On the Cyprideis species flock (Crustacea, Ostracoda) in Lake Tanganyika, with the description of four new species

Four new species, belonging to three genera, of ostracods from Lake Tanganyika are described: Mesocyprideis nitida sp.nov., Romecytheridea belone sp.nov., R. plegma sp. nov. and Cyprideis loricata sp. nov. The Cyprideis species flock of Lake Tanganyika now consists of 17 formally described species (16 endemic) in 6 genera (4 endemic). The geographical and ecological distribution of the four species is briefly discussed and some supplementary remarks on the radiation of the Cyprideis species flock in Lake Tanganyika are offered.     

Magnesium and strontium in non-marine ostracod shells as indicators of palaeosalinity and palaeotemperature

Measurements of the Ca, Sr, and Mg contents of individual calcitic shells of non-marine ostracods and their host waters, both in lakes and controlled aquaria, permit the calculation of the distribution coefficients of Sr/Ca and Mg/Ca partitioning in ostracod shells. We report new K_D[Sr] for seven genera of non-marine ostracods and K_D[Mg] for Cyprideis at 25°C.Strontium partitioning is virtually temperature-independent, and is related to the Sr/Ca of the host water, and in Ca²⁺-saturated waters, to the salinity of the water. Magnesium partitioning is dependent on both temperature and Mg/Ca of the host water.For simple closed-basin lakes (crater lakes are ideal), the Sr content of ostracods is a sensitive indicator of salinity and thus evaporation/precipitation changes, which in turn, indicate variations in continental climate. A 10000-year continuous palaeosalinity record established by Sr and Mg contents of fossil ostracods for Lake Keilambete, southeastern Australia, is in close agreement with an independent palaeosalinity estimate based on sediment textures.We suggest rules that allow Sr and Mg analyses of suites of individual fossil ostracod shells from lacustrine sediments to be interpreted in terms of palaeosalinity and palaeotemperature variations.     

Ostracodes et incursions marines dans l’Holocène terminal du lac Retba (Cap Vert, Sénégal)

The study of marine incursions based on ostracods is carried out for the first time in the late Holocene, with core sediments of the Lac Retba edge. Twenty-four species are identified along the core; most of them are encountered in the Gulf of Guinea shelf and its adjacent lagoons. For the first time Buntonia olokundudui Reyment and Van Valen, Hermanites foveolata Omatsola and Soudanella africana Omatsola are recorded in Senegal. Four ostracods assemblages are evidenced along the core, corresponding to different stages of the lake evolution. Cyprideis nigeriensis Omatsola, Neomonoceratina iddoensis Omatsola and Aglaiocypris gambiensis Witte dominate the first assemblage; they indicate a lagoon slightly open to the sea and bordered with mangrove swamps under relatively humid climate conditions. The second association yields the most diversified ostracod fauna with C. nigeriensis, N. iddoensis and high proportion of reworked “marine species” which evidence a marine little gulf bordered by mangrove under a humid climate. The third, monospecific association (C. nigeriensis) is typical of a closed and hypersaline lagoon under dry climate conditions. An oligospecific association with C. nigeriensis, Pseudoconcha omatsolai Witte and very scarce reworked “marine species” occurs at the top of the core and characterises an open and hypersaline lagoon under arid climate conditions. Ostracods evidence two marine incursions. The first and the stronger one is dated here for the first time between 1420 and 1250 years B.P. The more recent is the weaker and is not yet dated. Evidence of the beginning of the Retba Lake closure towards 680 years B.P. appears at the top of the core, as indicated by high reduction of ostracods abundance, predominance of reticulate Cyprideis and disappearance of the “marine species”, while salts precipitated at the bottom of the lake.     

Distribution of Recent ostracod species in the Lake Qinghai area in northwestern China and its ecological significance

In order to interpret the climate-change data using the ecological characteristics of the ostracods from the drill cores in Lake Qinghai, the distribution of Recent ostracods was studied in Lake Qinghai area. A total of 34 species belonging to the Ostracoda class of Crustacea were collected from different bodies of water in the Lake Qinghai area, and the ecological information for Recent ostracod species was studied. Among these 34 species, Cypris pubera, Eucypris dulcifons, Ilyocypris sp. 1, Ilyocypris sp. 2, Fabaeformiscandona caudata, Fabaeformiscandona hyalina, Herpetocypris reptans, Prionocypris gansenensis, Potamocypris villosa, Potamocypris smaragdina, Paralimnocythere compressa and Subulacypris sp. were first reported by us in the Lake Qinghai area. Some of the species identified exhibited cosmopolitan distributions, at least in the Holarctic region, but P. gansenensis and Ilyocypris echinata appeared to be restricted to the cold regions in northwestern China.The ecological significance of the primary ostracod species in the Lake Qinghai area was described according to the observations made during our time in the field and according to data from a number of reports. Our results indicate that the species diversity and abundance of ostracods may be related to water salinity in the Lake Qinghai area. The ecological information for ostracods can be used to distinguish different water environments and types based on the characteristics of one species or of an assemblage of several species.     

