共查询到19条相似文献,搜索用时 55 毫秒
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爱沙木组织培养快速繁殖(简报) 总被引:1,自引:0,他引:1
以爱沙木(Eremophila macdonnellii)种子为外植体,在不加任何生长调节剂的 MS 培养基上,种子可萌发成无菌苗;无菌苗带芽茎段在 MS 6-BA 2.0mg/L NAA 0.5mg/L 增殖培养基上,增殖倍数可达 5.0;在1/2MS NAA 0.5mg/L 1%活性碳的生根培养基上,生根率达 90%。 相似文献
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1植物名称黄葵(Abelmoschus moschatus Medic),别名山油麻、野棉花、山芙蓉、麝香秋葵。2材料类别无菌苗顶芽。3培养条件(1)初级培养基:MS;(2)不定芽诱导培养基:MS 6-BA 0.6 mg·L~(-1)(单位下同) NAA 0.2;(3)增殖与继代培养基:MS 6-BA 1.0 NAA 0.1;(4)生根培养基:1/2MS NAA 0.2。以上培养基均加3%蔗糖和0.5%琼脂,pH 5.8。培养温度为(25±3)℃,光强40~60μmol·m~(-2)·S~(-1),光照时间12~14 h·d~(-1)。 相似文献
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Wennuan Liu C. Ernie Chilcott Richard C. Reich Gary M. Hellmann 《In vitro cellular & developmental biology. Plant》2000,36(3):201-206
Summary The present work provides a system for regeneration of clary sage, (Salvia sclarea L.) via organogenesis using plant tissue culture techniques in a multistage culturing medium containing 2,4-dichlorophenoxyacetic
acid (2,4-D) (9.05–181.00 μM). A higher frequency of organogenic tissue initiation was obtained from immature zygotic embryo cotyledons (IZEC) 2–3 wk
after pollination on the medium supplemented with 9.05 μM 2,4-D. The organogenic tissues were then proliferated on media containing both indole-3-acetic acid (IAA) and 6-benzylaminopurine
(BA). Organogenic lines were established via selection, isolation and continuous subculture of organogenic tissues on a medium
containing 22.19 μM BA and 2.85 μM IAA. Shoots were regenerated from both the proliferated tissues and IZEC, and propagated in the presence of IAA or α naphthaleneacetic
acid (NAA), BA and gibberellic acid (GA3). Although roots were induced from regenerated shoots on the media containing a low concentration of IAA, IBA (0.98 μM) in combination with desiccation of regenerated shoots with a stem ∼10 mm in length promoted more and stronger root formation.
After the root system was well established (20 mm in length), the regenerated plants were transferred to soil in plastic pots
for further growth and production of R1 seeds in the greenhouse. 相似文献
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1 植物名称新疆鼠尾草(Salvia deserta Schang). 2 材料类别种子萌发形成的幼嫩叶片. 3 培养条件基本培养基为MS.(1)丛生芽诱导及增殖培养基:MS 6-BA 2.0 mg·L-1(单位下同) NAA0.25;(2)生根培养基:1/2MS IBA 0.5.培养基(1)的蔗糖浓度为3.0%,(2)的蔗糖浓度为1.5%;琼脂为7.0 g·L-1,pH 6.0. 相似文献
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濒危植物珙桐的组织培养与植株再生 总被引:3,自引:0,他引:3
以珙桐冬芽为材料进行组织培养和植株再生研究,结果表明:珙桐冬芽直接诱导丛生芽的最适培养基为WPM+NAA 0.2 mg·L-1+6-BA 3.0 mg·L-1+AC 2.0 g·L-1;珙桐带芽茎段增殖的适宜培养基为WPM+NAA 0.05 mg·L-1+6-BA 1.0 mg·L-1+GA3 2.0 mg·L-1+AC 2.0 g·L-1;生根最佳培养基为White+IBA3.0 mg·L-1+6-BA 1.0 mg·L-1+AC 2.0 g·L-1,在此条件下,根发育良好,植株健壮;组培苗炼苗后移栽,成活率可达80%。 相似文献
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通过对牛蒡(A rctium lapp a L.)不同外植体、不同激素配比的比较研究,建立了牛蒡离体培养高效植株再生体系.牛蒡子叶与下胚轴切段在含2.0 m g/L 2,4-D和0.5~2.0 m g/L BA的M S培养基中愈伤组织诱导率可以达到87%~100%;在1.0~3.0 m g/L NAA和0.5~2.0 m g/L BA的M S培养基上通过愈伤组织间接分化或外植体直接分化形成不定芽,其中愈伤组织分化率可达100%;下胚轴的分化率明显高于子叶,在1.0 m g/L NAA和1.0 m g/L BA的M S培养基上下胚轴直接分化率达77.3%.组织学观察发现牛蒡再生有器官发生和体细胞胚发生两种途径.将生长状态良好的不定芽转至含1.0 m g/L IBA和1.0 m g/L NAA的1/2 M S培养基上生根,移栽,成活率达到93.3%.从诱导愈伤组织到组培苗在珍珠岩中过渡成活,大约需要13周.组培苗次年开花并结实,生长形态特征正常. 相似文献