首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 359 毫秒
1.
冯慧  黄原  任轶  冯成利  刘晓农 《生态学报》2014,34(20):5887-5895
林麝(Moschus berezovskii)曾广泛分布于中国,由于盗猎和栖息地缩小,秦岭地区野生种群数量迅速下降,圈养繁殖种群已成立了几十年,但大多数圈养种群的遗传背景不清,种群规模增长非常缓慢。为了给这一物种的保护和管理提供有用的信息,调查了陕西省林麝1个圈养种群3个野生种群线粒体DNA(mt DNA)D-Loop 632 bp片段的遗传多样性和种群结构。在69个个体中其碱基组成为A+T的平均含量63.2%高于G+C含量36.8%,共检测到变异位点171个(约占总位点数的27.05%)。核苷酸多样性(Pi)为0.04424,平均核苷酸差异数(K)为19.908。69个个体分属32个单倍型,单倍型间的平均遗传距离(P)为0.070。32个单倍型构建的NJ系统树聚为3个分支,4个林麝群体中的单倍型是随机分布的。4个群体的平均遗传距离为0.043(标准误SE为0.005),凤县养殖场群体与留坝和陇县群体的亲缘关系较远。单倍型间的平均遗传距离为0.043,可见其遗传分化尚未达到种群分化的水平。结果表明,陕西省林麝群体mt DNA D-loop区序列存在着较丰富的变异和遗传多样性,凤县野生群体和凤县养殖场群体的核苷酸多样性和单倍型多样较高,养殖场种群没有出现近亲繁殖及遗传多样性下降的情况。凤县野生群体和凤县养殖场群体两者遗传分化较小,存在着较高的基因流水平。  相似文献   

2.
The major histocompatibility complex (MHC) is one of the most important elements in immune system for nearly all vertebrates, and is thought to be essential for an organism to recognize foreign molecules. In this study, we investigated the MHC variation in 51 forest musk deer (Moschus berezovskii) collected from three captive populations in Sichuan Province, China. Seventeen haplotypes were isolated from the 51 samples. A total of 51 mutation sites were identified and yielded a nucleotide diversity of 0.056. These haplotype sequences possessed 83 putative amino acid sites, including 24 PBR sites (peptide binding region) and 59 non-PBR sites. Out of 24 PBR sites, 15 codons showed variation (0.625), while 12 codons showed variation (0.203) in 59 non-PBR sites. Non-synonymous substitutions primarily occurred in PBR, with analyses suggesting that the Mobe-DRB gene had undergone strong positive selection during their evolution. Compared with that of some other endangered species, the forest musk deer had relatively high level of MHC diversity. Our results suggested that the MHC diversity characteristic of captive forest musk deer populations might be not responsible for their high morbidity of abscess disease.  相似文献   

3.
The Miyaluo captive forest musk deer population (Sichuan Province, China) is one of the largest captive breeding populations in the world. In order to evaluate the genetic quality and provide available genetic management strategy, seven polymorphism microsatellite loci were applied to assess the genetic variation of the Miyaluo forest musk deer. The results indicated that a total of 168 alleles were detected from these seven microsatellite loci in 361 individuals, and the number of the alleles per locus ranged from 12 to 41 with a mean of 24. The average observed heterozygosity, expected heterozygosity, and PIC were 0.782, 0.854, and 0.837, respectively. Considering the results of the loci Hardy–Weinberg equilibrium test, the comparison of the common allele frequency as well as the private allele between the adults and juveniles, we concluded that the heterozygosity and the genetic diversity of the Miyaluo captive breeding population are increasing due to the input of new individuals from other populations. However, the frequency of some alleles declined sharply, and some were even lost indicating that there is a risk for diversity loss. Thus, we proposed an improved management and breeding strategy for the captive breeding population of the forest musk deer.  相似文献   

