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1.
Effect of Fish Oil and Coconut Oil on Antioxidant Defence System and Lipid Peroxidation in Rat Liver
Massimo D'aquino Paola Corcos Benedetti Maurizio Di Felice Vincenzo Gentili Gianni Tomassi Matilde Maiorino Fulvio Ursini 《Free radical research》1991,12(1):147-152
Diets high in fish oil containing polyunsaturated fatty acids of the n-3 family. have been suggested to decrease the risk of cardiovascular disease. However these lipids are highly susceptible to oxidative deterioration. In order to investigate the influence of n-3 fatty acids on oxidative status, the effect of feeding rats with fish oil or cocunut oil diets was studied by measuring different parameters related to an oxidative free radical challenge. Synthetic diets containing 15% (w/v) fish oil or coconut oil were used to feed growing rats for 4 weeks. As compared to control diet, the fish oil containing diet produced a significant decrease of cholesterol and triglyceride concentration in serum. however there was a significant increase in lipid peroxidation products. In addition, in fish oil fed animals, there was also a decrease in vitamin E and A concentration. Furthermore, the rate of lipid peroxidation in isolated microsomes was three fold higher in rats fed fish oil as compared to rats with coconut oil diet. No significant differences between the two experimental groups were observed in superoxide dismutase (SOD) and phospholipid hydroperoxide glutathione peroxidase (PHGPX) activities. However, there was a decrease in glutathione peroxidase (GPX) activity. These results suggest that fish oil feeding at an amount compatible with human diet, although decreasing plasma lipids, actually challenge the antioxidant defence system, thus increasing the susceptibility of tissues to free radical oxidative damage. 相似文献
2.
Effect of Fish Oil and Coconut Oil on Antioxidant Defence System and Lipid Peroxidation in Rat Liver
《Free radical research》2013,47(1):147-152
Diets high in fish oil containing polyunsaturated fatty acids of the n-3 family. have been suggested to decrease the risk of cardiovascular disease. However these lipids are highly susceptible to oxidative deterioration. In order to investigate the influence of n-3 fatty acids on oxidative status, the effect of feeding rats with fish oil or cocunut oil diets was studied by measuring different parameters related to an oxidative free radical challenge. Synthetic diets containing 15% (w/v) fish oil or coconut oil were used to feed growing rats for 4 weeks. As compared to control diet, the fish oil containing diet produced a significant decrease of cholesterol and triglyceride concentration in serum. however there was a significant increase in lipid peroxidation products. In addition, in fish oil fed animals, there was also a decrease in vitamin E and A concentration. Furthermore, the rate of lipid peroxidation in isolated microsomes was three fold higher in rats fed fish oil as compared to rats with coconut oil diet. No significant differences between the two experimental groups were observed in superoxide dismutase (SOD) and phospholipid hydroperoxide glutathione peroxidase (PHGPX) activities. However, there was a decrease in glutathione peroxidase (GPX) activity. These results suggest that fish oil feeding at an amount compatible with human diet, although decreasing plasma lipids, actually challenge the antioxidant defence system, thus increasing the susceptibility of tissues to free radical oxidative damage. 相似文献
3.
Spinach ( Spinacia oleracea Mill. ) cultivar "Huabo No. 1" was grown in an indoor environment and treated with 13.0 kJ' m-1. d-1 of ultraviolet-B (UV-B 280 to 320 nm) to study the effect of UV-B irradiation on flavonoids and lipid peroxidation in spinach leaves. The results showed that enhanced UV-B irradiation decreased the leaf fresh weight and the content of soluble protein and chlorophyll, and induced large accumulation of UV-absorbing flavonoids in the leaves. UV-B irradiation also promoted the production of superoxide radicals (O2-) and malondialdehyde in spinach leaves. However, the ascorbic acid (ASA) level was decreased under UV-B treatment. It was interesting that high peroxidase (POX), superoxide dismutase (SOD) and catalase (CAT) activities in spinach leaves were induced by UV-B irradiation, the former two being more sensitive. It was suggested that UV-B induced the accumulation of O2- resulting in the lipid peroxidation and in mm inhibiting the growth of spinach. However, the increase of UV-absorbing flavonoids and anti-oxidative enzymes induced by high accumulation of 02- could not reverse the process of UV-B damage. 相似文献
4.
