共查询到20条相似文献,搜索用时 15 毫秒
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Mahfouz MM Li L Piatek M Fang X Mansour H Bangarusamy DK Zhu JK 《Plant molecular biology》2012,78(3):311-321
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An engineered promoter driving expression of a microbial avirulence gene confers recognition of TAL effectors and reduces growth of diverse Xanthomonas strains in citrus 下载免费PDF全文
Deepak Shantharaj Patrick Römer Jose F. L. Figueiredo Gerald V. Minsavage Christina Krönauer Robert E. Stall Gloria A. Moore Latanya C. Fisher Yang Hu Diana M. Horvath Thomas Lahaye Jeffrey B. Jones 《Molecular Plant Pathology》2017,18(7):976-989
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A GATA box in the GATA-1 gene hematopoietic enhancer is a critical element in the network of GATA factors and sites that regulate this gene 总被引:17,自引:0,他引:17 下载免费PDF全文
Nishimura S Takahashi S Kuroha T Suwabe N Nagasawa T Trainor C Yamamoto M 《Molecular and cellular biology》2000,20(2):713-723
A region located at kbp -3.9 to -2.6 5' to the first hematopoietic exon of the GATA-1 gene is necessary to recapitulate gene expression in both the primitive and definitive erythroid lineages. In transfection analyses, this region activated reporter gene expression from an artificial promoter in a position- and orientation-independent manner, indicating that the region functions as the GATA-1 gene hematopoietic enhancer (G1HE). However, when analyzed in transgenic embryos in vivo, G1HE activity was orientation dependent and also required the presence of the endogenous GATA-1 gene hematopoietic promoter. To define the boundaries of G1HE, a series of deletion constructs were prepared and tested in transfection and transgenic mice analyses. We show that G1HE contains a 149-bp core region which is critical for GATA-1 gene expression in both primitive and definitive erythroid cells but that expression in megakaryocytes requires the core plus additional sequences from G1HE. This core region contains one GATA, one GAT, and two E boxes. Mutational analyses revealed that only the GATA box is critical for gene-regulatory activity. Importantly, G1HE was active in SCL(-/-) embryos. These results thus demonstrate the presence of a critical network of GATA factors and GATA binding sites that controls the expression of this gene. 相似文献
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