4.
Phylogeography and conservation genetics of Eld's deer (Cervus eldi)   总被引:6,自引:0,他引:6  
Eld's deer (Cervus eldi) is a highly endangered cervid, distributed historically throughout much of South Asia and Indochina. We analysed variation in the mitochondrial DNA (mtDNA) control region for representatives of all three Eld's deer subspecies to gain a better understanding of the genetic population structure and evolutionary history of this species. A phylogeny of mtDNA haplotypes indicates that the critically endangered and ecologically divergent C. eldi eldi is related more closely to C. e. thamin than to C. e. siamensis, a result that is consistent with biogeographic considerations. The results also suggest a strong degree of phylogeographic structure both between subspecies and among populations within subspecies, suggesting that dispersal of individuals between populations has been very limited historically. Haplotype diversity was relatively high for two of the three subspecies (thamin and siamensis), indicating that recent population declines have not yet substantially eroded genetic diversity. In contrast, we found no haplotype variation within C. eldi eldi or the Hainan Island population of C. eldi siamensis, two populations which are known to have suffered severe population bottlenecks. We also compared levels of haplotype and nucleotide diversity in an unmanaged captive population, a managed captive population and a relatively healthy wild population. Diversity indices were higher in the latter two, suggesting the efficacy of well-designed breeding programmes for maintaining genetic diversity in captivity. Based on significant genetic differentiation among Eld's deer subspecies, we recommend the continued management of this species in three distinct evolutionarily significant units (ESUs). Where possible, it may be advisable to translocate individuals between isolated populations within a subspecies to maintain levels of genetic variation in remaining Eld's deer populations.  相似文献   

5.
Forest musk deer (Moschus berezovskii) are rare as a result of poaching for musk and habitat loss. Some captive populations of forest musk deer have been established for decades in China. However, little genetic information is available for conservation management. In this paper, genetic variations, population structures, and the genetic bottleneck hypothesis were examined using 11 microsatellite loci from captive populations in Miyalo, Jinfeng and Maerkang in Sichuan Province, China. Estimates of genetic variability revealed substantial genetic variation in the three populations. A total of 142 different alleles were observed in 121 forest musk deer and the effective number of alleles per locus varied from 6.76 to 12.95. The average values of observed heterozygosity, expected heterozygosity, and Nei's expected heterozygosity were 0.552, 0.899 and 0.894 respectively. The overall significant (P < 0.001) deficit of heterozygotes because of inbreeding within breeds amounted to 34.5%. The mean FST (P < 0.001) showed that approximately 90.2% of the genetic variation was within populations and 9.8% was across populations. The UPGMA diagram, based on Nei's unbiased genetic distance, indicated that the three populations were differentiated into two different groups and it agreed with their origin and history. Bottleneck tests indicated that all three populations have undergone a population bottleneck, suggesting a small effective population size. Acknowledging that the genetic structure of populations has crucial conservation implications, the present genetic information should be taken into account in management plans for the conservation of captive forest musk deer.  相似文献   

6.
Chinese water deer is a rare and vulnerable animal in China because of the poaching for medical use and the habitat loss. In this study, the genetic diversity and population genetic structure of 40 Chinese water deer from three populations in Zhoushan Archipelago were investigated with ten highly polymorphic microsatellite loci, including 4 screened from the nuclear DNA in the study, and 6 selected from the literature. According to the results, these captive populations had a higher genetic diversity than other rare cervid species, such as forest musk deer. No signs of inbreeding were detected. Low genetic differentiation among these populations was found. The probable reasons included the isolation by distance, the exchange among islands, or the supplement of the wild Chinese water deer. We proposed the deer raisers to strengthen the exchanges from different islands or mainland, and if possible, some deer would be returned to the wild to expand the wild population.  相似文献   

7.
 The Japanese bitterling Tanakia tanago is an endangered cyprinid species; thus, captive breeding programs are being conducted in various facilities as ex situ conservation. To examine the genetic diversity in one wild and three reared populations, and its changes during the process of captive breeding, sequences of the mitochondrial cytochrome b gene and control region were determined. The wild population, collected in 1993, was monomorphic. Although the reared population that originated from the wild population was almost monomorphic, a rare haplotype, distinct from all others by a relatively large sequence divergence, was also observed. In the other reared populations, some degree of genetic diversity had been maintained. A reared hybrid population, which originated from a mixture of three distinct populations, showed the greatest genetic diversity. These results suggest considerable genetic diversity within and among populations of T. tanago in the past. Although a loss of genetic diversity was observed in some year-classes of reared populations, there was no tendency for genetic diversity to decrease as a result of captive breeding, probably because offspring were obtained from multi-year-class parents in the captive breeding program. Accordingly, this breeding method should be appropriate for conserving the genetic diversity of T. tanago. Received: June 12, 2002 / Revised: December 3, 2002 / Accepted: December 16, 2002  相似文献   

