共查询到20条相似文献,搜索用时 15 毫秒
1.
Shedding of airway epithelial cells is a common finding in asthma. In this study, the attachment of the airway epithelial cells to the basal lamina (BL) was investigated by transmission electron microscopy (TEM) of biopsies from patients with atopic asthma and healthy controls. The following parameters were quantitatively determined: the height of the epithelium and of the columnar cells, the number of basal cells per 100 microm of basal lamina, the contact surfaces of basal cells or columnar cells with the basal lamina, and between basal cells and columnar cells. In order to compare the quantitative method with previous literature data, measurements were also carried out on rat airway epithelium. Compared to the rat, the columnar cell height in the human is increased, basal cells are smaller, and there is a larger contact area between basal cells and basal lamina, as well as between basal and columnar cells. The contact area between columnar cells and basal lamina is hence less in the human airway. The contact area between columnar cells and basal lamina in asthmatics is significantly less than in healthy controls, due to larger intercellular spaces. It is concluded that attachment of columnar cells to the basal lamina occurs mainly indirectly, via desmosomal attachment to basal cells, and that direct attachment of columnar cells to the basal lamina is weakened in asthmatics. 相似文献
2.
Radial Columnar Patches in the Chimeric Cerebral Cortex Visualized by Use of Mouse Embryonic Stem Cells Expressing β-Galactosidase 总被引:1,自引:1,他引:0
Norio Nakatsuji Yuzo Kadokawa Hirofumi Suemori 《Development, growth & differentiation》1991,33(6):571-578
Presumed radial migration of neuroblasts from the ventricular to pial surface during formation of the cerebral cortex predicts radial columnar patches in chimeric brains. Lack of adequate cell marker for neurons, however, has hindered such chimera analysis. We used a mouse embryonic stem cell line expressing β-galactosidase gene to produce chimeric brains. Patches of the labeled cells were examined by whole mount staining and also by computer-assisted three-dimensional reconstruction from serial paraffin sections. Our study revealed presence of coherent radial columnar patches in the prenatal cerebral cortex, thus giving a direct evidence for the radial migration of neurons. These columnar patches were less clear in adult brains, suggesting cell mixing during later development and maturation. 相似文献
3.
During neural tube closure, specialized regions called hinge points (HPs) display dynamic and polarized cell behaviors necessary for converting the neural plate into a neural tube. The molecular bases of such cell behaviors (e.g. apical constriction, basal nuclear migration) are poorly understood. We have identified a two-dimensional canonical BMP activity gradient in the chick neural plate that results in low and temporally pulsed BMP activity at the ventral midline/median hinge point (MHP). Using in vivo manipulations, high-resolution imaging and biochemical analyses, we show that BMP attenuation is necessary and sufficient for MHP formation. Conversely, BMP overexpression abolishes MHP formation and prevents neural tube closure. We provide evidence that BMP modulation directs neural tube closure via the regulation of apicobasal polarity. First, BMP blockade produces partially polarized neural cells, which retain contact with the apical and basal surfaces but where basolateral proteins (LGL) become apically localized and apical junctional proteins (PAR3, ZO1) become targeted to endosomes. Second, direct LGL misexpression induces ectopic HPs identical to those produced by noggin or dominant-negative BMPR1A. Third, BMP-dependent biochemical interactions occur between the PAR3-PAR6-aPKC polarity complex and phosphorylated SMAD5 at apical junctions. Finally, partially polarized cells normally occur at the MHP, their frequencies inversely correlated with the BMP activity gradient in the neural plate. We propose that spatiotemporal modulation of the two-dimensional BMP gradient transiently alters cell polarity in targeted neuronal cells. This ensures that the neural plate is flexible enough to be focally bent and shaped into a neural tube, while retaining overall epithelial integrity. 相似文献
4.