Oxygen-related facies in Lake Pannon deposits (Upper Miocene) at Budapest-Köbánya

Oxygen availability is considered to have been a major factor in shaping the sedimentary facies and biofacies of a Late Miocene (8–8.5 Ma old) Lake Pannon sequence studied in the Kozma-street outcrop in Budapest-Köbánya. The sequence contains blue clays deposited in low-oxygen conditions between the storm and the fair-weather wave bases and thin intercalations of laminated fine sand interpreted as tempestites. The storms caused temporary oxygenation of the bottom and thus promoted bioturbation, as indicated by the presence of trace fossils belonging to the Arenicolites ichnofacies or a lacustrine equivalent of the marine Skolithos ichnofacies. Fully bioturbated sand layers with the infaunal Dreissenomya and other littoral molluscs indicate longer periods of bottom ventilation. The blue clays immediately above these sand layers also contain abundant and diverse mollusc fauna. Oxygen deficiency on the “shelf” of Lake Pannon was a local phenomenon probably caused by a high organic loading and/or a salinity-induced stratification.     

Meiofauna,trace metals,TOC, sedimentology,and oxygen availability in the Late Miocene sublittoral deposits of Lake Pannon

The Late Miocene fine-grained deposits have been investigated in the Vienna Basin to attest oxygen availability for infauna and epifauna in the sublittoral of the long-lived Lake Pannon. A clay and fine silt of the deeper lacustrine facies of a bay passed vertically into a silty clay, silt, and fine sand rhythmic deposition reflecting successive progradation of brackish prodelta to distal delta front colonized by a dense benthic population. A fully oxygenated environment is supposed in the Congeria subglobosa Beds and prodelta accentuated by ichnofauna, ostracods, and abundant in taxa and specimens, low total organic content, and trace elements concentration. A limited anoxic event caused by temporarily worsened circulation has been detected in non-calcareous greyish-blue homogenous clay with a noticeable high concentration of Ni, Co, and Pb coupled with absence of fossils, bioturbation, and low total organic carbon content.     

Molecular analyses of ostracod flocks from Lake Baikal and Lake Tanganyika

Ancient lakes are excellent laboratories for evolutionary research, where species can be studied in the cradle where they originated. In this article, we investigate two endemic ostracod species flocks from the two oldest lakes in the world, Lake Baikal (LB) (ca. 28 myr) and Lake Tanganyika (LT) (ca. 12 myr), with DNA sequence data. Nuclear ITS1 failed to resolve the phylogeny of both flocks. Whilst most phylogenetic relationships of the Tanganyika flock are resolved with mitochondrial COI, the Baikalian tree contains multifurications of up to seven different clades. The Tanganyikan Cyprideis flock shows higher genetic variability, which matches its higher morphological variability. A significant deviation from a constant divergence rate through time indicates that the Cytherissa flock most likely experienced explosive speciation events during its earlier history. Comparative analyses of substitution rates furthermore revealed that they are not clock-wise for COI. Ancestral Cytherissa probably radiated in LB 5–8 myr ago, around the time when the cold, oxygenated abyss was formed in LB. The Tanganyikan Cyprideis flock is almost twice as old as the Baikalian Cytherissa flock, and possibly older than LT itself, ca. 15 myr. The Cyprideis flock has survived drastic lake level changes and resulting salinity crises during its entire history.     

Presence du Messinien dans la vallee du Rhone

According to the paleontological (molluscs and ostracods) and stratigraphical data, the Congeria rhodanicacclays are of Upper Messinian age. This oligohaline facies points out the local transgression of mediterranean origin in the Rhone basin, following a Lower Messinian erosive episode with coarse subaerial partial valley infill.     

