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板齿鼠线粒体DNA的研究 总被引:4,自引:0,他引:4
本文应用ApaI、BamHI、BclI、BglI、ClaI、EcoRI、EcoRV、HindII、PstI、PvuII、SacI、ScaI和XbaI等13种限制性内切酶对板齿鼠线粒体DNA(mtDNA)进行限制性片段长度多态(RFLP)分析,并用双酶解法构建其限制性内切酶图谱。结果表明板齿鼠存在3种mtDNA单倍型,可通过限制酶PvuII、HindII和ApaI区分,呈现DNA多态性和种内遗传变异。与小家鼠、褐家鼠mtDNA限制性片段的数据相比较,板齿鼠和这两种鼠mtDNA存在明显差异。板齿鼠mtDNA限制性内切酶图谱的建立,为进一步系统研究鼠科动物的遗传分化提供了依据。 相似文献
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中国牦牛线粒体DNA多态性及遗传分化 总被引:24,自引:3,他引:24
用20种限制性内切酶分析了我国5个牦牛群体90个个体的限制性片段长度多态性,其中AvaI,AvaII、BglII、EcoRI.HindIII、HpaI6个酶切类型具有多态性,共发现5种mtDNA单倍型,每种单倍型中检出50-55个位点,并利用双酶切制定出其物理图谱。我国牦牛群体mtDNA多样度HT为0.1065,群体内的平均一致性概率为0.8966,表明我国牦牛群体mtDNA多态性较贫乏,群体间的平均净遗传距离Pet为0.000201,群体基因分化系数Gst为0.0291,我国牦牛群体mtDNA变异只有2.91%来自群体间的差异,群体间的分化程度较低。并根据报道,比较了牦牛和其他家养牛种的mtDNA遗传分化,估计出牦牛和普通牛、瘤牛的分化时间大约分别在1.1-2.2百万年和1.01-1.02百万年之间。 相似文献
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树鼠mtDNA限制性内切酶图谱及其与小家鼠褐家鼠的新缘关系 总被引:3,自引:1,他引:3
本实验用Apa I,AVa I,Bam HI<BclI,Cla I,Eco RIEco RV,Hpa I,Pst I,Pvu Ⅱ,ScaI,XbaI等13种限制性内切酶分析树鼠的mtDNA限制性片段长度多态性,并用双酶解法构建了其中8种酶的限制性内切酶图谱。根据限制性片段差异法和分子钟,计算并讨论树鼠和小家鼠、褐家鼠的mtDNA遗传距离和亲缘关系。结果表明树鼠与褐家鼠的关系较接近,两者的分歧时间在 相似文献
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山羊品种间线粒体DNA限制性片段长度多态性研究 总被引:26,自引:2,他引:24
本试验利用ApaⅠ、BamHⅠ、BclⅠ、BglⅠ、BglⅡ、ClaⅠ、EcoRⅠ、EcoRⅤ、HaeⅡ、HindⅢ、KpnⅠPstⅠ、PvuⅡ、SacⅠ、SalⅠ、SmaⅠ和XhoⅠ计18种限制性内切酶,研究了来自欧洲,非洲及国内的5个山羊品种共计33只个体的mtDNA,共检测了27处限制性态型,可归结为8种单倍型。结果表明,国内2个百品种的基本单倍型为BamHⅠ-A,BclⅠ-B、ClaⅠ-A 相似文献
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隼形目鹰科四种鸟类线粒体DNA研究 总被引:5,自引:0,他引:5
本实验采用ApaI,fBamHI,BgiII,EcoRI,EcoRV,HindⅢHpaI,KpnI,PstI,PruII,SalI,ScaI,XbaI和XhoI14种限制性内切酶,对鹰科4种鸟类即雀鹰(Accipiternisus),松雀鹰(A.virgatus)苍鹰(A.gentilis)和灰险鹰(Buftaturindicus)mtDNA限制性片段长度多态分析。结果表明,4种鸟类中雀鹰和松1主 相似文献
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树鼠mtDNA限制性内切酶图谱及其与小家鼠褐家鼠的亲缘关系 总被引:1,自引:1,他引:0
本实验用ApeⅠ,AvaⅠ,BamHⅠ,BclⅠ,BglⅠ,ClaⅠ,EcoRⅠ,EcoRV,HpaⅠ,PstⅠ,PvuⅡ,ScaⅠ,XbaⅠ等13种限制性内切酶分析树鼠(Chiromyscuschiropes)的mtDNA限制性片段长度多态性,并用双酶解法构建了其中8种酶的限制性内切酶图谱。根据限制性片段差异法和分子钟,计算并讨论树鼠和小家鼠(Musmusculus)、褐家鼠(Rattusnorvegicus)的mtDNA遗传距离和亲缘关系。结果表明树鼠与褐家鼠的关系较接近,两者的分歧时间在距今1500─2000万年前,即处于中新世早中期。 相似文献
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海南黄牛和徐闻黄牛线粒体DNA的多态性及其品种分化关系 总被引:10,自引:1,他引:9
本文以ApaⅠ、AvaⅠ、BamHⅠ、BglⅠ、BglⅡ、DraⅠ、EcoRⅠ、EcoRⅤ、HindⅢ、HpaⅠ、PstⅠ、SalⅠ、ScaⅠ和XhoⅠ等14种限制性内切酶分析来自海南岛的海南黄牛和雷州半岛的徐闻黄牛的线粒体DNA限制性片段长度多态性(mtDNAARFLP)。结果只有一种限制性内切酶(SalⅠ)在海南黄牛中检测到多态性,并且其中的C型(15.0,1.3)尚未见报道。我们的结果还显示,两个品种6个个体的mtDNA基因单倍型全部表现为A型,即瘤牛的血统。徐闻黄牛和海南黄牛mtDNA极低的遗传变异度表明两个品种的亲缘关系很近,从而在分子生物学水平为其合称为雷琼黄牛提供了佐证。 相似文献
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中国大陆若干群体的黑果蝇的线粒体DNA多态性研究 总被引:13,自引:2,他引:11
本文研究了果蝇D.virilis种群D.virilis线粒体DNA(mitochondrialDNA,mtDNA)的多态性。用9种限制性内切酶XbaⅠ,EcoRⅠ,PstⅠ,HindⅢ,BglⅡ,SacⅠ,ScaⅠ,EcoRV和PuvⅡ,对青岛、南京、上海、宁波与泉州5个D.virilis群体的mtDNA进行了限制性片段长度多态性(restrictionfragmentslengthpolymorphism,RFLP)的研究。在5群体中,发现5种不同的酶切图谱,它们彼此之间的遗传差异π为0.46%-1.76%,群体内遗传差异πij为0.00%-0.33%,群体间的差异dxy,为0.00%-0.82%。分布于中国大陆的D.virilis的群体间遗传差异在总遗传差异中所占比例γst值为24.62%。我们发现,D.virilis的栖息环境对mtDNA的遗传变异有十分明显的影响,而不同地理纬度的群体之间其遗传距离并无倾群(cline)表现。 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
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肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
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H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献