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1.
Peter Rautenberg Regina Schädler Erwin Reinwald Hans-Jörg Risse 《Molecular and cellular biochemistry》1982,47(3):151-159
Summary A protease has been purified from Trypanosoma congolense bloodstream forms by osmotic disruption, freeze-thawing of the cells, followed by chromatography using Thiopropyl-Sepharose and gel filtration.The enzyme is a thiolprotease. A combination of SDS-polyacrylamide gel electrophoresis and contact print zymograms using casein as substrate showed a single proteolytic band with a molecular weight of 31 000. The isoelectric point of the enzyme as ascertained by isoelectric focusing extended from pH 4.4 to 5.5 with a maximum at pH 5.0. The protease cleaved various heat denatured substrates such as casein, hemoglobin, albumin and ovalbumin. The highest enzyme activity was observed at pH 5.5 and pH 6.0 using casein and hemoglobin as substrates respectively. The max. temperature was found to be 50 °C. The enzyme is inactivated by mercurial compounds, iodoacetamide, iodoacetate, chloromethylketones and leupeptin and is activated by dithioerythritol. 相似文献
2.
VARIATIONS IN AROMATIC AMINO ACID DECARBOXYLASE ACTIVITY TOWARDS DOPA AND 5-HYDROXYTRYPTOPHAN CAUSED BY pH CHANGES AND DENATURATION 总被引:2,自引:1,他引:1
—DOPA and 5-hydroxytryptophan (5-HTP) are generally supposed to be decarboxylated in mammalian tissues by a single enzyme, the two activities being present in constant ratio through a variety of purification procedures. It has now been shown that the ratio of activity of the liver enzyme towards the two substrates can be altered by mild treatments, such as might be used in solubilization of brain preparations. DOPA decarboxylase activity was preferentially inactivated by sodium dodecyl sulphate treatment, and 5-HTP decarboxylation by urea. Previous reports that the two substrates show different pH optima but are mutually competitive, have been confirmed. The Km of the enzyme towards 5-HTP was lowest at pH 7.8 (the optimum pH for decarboxylation of this amino acid), but the variation with pH of the Km towards DOPA was unrelated to the pH optimum for decarboxylation. There appeared to be no relation between the probable ionization state of the substrates and the pH dependence of the enzyme. Studies on the binding characteristics of the enzyme for the two products, dopamine and serotonin, did not show any specific saturable binding. It is proposed that the enzyme has a complex active site, with separate affinity sites for the two substrates, adjacent to a single catalytic site. 相似文献
3.
Hans-Peter Schär Daniel Gygax Gerardo M. Ramos Tombo Oreste Ghisalba 《Applied microbiology and biotechnology》1988,27(5-6):451-456
Summary An acetyl specific carboxylesterase has been purified from Nocardia mediterranei. The purified enzyme is homogeneous as shown by SDS polyacrylamide gel electrophoresis. The esterase has a molecular weight of 68,000 and is composed of two identical subunits. The enzyme exhibits optimal activity at pH 7.5 and at 35°C and is stable below 40°C. The enzyme activity is inhibited by several sulfhydryl reagents. The esterase hydrolyzes preferentially acetyl esters. Propionyl esters are cleaved very slowly whereas butyryl esters are no substrates at all. In addition, the esterase shows a pronounced regiospecificity. On the other hand the enantiospecificity is rather low as demonstrated by the hydrolysis of prochiral and racemic substrates. 相似文献
4.
Arai T Kosugi A Chan H Koukiekolo R Yukawa H Inui M Doi RH 《Applied microbiology and biotechnology》2006,71(5):654-660
The cellulosomal family 9 cellulase genes engH, engK, engL, engM, and engY of Clostridium cellulovorans have been cloned and sequenced. We compared the enzyme activity of family 9 cellulosomal cellulases from C. cellulovorans and their derivatives. EngH has the highest activity toward soluble cellulose derivatives such as carboxymethylcellulose (CMC) as well as insoluble cellulose such as acid-swollen cellulose (ASC). EngK has high activity toward insoluble cellulose such as ASC and Avicel. The results of thin-layer chromatography showed that the cleavage products of family 9 cellulases were varied. These results indicated that family 9 endoglucanases possess different modes of attacking substrates and produce varied products. To investigate the functions of the carbohydrate-binding module (CBM) and the catalytic module, truncated derivatives of EngK, EngH, and EngY were constructed and characterized. EngHΔCBM and EngYΔCBM devoid of the CBM lost activity toward all substrates including CMC. EngKΔCBM and EngMΔCBM did not lose activity toward CMC but lost activity toward Avicel. These observations suggest that the CBM is extremely important not only because it mediates the binding of the enzyme to the substrates but also because it participates in the catalytic function of the enzyme or contributes to maintaining the correct tertiary structure of the family 9 catalytic module for expressing enzyme activity. 相似文献
5.