Holocene marine intrusions in Retba and Mbawane lakes (Senegal) evidenced by ostracod faunas

The study of a sea level fluctuation based on ostracods is carried out for the first time using core sediments of Mbawane and Retba Lakes, north coast of Senegal. A total of 26 species have been identified and most of them are encountered in the Gulf of Guinea shelf and its adjacent lagoons. The Retba Lake core, which is closer to the seashore, has yielded a richer and more diversified ostracod fauna composed of four assemblages. The F1 assemblage is composed of Cyprideis nigeriensis, Neomonoceratina iddoensis, and Aglaiocypris gambiensis, which characterises a slightly open lagoon developing under a humid climate with its edges covered with mangrove swamps. Assemblage F2, composed mainly of C. nigeriensis, N. iddoensis and “marine species”, which is richer and more diversified, points to a marine little gulf bordered by mangrove swamps. The F3 monospecific assemblage composed of C. nigeriensis indicates a drying confined lagoon under an arid climate. The F4 oligospecific association at the top, composed of C. nigeriensis and Pseudoconcha omatsolai, is typical of a slightly open saline lagoon under arid climate. Core sediments from Mbawane Lake have yielded scarce and badly preserved microfaunas which are distributed into three discontinuous levels that have been correlated to those of the Retba Lake core assemblages. The F1 assemblage composed of Leptocythere culpata and Xestoleberis sp. 2 corresponds to a slightly open lagoon covered at its bottom by marine macrophytes and algae. The F2 assemblage composed of N.iddoensis, Hemicytherura sp. A, Aglaiocypris gambiensis and Ruggieria tricostata characterises a more open lagoon bordered by mangrove swamps under a humid climate. The F3 assemblage is absent in the Mbawane Lake. The F4 monospecific association composed of C. nigeriensis characterises a drying lagoon under an arid climate. The overall marine influences were stronger in the Retba Lake while continental effects were dominant in Mbawane Lake during the same period. The two marine intrusions mentioned above may correspond to those of the Dakarian (3000 years B.P.) and the Saint-Louisian eras (2000–680 years B.P.) that existed during the Upper Holocene on the Senegalese coast.     

Molecular identification of species and ploidy of Carassius fishes in Lake Biwa,using mtDNA and microsatellite multiplex PCRs

We established an efficient and reliable method to identify the species and ploidy of Carassius fishes in Lake Biwa (C. cuvieri, C. buergeri, and the latter’s polyploid) using a combination of multiplex mitochondrial DNA haplotype-specific PCR and ploidy determination based on nuclear DNA microsatellite PCR. The high accuracy of ploidy determination was confirmed by flow cytometry. This method can also identify triploid clonal lines and provide information about their genetic diversity and structure. The method is applicable to ecological and evolutionary studies of Carassius fishes in Lake Biwa throughout their entire life history.     

The Neogene Ostracod Genus <Emphasis Type="Italic">Advenocypris</Emphasis> from the Intermontane Depressions of the Southeastern Altai

This paper presents new data on ostracods of the genus Advenocypris. The distribution of these ostracods in the Neogene deposits of the intermontane depressions of Central Asia is analyzed. Species of this genus were selective regarding their habitats. This habitat specificity can be used to reconstruct the paleogeography of the depositional environments, origin and connections of water bodies in Central Asia. A new species of the genus Advenocypris is described. The previously known species of this genus are illustrated.     

An overlooked hybrid between the two diploid <Emphasis Type="Italic">Chenopodium</Emphasis> species in Central Europe determined by microsatellite and morphological analysis

The presence and extent of hybridization within the Chenopodium album aggregate (Amaranthaceae) is still unclear. Although many hybrid combinations have been described, their existence in the field has never been systematically studied and verified. The main aim of this study was to ascertain the extent of interspecific hybridization between the diploid species C. ficifolium and C. suecicum using highly variable nuclear microsatellite markers. Due to the absence of such kind of molecular markers for the whole C. album group, we divided the analysis into two steps: (1) Eleven microsatellite loci designed for the closely related species C. quinoa were cross-amplified in five Eurasian species of the C. album diploid–polyploid complex, i.e. C. album s.s. (6x), C. striatiforme (4x), C. strictum (4x), C. ficifolium (2x) and C. suecicum (2x); (2) For the detection of interspecific hybridization between C. ficifolium and C. suecicum, we sampled 480 individuals from five localities in Central Europe. We also investigated morphological differences between the parental taxa and their hybrid and devised a key for their determination. Analysis of variation in microsatellite loci using Bayesian methods, PCoA and Neighbour-joining tree identified 32 F1 hybrids. These F1 hybrids, described here as C. paradoxum Mandák, formed a cluster between well-differentiated parental species, combining the morphological characters of both their parents. Moreover, genetic analyses also recognized several F2 or backcross hybrids, whose delimitation, mainly from C. suecicum and F1 hybrids, based on morphological characters, is problematic.     