8.
Musk deer are of high conservation priority owing to poaching pressure because of its musk pod. Representation of musk deer status using genetics is poorly documented in India, and it is not confirmed as to how many species of musk deer are present. We characterize for the first time, the genetic diversity of musk deer from Uttarakhand using Cytochrome Oxidase sub-unit (COI) gene (486?bp) and compared with the data available for other species. Results revealed the presence of six haplotypes in the Uttarakhand population amongst 17 sequences. Of these, 12 sequences shared the single haplotype. The intra-species sequences divergence was 0.003–0.017, whereas divergence with other species of musk deer was 0.071–0.081. Bayesian phylogenetic tree revealed that samples from Uttarakhand formed a separate clade with respect to other species of musk deer, whereas three species distributed in China clustered in the same clade and showed low sequences divergence, i.e., 0.002–0.061. Because of different ecomorph reported, we suggest using the barcoding based approach for inter and intra-species distinction and delineating species boundaries across the range for effective conservation. Besides, systematic classification, DNA barcoding would also help in dealing wildlife offence cases for disposal of the legal report in court.  相似文献   

9.
马麝诸种群地理分化初步探讨   总被引:2,自引:0,他引:2  
马麝是我国的一种重要经济动物, 是古北界特有种, 主要分布在我国青藏高原及其临近地区。为保护物种多样性, 本文利用分子生物学技术在分子水平探讨了马麝各地理种群之间遗传多样性问题。用线粒体细胞色素b(Cytochrome b) 的DNA 序列进行系统进化分析显示: 马麝四川种群、甘肃种群和西藏两种群在大约100 万年前具有共同祖先。四川种群最先分化出来, 甘肃马麝与西藏马麝的关系最近, 四川马麝与西藏马麝、甘肃马麝间的关系较远, 甘肃马麝与四川马麝约在80 万年前分化, 而甘肃与西藏察隅和类乌齐两种群马麝间分化时间则分别为34万年和57 万年前, 四川马麝与西藏察隅和类乌齐两种群马麝分化时间在90 万年和100 万年前。结果初步表明分布于甘肃、四川和西藏的马麝种群可能为不同的亚种。  相似文献   

10.
我国麝养殖是解决麝类野生种群保护和中医药麝香来源的关键环节,是极为重要的野生动物养殖业。通过调查得出,截至2011年全国养殖麝已达8400余头,主要分布于四川、陕西等省份;养殖麝种群雄雌比率为1∶0.97,成幼比率为1∶0.53,表明养殖麝种群处于快速增长状态,种群结构基本合理。结合目前麝养殖业资料的调查,分析了养殖规模、养殖技术、种源管理、产业政策对麝养殖产业发展的作用并提出提升麝养殖业发展的对策。  相似文献   

11.
Studies on the genetic diversity and relatedness of zoo populations are crucial for implementing successful breeding programmes. The European wildcat, Felis s. silvestris, is subject to intensive conservation measures, including captive breeding and reintroduction. We here present the first systematic genetic analysis of the captive population of Felis s. silvestris in comparison with a natural wild population. We used microsatellites and mtDNA sequencing to assess genetic diversity, structure and integrity of the ex situ population. Our results show that the ex situ population of the European wildcat is highly structured and that it has a higher genetic diversity than the studied wild population. Some genetic clusters matched the breeding lines of certain zoos or groups of zoos that often exchanged individuals. Two mitochondrial haplotype groups were detected in the in situ populations, one of which was closely related to the most common haplotype found in domestic cats, suggesting past introgression in the wild. Although native haplotypes were also found in the captive population, the majority (68%) of captive individuals shared a common mtDNA haplotype with the domestic cat (Felis s. catus). Only six captive individuals (7.7%) were assigned as wildcats in the STRUCTURE analysis (at K = 2), two of which had domestic cat mtDNA haplotypes and only two captive individuals were assigned as purebred wildcats by NewHybrids. These results suggest that the high genetic diversity of the captive population has been caused by admixture with domestic cats. Therefore, the captive population cannot be recommended for further breeding and reintroduction.  相似文献   