Saadet Gümüşlü Süreyya Bilmen Sarikçiogˇlu Emel Şahin Piraye Yargiçogˇlu Aysel Agˇar 《Free radical research》2013,47(12):1277-1282
The aim of this study was to investigate the influences of different stress models on the antioxidant status and lipid peroxidation (LPO) in erythrocytes of rats. Swiss-Albino female rats (3 months old) were used in this study. Rats were randomly divided into the following four groups; control group (C), cold stress group (CS), immobilization stress group (IS) and cold+immobilization stress group (CS+IS). Control group was kept in an animal laboratory (22 - 2°C). Rats in CS group were placed in cold room (5°C) for 15 min/day for 15 days. Rats in IS group were immobilized for 180 min/day for 15 days. Rats in CS+IS group were exposed to both cold and immobilization stresses for 15 days. At the end of experimental periods, the activities of glucose-6-phosphate dehydrogenase (G-6-PD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and concentration of reduced glutathione (GSH) were measured. LPO was determined by measuring the contents of thiobarbituric acid-reactive substances (TBARS). Cu,Zn-SOD activity and TBARS concentration were increased after cold and immobilization stresses, but CAT and GSH-Px activities and GSH levels were decreased. Immobilization stress decreased the activity of G-6-PD. The activities of G-6-PD, CAT and GSH-Px, and the level of GSH were lower in CS+IS group than in the control group. Cu,Zn-SOD activity and TBARS levels were increased in CS+IS group when compared with the control group. From these findings, three stress models are thought to cause oxidative stress. 相似文献
5.
Casado A Encarnación López-Fernández M Concepción Casado M de La Torre R 《Neurochemical research》2008,33(3):450-458
It has been reported that oxidative stress may play a role in the pathogenesis of dementia of the Alzheimer type (AD) and
the cerebral ischemia which causes vascular dementia (VD). We measured malondialdehyde (MDA) levels and superoxide dismutase
(SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) activities in blood samples from patients
with AD and VD and in healthy non-demented controls (CTR) which similar ages to the patients, in order to evaluate the degree
of oxidative stress in patients with AD and VD. A sample of 150 subjects consisting of 50 patients with AD; 50 patients with
VD and 50 CTR, aged from 65 to 85 years on, was analyzed. Most of the changes observed were in SOD activity and MDA levels. Catalase activity were least affected. Significant differences were observed in SOD and GR activity between males and females in CRT and in patients with AD, but
not in VD. We have found a decrease in antioxidant enzymes activities (SOD, CAT, GPx and GR) in patients with AD and VD and
significant differences were observed between CRT and AD patients for ages from 65 to 74, 75 to 84 and from 85 years to 94 years
in SOD activity and MDA levels (P < 0.001). MDA levels increase with age in VD, AD and CTR. No significant variation with respect to sex were detected, but
significant variations in MDA levels were detected between CRT and patients with VD and AD (P < 0.001). We conclude that oxidative stress plays an important role in the brain damage for both AD and VD, being observed
higher levels of oxidative stress for AD that for VD. 相似文献
6.
Pin Wang Tao Zeng Cui-Li Zhang Xu-Cong Gao Zhen Liu Ke-Qin Xie Zhao-Fu Chi 《Neurochemical research》2009,34(7):1293-1298
Carbon monoxide (CO)-induced delayed neuron damage is the serious complication, but the underlying mechanisms are poorly understood.
This study was designed to investigate the time-dependent changes of the lipid peroxidation (malondialdehyde, MDA) and antioxidative
status (glutathione, GSH; glutathione peroxidase, GSH-Px; glutathione reductase, GR; and anti-reactive oxygen species anti-ROS)
in nerve tissues for the possible mechanisms exploration. Adult rats were treated with CO by peritoneal injection, and sacrificed
after day 0, 1, 3, 7, 14 and 21 of treatment. The results showed that the step-down latency progressively shortened while
the numbers of error increased. Comparing with the level of day 0, MDA levels in serum, cerebral cortex and hippocampus significantly
increased on day 1, 3 and 7. The level of GSH increased firstly but then decreased. The activities of GR, GSH-Px, and anti-ROS
decreased in serum, cerebral cortex and hippocampus of rats after day 1, 3, 7, 14 and 21. Thus, we concluded that CO-mediated
delayed neuron damage might be associated with elevation of lipid peroxidation and reduction of antioxidative status. The
time-dependent changes of lipid peroxidation and antioxidative status in serum, cerebral cortex and hippocampus, at least
in part, are involved in the toxic effects of CO poisoning on neuron. 相似文献
7.