The NADP+-specific glutamate dehydrogenase fromEscherichia coli has been purified to electrophoretic homogeneity. The enzyme was purified 40-fold and has a specific activity of 23. Glutamate dehydrogenase fromE. coli is a hexameric enzyme with a native molecular weight of 275 KDa composed of monomers each with a molecular weight of 44.5 KDa. In nondenaturing isoelectric focusing gels, the purified enzyme is resolved into six catalytically active species, each with a molecular weight of 275 KDa and with isoelectric points ranging between pH 5.3 and 5.7. The Km values for substrates and coenzymes have been determined, and the effect of several divalent ions on catalytic activity has been investigated. 相似文献
6.
Yasuji Minoda Ryuichiro Kurane Koichi Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(11):2511-2516
This paper presents a study on the enzyme reduction of the disulfide bond and the following results have been found.In enzyme preparation, antioxidants showed a stability effect and EDTA appeared to have both enzyme stabilization and solubilization. On the distribution of the enzyme activity in subcellular fractions, the water soluble fraction appeared to contain the major released enzyme activity. The enzyme was inhibited with several metals. Hg2+ and transition metals were the most toxic. The substrate specificity of this enzyme was wide for the low molecular substrates, but the protein disulfide reducing activity was not detected in this preparation. It was assumed that the thiol-disulfide transhydrogenase was coupled with glutathione reductase and the disulfide substrates were reduced by the system involving the two enzymes. A new method for the direct recording of an enzyme-catalyzed thiol-disulfide interchange using diphenyl disulfide and p,p-dinitro diphenyl disulfide was devised. 相似文献
7.
Vinita Yadav Saroj Yadav Sarita Yadav K. D. S. Yadav 《Applied Biochemistry and Microbiology》2012,48(3):295-301
An a-L-rhamnosidase secreting fungal strain has been isolated and identified as Aspergillus clavato-nanicus MTCC-9611. The enzyme was purified to homogeneity from the culture filtrate of the fungus using concentration by ultrafiltration
membrane and ion-exchange chromatography on CM-cellulose. The native PAGE analysis confirmed the homogeneity of the purified
enzyme. The SDS-PAGE analysis of the purified enzyme revealed a single protein band corresponding to the molecular weight
82 kDa. The α-L-rhamnosidase activity of Aspergillus clavato-nanicus MTCC-9611 had optimum at pH 10.0 and 50°C. The K
m values of the enzyme were 0.65 mM and 0.95 mM using p-nitrophenyl α-L-rhamnopyranoside and naringin as a substrates respectively.
The enzyme transforms naringin to prunin at pH 10.0 and further hydrolysis of prunin to naringenin does not occur under these
reaction conditions that makes α-L-rhamnosidase activity of Aspergillus clavato-nanicus MTCC-9611 promising enzyme to get prunin for pharmaceutical purposes. 相似文献
8.
Joanne Blondin Ronald H. Goldstein Carl Franzblau 《In vitro cellular & developmental biology. Plant》1982,18(1):15-23
Summary Gelatin-specific protease activity from hamster lung fibroblasts and their culture media is described. The fibroblasts were
derived from hamster lung explant cultures. The gelatin-specific protease activity is latent and seen only after dialysis
of either cells or media. The enzyme activity shares many properties of previously reported gelatinases. The activity is inhibited
by EDTA, cysteine, and dithioerythritol, whereas it is not inhibited byp-chloromecuribenzoate,N-ethyl maleimide, or phenylmethylsulfonyl fluoride. Of all substrates tested, activity was observed only against gelatin and
not against other substrates tested. It was inactive toward collagen, elastin, and methemoglobin. This enzyme may have a role
in the digestion of collagen that has been previously cleaved by mammalian collagenase.
This research was supported by Program Project Grant HL-19717 from the National Heart, Lung, and Blood Institute, Grant AG
000-38-02 from the National Institute of Aging, and National Institute of Health Grant 5T32HL07035. 相似文献
9.
