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1.
Radioactive polysaccharide was synthesized when uridine 5′-(α-d-[U-14C]apio-d-furanosyl pyrophosphate) (containing some uridine 5′-(α-d-[U-14C]xylopyranosyl pyrophosphate)) was incubated with a particulate enzyme preparation from Lemna minor. Characterization experiments established that the product: (i) was insoluble in methanol and water, (ii) contained d-[U-14C]apiose (75%) and d-[U-14C]xylose (25%), and (iii) was soluble in 1% ammonium oxalate. The material solubilized by ammonium oxalate (solubilized product): (i) was separated into five fractions by column chromatography with diethylaminoethyl-Sephadex (DEAE-Sephadex), (ii) contained [U-14C]apiobiose side chains that were removed by hydrolysis at pH 4, and (iii) was degraded by fungal pectinase. Both d-[U-14C]apiose residues of the [U-14C]apiobiose side chains were synthesized in vivo since radioactivity was distributed equally between the two residues. The presence of uridine 5′-(α-d-galactopyranosyluronic acid pyrophosphate) during synthesis of radioactive polysaccharide resulted in: (i) an increase in the incorporation of radioactive d-[U-14C]apiose into solubilized product, (ii) an increase in the ratio of d-[U-14C]apiose to d-[U-14C]xylose present in solubilized product, (iii) an increase in the amount of [U-14C]apiobiose plus d-[U-14C]apiose released from the solubilized product by hydrolysis at pH 4, and (iv) a tighter binding of the solubilized product to DEAE-Sephadex. These results show that apiogalacturonans similar to or the same as those synthesized by the intact plant were synthesized in the particulate enzyme preparation isolated from L. minor. [14C]Apiogalacturonans completely free of d-[U-l4C]xylose were not isolated. The [14C]apiogalacturonan with the least d-[U-14C]xylose still had 4.8% of its radioactivity present in d-[U-14C]xylose. The possibility remains that d-xylose is a normal constituent of the apiogalacturonans of the cell wall of L. minor. 相似文献
2.
Synthesis, characterization and properties of uridine 5′-(α-d-apio-d-furanosyl pyrophosphate) 总被引:1,自引:0,他引:1
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1. A method was developed for synthesizing UDP-apiose [uridine 5'-(alpha-d-apio-d-furanosyl pyrophosphate)] from UDP-glucuronic acid [uridine 5'-(alpha-d-glucopyranosyluronic acid pyrophosphate)] in 62% yield with the enzyme UDP-glucuronic acid cyclase. 2. UDP-apiose had the same mobility as uridine 5'-(alpha-d-xylopyranosyl pyrophosphate) when chromatographed on paper and when subjected to paper electrophoresis at pH5.8. When [(3)H]UDP-[U-(14)C]glucuronic acid was used as the substrate for UDP-glucuronic acid cyclase, the (3)H/(14)C ratio in the reaction product was that expected if d-apiose remained attached to the uridine. In separate experiments doubly labelled reaction product was: (a) hydrolysed at pH2 and 100 degrees C for 15min; (b) degraded at pH8.0 and 100 degrees C for 3min; (c) used as a substrate in the enzymic synthesis of [(14)C]apiin. In each type of experiment the reaction products were isolated and identified and were found to be those expected if [(3)H]UDP-[U-(14)C]apiose was the starting compound. 3. Chemical characterization established that the product containing d-[U-(14)C]apiose and phosphate formed on alkaline degradation of UDP-[U-(14)C]apiose was alpha-d-[U-(14)C]apio-d-furanosyl 1:2-cyclic phosphate. 4. Chemical characterization also established that the product containing d-[U-(14)C]apiose and phosphate formed on acid hydrolysis of alpha-d-[U-(14)C]apio-d-furanosyl 1:2-cyclic phosphate was d-[U-(14)C]apiose 2-phosphate. 5. The half-life periods for the degradation of UDP-[U-(14)C]apiose to alpha-d-[U-(14)C]apio-d-furanosyl 1:2-cyclic phosphate and UMP at pH8.0 and 80 degrees C, at pH8.0 and 25 degrees C and at pH8.0 and 4 degrees C were 31.6s, 97.2min and 16.5h respectively. The half-life period for the hydrolysis of UDP-[U-(14)C]-apiose to d-[U-(14)C]apiose and UDP at pH3.0 and 40 degrees C was 4.67min. After 20 days at pH6.2-6.6 and 4 degrees C, 17% of the starting UDP-[U-(14)C]apiose was degraded to alpha-d-[U-(14)C]apio-d-furanosyl 1:2-cyclic phosphate and UMP and 23% was hydrolysed to d-[U-(14)C]apiose and UDP. After 120 days at pH6.4 and -20 degrees C 2% of the starting UDP-[U-(14)C]apiose was degraded and 4% was hydrolysed. 相似文献
3.