Effect of MPTP and l-Deprenyl on Antioxidant Enzymes and Lipid Peroxidation Levels in Mouse Brain 总被引:2,自引:0,他引:2
Abstract: Excessive free radical formation or antioxidant enzyme deficiency can result in oxidative stress, a mechanism proposed in the toxicity of MPTP and in the etiology of Parkinson's disease (PD). However, it is unclear if altered antioxidant enzyme activity is sufficient to increase lipid peroxidation in PD. We therefore investigated if MPTP can alter the activity of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) and the level of lipid peroxidation. l -Deprenyl, prior to MPTP administration, is used to inhibit MPP+ formation and its subsequent effect on antioxidant enzymes. MPTP induced a threefold increase in SOD activity in the striatum of C57BL/6 mice. No parallel increase in GSH-PX or CAT activities was observed, while striatal lipid peroxidation decreased. At the level of the substantia nigra (SN), even though increases in CAT activity and reduction in SOD and GSH-PX activities were detected, lipid peroxidation was not altered. Interestingly, l -deprenyl induced similar changes in antioxidant enzymes and lipid peroxidation levels, as did MPTP. Taken together, these results suggest that an alteration in SOD activity, without compensatory increases in CAT or GSH-PX activities, is not sufficient to induce lipid peroxidation. 相似文献
8.
Effects of Chlorpromazine on the Activities of Antioxidant Enzymes and Lipid Peroxidation in the Various Regions of Aging Rat Brain 总被引:1,自引:0,他引:1
In this work, the effect of chronic intraperitoneal administration of chlorpromazine (5 and 10 mg/kg) on the antioxidant enzymes superoxide dismutase (SOD), catalase (CA), glutathione reductase (GR), and glutathione peroxidase (GP); lipid peroxidation; and lipofuscin accumulation in the brains of rats ages 6, 9, and 12 months was studied. Chlorpromazine increased the activities of SOD, GR, and GP in particulate fraction from cerebrum, cerebellum, and brain stem in a dose-dependent manner. While GR and SOD associated with soluble fraction increased, GP associated with soluble fraction was not affected. CA did not change after chlorpromazine administration in any regions of the brain of rats from all age groups. Chlorpromazine, thus, had a somewhat different action on antioxidant enzymes in different subcellular fractions. Chlorpromazine inhibited lipid peroxidation, both in vivo and in vitro, and it also inhibited accumulation of lipid peroxidation fluorescent products (lipofuscin), which was studied histochemically and biochemically as well. The data indicate that chlorpromazine inhibition of lipid peroxidation and of accumulation of lipofuscin can result from elevation of the activity of brain antioxidant enzymes. 相似文献
9.
Cadmium (Cd) exposure has been recognized to result in a wide variety of cellular responses, including oxidative stress and
body weight loss. The aim of the present study was to examine the effect of lycopene supplementation on the antioxidant defense
system, lipid peroxidation (LPO) level, nitric oxide (NO), tumor necrosis factor alpha (TNF-α) production, and body weight
in Cd-exposed rats. Animals were divided into four groups (n = 7): control, Cd-treated, Cd plus lycopene-treated, and lycopene-treated. Cadmium (as CdCl2) was administrated orally for 20 days (6.6 mg kg−1 day−1), and lycopene (10 mg kg−1 day−1) was similarly administered. Lycopene administration significantly suppressed Cd-induced LPO in plasma and kidney homogenates.
Lycopene also reversed Cd-decreased body weight compared to the control. Cadmium treatment had diverse effects on the antioxidant
enzyme activities. Although antioxidant superoxide dismutase activity was unchanged, glutathione peroxidase activity was decreased,
and catalase activity was elevated in kidney homogenates of Cd-administrated group. However, lycopene treatment reversed Cd-changed
enzyme activities to the control level. Xanthine oxidase activity and TNF-α concentration were not altered by Cd administration,
indicating that superoxide anion production and inflammation were not stimulated. Cadmium did not change NO levels in kidney
homogenates but decreased those in plasma, and this effect was not prevented by lycopene supplementation. The result suggests
that consumption of adequate levels of lycopene may be useful to prevent heavy-metal-induced LPO and body weight loss. 相似文献
10.