12.
王永奇  盛岩  刘文华  李斐然  唐婕  孟秀祥 《生态学报》2015,35(15):4986-4992
林麝是濒危资源动物,林麝驯养是保育濒危林麝资源及可持续利用麝香的有效方式。基于对2001至2012年间的陕西凤县林麝驯养的监测和调查,分析了其种群动态、性比和年龄结构。结果表明,陕西凤县的林麝驯养在近10余年获得了快速发展,全县共有150余个麝场,麝场数呈指数式增长,增长率达27.33%,但其平均驯养规模无明显增长,平均存栏种群仅为16.38头。凤县的驯养麝种群总体增长近似指数式增长,增长率达27.22%,目前存栏种群已达3712头。区分性别和年龄,各亚群的增长均呈指数式增长,幼年麝的增长率(30.30%)高于成年麝(27.16%),雄麝的增长率(28.30%)高于雌麝(27.78%)。在2001至2012年间,幼麝种群的雌雄性比((102.64±3.15)%,n=12)和成年麝种群的雌雄性比((100.85±2.585)%,n=10)均显著偏雌(P0.01),但幼麝、成年麝种群间的性比差异不显著(P0.05)。在2005年及2010—2012年间,幼麝(0.5岁龄)占种群的比例为31.91%,亚成体麝(1.5岁龄)占种群的比例为21.11%,成麝(2.5—12.5岁龄)比例为42.72%,老年麝(13.5岁龄及以上)仅占种群的4.26%。合并年龄分析,育龄前个体(幼麝和亚成体麝)的平均比例为53.02%,表明凤县驯养林麝属快速增长种群,其增长潜力较大。在林麝驯养实践中,管理部门可制定准入制度或适当重组现有麝场,促进较大的驯养种群构建,并建立通畅的麝香交易渠道,控制林麝种源的过热交易,以利于林麝驯养种群的性比平衡及可持续的繁育、增长。  相似文献   

13.
本文利用13对微卫星标记,对我国3个核心种源地(巴山、秦岭、川西高原)圈养林麝种群进行遗传多样性和遗传结构分析。在167份样品中共检测到142个等位基因(Na),每个位点等位基因数介于7~16,均值为10.92,平均有效等位基因数(Ne)为6.3730,期望杂合度(He)和观测杂合度(Ho)均值分别为0.8302和0.3897。这些圈养林麝种群遗传多样性水平较高,但较低的观测杂合度表明圈养群体存在近交现象。两两群体间的Fst 值和AMOVA分析结果均表明种群之间分化程度不明显。群体遗传结构分析显示,全部样本聚为3个遗传簇(最佳K值=3),其主体与3个地理来源相符,但种群间存在基因渗透现象。本研究中的秦岭种群遗传变异最为丰富,可以作为种质改良的基因池。  相似文献   

14.
研究基于线粒体D-loop区和Cyt b基因部分序列, 分析了广西全州、融水、环江三地禾花鲤(Cyprinus carpio)的遗传结构。在3个群体中共鉴定到19种D-loop与Cyt b序列的组合单倍型, 总的单倍型多样性(Hd)和核苷酸多样性(π)分别为0.916±0.010和0.008±0.004, 禾花鲤线粒体DNA具有单倍型多样性高和核苷酸多样性低的特点。环江群体单倍型多样性最低, 但是核苷酸多样性却最高, 反映了环江群体存在最明显的谱系混杂。分子方差分析(AMOVA)显示遗传变异主要来源于群体内部(71.54%), 禾花鲤三个群体间有显著的遗传分化(Fst=0.285; P<0.01)。系统发育分析表明, 多数样本的线粒体单倍型属于华南鲤(C. carpio rubrefusus)类型, 同时三地禾花鲤中均检测到一定频率的西鲤(C. carpio carpio)或远东鲤(C. carpio haematopterus)类型的线粒体单倍型, 提示禾花鲤可能受到鲤养殖品种的杂交渐渗。研究初步揭示了广西禾花鲤的种质资源现状, 为禾花鲤这一地方特色“稻鱼共作”品种的选育和苗种管理提供了必要的依据。  相似文献   