Growth and differentiation of the larval midgut epithelium during molting in the moth, Manduca sexta
The number of epithelial cells comprising larval midgut of the tobacco hornworm moth, Manduca sexta increases 200-fold in development from the first to the fifth instar. We have examined larvae periodically before and during molting to follow epithelial cell proliferation and differentiation. The midgut epithelium in Manduca sexta consists predominantly of columnar and goblet cells. These are arranged in a characteristic pattern with each goblet cell surrounded by a single layer of 4-6 columnar cells (Hakim et al., (1988)). While undifferentiated basal stem cells are infrequently seen in intermolt larvae, just prior to the period when external signs of molting are visible, their number increases and mitotic figures become common. Proliferation continues for several hours and then these stem cells differentiate following a pattern similar to that seen during embryogenesis (Hakim et al., (1988)). Here, however, the newly differentiating cells become intercalated among the mature differentiated cells already present in the epithelium. Since the pattern of individual goblet cells surrounded by a reticulum of columnar cells is maintained after the addition of new cells, the midgut epithelium of molting larvae appears to be a useful model for studying pattern formation in development. 相似文献
5.
For the first 9 days of gestation, opossum embryos float in uterine secretions, separated from maternal tissues by a shell membrane. Each embryo is part of the wall of its hollow embryonic sphere. By the 10th day of development, the embryo becomes enveloped by both the amnion and yolk-sac. The yolk-sac consists of vascular and non-vascular portions and, together with the surrounding trophectoderm (trophoblast), forms the yolk-sac placenta of the opossum: the allantois does not contribute to formation of the placenta. The vascular portion of the yolk-sac placenta establishes an intimate relationship with the uterine epithelium soon after loss of the shell membrane. The yolk-sac placenta is non-invasive. Cells of the trophoblast exhibit numerous microvilli, an apical endocytic complex and the lateral and basal cell membrane are elaborately folded. These features suggest a cell that is active in the transport of materials. Junctional complexes between cells of the trophoblast and uterine epithelium were not observed. The uterine epithelium changes from ciliated pseudostratified columnar with few infoldings of lateral and basal cell membranes, to non-ciliated simple columnar in which these membranes show elaborate infoldings. The cells show numerous inclusions and mitochondria are polarized to the basal half of the cell. These features suggest a cell that also is active in the transport of materials. 相似文献
6.
Yunfei Lee Miaoxing Wang Kousuke Imamura Makoto Sato 《Development, growth & differentiation》2023,65(1):37-47
The Drosophila visual center shows columnar structures, basic structural and functional units of the brain, that are shared with the mammalian cerebral cortex. Visual information received in the ommatidia in the compound eye is transmitted to the columns in the brain. However, the developmental mechanisms of column formation are largely unknown. The Irre Cell Recognition Module (IRM) proteins are a family of immunoglobulin cell adhesion molecules. The four Drosophila IRM proteins are localized to the developing columns, the structure of which is affected in IRM mutants, suggesting that IRM proteins are essential for column formation. Since IRM proteins are cell adhesion molecules, they may regulate cell adhesion between columnar neurons. To test this possibility, we specifically knocked down IRM genes in columnar neurons and examined the defects in column formation. We developed a system that automatically extracts the individual column images and quantifies the column shape. Using this system, we demonstrated that IRM genes play critical roles in regulating column shape in a core columnar neuron, Mi1. We also show that their expression in the other columnar neurons, Mi4 and T4/5, is essential, suggesting that the interactions between IRM proteins and multiple neurons shape the columns in the fly brain. 相似文献
7.
The development of ciliated and mucus cells from basal cells in hamster tracheal epithelial cell cultures 总被引:3,自引:0,他引:3
Hamster tracheal epithelia consist of three cell types: ciliated, mucus and basal cells. Autoradiographic data from several studies suggest that either basal or non-ciliated columnar cells may serve as stem cells for regeneration of lost or damaged ciliated and mucus cells. The objective of the present study was to examine the role of basal cells in the formation of ciliated and mucus cells in hamster tracheal epithelial (HTE) cell cultures via tritiated thymidine ([3H]-TdR) autoradiography. When 3 day cultures were pulsed with [3H]-TdR for 6 hr and incubated for 2 additional days in non-radioactive media (5 day total) label was present in the nuclei of basal and columnar epithelial cells suggesting that the labeled columnar cells may be derived from basal cells. However, the morphological reorganization occurring during this 2 day interval may create difficulties in this interpretation. Since these morphological changes are minimal during the 6 day to 8 day in vitro period, 6 day HTE cultures were pulsed with [3H]-TdR for 6 hr and incubated for 2 additional days in non-radioactive media (8 day total), and examined to further study the fate of labeled basal cells during this period. Analysis of these 8 day cultures revealed that labeled nuclei were present in both basal cells and adjacent ciliated and mucus cells. These results do not exclude the possibility of non-basal cell origin of ciliated and mucus cells in other systems but suggest that, at least in HTE cultures, undifferentiated basal cells have the ability to develop into ciliated and mucus cells. 相似文献
8.