Antioxidant defenses and lipid peroxidation damage in estivating toads, Scaphiopus couchii 总被引:3,自引:0,他引:3
J. E. Grundy K. B. Storey 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(2):132-142
Tissue-specific changes in antioxidant defenses and lipid peroxidation damage were analyzed in spadefoot toads, Scaphiopus couchii, to determine how these responded during estivation, a state of suppressed oxygen consumption. Maximal activities of glutathione-S-transferase, glutathione reductase, glutathione peroxidase, superoxide dismutase and catalase were measured in six organs
from 2-month-estivated toads and compared with activities in animals awakened for 10 days after estivation. Activities of
many enzymes, particularly the glutathione-linked enzymes, were significantly lower in tissues of estivating toads than in
awake toads. This indicates that enzymatic antioxidant defenses are probably modulated in response to the rate of reactive
oxygen species generation in tissues, which is proportional to oxygen consumption. Antioxidant enzyme activities were largely
insensitive to high urea, which accumulates during estivation, but were inhibited by elevated KCl. Levels of reduced glutathione
were also significantly lower in three organs during estivation and all organs, except skeletal muscle, exhibited a higher
oxidized/reduced glutathione ratio, indicating a more oxidized state during estivation. Products of lipid peroxidation (conjugated
dienes, lipid hydroperoxides) were higher in tissues of estivated than control toads, suggesting accumulated oxidative damage
to lipids during dormancy. One enzymatic source of free radical generation, xanthine oxidase, appeared to have little impact
because its activity was detectable only in liver and was significantly lower in estivated toads. The data indicate that both
enzymatic and metabolite antioxidant defenses in toads are adaptable systems that are modulated in estivating versus awake
states.
Accepted: 21 October 1997 相似文献
11.
对钒酸根V(V)与红细胞膜相互作用研究表明V(V)使膜蛋白内源荧光淬灭(KD,37=2.23,KD,20=4.17)和膜巯基含量降低,但对膜脂质过氧化影响较小,提示V(V)主要与膜蛋白作用.与V(V)不同,V(V)与红细胞膜的作用虽使膜蛋白就基含量下降,但不显著,其主要作用是引起膜脂质过氧化. 相似文献
12.
Effects of Echis pyramidum Snake Venom on Hepatic and Renal Antioxidant Enzymes and Lipid Peroxidation in Rats 下载免费PDF全文
Abdulrahman K. Al Asmari Haseeb A. Khan Rajamohammed A. Manthiri Khalid M. Al Yahya Kitab E. Al Otaibi 《Journal of biochemical and molecular toxicology》2014,28(9):407-412
The effects of Echis pyramidum venom (EPV) (0.25, 0.50, and 1.00 mg/kg) on activities of superoxide dismutase (SOD) and catalase (CAT) and levels of thiobarbituric acid reactive substances (TBARS) and total thiols (T‐SH) in liver and kidneys of rats were investigated. EPV significantly and dose dependently decreased the activities of SOD and CAT in livers. Although the kidney SOD and CAT activities were not affected by low and medium doses of EPV, the high dose significantly reduced the activities of these enzymes. Liver and kidney TBARS levels were not affected by the low and medium doses of EPV, whereas the high dose significantly increased the TBARS after 6 h postdosing. There was a significant depletion of T‐SH in liver and kidneys of rats exposed to a high dose of EPV. The acute phase oxidative stress due to an EPV injection points toward the importance of an early antioxidant therapy for the management of snake bites. 相似文献
13.
The Effects of Boron on Arsenic‐Induced Lipid Peroxidation and Antioxidant Status in Male and Female Rats 下载免费PDF全文
Ismail Kucukkurt Sinan Ince Hasan Huseyin Demirel Ruhi Turkmen Erten Akbel Yasemin Celik 《Journal of biochemical and molecular toxicology》2015,29(12):564-571
The aim of the present study was to investigate the possible protective effects of boron, an antioxidant agent, against arsenic‐induced oxidative stress in male and female rats. In total, 42 Wistar albino male and female rats were divided into three equal groups: The animals in the control group were given normal drinking water, the second group was given drinking water with 100 mg/L arsenic, and the third group was orally administered drinking water with 100 mg/kg boron together with arsenic. At the end of the 28‐day experiment, arsenic increased lipid peroxidation and damage in the tissues of rats. However, boron treatment reversed this arsenic‐induced lipid peroxidation and activities of antioxidant enzymes in rats. Moreover, boron exhibited a protective action against arsenic‐induced histopathological changes in the tissues of rats. In conclusion, boron was found to be effective in protecting rats against arsenic‐induced lipid peroxidation by enhancing antioxidant defense mechanisms. 相似文献
14.
