring in a random array of gold nanoparticles supported on a glass substrate. The Fano resonance results from the interference between localized surface plasmon on a gold nanoparticle and the light reflected at the boundary of the glass substrate. We demonstrate that the sensitivity of the resonance to the refractive index of the surrounding medium is highly dependent on the excitation geometry and can assume either positive or negative values. We furthermore present a theoretical analysis explaining this behavior based on the rigorous coupled wave analysis (RCWA) as well as the island film theory.
相似文献19.
Adequate digital resolution and signal sensitivity are two critical factors for protein structure determinations by solution NMR spectroscopy. The prime objective for obtaining high digital resolution is to resolve peak overlap, especially in NOESY spectra with thousands of signals where the signal analysis needs to be performed on a large scale. Achieving maximum digital resolution is usually limited by the practically available measurement time. We developed a method utilizing non-uniform sampling for balancing digital resolution and signal sensitivity, and performed a large-scale analysis of the effect of the digital resolution on the accuracy of the resulting protein structures. Structure calculations were performed as a function of digital resolution for about 400 proteins with molecular sizes ranging between 5 and 33 kDa. The structural accuracy was assessed by atomic coordinate RMSD values from the reference structures of the proteins. In addition, we monitored also the number of assigned NOESY cross peaks, the average signal sensitivity, and the chemical shift spectral overlap. We show that high resolution is equally important for proteins of every molecular size. The chemical shift spectral overlap depends strongly on the corresponding spectral digital resolution. Thus, knowing the extent of overlap can be a predictor of the resulting structural accuracy. Our results show that for every molecular size a minimal digital resolution, corresponding to the natural linewidth, needs to be achieved for obtaining the highest accuracy possible for the given protein size using state-of-the-art automated NOESY assignment and structure calculation methods. 相似文献
20.
C. W. Hilbers A. Heerschap C. A.G. Haasnoot J. A.L.I. Walters 《Journal of biomolecular structure & dynamics》2013,31(1):183-207
Abstract Recently, the imino proton spectrum of yeast tRNAPhe has been assigned by means of the application of the nuclear Overhauser effect (NOE). In the present paper it will be shown that even for tRNA (MW 28000) connectivities between the imino proton spins can be observed using two-dimensional NOE spectroscopy. In this way the imino proton resonances of the D-stem region are assigned. The results are discussed in relation to those obtained by the classical one-dimensional nuclear Overhauser effect. It turns out that in 2D-NOE experiments connectivities from overlapping resonances can be observed which cannot be determined by one-dimensional Overhauser experiments. Moreover, the total assignment of the imino proton spectrum of yeast tRNAPhe is used to relate the three-dimensional crystal structure of the tRNA to its solution structure. It is shown that the principle elements of the X-ray structure, i.e. the hydrogen bonding network and the stacking of the stems upon one another, are also found in solution. This is true for the presence as well as for the absence of magnesium ions. However, in absence of magnesium ions the tRNA structure appears to differ in details from that in the presence of magnesium ions. Finally, the influence of the elongation factor Tu from B.stearothermophilus on the tRNA structure is discussed. 相似文献