The bovine ejaculatory duct is lined by a pseudostratified columnar epithelium. Two cell types are present: small basal cells and columnar principal cells in different functional states. The basal cells are able to accumulate lipid material. The principal cells are observed in a less active state and in a state of either increased endocytosis and fluid uptake or active spermiophagy. Endocytotically active cells are characterized by an apical brush border and a system of microvesicles, multivesicular bodies and lysosomal dense bodies. Cells involved in phagocytosis of spermatozoa are mostly provided with a smooth apical border, an expanded Golgi apparatus, many phagocytic vacuoles and condensing phagolysosomes. 相似文献
9.
10.
The optic vesicle develops as an evagination of the cephalic neural folds. We have examined the early development of the optic vesicle in Swiss Webster mice using correlated transmission electron microscopy (TEM), scanning electron microscopy (SEM), light microscopic (LM) measurements of cell shape changes, immunohistochemical localization of basal lamina (BL) components (type IV collagen, laminin and heparan sulphate proteoglycan (HSPG)) and ultrastructural analysis of the BL. Like the neuroepithelium in other regions, the low columnar cells of the neural plate in the future optic vesicle region become high columnar, then wedge shaped following constriction of the cell apices to form the C-shaped vesicle. In this region, the cells elongate 2 times their initial height before the neural tube closes, then shorten 20% as the vesicle is completed. Cell apices decrease in width by about one half during vesicle formation. Deposition of BL components was initially even, with type IV collagen and laminin reduced in deposition in regions of outpouching. At later stages the linear, even distribution of all four components was re-established. Ultrastructural analysis confirmed the BL discontinuity and re-establishment and correlated the observed cell shaping alterations with apparent increases in the number of microtubules (during elongation) and microfilaments (during apical constriction). The number of apical intercellular junctions also appeared to increase in number during optic vesicle formation, possibly providing stability and coordination to the evagination process. 相似文献
11.
ACTIVE TRANSPORT BY THE CECROPIA MIDGUT : II. Fine Structure of the Midgut Epithelium 总被引:4,自引:4,他引:0
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A morphological basis for transcellular potassium transport in the midgut of the mature fifth instar larvae of Hyalophora cecropia has been established through studies with the light and electron microscopes. The single-layered epithelium consists of two distinct cell types, the columnar cell and the goblet cell. No regenerative cells are present. Both columnar and goblet cells rest on a well developed basement lamina. The basal portion of the columnar cell is incompletely divided into compartments by deep infoldings of the plasma membrane, whereas the apical end consists of numerous cytoplasmic projections, each of which is covered with a fine fuzzy or filamentous material. The cytoplasm of this cell contains large amounts of rough endoplasmic reticulum, microtubules, and mitochondria. In the basal region of the cell the mitochondria are oriented parallel to the long axes of the folded plasma-lemma, but in the intermediate and apical portions they are randomly scattered within the cytoplasmic matrix. Compared to the columnar cell, the goblet cell has relatively little endoplasmic reticulum. On the other hand, the plications of the plasma membrane of the goblet cell greatly exceed those of the columnar cell. One can distinguish at least four characteristic types of folding: (a) basal podocytelike extensions, (b) lateral evaginations, (c) apical microvilli, and (d) specialized cytoplasmic projections which line the goblet chamber. Apically, the projections are large and branch to form villus-like units, whereas in the major portion of the cavity each projection appears to contain an elongate mitochondrion. Junctional complexes of similar kind and position appear between neighboring columnar cells and between adjacent columnar and goblet cells as follows: a zonula adherens is found near the luminal surface and is followed by one or more zonulae occludentes. The morphological data obtained in this study and the physiological information on ion transport through the midgut epithelium have encouraged us to suggest that the goblet cell may be the principal unit of active potassium transport from the hemolymph to the lumen of the midgut. We have postulated that ion accumulation by mitochondria in close association with plicated plasma membranes may play a role in the active movement of potassium across the midgut. 相似文献
12.