The effect of chronic ethanol exposure, in a liquid diet, on lipid peroxidation and some antioxidant systems of rat brain was investigated. Chronic ethanol administration induced a greater susceptibility to iron/ascorbate-induced lipid peroxidation, estimated as thiobarbituric reactive substances (TBARS) production, in the microsomal fraction, but a lower lipid peroxidation in the total homogenate. Glutathione (GSH) levels as well as GSH peroxidase and GSH reductase were unaffected, while the activity of Cu-Zn superoxide dismutase was decreased and that of catalase increased. Lipid peroxidation experiments performed in the presence of some hydroxyl radical scavengers suggested that a greater OH· generation may be responsible of the greater TBARS production in the microsomal fraction of ethanol treated rats; differently, in total homogenate of control and ethanol rats a relationship was found between the redox state of iron and TBARS production, suggesting that the lower lipid peroxidation in treated rats may depend on a different modulation of the iron redox state. 相似文献
15.
Oncü M Kocak A Karaoz E Darici H Savik E Gultekin F 《Biological trace element research》2007,118(3):260-268
This experiment was designed to investigate the histological and lipid peroxidation effects of chronic fluorosis on testes
tissues of first- and second-generation rats. Sixteen virgin female Wistar rats were mated with eight males (2:1) for approximately
12 h to obtain first-generation rats. Pregnant rats were divided into two groups: controls and fluoride-given group, each
of which containing five rats. Pregnant rats in the fluoride-given group were exposed to a total dose of 30 mg/l sodium fluoride
(NaF) in commercial drinking water containing 0.07 mg/l of NaF throughout the gestation and lactation periods. After the lactation
period, the young animals (first generation, F1) were exposed to the same dose of NaF in drinking water for 4 months. At the
end of the 4 months of experimental period, nine randomly chosen male rats (F1) were killed and testes tissues were taken
for histopathological and biochemical analysis. The remaining eight female rats were mated with four males (2:1) for approximately
12 h to obtain second-generation rats. Six female were identified as pregnant and treated with similarly throughout the gestation
and the lactation periods. After the lactation period, the young male animals (second generation, F2) were also treated in
the same way for 4 months. At the end of the 4 months of experimental period, nine randomly chosen male rats (F2) were killed
and testes tissues were collected for histopathological and biochemical analysis. The rats in the control group were applied
the same procedure without NaF administration. In biochemical analysis of the fluoride given F1 and F2 rats, it has been found
that plasma fluoride levels and testes thiobarbituric acid reactive substance levels were significantly increased when compared
with the control group. In F1 and F2 rats, similar histopathological changes were observed. In both groups, spermatogenesis
was severely reduced. Spermatogonia and primary spermatocytes were normal, however, there was a widespread degeneration in
other spermatogenic cell lines of the seminiferous epithelium. The histological structures of the Sertoli and interstitial
Leydig cells were normally observed. It is concluded that chronic fluorosis exposure leads to a remarkable destruction in
testes tissues of F1 and F2 rats via lipid peroxidation.
The study was carried out in Suleyman Demirel University. 相似文献
16.
Ethanol-Induced Oxygen Radical Formation and Lipid Peroxidation in Rat Brain: Effect of Chronic Alcohol Consumption 总被引:9,自引:1,他引:8
Carmina Montoliu Soraya Vallés Jaime Renau-Piqueras Consuelo Guerri 《Journal of neurochemistry》1994,63(5):1855-1862
Abstract: The effect of chronic and in vitro ethanol exposure on brain oxygen radical formation and lipid peroxidation was analyzed. Ethanol induces a dose-dependent increase in lipid peroxidation in brain homogenates. The peroxidative effects of alcohol seem to be related to both cytochrome P450 and the ethanol-inducible form of cytochrome P450 (CYP2E1), because preincubation with metyrapone (an inhibitor of cytochrome P450) or with an antibody against CYP2E1 abolished the ethanol-increased lipid peroxidation. Using the formation of dichlorofluorescein, we also demonstrated that both in vitro and chronic alcohol exposure significantly enhanced the formation of oxygen radical species in synaptosomes. Chronic alcohol treatment also leads to an induction of cytochrome P450 (230%), NADPH cytochrome c reductase (180%), NADPH oxidation (184%), and CYP2E1 in brain microsomes. In addition, this treatment produced a decrease in the GSH/GSSG ratio in brain and significantly enhanced the levels of superoxide dismutase and catalase activities. This mechanism could be involved in the toxic effects of ethanol on brain and membrane alterations occurring after chronic ethanol intake. 相似文献
17.