A simple, three-step conversion of 1,2-O-isopropylidene-α-d-glucofuranose into l-ascorbic acid, originally described by Bakke and Theander, was used to prepare l-[4-14C]ascorbic acid from milligram amounts of d-[3-14C]glucopyranose in 28% radioisotopic yield. In addition, l-[6-14C]- and l-[U-14C]-ascorbic acid were prepared from d-[1-14C]- and d-[U-14C]-glucopyranose, respectively. The procedure is useful for the synthesis of l-ascorbic acid bearing isotopic hydrogen, carbon, or oxygen atoms at specific positions, subject only to the availability of starting material. 相似文献
4.
A quantitative triterpene analysis was made of latex stem tissue of Euphorbia lathyris. Young plants seedlings of E. lathyris were incubated with various labelled precursors. Incorporation into triterpenes was obtained from [2-14C]mevalonic acid, [1-14C]acetate, [3-14C]pyruvate, [U-14C]sucrose, [U-14C]glucose, [U-14C]xylose, [U-14C]glyoxylate, [2,3-14C]succinic acid, [1-14C]glycerol [U-14C]serine. Both sugars tyrosine appeared to be effective precursors in DOPA synthesis inside the laticifers. Exogenously supplied mevalonic acid was only involved in triterpene synthesis outside the laticifers. GC-RC of triterpenes synthesized from [U-14C]glucose revealed the origin of these compounds in the latex. The labelled triterpenes obtained after incorporation of the other mentioned labelled precursors were only partly synthesized in the laticifers. For quantitative data on latex triterpene synthesis seedlings were incubated with [U-14C]sucrose, [U-14C]glucose, [U-14C]xylose [1-14C]acetate in the presence of increasing amounts of unlabelled substrate. From the amount of 14C incorporated into the triterpenes the amount of substrate directly involved in triterpene synthesis was calculated, as was the absolute triterpene yield. Sucrose showed the highest triterpene yield, equivalent to the daily increase of the triterpene content of growing seedlings. The possible significance of the other precursors in triterpene synthesis in the laticifers is discussed. 相似文献
5.
Mature seeds of Tropaeolum majus L. contain the cell wall polysaccharide xyloglucan (amyloid), protein and lipid as storage substances. The transitory occurrence of starch during the process of seed development could be substantiated.[U-14C]-labelled xylose, glucose and glucuronic acid were fed to ripening seeds and the incorporation of radioactivity into xyloglucan, starch and the sugar nucleotide fraction of the cotyledons was determined. The results indicate that exogenous supplied xylose is not incorporated directly into xyloglucan, but is transformed to glucose before incorporation into xyloglucan and starch. Radioactivity from glucuronic acid was predominantly found in the xylose moiety of xyloglucan. Incubation of seeds with [6-14C]-labelled glucose resulted in an incorporation of labelled hexoses into amyloid and starch, whereas xylose residues of amyloid remained unlabelled.Abbreviations p.a.
post anthesis
- UDP
uridine 5-diphosphate
- GDP
guanosine 5-diphosphate
- TLC
thin layer chromatography
- HPLC
high pressure liquid chromatography 相似文献
6.