Summary
Mucor miehei lipase has been adsorbed on Celite and covalently bound to nylon. The obtained derivatives have been studied regarding their ability for synthetize several flavouring esters in biphasic aqueous/organic media. The influence of the immobilization procedure on the synthetic activity of the derivatives was considered. Solvent hydrophobicity and water content in the biphasic system influenced both enzyme stability and equilibrium displacements. In this way, solvents with log P>3.5 and less than 1% water were optimal. It was important to consider pH effects on enzyme microenvironment when using acidic substrates. Optimum temperature and reuse of the catalyst were also checked. 相似文献
10.
Purification and biochemical characterization of a fibrinolytic enzyme from Bacillus subtilis BK-17 总被引:1,自引:0,他引:1
Jeong Yong Kee Park Jeong Uck Baek Hyun Park Sung Hoon Kong In Soo Kim Dong Wan Joo Woo Hong 《World journal of microbiology & biotechnology》2001,17(1):89-92
A fibrinolytic enzyme from Bacillus subtilis BK-17 has been purified to homogeneity by gel-filtration and ion-exchange chromatography. Compared to the crude enzyme extract, the specific activity of the enzyme increased 929-fold with a recovery of 29%. The subunit molecular mass of the purified enzyme was estimated to be 31 kDa by SDS–PAGE. The N-terminal amino acid sequence of the purified fibrinolytic enzyme was: A-Q-S-V-P-Y-G-V-S-Q-I-K-A-P-A-A-H-N. The sequence was highly homologous to the fibrinolytic enzymes nattokinase, subtilisin J and subtilisin E from Bacillus spp. However, there was a substitution of three amino acid residues in the N-terminal sequence. The amidolytic activity of the purified enzyme for several substrates was assessed. In comparison with nattokinase and CK (fibrinolytic enzyme from a Bacillus spp.), which showed strong fibrinolytic activity, the amidolytic activity of the enzyme for the synthetic substrate, kallikrein (H-D-Val-Leu-Arg-pNA, S-2266) increased 2.4- and 11.8-fold, respectively. 相似文献
11.
In tropical legumes like French bean (Phaseolus vulgaris) or soybean (Glycine max), most of the atmospheric nitrogen fixed in nodules is used for synthesis of the ureides allantoin and allantoic acid, the
major long distance transport forms of organic nitrogen in these species. The purpose of this investigation was to characterise
the allantoate degradation step in Phaseolus vulgaris. The degradation of allantoin, allantoate and ureidoglycolate was determined “in vivo” using small pieces of chopped seedlings.
With allantoate and ureidoglycolate as substrates, the determination of the reaction products required the addition of phenylhydrazine
to the assay mixture. The protein associated with the allantoate degradation has been partially purified 22-fold by ultracentrifugation
and batch separation with DEAE-Sephacel. This enzyme was specific for allantoate and could not use ureidoglycolate as substrate.
The activity was completely dependent on phenylhydrazine, which acts as an activator at low concentrations and decreases the
affinity of the enzyme for the substrate at higher concentrations. The optimal pH for the activity of the purified protein
was 7.0 and the optimal temperature was 37°C. The activity was completely inhibited by EDTA and only manganese partially restored
the activity. The level of activity was lower in extracts obtained from leaves and fruits of French bean grown with nitrate
than in plants actively fixing nitrogen and, therefore, relying on ureides as nitrogen supply. This is the first time that
an allantoate-degrading activity has been partially purified and characterised from a plant extract. The allosteric regulation
of the enzyme suggests a critical role in the regulation of ureide degradation. 相似文献
12.
A. Padiglia Rosaria Medda Jens Z. Pedersen Alessandro Finazzi Agrò Anita Lorrai Barbara Murgia Giovanni Floris 《Journal of biological inorganic chemistry》1999,4(5):608-613
The reaction with substrates and carbonyl reagents of native lentil Cu-amine oxidase and its modified forms, i.e. Cu-fully-depleted,
Cu-half-reconstituted, Cu-fully-reconstituted, Co-substituted, Ni-substituted and Zn-substituted, has been studied. Upon removal
of only one of the two Cu ions, the enzyme loses 50% of its enzymatic activity. Using several substrates, Co-substituted lentil
amine oxidase is shown to be active but the k
c value is different from that of native or Cu-fully-reconstituted enzyme, while K
m is similar. On the other hand, the Ni- and Zn-substituted forms are catalytically inactive. Enzymatic activity measurements
and optical spectroscopy show that only in the Co-substituted enzyme is the organic cofactor 6-hydroxydopa quinone reactive
and the enzyme catalytically competent, although less efficient. The Co-substituted amine oxidase does not form the semiquinone
radical as an intermediate of the catalytic reaction. While devoid or reduced of catalytic activity, all the enzyme preparations
are still able to oxidise two moles of substrate and to release two moles of aldehyde per mole of dimeric enzyme. The results
obtained show that although Co-substituted amine oxidase is catalytically competent, copper is essential for the catalytic
mechanism.