Andreas Bubel 《Tissue & cell》1983,15(3):457-476
The structure of the organic material and inorganic elements of the opercular plate and associated cells in the serpulid annelid, Pomatoceros lamarckii Quatrefages, have been described by transmission and scanning electron microscopy. After decalcification the organic material of the opercular plate was found to consist of three major structurally different components, an outer, thin, electron-dense layer, parallel rows of rectangular profiles partitioned into large units by cross-walls, and layers of orthogonally arranged fibres. The inorganic aragonite components were found, in contrast, to consist of two structurally different elements namely, highly ordered crystals with a prismatic-like morphology and smaller needle-like crystallites. Two morphologically distinct cell types, columnar opercular rim and cuboidal opercular plate cells, are responsible for the formation of the opercular plate. Both possess membrane-bound bodies containing filamentous material. However, in addition, membrane-bound bodies, containing calcium carbonate crystals, are found in some cells. Such bodies are seen to be closely related to the Golgi system. Based on the cytoarchitecture of the cells, the mechanisms involved in the formation and calcification of the opercular plate are discussed. 相似文献
13.
A A Rutten J P Bruyntjes F C Ramaekers 《Virchows Archiv. B, Cell pathology including molecular pathology》1988,56(2):103-110
Using immunohistochemical techniques, the keratin expression patterns in basal and columnar cells (mucus-producing and ciliated cells) were investigated in tracheal organ cultures. Tracheas were from either hamsters fed a control diet or from hamsters fed a vitamin A-deficient diet; tracheas from the latter group were treated in vitro with all-trans retinol. In tracheas from hamsters fed a control diet, basal cells generally reacted with the RCK102 antibody and columnar cells with the RGE53 and the HCK19 antibodies, and both basal and columnar cells were recognized by the RCK105 antibody. The squamous cell cytokeratin 10 (detected by the RKSE60 antibody) was not expressed in cultured tracheas from hamsters fed a normal or a vitamin A-deficient diet. In the course of the in vitro period a number of keratins were "switched on" or "switched off" in both basal and columnar cells. In tracheas from vitamin A-deprived hamsters the RCK102 antibody clearly recognized basal cells and cigarette smoke condensate-induced proliferating basal cells, whereas the RGE53 antibody reacted with mucus-producing and ciliated cells. During organ culture foci of columnar epithelial cells expressed basal cell properties (detected with the RCK102 antibody) after all-trans retinol treatment and were found negative for the RGE53 antibody. Furthermore, it appeared that the RGE53-negative columnar cells contained periodic acid-Schiff-positive mucous granules. These findings indicate that basal cells may differentiate into columnar cells. Tracheal epithelium did not appear to co-express vimentin next to keratins during organ culture, which may be due to the intact three-dimensional organization present in these organ cultures. 相似文献
14.
The general structure of the skin of the Lesser octopus, Eledone cirrhosa is described. The component regions and cell types being (1) the epidermis, composed of columnar cells with a border of microvilli on their external surface, mucous cells and secretory cells of an unknown type, abutting a non cellular basal lamina; (2) the dermis, containing the elements of the colour change system, vascular and neural processes and an a cellular matrix.
A sequential, morphological study of wound closure revealed three major mechanisms in the healing response; muscular contraction, a dermal cellular reaction and epidermal migration. The process of wound closure in Eledone is significantly slower than that seen, for example, in teleost fish (30 h rather than 30 min for the initiation of epidermal migration). The significance of these results in relation to the osmotic balance between seawater and the internal milieu of Eledone is discussed, together with their relevance to resistance against infection by pathogens. 相似文献
A sequential, morphological study of wound closure revealed three major mechanisms in the healing response; muscular contraction, a dermal cellular reaction and epidermal migration. The process of wound closure in Eledone is significantly slower than that seen, for example, in teleost fish (30 h rather than 30 min for the initiation of epidermal migration). The significance of these results in relation to the osmotic balance between seawater and the internal milieu of Eledone is discussed, together with their relevance to resistance against infection by pathogens. 相似文献
15.