Fluoride-Induced Inhibition of Starch Biosynthesis in Developing Potato, Solanum tuberosum L., Tubers Is Associated with Pyrophosphate Accumulation 总被引:1,自引:1,他引:0
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Pretreatment of discs excised from developing tubers of potato (Solanum tuberosum L.) with 10 millimolar sodium fluoride induced a transient increase in 3-phosphoglycerate content. This was followed by increases in triose-phosphate, fructose 1,6-bisphosphate and hexose-phosphate (glucose 6-phosphate + fructose 6-phosphate + glucose 1-phosphate). The effect of fluoride is attributed to an inhibition of glycolysis and a stimulation of triose-phosphate recycling (the latter confirmed by the pattern of 13C-labeling [NMR] in sucrose when tissue was supplied with [2-13C]glucose). Fluoride inhibited the incorporation of [U-14C] glucose, [U-14C]sucrose, [U-14C]glucose 1-phosphate, and [U-14C] glycerol into starch. The incorporation of [U-14C]ADPglucose was unaffected. Inhibition of starch biosynthesis was accompanied by an almost proportional increase in the incorporation of 14C into sucrose. The inhibition of starch synthesis was accompanied by a 10-fold increase in tissue pyrophosphate (PPi) content. Although the subcellular localization of PPi was not determined, a hypothesis is presented that argues that the PPi accumulates in the amyloplast due to inhibition of alkaline inorganic pyrophosphatase by fluoride ions. 相似文献
7.
M J Pitts M Chemielewski M S Chen M M el-Rahman R L Whistler 《Archives of biochemistry and biophysics》1975,169(2):384-391
5-Thio-α-d-[U-14C]glucopyranose and 6-thio-β-d-[U-14C]fructopyranose were administered orally and intravenously to rats. On intravenous administration of 5-thio-d-[U-14C]glucopyranose, 1% was oxidized to [14C]carbon dioxide, 93% was excreted in the urine, and 1.6% was retained in the carcass. On oral administration of 5-thio-d-[U-14C]glucopyranose, 1% was exhaled as [14C]carbon dioxide, 90% was excreted in feces and urine, and 4% was retained in the carcass after 72 h. On intravenous administration of 6-thio-β-d-[U-14C]fructopyranose, 56% was exhaled as [14C]carbon dioxide, 23% was excreted in the urine, and 7.5% was retained in the carcass; after oral administration, 35% was oxidized to [14C]carbon dioxide, 50% was excreted in feces and urine, and 6% was retained in the animal after 72 h.On intravenous administration of 5-thio-d-glucose to fasted male rats, blood d-glucose levels increased at lower doses than on oral administration. A dose of 50 mg/kg raised blood d-glucose to 226 mg/100 ml within 2.5 h after intravenous but only to 173 mg/100 ml within 2 h after oral administration from basal level of 70–90 mg/100 ml. Blood d-glucose concentration returned to normal levels within 9 h in both cases. 6-Thio-d-fructopyranose showed no diabetogenic action. The LD50 of 6-thio-d-fructopyranose was 11,200 mg/kg when tested in mice. 相似文献
8.
Biosynthesis of securinine was studied by incorporation experiments in Securinega suffruticosa. Among presumed precursors tested, lysine, cadaverine, and tyrosine showed the highest incorporation into securinine. Degradation experiments revealed that cadaverine-[1,5-14C] labelled specifically the piperidine ring of securinine and the radioactivity from dl-tyrosine-[2-14C] was introduced into the C-11 lactone carbonyl. Experiments with L-tyrosine-[U-14C] and L-tyrosine-[3′,5′-3H; U-14C] prove that the remaining C6Sz.sbnd;C2 moiety is derived from the aromatic ring and the C-2 and C-3 or tyrosine. 相似文献
9.