Received: 5 March 1999 / Accepted: 22 July 1999 相似文献
13.
Matthew D. Lloyd Kieren D.E. Boardman Andrew Smith Daan M. van den Brink Ronald J.A. Wanders Michael D. Threadgill 《Journal of enzyme inhibition and medicinal chemistry》2013,28(5):584-590
Fatty aldehyde dehydrogenase (FALDH) is an NAD+-dependent oxidoreductase involved in the metabolism of fatty alcohols. Enzyme activity has been implicated in the pathology of diabetes and cancer. Mutations in the human gene inactivate the enzyme and cause accumulation of fatty alcohols in Sjögren-Larsson syndrome, a neurological disorder resulting in physical and mental handicaps. Microsomal FALDH was expressed in E. coli and purified. Using an in vitro activity assay an optimum pH of ~9.5 and temperature of ~35°C were determined. Medium- and long-chain fatty aldehydes were converted to the corresponding acids and kinetic parameters determined. The enzyme showed high activity with heptanal, tetradecanal, hexadecanal and octadecanal with lower activities for the other tested substrates. The enzyme was also able to convert some fatty alcohol substrates to their corresponding aldehydes and acids, at 25–30% the rate of aldehyde oxidation. A structural model of FALDH has been constructed, and catalytically important residues have been proposed to be involved in alcohol and aldehyde oxidation: Gln-120, Glu-207, Cys-241, Phe-333, Tyr-410 and His-411. These results place FALDH in a central role in the fatty alcohol/acid interconversion cycle, and provide a direct link between enzyme inactivation and disease pathology caused by accumulation of alcohols. 相似文献
14.
A. J. Van Winkelhoff T. J. M. Van Steenbergen N. Kippuw J. De Graaff 《Antonie van Leeuwenhoek》1986,52(2):163-171
The purpose of this study was to investigate the production of various enzymes by oral and non-oral black-pigmented Bacteroides species using chromogenic substrates. The 19 substrates present in the API ZYM system did not differentiate between B. melaninogenicus, B. denticola, B. loescheii and B. levii. The asaccharolytic black-pigmented Bacteroides species showed each species specific enzyme activity, however, differences were based on one enzyme only as far as B. asaccharolyticus and B. endodontalis are concerned. An extended number of 40 chromogenic substrates were tested in order to find more species specific enzyme. With a set of 20 substrates it appeared to be possible to discriminate between all species tested. The possibility to use enzymes for the identification of black-pigmented Bacteroides is discussed. 相似文献
15.
Biochemical characterization of two haloalkane dehalogenases: DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea
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Vladimir N. Malashkevich Steven C. Almo Emily C. Mundorff 《Protein science : a publication of the Protein Society》2016,25(4):877-886
Two putative haloalkane dehalogenases (HLDs) of the HLD‐I subfamily, DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea, have been identified based on sequence comparisons with functionally characterized HLD enzymes. The two genes were synthesized, functionally expressed in E. coli and shown to have activity toward a panel of haloalkane substrates. DsaA has a moderate activity level and a preference for long (greater than 3 carbons) brominated substrates, but little activity toward chlorinated alkanes. DccA shows high activity with both long brominated and chlorinated alkanes. The structure of DccA was determined by X‐ray crystallography and was refined to 1.5 Å resolution. The enzyme has a large and open binding pocket with two well‐defined access tunnels. A structural alignment of HLD‐I subfamily members suggests a possible basis for substrate specificity is due to access tunnel size. 相似文献
16.