Introgressive Hybridization and the Evolution of Lake-Adapted Catostomid Fishes

Hybridization has been identified as a significant factor in the evolution of plants as groups of interbreeding species retain their phenotypic integrity despite gene exchange among forms. Recent studies have identified similar interactions in animals; however, the role of hybridization in the evolution of animals has been contested. Here we examine patterns of gene flow among four species of catostomid fishes from the Klamath and Rogue rivers using molecular and morphological traits. Catostomus rimiculus from the Rogue and Klamath basins represent a monophyletic group for nuclear and morphological traits; however, the Klamath form shares mtDNA lineages with other Klamath Basin species (C. snyderi, Chasmistes brevirostris, Deltistes luxatus). Within other Klamath Basin taxa, D. luxatus was largely fixed for alternate nuclear alleles relative to C. rimiculus, while Ch. brevirostris and C. snyderi exhibited a mixture of these alleles. Deltistes luxatus was the only Klamath Basin species that exhibited consistent covariation of nuclear and mitochondrial traits and was the primary source of mismatched mtDNA in Ch. brevirostris and C. snyderi, suggesting asymmetrical introgression into the latter species. In Upper Klamath Lake, D. luxatus spawning was more likely to overlap spatially and temporally with C. snyderi and Ch. brevirostris than either of those two with each other. The latter two species could not be distinguished with any molecular markers but were morphologically diagnosable in Upper Klamath Lake, where they were largely spatially and temporally segregated during spawning. We examine parallel evolution and syngameon hypotheses and conclude that observed patterns are most easily explained by introgressive hybridization among Klamath Basin catostomids.     

Conspecific tolerance and heterospecific competition as mechanisms for overcoming resistance to invasion by an intertidal crab

The success of the invasive Asian shore crab, Hemigrapsus sanguineus, stems partly from its ability to exclude established crab species from preferred rocky and cobble intertidal habitat. Here, we assessed preference and competition for habitat types (cobble vs. sand) for H. sanguineus and two competitor species; the previous invasive green crab, Carcinus maenas, and the native rock crab, Cancer irroratus, in New England. In simple laboratory experiments, we paired similarly sized heterospecifics and conspecifics from each species, and also grouped combinations of C. maenas and H. sanguineus in a series of four-individual sets in order to dissect the outcome of intra- and inter-specific competition at different densities. Individually, all three species preferred cobble substrate. With paired conspecifics, H. sanguineus individuals would cohabitate in cobble, whereas C. maenas and C. irroratus individuals each excluded conspecifics from cobble. In heterospecific pairs, H. sanguineus excluded both C. maenas and C. irroratus from cobble. C. maenas and C. irroratus, were equally likely to exclude the other species, but rarely excluded H. sanguineus. In larger assemblages, H. sanguineus preferentially grouped under cobble, whereas C. maenas were more evenly distributed among habitat types. These observations demonstrate that conspecific tolerance and heterospecific competition can be effective, complementary mechanisms for overcoming invasion resistance. Such mechanisms help explain the well-studied success of H. sanguineus following its introduction into New England coastal habitats, and the resulting exclusion of preexisting crab species.     

Invasive South American floating plants are a successful substrate for native Central African pleuston

Eichhornia crassipes, commonly known as water hyacinth, is a free-floating perennial aquatic plant native to South America, which has been widely introduced on different continents, including Africa. E. crassipes is abundant in both the Congo (Africa) and Amazon (South America) River catchments. We performed a comparative analysis of the ostracod communities (Crustacea, Ostracoda) in the E. crassipes pleuston in the Amazon (South America) and Congo (Africa) River catchments. We also compared the ostracod communities from the invasive E. crassipes with those associated with stands of the native African macrophyte Vossia cuspidata. We recorded 25 species of ostracods associated with E. crassipes in the Amazon and 40 in the Congo River catchments, distributed over 31 ostracod species in E. crassipes and 27 in V. cuspidata. No South American invasive ostracod species were found in the Congolese pleuston. Diversity and richness of Congolese ostracod communities was higher in the invasive (Eichhornia) than in a native plant (Vossia). The highest diversity and abundance of ostracod communities were recorded in the Congo River. The result of principal coordinates analysis, used to evaluate the (dis)similarity between different catchments, showed significant differences in species composition of the communities. However, a dispersion homogeneity test (PERMDISP) showed no significant differences in the variability of the composition of species of ostracods (beta diversity) within Congo and Amazon River catchments. It appears that local ostracod faunas have adapted to exploit the opportunities presented by the floating invasive Eichhornia, which did not act as “Noah’s Ark” by introducing South American ostracods in the Congo River.     