BURT GOLDBERG DONNA RATTENDI NIGEL YARLETT DAVID LLOYD CYRUS J. BACCHI 《The Journal of eukaryotic microbiology》1997,44(4):352-358
ABSTRACT. The fate of methionine in eukaryotic cells is divided between protein synthesis and the branched pathway encompassing polyamine synthesis, methylation of proteins and lipids, and transsulphuration reactions. Aside from protein synthesis, the first step to all other uses of methionine is conversion to S-adenosylmethionine. Blockade of polyamine synthesis in African trypanosomes by the ornithine decarboxylase inhibitor DL-α-difluoromethylomithine (Ornidyl, DFMO) the AdoMet decarboxylase inhibitor 5′-{[(Z)-4-amino-2-butenyl]-methylamino}-5′-deoxyadenosine or the protein methylase inhibitor sinefungin induces dramatic increases in intracellular AdoMet. In a previous study, distribution and pool sizes of [15S] or [U-14C]methionine were followed in bloodform trypanosomes as incorporation into the total TCA precipitable fraction. In the present study, the effects of pretreatment with DFMO (1 mM), MDL 73811 (1 μM) and sinefugin (2 nM) on [35S] and [U-14C]methionine incorporation were studied in blood forms. DFMO or MDL 73811 pretreatment increased protein methylation 1.5-fold through incorporation of [U14C]methionine, while sinefungin caused a 40% reduction of incorporation. The increases in incorporation of [U-14C]methionine due to DFMO and MDL 73811 were reduced 40% to 70% by including cold AdoMet (1 mM) in the incubation medium, an indication of AdoMet transport by bloodform trypanosomes and the utilization of [U-14C]methionine as AdoMet. Exogenous AdoMet had no effect on [35S]methionine incorporation. The agents studied are curative for African trypnosomiasis infections, either clinically (DFMO) or in model infections (MDL 73811, sinefungin) and thus highlight interference with AdoMet metabolism and methylation reactions as biochemical consequences of these agents. 相似文献
10.
Saito K 《Plant physiology》1978,62(2):215-219
[U-14C]Sucrose, myo-[U-14C]inositol, [6-14C]- and [U-14C]glucuronate, UDP-[U-14C]glucuronate, [U-14C]gluconate, and l-[1-14C]ascorbic acid were fed into grape berries, Vitis labrusca L. cv. Delaware, at intervals throughout the ripening process and incorporation of 14C into several metabolites was studied. 相似文献
11.
Biosynthesis of the fucose-containing xyloglucan nonasaccharide by pea microsomal membranes 总被引:4,自引:3,他引:1
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Pea microsomal membranes catalyze the transfer of [14C]fucose (Fuc) from GDP-[U-14C]fucose, with or without added unlabeled UDP-glucose (Glc), UDP-xylose (Xyl) or UDP-galactose (Gal), to an insoluble product with properties characteristic of xyloglucan. After digestion of the ethanol-insoluble pellet with Streptomyces griseus endocellulase, [14C] fucose residues occur exclusively in a fragment corresponding in size to the xyloglucan nonasaccharide, Glc4 Xyl3 Gal Fuc. This fragment contains a single labeled fucose residue per oligomer, α-linked in a terminal nonreducing position. By comparison, in incubations where GDP-[14C] fucose is absent and replaced by UDP-[3H]xylose, the maximum size of labeled oligosaccharide found following cellulase digestion of products is an octasaccharide. In the presence of both GDP-[14C]fucose and UDP-[3H]xylose, a nonasaccharide containing the two labels is produced. Fucose and xylose residues are transferred within a few minutes to acceptor molecules of molecular weight up to 300,000. Such products do not elongate detectably over 60 minutes of incubation. The data support the conclusion that the nonasaccharide subunit of xyloglucan may be generated in vitro by transfucosylation to preformed acceptor chains, and that its synthesis is dependent on the inclusion of exogenous GDP-fucose. 相似文献
12.
The lipids of Caldariella acidophila, an extreme thermophile member of the new archaebacteria group, are macrocyclic tetraethers. They are made up of two glycerol molecules (or one glycerol and one nonitol) bridged through ether linkages by two C4016,16′-biphytanyl chains. To elucidate the biosynthesis of the glycerol moiety of these tetraethers and the mechanism of glycerol ether assembly, labelled [U-14C, 1(3)-3H]glycerol and [U-14C, 2-3H]glycerol, were fed to C. acidophila. Both precursors were selectively incorporated with high efficiency, and without any change in the 3H/14C ratio, in the glycerol moiety of tetraethers. These results suggest that the ether forming step in the biosynthesis of tetraether lipids of C. acidophila, occurs without any loss of hydrogen from any of the glycerol carbons which in turn could be directly alkylated by geranylgeranyl pyrophosphate. The incorporation of radioactivity in the isoprenoid chains and into nonitol is also analysed. 相似文献
13.