Vanadium has been recognized as industrial hazards that adversely affect male reproductive systems of humans and animals. However, less information is available concerning the underlying mechanism in the pathogenesis of male reproductive dysfunction. The present study investigated the possible involvement of oxidative stress to induce oxidative deterioration of testicular functions in adult rats. The results of in vitro and in vivo studies demonstrate that vanadium treatment resulted in a significant dose- and time-dependent increase in the testicular lipid peroxidation, marked inhibition in the level of superoxide dismutase and catalase activities, decreased sperm counts, and substantially inhibited the activities of Delta(5)3beta- and 17beta-hydroxysteroid dehydrogenase as well as serum testosterone level. Histopathological examination revealed inhibition of spermatogenesis and the preferential loss of maturing and elongated spermatids along with increased percent of abnormal sperm. Taken together, the results suggest that an increase in free radical formation relative to loss of antioxidant defense system during vanadium exposure may render testis more susceptible to oxidative damage leading to their functional inactivation. Thus the toxic effects of vanadium are cumulative and that vanadium produced damages in testes are dose- and time-dependent. 相似文献
18.
不同浓度维生素C对亚油酸微团的促过氧化和抗过氧化作用 总被引:6,自引:0,他引:6
不同浓度维生素C对亚油酸微团的促过氧化和抗过氧化作用于颖彦(河北医学院附属第二医院病理科石家庄050000)冈田茂(日本冈山大学医学部病理学教室700)关键词亚油酸,脂质过氧化,铁离子,维生素C维生素O(简称Vc)在人体内除了参与激素、神经传导介质和... 相似文献
19.
The effect of temperature on Cyprinus carpio spermatozoa in vitro was investigated with spermatozoa activated at 4, 14, and 24 °C. At 30 s post-activation, motility rate was significantly higher at 4 °C compared to 14 and 24 °C, whereas highest swimming velocity was observed at 14 °C. The thiobarbituric acid-reactive substance (TBARS) content was significantly higher at 14 °C and 24 °C than at 4 °C in motile spermatozoa. No significant differences in catalase and superoxide dismutase activity relative to temperature were observed. This study provides new information regarding effect of temperature on lipid peroxidation intensity and spermatozoon motility parameters in carp. The elevation of TBARS seen at higher temperatures could be due to inadequate capacity of antioxidant enzymes to protect the cell against the detrimental effects of oxidative stress induced by higher temperatures. 相似文献
20.
Andras Nagy Guro Valen Bengt Ek Peter Sellei Per-Ove Sjöquist Jarle Vaage 《Free radical biology & medicine》1998,24(9):1462-1469
We investigated the effect of H290/51, a novel, low-molecular-weight inhibitor of lipid peroxidation, on cardiac ischemia–reperfusion injury. Lactate dehydrogenase (LD) release from cultured cardiomyocytes exposed to 1 h hypoxia and 4 h reoxygenation was measured after pretreatment with different concentrations of H290/51. In another series, Langendorff-perfused rat hearts were exposed to 30 min global ischemia and 60 min reperfusion (n = minimum 10 in each group): 1. Control ischemia–reperfusion. 2. Vehicle throughout the experiment. 3. Vehicle during stabilization, and H290/51 (10−6 mol/l) during reperfusion. 4. H290/51 throughout the experiments. During reoxygenation of isolated cardiomyocytes, H290/51 dose dependently inhibited LD release with an pIC50 value of 7.2 ± 0.4 (mean ± SEM), with 10−6 mol/l as the lowest efficient concentration. In isolated hearts ischemia–reperfusion induced severe reperfusion arrhythmias, reduced left ventricular developed pressure (LVDP) and coronary flow (CF), and increased LV end-diastolic pressure (LVEDP). LD activity in the effluent increased. H290/51 throughout perfusion (group 4) reduced the occurrence of severe reperfusion arrhythmias (p < .0001), attenuated the decrease of LVDP (p < .008), and CF (p < .006), the increase of LVEDP (p < .008), and the release of LD (p < .002). Tissue contents of thiobarbituric acid-reactive substances did not increase during reperfusion in controls, but was reduced in group 4 (p < .004). H290/51 given only during reperfusion (group 3) tended to improve cardiac function, but significantly so only for increase of CF (p < .01). The lipid peroxidation inhibitor H290/51 attenuated cardiac injury induced by ischemia–reperfusion. 相似文献