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A common plasmid of Chlamydia trachomatis 总被引:26,自引:0,他引:26
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柞蚕核型多角体病毒(ApNPV)转移载体质粒pAp M2614的组建 总被引:1,自引:0,他引:1
自从美国科学家G.Smith等首次建立苜蓿尺蠖核型多角体病毒(AcNPV)转移载体表达系统以来,已被广泛用于外源基因的表达,成为世界上一新的具有巨大潜力的载体表达系统。为了进一步提高表达产量,降低成本,日本科学家前田进建立了家蚕核型多角体病毒(BmNPV)载体表达系统,并获得了高效表达。柞蚕是我国特产,以蛹滞育越冬,保存时间长,个体大,可工厂化生产。因此,组建柞蚕NPV转移载体,进而建立该载体表达系统,是目前利用昆虫活体为宿主进行外源基因表达较理想的昆虫杆状病毒载体表达系统。 相似文献
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Plasminogenactivatorinhibitor1(PAI1)isaspecificphysiologicalinhibitorofurokinasetypeplasminogenactivator(uPA)andtissuetypeplasminogenactivator(tPA)[1].ChangesofPAI1mayinduceimbalancebetweenglomerularextracellularmatrix(ECM)synthesisanddegradation,thusleading… 相似文献
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Expression of the sulfonamide resistance gene from plasmid R46 总被引:5,自引:0,他引:5
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Virulence Plasmid-Borne spvB and spvC Genes Can Replace the 90-Kilobase Plasmid in Conferring Virulence to Salmonella enterica Serovar Typhimurium in Subcutaneously Inoculated Mice
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Hidenori Matsui Christopher M. Bacot Wendy A. Garlington Thomas J. Doyle Steve Roberts Paul A. Gulig 《Journal of bacteriology》2001,183(15):4652-4658
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A new vector, pUCPlac, was generated by introducing a truncated lacZ structural gene into pUChsneo [Steller and Pirrotta, EMBO J. 4 (1985) 167-171]. In front of this gene a new polylinker was added which will allow the in-frame fusion of sequences containing cis-acting regulatory elements plus translation start site of any given gene. After P-mediated germ-line transformation in Drosophila the action of these regulatory elements can be conveniently monitored by histochemical staining for beta-galactosidase activity. Correct cloning can be easily ascertained by supercoil plasmid sequencing of the constructs using commercially available primers. 相似文献
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