Dunn RV Daniel RM 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2004,359(1448):1309-20; discussion 1320, 1323-8
Although there are varying estimates as to the degree of enzyme hydration required for activity, a threshold value of ca. 0.2 g of water per gram of protein has been widely accepted. The evidence upon which this is based is reviewed here. In particular, results from the use of gas-phase substrates are discussed. Results using solid-phase enzyme-substrate mixtures are not altogether in accord with those obtained using gas-phase substrates. The use of gaseous substrates and products provides an experimental system in which the hydration of the enzyme can be easily controlled, but which is not limited by diffusion. All the results show that increasing hydration enhances activity. The results using gas-phase substrates do not support the existence of a critical hydration value below which enzymatic activity is absent, and suggest that enzyme activity is possible at much lower hydrations than previously thought; they do not support the notion that significant hydration of the surface polar groups is required for activity. However, the marked improvement of activity as hydration is increased suggests that water does play a role, perhaps in optimizing the structure or facilitating the flexibility required for maximal activity. 相似文献
17.
By comparing different activity data of the buffered cellulase solution before and after contact with the substrate the interaction between Penicillium janthinellum cellulase and wheat straw, resp. its components (holocellulose and isolated lignin) has been investigated. The loss of activity due to sorption or denaturation has been found to differ widely between the different activity data and between the various substrates. A remarkable loss of enzyme activity was observed after contact with isolated straw lignin. The differences in activity decrease between the cellulose and the lignin moiety were found to be largent with the cellobiase activity. 相似文献
18.
Maurice J. Bessman 《Protein science : a publication of the Protein Society》2019,28(8):1494-1500
The Nudix hydrolase superfamily is identified by a conserved cassette of 23 amino acids, and it is characterized by its pyrophosphorylytic activity on a wide variety of nucleoside diphosphate derivatives. Of the 13 members of the family in Escherichia coli, only one, Orf180, has not been identified with a substrate, although a host of nucleoside diphosphate compounds has been tested. Several reports have noted a strong similarity in the three‐dimensional structure of the unrelated enzyme, isopentenyl diphosphate isomerase (IDI) to the Nudix structure, and the report that a Nudix enzyme was involved in the synthesis of geraniol, a product of the two substrates of IDI, prompted an investigation of whether the IDI substrates, isopentenyl diphosphate (IPP), and dimethylallyl diphosphate (DAPP) could be substrates of Orf180. This article demonstrates that Orf180 does have a very low activity on IPP, DAPP, and geranyl pyrophosphate (GPP). However, several of the other Nudix enzymes with established nucleoside diphosphate substrates hydrolyze these compounds at substantial rates. In fact, some Nudix hydrolases have higher activities on IPP, DAPP, and GPP than on their signature nucleoside diphosphate derivatives. 相似文献
19.
M. O. Zeltina M. P. Leite A. J. Apine U. A. Bariss J. E. Shvinka 《Folia microbiologica》1991,36(3):263-266
An express method of testing the cellulolytic activity ofTrichoderma viride in media with cellulose-containing substrates by the rate of glucose production has been elaborated. The optimum temperature
selections and duration of sample incubation (1 h, 50°C) have been described, and the temperature coefficient for the scale-down
of the enzyme complex from 50 to 30°C (0.77) has been determined. 相似文献
20.
Expanding the scope of stereoselectivity is of current interest in enzyme catalysis. In this study, using error-prone polymerase
chain reaction (PCR), a thermostable adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) from Thermus caldophilus GK-24 has been altered to improve its catalytic activity toward enatiomeric substrates including [glucose-1-phosphate (G-1-P)
+ uridine triphosphate (UTP)] and [N-acetylglucosamine-1-phosphate (GlcNAc) + UTP] to produce uridine diphosphate (UDP)-glucose and UDP-N-acetylglucosamine, respectively. To elucidate the amino acids responsible for catalytic activity, screening for UDP-glucose
pyrophosphorylase (UGPase) and UDP-N-acetylglucosamine pyrophosphorylase (UNGPase) activities was carried out. Among 656 colonies, two colonies showed UGPase
activities and three colonies for UNGPase activities. DNA sequence analyses and enzyme assays showed that two mutant clones
(H145G) specifically have an UGPase activity, indicating that the changed glycine residue from histidine has the base specificity
for UTP. Also, three double mutants (H145G/A325V) showed a UNGPase, and A325 was associated with sugar binding, conferring
the specificity for the sugar substrates and V325 of the mutant appears to be indirectly involved in the binding of the N-acetylamine group of N-acetylglucosmine-1-phosphate.
The authors Hosung Sohn and Yong-Sam Kim equally contributed to the study. 相似文献