A Re-Evaluation of the Chasmosaurine Ceratopsid Genus Chasmosaurus (Dinosauria: Ornithischia) from the Upper Cretaceous (Campanian) Dinosaur Park Formation of Western Canada

Background

The chasmosaurine ceratopsid Chasmosaurus is known from the Upper Cretaceous (Campanian) Dinosaur Park Formation of southern Alberta and Saskatchewan. Two valid species, Chasmosaurus belli and C. russelli, have been diagnosed by differences in cranial ornamentation. Their validity has been supported, in part, by the reported stratigraphic segregation of chasmosaurines in the Dinosaur Park Formation, with C. belli and C. russelli occurring in discrete, successive zones within the formation.

Results/Conclusions

An analysis of every potentially taxonomically informative chasmosaurine specimen from the Dinosaur Park Formation indicates that C. belli and C. russelli have indistinguishable ontogenetic histories and overlapping stratigraphic intervals. Neither taxon exhibits autapomorphies, nor a unique set of apomorphies, but they can be separated and diagnosed by a single phylogenetically informative character—the embayment angle formed by the posterior parietal bars relative to the parietal midline. Although relatively deeply embayed specimens (C. russelli) generally have relatively longer postorbital horncores than specimens with more shallow embayments (C. belli), neither this horncore character nor epiparietal morphology can be used to consistently distinguish every specimen of C. belli from C. russelli.

Status of Kosmoceratops in the Dinosaur Park Formation

Kosmoceratops is purportedly represented in the Dinosaur Park Formation by a specimen previously referred to Chasmosaurus. The reassignment of this specimen to Kosmoceratops is unsupported here, as it is based on features that are either influenced by taphonomy or within the realm of individual variation for Chasmosaurus. Therefore, we conclude that Kosmoceratops is not present in the Dinosaur Park Formation, but is instead restricted to southern Laramidia, as originally posited.     

Morphology,Molecules, and Monogenean Parasites: An Example of an Integrative Approach to Cichlid Biodiversity

The unparalleled biodiversity of Lake Tanganyika (Africa) has fascinated biologists for over a century; its unique cichlid communities are a preferred model for evolutionary research. Although species delineation is, in most cases, relatively straightforward, higher-order classifications were shown not to agree with monophyletic groups. Here, traditional morphological methods meet their limitations. A typical example are the tropheine cichlids currently belonging to Simochromis and Pseudosimochromis. The affiliations of these widespread and abundant cichlids are poorly understood. Molecular work suggested that genus and species boundaries should be revised. Moreover, previous morphological results indicated that intraspecific variation should be considered to delineate species in Lake Tanganyika cichlids. We review the genera Simochromis and Pseudosimochromis using an integrative approach. Besides a morphometric study and a barcoding approach, monogenean Cichlidogyrus (Platyhelminthes: Ancyrocephalidae) gill parasites, often highly species-specific, are used as complementary markers. Six new species are described. Cichlidogyrus raeymaekersi sp. nov., C. muterezii sp. nov. and C. banyankimbonai sp. nov. infect S. diagramma. Cichlidogyrus georgesmertensi sp. nov. was found on S. babaulti and S. pleurospilus, C. franswittei sp. nov. on both S. marginatus and P. curvifrons and C. frankwillemsi sp. nov. only on P. curvifrons. As relatedness between Cichlidogyrus species usually reflects relatedness between hosts, we considered Simochromis monotypic because the three Cichlidogyrus species found on S. diagramma belonged to a different morphotype than those found on the other Simochromis. The transfer of S. babaulti, S. marginatus, S. pleurospilus and S. margaretae to Pseudosimochromis was justified by the similarity of their Cichlidogyrus fauna and the intermediate morphology of S. margaretae. Finally parasite data also supported the synonymy between S. pleurospilus and S. babaulti, a species that contains a large amount of geographical morphological variation.