Primary mesenchyme cell (PMC) migration during PMC-ring pattern formation was analyzed using computer-assisted time-lapse video microscopy in spread embryos (2D-embryo) of the sea urchin, Mespilia globulus , and a computer simulation. The PMC formed a near normal ring pattern in the 2D-embryos, which were shown to be an excellent model for the examination of cell behavior in vivo by time-lapse computer analysis. The average migration distance of the ventro-lateral PMC aggregate-forming cells (AFC) and that of the dorso-ventral PMC cable-forming cells (CFC) showed no significant difference. All PMC took a rather straightforward migration path to their destinations with little lag time after ingression. This in vivo cell behavior fitted well to a computer simulation with a non-diffusable chemotaxis factor in the cyber-cell migration field. This simulation suggests that PMC recognize their destination from a very early moment of cell migration from the vegetal plate, and implicates that a chemoattractive region is necessary for making the PMC migration pattern. The left- and right-lateral AFC and dorso and ventral CFC were each derived from an unequally divided one-quarter segment of the vegetal plate. This suggests that AFC and CFC have a distinctive ancestor in the vegetal plate, and the PMC are a heterogeneous population at least in terms of their destination in the PMC-ring pattern. 相似文献
16.
Ultrastructural features of rectal epithelium of the mouse during the early phases of migration to repair a defect 总被引:1,自引:0,他引:1
R C Buck 《Virchows Archiv. B, Cell pathology including molecular pathology》1986,51(4):331-340
Observations were made by scanning and transmission electron microscopy on the migrating epithelial cells of the mouse rectum at intervals up to 24 h after stripping the epithelium off the mucosa. Resurfacing of the denuded basal lamina proceeded by the centrifugal migration of the columnar cells of the crypts. Changes in these cells occurred very rapidly. In less than 20 min a flat leading lamella developed and extended out on the basal lamina. The leading lamella could be recognized easily in scanning electron micrographs by the absence of microvilli, although these were retained on the cell body, gradually getting less regular and sparser than normal. Many zeiotic blebs appeared on the free margin of these cells. The features of migrating epithelium which are displayed in the in vivo repair of rectal mucosa are shared with migrating epithelia cultured in vitro. Goblet cells appeared not to be active in resurfacing the lesions. They disappeared from the surface epithelium, but were evident again by 18 and 24 h. The method of producing these lesions can also be used to study the cells that are removed. 相似文献
17.
Immunoreaction to TGF-alpha was limited to the basal epithelial cells of focal areas in the normal prostates. In benign prostatic hyperplasia (BPH) the immunostained areas were more widespread and immunolabelling was observed in both basal and columnar (secretory) cells of the epithelium. Some cells in the connective tissue stroma were also stained. In prostatic adenocarcinoma, epithelial immunostaining was even more extensive and intense than in BPH, and some stromal cells were also stained. Epidermal growth factor (EGF) immunostaining was only present in some basal cells in normal prostates. In BPH, this immunoreaction was strong in the basal cells and even stronger in the secretory cells. In prostatic cancer, the intensity of epithelial cell immunoreactivity was intermediate between that of normal prostates and that of BPH specimens. EGF-receptor immunostaining was focal and located in the basal cells in normal prostates. In BPH, labelling was also localized in basal cells but extended to wider areas. Some stromal cells appeared weakly labelled. In the prostatic carcinoma, both basal and columnar cells appeared stained and the number of immunolabelled stromal cells was higher than in BPH. The results presented suggest that, in normal conditions, EGF and TGF-alpha act as autocrine growth factors for the basal cells of the prostatic epithelium. In BPH this action is maintained and, in addition, the columnar cells start to secrete both factors which are bound by the basal cell receptors, giving rise to a paracrine regulation which probably overstimulates basal cell proliferation. In prostatic carcinoma, besides these regulatory mechanisms, the acquisition of EGF-receptors by the secretory cells develops an autocrine regulation which might induce their proliferation. 相似文献
18.