A particulate enzyme preparation isolated from Chrysochromulina chiton catalysed the transfer of [U-14C]-glucose from UDP [U-14C]-Glc to a water-soluble small molecular weight material. Chemical and enzymic analysis of this material showed that it was a phenolic compound to which are attached two β(1–3) glucosides. Properties of the UDP glucose: glucosyltransferase involved in the synthesis of this material have been studied. The UDP glucose glucosyl-transferase was found to be associated with the rough endoplasmic reticulum. A possible function of this phenolic compound in the orientation of membranes for the synthesis of scales in C. chiton has been discussed. 相似文献
14.
J E Cronan 《Analytical biochemistry》1980,103(2):377-380
β-[U-14C]Alanine can be synthesized in >95% yield from l-[U-14C]aspartic acid using the aspartate 1-decarboxylase of Escherichia coli and converted to d-[1,2,3-14C]pantothenate in a 10–20% yield using the pantothenate synthetase of E. coli. Sufficiently pure preparations of both enzymes are readily obtained. 相似文献
15.
Feeding experiments have shown that 2′-7-dihydroxy-4′-methoxy-isoflavone-[Me-14C] and -isoflavanone-[Me-14C] are efficient precursors of the phytoalexins demethylhomopterocarpin, sativan and vesitol in CuCl2-treated lucerne (Medicago sativa) seedlings. Demethylhomopterocarpin-[Me-14C] was also incorporated into sativan and vestitol, and vestitol-[Me-14C] was incorporated into demethylhomopterocarpin and sativan. Thus, the pterocarpan demethylhomopterocarpin and the 2′-hydroxy-isoflavan vestitol are interconvertible in M. sativa, but incorporation data, and the results of kinetic feeding experiments with l-phenylalanine-[U-14C] suggest that these compounds are synthesized simultaneously from a common intermediate, which could be involved in the interconversion. A carbonium ion, derived from an isoflavanol, a likely intermediate in the biosynthetic reductive sequence from 2′,7-dihydroxy-4′-methoxy-isoflavone and -isoflavanone, is proposed as this common intermediate. 7-Hydroxy-2′,4′-dimethoxyisoflavone-[4′-Me-14C] was a very poor precursor of all three phytoalexins. Sativan, then, is most probably derived by methylation of vestitol. The incorporation of vestitol-[Me-14C] into demethylhomopterocarpin, but not into maackiain, pterocarpan phytoalexins of red clover (Trifolium pratense), is also demonstrated. 相似文献
16.
Robin E. Mitchell 《Phytochemistry》1985,24(2):247-249
Addition of either l-[U-14C]threonine or l-[U-14C]isoleucine to 2.7-day-old shaking liquid cultures of Pseudomonas syringae pv. atropurpurea resulted in incorporation of radioactivity into coronatine, but not into N- coronafacoylvaline, another phytotoxin excreted by P.s. atropurpurea. In contrast, addition ofl-[U-14C]valine did not lead to incorporation of radioactivity into coronatine, but instead into coronafacoylvaline. Acid hydrolysis of the purified [14C] coronatine obtained after incorporation of either [14C]isoleucine or [14C]threonine demonstrated that > 94% of the radioactivity was present in the 1-amido-1-carboxy-2-ethylcyclopropyl moiety of coronatine, and < 6 % was in the coronafacoyl moiety. These findings are used to propose a biosynthetic pathway for coronatine. 相似文献
17.
Paul M. Dewick 《Phytochemistry》1975,14(4):983-988
Feeding experiments have demonstrated the specific incorporation of radioactivity from dl-phenylalanine-[1-14C], l-phenylalanine-[U-14C], sodium acetate-[2-14C] and l-methionine-[methyl-14C] into the 3-benzylchroman-4-one eucomin in Eucomis bicolor. The labelling patterns indicate that eucomin is biosynthesized by the addition of a carbon atom derived from methionine onto a C15 chalcone-type skeleton. Radioactivity from 2′,4′,4-trihydroxy-6′-methoxychalcone-[methyl-14C] and 2′,4′-dihydroxy-4,6′-dimethoxychalcone-[6′-methyl-14C] was incorporated into eucomin, the latter compound being the better precursor, demonstrating the feasibility that 2′-methoxychalcones are biosynthetic precursors of the “homoisoflavonoids”. Possible biosynthetic relationships in this class of compounds are discussed. 相似文献
18.