15.
长吻(鱼危)(Leiocassis longirostris)是中国土著珍稀鱼类。近年来, 由于江河水利工程、环境污染及人类生产活动已经对江河的渔业资源造成了难以逆转的破坏, 长吻(鱼危)的渔业资源已逐渐枯竭。目前, 长吻(鱼危)在四川、广东等地实现适度规模养殖。以四川眉山、湖北石首和安徽淮南3个人工养殖长吻(鱼危)群体及4个长江野生长吻(鱼危)群体(重庆段、武汉段、安庆段和南京段)为实验材料, 利用线粒体DNA (mtDNA)控制区序列作为分子标记对135个个体的遗传结构进行了分析。结果表明, 在790 bp 的同源序列中, 长吻(鱼危) 3个养殖种群共检测到变异位点27个, 占全部序列的3.42%, 66个个体共检测到18种单倍型; 在野生群体中, 69个个体共检测到35个变异位点和36个单倍型, 长吻(鱼危)野生群体平均单倍型多样性和平均核苷酸多样性(Hd=0.9736±0.0070, Pi=0.0087±0.0015)高于长吻(鱼危)养殖群体(Hd=0.8867±0.0013, Pi=0.0056±0.0013); 群体间的遗传分化水平较低(Fst值为0.0014—0.1125)。采用邻接法(NJ法)和统计简约原理对所有单倍型进行系统发育树和统计简约网状图的构建, 结果表明: 各群体内的个体均不能分别构成独立的分支, 而是相互交叉聚在一起。分析结果表明, 长吻(鱼危)养殖群体与野生群体之间的基因交流充分, 未出现遗传分化, 但相对长吻(鱼危)野生群体, 长吻(鱼危)养殖种群多态性偏低。  相似文献   

16.
Across species, diversity at the major histocompatibility complex (MHC) is critical to individual disease resistance and, hence, to population health; however, MHC diversity can be reduced in small, fragmented, or isolated populations. Given the need for comparative studies of functional genetic diversity, we investigated whether MHC diversity differs between populations which are open, that is experiencing gene flow, versus populations which are closed, that is isolated from other populations. Using the endangered ring‐tailed lemur (Lemur catta) as a model, we compared two populations under long‐term study: a relatively “open,” wild population (n = 180) derived from Bezà Mahafaly Special Reserve, Madagascar (2003–2013) and a “closed,” captive population (n = 121) derived from the Duke Lemur Center (DLC, 1980–2013) and from the Indianapolis and Cincinnati Zoos (2012). For all animals, we assessed MHC‐DRB diversity and, across populations, we compared the number of unique MHC‐DRB alleles and their distributions. Wild individuals possessed more MHC‐DRB alleles than did captive individuals, and overall, the wild population had more unique MHC‐DRB alleles that were more evenly distributed than did the captive population. Despite management efforts to maintain or increase genetic diversity in the DLC population, MHC diversity remained static from 1980 to 2010. Since 2010, however, captive‐breeding efforts resulted in the MHC diversity of offspring increasing to a level commensurate with that found in wild individuals. Therefore, loss of genetic diversity in lemurs, owing to small founder populations or reduced gene flow, can be mitigated by managed breeding efforts. Quantifying MHC diversity within individuals and between populations is the necessary first step to identifying potential improvements to captive management and conservation plans.  相似文献   

17.
为揭示北沙柳(Salix psammophila)的遗传多样性、遗传结构及分化特征,利用叶绿体非编码区序列(trnL-trnF和trnD-trnT)对分布于毛乌素沙地和库布齐沙漠的16个北沙柳居群(339个个体)进行了遗传研究,为北沙柳种子资源库遗传管理、遗传改良、遗传育种及品种选育提供理论依据。结果表明:(1)经trnL-trnF和trnD-trnT片段联合比对获得了1811 bp序列,共有12个核苷酸变异位点(8个简约信息位点,4个变异位点),得到16个单倍型。(2)单倍型多样性指数(Hd)为0.737,核苷酸多样性指数(π)为0.00107;且单倍型H3的分布在所有居群中位于单倍型网络图中心,其余单倍型随机分布于各个居群。(3)AMOVA分析表明,北沙柳cpDNA的变异主要来源于居群内(91.16%),居群间遗传分化程度中等(FST=0.08837),各居群间的基因交流非常频繁(Nm=2.58);遗传分化系数NST(0.085)显著大于GST(0.056,0.01相似文献   