A A Rutten J P Bruyntjes F C Ramaekers 《Virchows Archiv. B, Cell pathology including molecular pathology》1988,56(2):111-117
Keratin expression in hamster tracheal epithelium was investigated during organ culture in serum-free, hormone-supplemented medium using monospecific monoclonal antibodies. Generally, tracheal basal cells expressed keratins detected by antibodies RCK102 and RCK103, while columnar epithelial cells were stained positively by RGE53, RCK103, RCK105 and HCK19. Metaplastic squamous cell foci reacted with antibodies RKSE60, RCK103 and HCK19. Early metaplastic alterations were more clearly RKSE60-positive than the mature lesions. In the vitamin A-depleted tracheas basal cells were clearly RCK102-positive. Superficial cells in the central part of areas of squamous metaplasia induced by cigarette smoke condensate expressed the basal cell keratins, and were negative for the columnar cell keratin 18 detected by the RGE53 antibody. This finding suggests that in cigarette smoke condensate-induced squamous metaplasia basal cells play an important role. The mucus-producing cells at the edges of metaplastic squamous cell foci expressed the keratins specific to columnar cells. Cigarette smoke condensate exposure accelerated epithelial keratinization compared to the vitamin A-depleted epithelium. It was concluded that not only small mucous granule cells, but also basal cells are involved in the development and maintenance of induced squamous metaplasia in tracheal epithelium. Furthermore, in vitro vitamin A-depleted epithelium did not coexpress vimentin in addition to the different keratins. 相似文献
19.
Migratory behavior of cells on embryonic retina basal lamina 总被引:1,自引:0,他引:1
In order to study cell translocation in vitro on a physiological substrate a novel cell migration assay was developed using the inner limiting membrane of the avian embryonic retina. The matrix sheet consists of a laminin-rich basal lamina covered by a dense layer of neuroepithelial endfeet. The retina basal lamina does not contain fibronectin. Cells translocating on this substrate displace the neuroepithelial endfeet, leaving behind tracks in the endfeet monolayer. Motility of cells and the relative forward to lateral migration can be quantitated by measuring lengths, widths, and areas of the tracks. Using this assay system, the conditions and patterns of cell migration for a variety of cells have been examined. In the absence of serum all cell types show only minor migratory activity and addition of serum to the culture medium always enhances the rate of cell migration in a saturable, dose-response manner. The serum cannot be replaced by fibronectin or vitronectin (serum spreading factor). For maximum cell migration, serum has to be constantly present in the medium; however, 58% cell migration is obtained in serum-free medium when the matrix is preincubated with serum. According to the area and linearity of the tracks, the migratory behavior of the different cells can be classified into three groups: (i) fibroblasts and the nonpigmented Bowes melanoma cells form straight and long tracks; (ii) glioma, sarcoma, and carcinoma cells from straight but short tracks, and (iii) neuronal tumor cells, epithelial cells, and pigmented B16 melanoma cells form wide and short tracks. Comparative studies with low and high metastatic clones of tumorgenic cell lines show that migratory activity and metastatic potential of cells do not necessarily correlate. Finally, we show that fibroblasts deposit fibronectin fibrils on their paths as they migrate on the basal lamina. Fibronectin trails are also seen when fibroblasts are cultured on plain basal laminae that are pretreated with detergent to remove the endfeet monolayer. Likewise, when fibroblasts are cultured in the presence of antifibronectin antibodies, the fibronectin secreted by cells is detectable. Due to antibody treatment the cellular fibronectin is precipitated and its normal fibril formation is inhibited; however, the translocation of fibroblasts is not impaired. 相似文献
20.
Delbert R. Dorscheid Amber E. Conforti Kimm J. Hamann Klaus F. Rabe Steven R. White 《The Histochemical journal》1999,31(3):145-151
Glycosylated structures on the cell surface have a role in cell adhesion, migration, and proliferation. Repair of the airway epithelium after injury requires each of these processes, but the normal cell surface glycosylation of non-mucin producing airway epithelial cells is unknown. We examined cell surface glycosylation in human airway epithelial cells in tissue sections and in human airway epithelial cell lines in culture. Thirty-eight lectin probes were used to determine specific carbohydrate residues by lectin-histochemistry. Galactose or galactosamine-specific lectins labeled basal epithelial cells, lectins specific for several different carbohydrate structures bound columnar epithelial cells, and fucose-specific lectins labeled all airway epithelial cells. The epithelial cell lines 1HAEo– and 16HBE14o– bound lectins that were specific to basal epithelial cells. Flow cytometry of these cell lines with selected lectins demonstrated that lectin binding was to cell surface carbohydrates, and revealed possible hidden tissue antigens on dispersed cultured cells. We demonstrate specific lectin-binding patterns on the surface of normal human airway epithelial cells. The expression of specific carbohydrate residues may be useful to type epithelial cells and as a tool to examine cell events involved in epithelial repair. 相似文献