《Neurochemistry international》1986,8(1):67-75
The metabolism of oligodendrocytes has been studied using cultures of oligodendrocyte-enriched glial cells isolated from cerebra of 5–8-day old rats. Cultures containing 60–80% oligodendrocytes were incubated for 16h with [3-14C]acetoacetate, d-[3-14C]3-hydroxybutyrate, [U-14C]glucose, l-[U-14C]glutamine and [1-14C]pyruvate or [2-14C]pyruvate in the presence or absence of other oxidizable substrates. Labelled CO2 was collected as an index of oxidative metabolism and the incorporation of label into total lipids, fatty acids and cholesterol was used as an index of the de novo synthesis of lipids. Glucose, acetoacetate, D-3-hydroxybutyrate, pyruvate and l-lactate were measured to determine substrate utilization and product formation under various conditions. Our results indicate that glucose is rapidly converted to lactate and is a relatively poor substrate for oxidative metabolism and lipid synthesis. Ketone bodies were used as an energy source and as precursors for the synthesis of fatty acids and cholesterol. Preferential incorporation of acetoacetate into cholesterol was not observed. Exogenous pyruvate was incorporated into both the glycerol skeleton of complex lipids and into cholesterol and fatty acids. l-Glutamine appeared to be an important substrate for the energy metabolism of these cells. 相似文献
19.
DEVELOPMENT OF LIPOGENESIS IN RAT BRAIN CORTEX: THE DIFFERENTIAL INCORPORATION OF GLUCOSE AND ACETATE INTO BRAIN LIPIDS IN VITRO 总被引:2,自引:2,他引:0
—The oxidation to CO2 and the incorporation of [U-14C]glucose and [U-14C]acetate into lipids by cortex slices from rat brain during the postnatal period were investigated. The oxidation of [U-14C]glucose was low in 2-day-old rat brain, and increased by about two-fold during the 2nd and 3rd postnatal weeks. The oxidation of [U-14C]acetate was increased markedly in the second postnatal week, but decreased to rates observed in 2-day-old rat brain at the time of weaning. Both labeled substrates were readily incorporated into non-saponifiable lipids and fatty acids by brain slices from 2-day-old rat. Their rates of incorporation and the days on which maximum rates occurred were different, however, maximum incorporation of [U-14C]glucose and [U-14]acetate into lipid fractions being observed on about the 7th and 12th postanatal days, respectively. The metabolic compartmentation in the utilization of these substrates for lipogenesis is suggested. The activities of glucose-6-phosphate dehydrogenase, cytosolic NADP-malate dehydrogenase, cytosolic NADP-isocitrate dehydrogenase, ATP-citrate lyase and acetyl CoA carboxylase were measured in rat brain during the postnatal period. All enzymes followed somewhat different courses of development; the activity of acetyl CoA carboxylase was, however, the lowest among other key enzymes in the biosynthetic pathway, and its developmental pattern paralleled closely the fatty acid synthesis from [U-14C]glucose. It is suggested that acetyl CoA carboxylase is a rate-limiting step in the synthesis de novo of fatty acids in developing rat brain. 相似文献
20.
《Insect Biochemistry》1990,20(6):645-652
Post-emergence levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and ketocatechol were determined in cuticle from adult Tenebrio molitor. Possible pathways for biosynthesis of DOPAC were studied by comparing the incorporation of injected [U-14C]tyrosine, [7-14C]dopamine, [7-14C]DOPA, [7-14C]tyramine, [U-14C]p-hydroxyphenylpyruvic acid (p-HPPA) and [ring-3H]p-hydroxyphenylacetic acid (p-HPAA) into cuticular DOPAC during its period of maximal increase 1–3 days after adult emergence. Increased incorporation of [U-14C]tyrosine between days 0 and 3 suggests rapid de novo biosynthesis of DOPAC from this primary precursor. Of the putative intermediates tested, only p-HPPA had a pattern of incorporation similar to that seen with tyrosine. Since p-HPAA was poorly incorporated into both cuticle and DOPAC, a tentative pathway tyrosine → p-HPPA → 3,4-dihydroxyphenylpyruvic acid → DOPAC is proposed. 相似文献