18.
小熊猫是亚洲特有的珍稀濒危动物,目前受到栖息地减少、片断化和人类活动干扰等威胁。中国圈养小熊猫已经有60 多年历史,约55 个机构曾经饲养过小熊猫,现今圈养数量有400 多只,评估小熊猫圈养种群的遗传多样性和遗传结构对科学维持圈养种群和保存遗传种质资源意义重大。本研究利用19 个微卫星座位,对中国境内11 个小熊猫圈养种群的116 只个体进行了遗传多样性评估及遗传结构分析。结果显示11 个种群都具有较高的遗传多样性,平均基因丰富度3.505 ± 1.033 (北京)至4.026 ± 1.219 (冕宁),期望杂合度0.631 ± 0.225(黄山)至0.782 ±0.171 (温岭)。其中福州和无锡种群极显著偏离Hardy-Weinberg 平衡。整个圈养群体内各个种群遗传分化系数为0.055,呈显著分化,表明11 个种群遗传分化水平较高。Bayesian 遗传聚类分析将11 个种群聚为三个遗传簇,与野生种群的遗传聚类结果一致。结论:小熊猫圈养种群与野生种群相比,同样具有较高的遗传多样性。因此,圈养小熊猫遗传管理的重点不再是引进野生个体充实圈养种群,应制订科学的繁殖计划,避免近交,从而维持圈养种群的遗传多样性。  相似文献   

19.
Hu J  Fang SG  Wan QH 《Biochemical genetics》2006,44(3-4):161-172
The Chinese water deer (Hydropotes inermis inermis) is endemic to China. Historically, the species was widely distributed, but now, habitat loss and poaching have reduced its range and number drastically. In order to provide useful information for its conservation, we have investigated the genetic diversity and population structure of the Chinese water deer by analyzing the 403 bp fragment of the mitochondrial DNA (mtDNA) control region (D-loop). Eighteen different haplotypes were detected in 40 samples. Overall, Chinese water deer have a relatively high-genetic diversity compared to other rare cervid species, with a haplotype diversity of 0.923+/-0.025 and nucleotide diversity of 1.318 +/- 0.146%. No obvious phylogenetic structure among haplotypes was found for samples of different origin. An analysis of molecular variance (AMOVA) showed significant differentiation between the Zhoushan and the mainland population (F(ST)= 0.088, P < 0.001; Phi( ST ) = 0.075, P = 0.043), which suggests that exchanges of individuals between Zhoushan and the mainland should be avoided. We also recommend that a breeding center be set up for the mainland population.  相似文献   

20.
Xiao CT  Zhang MH  Fu Y  Koh HS 《Biochemical genetics》2007,45(1-2):93-102
Partial sequences of the mitochondrial control region of northeastern China roe deer were analyzed to determine the degree of genetic diversity. Fourteen haplotypes were observed. The haplotype diversity was high (h = 0.872), nucleotide diversity was medium (p i = 0.0108), and the average Tamura–Nei nucleotide distance among them was 1.9%, indicating that genetic diversity of roe deer from northeastern China was relatively high and that the effective population size was large historically. To clarify the northeastern China roe deer's taxonomic status, these 14 haplotypes were compared with 31 haplotypes published in Genbank from Europe, Siberia, and Korea. The average genetic distance between haplogroups of northeastern China and European roe deer (5.8%) was more than twice that between northeastern China and Siberian roe deer (2.7%), indicating sufficient variation to consider roe deer of northeastern China and Siberia as a single species (Capreolus pygargus), distinct from European roe deer (Capreolus capreolus). This is the first presentation of mtDNA data for roe deer in northeastern China, which will be helpful in investigations of genetic diversity and clarifications of the taxonomic status of roe deer in the whole of China.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号